Tuesday, December 29, 2009

Tuj-1-Neuronal Differentiation Marker

Our Tuj-1 antibodies are widely used and frequently referenced in customer publications. They are proven markers for Neural Progenitor and Neuronal Differentiation. Here's the latest reference:

...Tuj 1 (Neuron-specific class III beta-tubulin)-Mouse (MO15013, Neuromics Antibodies, Edina, MN)...

Immunofluorescence Method:

Cells grown on coverslips were fixed for 5 min in 4% paraformaldehydecontaining 4% sucrose in phosphate buffer saline (PBS) at 37ºC. Cells were then permeabilized with 0.2% Triton X-100 in PBS during 5 min at room temperature. After blocking (5% bovine serum albumin in PBS for 1 h), cells were incubated with the corresponding primary antibodies, and immunoreactivity was detected with the suitable fluorophore-conjugated secondary antibody before mounting on slides with Mowiol4-88 (Harland Co., UK). Confocal images were acquired using an inverted Leica TCS SP5 laser confocal microscope with a 63X Plan- Achromatic oil immersion objective and processed with LAS AF Leica Application Suite and Adobe Photoshop CS2 (Adobe Systems
Inc., CA). All images correspond to the projection of sections from a ~50μm z-stack, except for colocalization analysis where they correspond to 0.5-0.7μm single sections.

Image: Rat hippocampal neurons were fixed at 1.5 DIV and immunostained for the neuronal marker βIII-Tubulin/Tuj1 (blue).

Thursday, December 24, 2009

Opioid Receptors and Depression

Tricyclic antidepressants (TCAs) have been reported to interact with the Opioid Receptor system, but their pharmacological activity at opioid receptors has not yet been elucidated.

We would like to share a recent publications that sheds more light on the mystery. Our purified rabbit ployclonal phosphoERK1/2 Antibody is also referenced.

Pierluigi Onali, Simona Dedoni and Maria C. Olianas. Direct Agonist Activity of Tricyclic Antidepressants at Distinct Opioid Receptor Subtypes. JPET January 2010 vol. 332 no. 1 255-265 .

At the cloned μ-opioid receptor, TCAs showed low affinity and no significant agonist activity. These results show that TCAs differentially regulate opioid receptors with a preferential agonist activity on either δ or κ subtypes and suggest that this property may contribute to their therapeutic and/or side effects.

Related Reagents:
phosphoERK1/2 (Rabbit MAb)
Immune Response Research Antibodies
Immune Response Research Proteins
Neurotrophins and Growth Factor Antibdodies
Neurotrophin Proteins
Pain and Inflammation Research Antibodies

Friday, December 11, 2009

Hope for Stroke Victims-Transplanting STEMEZ hNP1 Cells

In a recent publication (Jen et al., 2009) Neuromics'/ArunA’s STEMEZTM human neural progenitor (hNP1) cells when injected (sterotaxic) into a rat stroke model produced significant beneficial results. The hNP1 cells reduced the infarct area by 50% and were positive for neuronal marker proteins, cleaved caspase-3 and 40% of the cells showed spontaneous action potentials and excitatory postsynaptic currents measured by patch clamp recordings at 8 weeks post hNP1 cell injections. The treated rats had improves cognitive and sensorimotor functions between four to nine months post injection. These Sprague–Dawley rats were not immunosuppressed.

Kunlin Jin, XiaoOu Mao, Lin Xie, Veronica Galvan, Bin Lai, Yaoming Wang, Olivia Gorostiza, Xiaomei Wang and David A Greenberg. Transplantation of human neural precursor cells in Matrigel scaffolding improves outcome from focal cerebral ischemia after delayed postischemic treatment in rats. Journal of Cerebral Blood Flow & Metabolism advance online publication 14 October 2009; doi: 10.1038/jcbfm.2009.219.

STEMEZ(TM) hNP1 Human Neural Progenitors Discovery Kit
Other Reagents:
STEMEZ(TM) hN2 Human Neurons Discovery Kit
All Stem Cell Research Reagents
Primary Neurons and Astrocytes

Wednesday, December 09, 2009

New Mouse Monoclonal GFAP Antibody

Check it out!

GFAP Antibody

Excelent marker for human astrocyte intermediate filaments in the central nervous system. It has also been detected in the glial cells of the enteric nervous system and some Schwann cells in the peripheral nervous systems.

Posted using ShareThis

Saturday, December 05, 2009

Blood Pressure, Transient Receptor Potential Vanilloid 1 Receptors and Baroreceptors

Dr. Dr. Hui-Lin Pan (Department of Anesthesiology) and his team at M. D. Anderson Cancer Center has been a loyal user of our TRPV1 (VR1) since the early days of Neuromics' existence. We appreciate their continuing to use our reagent in their research.

Here's yet another publication:

Hao Sun, De-Pei Li, Shao-Rui Chen, Walter N. Hittelman and Hui-Lin Pan. Sensing of Blood Pressure Increase by Transient Receptor Potential Vanilloid 1 Receptors on Baroreceptors. doi:10.1124/jpet.109.160473

...VR1 C-terminus (TRPV1), dilution 1:1000, Neuromics...

Related Reagents:
All TRP Antibodies
Pain and Inflammation Research Antibodies
Neurotransmission -Neurotransmission Research Antibody Categories