Malaria and the Blood-Brain Barrier

 EPCR and ICAM Receptors

Neuromics Astrocytes and Pericytes were used in this important study-Yvonne Adams, Rebecca W. Olsen, Anja Bengtsson, Nanna Dalgaard, Mykola Zdioruk, Sanghamitra Satpathi, Prativa K. Behera, Praveen K. Sahu, Sean E. Lawler, Klaus Qvortrup, Samuel C. Wassmer, and Anja T.R. Jensen. (2021). Plasmodium Falciparum Erythrocyte Membrane Protein 1 Variants Induce Cell Swelling and Disrupt the Blood–Brain Barrier in Cerebral Malaria. Journal of Experimental Medicine, 218 (3). doi: 10.1084/jem.20201266.

This is the first study showing malaria pericytes interacting with human endothelial cells. Please note we have the complete BBB Model that can be used for studies like these.

Customer Data Wanted

Reward is a $25 Amazon Gift Card
We are always honored when you share your data with us! To learn more check out the video.

We make it easy to share. Just email your data to rose@neuromics.com. We will email you the gift card.

Human Cells-utopia!

Great for use a controls vs Differentiated iPSCs

We have a cornucopia of Human Primary Neuron, Astrocytes and Schwann Cells plus CAFS. Featured Assays:
Human Brain Pericytes used to study Guide Axon Guidance.
Human Pancreatic CAFS and Tumor Dynamics.

Axon guidance at the site of a cervical spinal cord injury in a rat model. (Ai) Schematic illustrating transplantation of scaffold into a C-4 hemisection. The injury cavity is shown prior to (ii) and immediately following (iii) transplantation. (Bi) Scaffold conditioned with flow exhibits viable GFP-labeled microvessels (green) (ii) and alignment of host axons (magenta) infiltrating the scaffold in the rostral-caudal direction (grey arrow). (C) Scaffold conditioned in static conditions showing disrupted alignment of both microvessels (ii) and host axons (iii). (D–F) Microvessel and axon plots showing alignment (D,E) and length (F). Scale bars, 1 mm (Aii,Aiii) and 50 μm (B,C). Data are presented as mean ± s.e.m. ***P < 0.001; statistical significance was calculated using Welch Two Sample t-test. White arrows denote proximity of axons with microvessels. Microvessel alignment values (n = 30), axon alignment values (n = 30), microvessel length values (n = 15), and axon length values (n = 15) are from single hydrogel samples per condition.

Representative CAFs spheroids embedded in collagen gels at time 0 and at 6 h post implantation, respectively.

It is imperative that all our cells work as advertised. If your results do not meet expectations, we will run similar tests to make sure they walk and talk as they should.

We wish you and yours a Happy Holiday Season, Pete Shuster, CEO and Owner Direct phone: 612-801-1007 or pshuster@neuromics.com

Primary Human Astrocytes vs Derived Astrocytes Cell Lines

Potent, Pure and Easy to Culture

We are often asked how our human primary astrocytes stack up versus engineered astrocyte cell lines. Astrocyte cultures, whether primary or engineered, need to mimic how they work in-vivo.

This publication is a comprehensive study of the capabilities of our primary astrocytes versus engineered cells. Anders Lundin, Louise Delsing, Maryam Clausen, Piero Ricchiuto, José Sanchez, Alan Sabirsh, Mei Ding, Jane Synnergren, Henrik Zetterberg, Gabriella Brolén, Ryan Hicks, Anna Herland, and Anna Falk. (2018). Human iPS-Derived Astroglia from a Stable Neural Precursor State Show Improved Functionality Compared with Conventional Astrocytic Models. Stem Cell Reports. DOI: https://doi.org/10.1016/j.stemcr.2018.01.021.

With the exception of glutamate uptake, our primary cells talk and walk like astrocytes. We do plan on running assays to test for glutamate uptake as we believe the cells are capable.

It is important that all our human neurons, astrocytes, microglia and schwann cells are potent, pure and easy to culture. As you can see from this graphic, our "primary astros" our positive for most the required characteristics.

Human Astroglia and Schwann Cells-BBB Model

Broadening our Capabilities

At the core of our solutions are many options for primary human cells. We are especially pleased that we have growing capabilities to provide new cells to researchers studying autoimmune neuro-degenerative diseases like ALS and MS with the addition of:
Schwann Cells

Human Schwann Cells (HSwC) are isolated from human spinal nerve. HSwC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HSwC are characterized by immunofluorescence with antibodies specific to S100, GFAP, and CD90. HSwC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HSwC can to further expand for 10 population doublings in our Schwann Growth medium (cat # SGM001).
Human Astrocytes

 Human Brain Astrocytes cultured with AlphaBioCoat.
Human Blood Brain Barrier Model
I will continue to post updates here.

Human Astrocytes

Cortex Derived Astrocytes

Neuromics is pleased to be offering yet another option for culturing Human Astrocytes. They can be passaged up to 10X-Only 749 USD/500,000 Cells

They are deigned to be easy to culture and grow.

 Image: Human Brain Astrocytes cultured with our AlphaBioCoat.

Check out our large offering of Neurons, Astroglia, Progenitors, Brain Endothelial Cells/Pericytes and Blood Brain Barrier (BBB) Model. Should you have questions on these or any of our offerings, I can be reached directly at 612-801-1007 or pshuster@neuromics.com. Thank you, Pete Shuster-CEO and Owner.

Ready to Use Blood Brain Barrier Model (BBB)

6, 12 and 24 Well Options

We are pleased to introduce our BBB Model. It is designed to provide more in vivo like data for your drug discovery and toxicology assays.

Our model mimic transport properties of the BBB due to the formation of tight junctions, higher expression of specific carriers, or great cell viability. The model includes brain endothelial cells with pericytes and astrocytes layered in an insert. This model improves endothelial cell polarization and enhance the formation of tight junctions, provide better endothelial cell-to-cell contact that is important for barrier development, and prevent the dilution of secreted neurotrophic factors, and these conditions collectively led to the development of an in vitro model that can truly mimic the BBB.

Diagram of BBB Model Transporters

Our Protocol includes methods for Drug Penetration Asays. 

Transport of a molecule (blue) through 3D Human BBB Model in a luminal to basal direction 

Calculation of apparent permeability (Papp in cm/min)

Apparent permeability coefficient Papp (in cm/min) based on the Fick's law can be calculated according to the following equation:

V_A: volume of abluminal chamber (cm³)

A: membrane surface area (1.12 cm²)

[C]_L: initial luminal tracer concentration (ng/ml)

[C]_A : abluminal tracer concentration (ng/ml) t : time of experiment (min)

 Our goal is to provide you better data...at a lower cost.

GFP Labeled Motor Neurons and MEA

Big Upcoming Webinar

We have been strategically partnering with ArunA Biomedical to improve how we serve Neuro-drug discovery and Neurotox research community. I am especially excited about how well are our GFP Labeled Mouse Neurons are working in many different and unique research applications. They have proved an important tool in the study of neuro-muscular diseases like ALS, Parkinson's and Multiple Sclerosis.

I am pleased to announce a coming Webinar: “GFP+ Motor Neurons: Development and in-vitro Functional Assessment” Wednesday June 10th, 11:30 AM EDT-Register Today!

Download Flyer. I will continue to post unique assays developed for our Astro-glial Neuron solutions.