Human Cardiomyocytes stained with Cardiac Tryponin T (green) and counter stained with DAPI (blue).
These cells are optimized to provide additional options for in-vitro testing of drug to drug candidate toxicities allowing researchers to rule out the ineffective and potentially toxic small molecules/compounds early in the process.
Immunohistochemical expression of serum response factor in rat dorsal root ganglia. Dorsal root ganglia dissected from male Sprague-Dawley rat and imaged by confocal microscopy for (A) TRPV1 (blue), (B) AKAP (AKAP150, green), and (C) SRF (red), with colocalization represented in panel (D). White arrows indicate cells expressing all 3 proteins. Yellow line represents 50 μM in length; images are representative of 4 individual sections taken from 6 L4 to L6 DRG. (E) Venn diagram of coexpression between SRF (red circle), AKAP (AKAP150, green circle), and TRPV1 (blue circle). (F) Percentages of SRF coexpression with AKAP (AKAP150) or TRPV1 calculated from 8 randomly selected coverslips. DRG, dorsal root ganglia; SRF, serum response factor.
Serum response factor mediates nociceptor inflammatory pain plasticity
PAIN Reports3(3):e658, May/June 2018.
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Immunofluorescent analysis of rat brainstem section stained with GT22102, MAP2 dilution 1:2,000 in red, and costained with mouse mAb to MO22121, MBP dilution 1:5,000, in green. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was postfixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies.
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