Thursday, June 28, 2018

You'll Love our FBS!

Check out Testimonials from Users
Neuromics is proud to offer you the high-quality Fetal Bovine Serum, the same serum we use internally, without the high cost. Our customers, researchers like you, agree.

Ordered Fetal bovine serum. Best price and quality" - Juwen D, Albert Einstein College of Medicine. Product: Heat-inactivated FBS, cat no. FBS001-HI

Outstanding service. Fetal bovine serum was of highest quality, easy to order and packaging and shipping were best possible." - Rosemary, University of Buffalo Product: Heat-inactivated FBS, cat no. FBS001-HI (see all of Neuromics reviews here).
Give our serum a try today! Neuromics US-Origin Fetal Bovine Serum is only 349USD/500ml. Heat-inactivated FBS only 364USD/500ml. Need a large quantity? Bulk discounts are available. Like a sample to try? Contact for a 50 ml sample.

Tuesday, June 26, 2018

Autophagy Assay Kits

Detects Autophagy in Living Cells
Autophagy is a conserved lysosomal recycling process by which cells break down their own components such as proteins, lipids, and carbohydrates. The process plays an important role in maintaining homeostasis and destroying intracellular pathogens. In addition, autophagy can be upregulated in times of starvation or stress to provide additional nutrients for the cell. Dysregulation of autophagy has implications for cancer, infection, and degenerative diseases.

Autophagy is a three-stage process. First, cytoplasmic components targeted for degradation are sequestered, resulting in the formation of the autophagosome. Next, the autophagosome fuses with the lysosome to form the autophagolysosome or autolysosome. Finally, degradation of the autophagosomal contents occurs.

Our Autophagy Assay, Red (cat# KF17373) enables researchers to detect and monitor the in vitro development of autophagy in living cells. The Autophagy Probe is cell-permeant and fluoresces red when inserted in the lipid membranes of autophagosomes and autolysosomes. Results can be read using a flow cytometer.
Figure. Flow Cytometry Results. Autophagy Assay Kit, Red was used to assess the induction of autophagy in Jurkat cells. Cells were either untreated (Black) or treated with 0.5 μM Rapamycin (Orange), 10 μM Chloroquine (Blue), or both 0.5 μM Rapamycin and 10 μM Chloroquine (Red) for 18 hours. After staining with Autophagy Probe, Red for 60 minutes, cells were washed and analyzed by flow cytometry (BD LSRFortessa Special Order flow cytometer equipped with a green/yellow laser (561 nm excitation) and a 610/20 emission filter). An overlay of the histograms is shown on the right. A table displaying the median fluorescence signal, % negative, and % positive cells is shown below. Treatment with Rapamycin or chloroquine increased the fluorescence signal detected compared to the untreated control. Combined treatment of rapamycin and chloroquine further increased the fluorescence signal detected. Data courtesy of Dr. Kristi Strandberg (ICT 228:37-40).

We have many options for detecting apoptosis, necrosis, autophagy, and cytotoxicity in living cells. 

Thursday, June 14, 2018

Markers for Tyrosine Hydroxylase-TH

Proven and Published
Tyrosine hydroxylase TH is a necessary enzyme to create neurotransmitters and protect the body against oxidative stress.

Dysregulation of this enzyme results in manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.

Markers to TH are important for studying its role in neurotransmission and how it is altered in disorders and diseases.

We have many options and this enables you to choose the best markers for your research. All are proven and published.
TH staining of mouse salivary gland tissue.
Check out all our markers for neuroscience research.

Wednesday, June 06, 2018

Put our Antibodies to Work for You!

Tested and Characterized for Results You Can Trust
When you select a vendor for antibodies, there is a measure of trust. We know you are asking, "will it work in the applications as advertised".

Since the inception of Neuromics, our guiding principle is providing well-characterized solutions for results you can trust and understand. In order to provide as much comfort in the purchase of our solutions, we encourage access to publications, data, and testimonials.

Here're some recent testimonials:
  • Good antibody! Product Name: Tuj 1, Chicken – (Cat# CH23005) Good for neuronal staining. Bright and specific.Liang Shi - May 03, 2018 - Rating: 5.0 
  • Neuromics has a conjugated antibody for oligodendrocytes that is unavailable anywhere else and works really well. Very pleased with the product and have already ordered it a few times. Edit: This antibody is available from Miltenyi now. They both behave comparably. Matthew Smith - Apr 24, 2018 - Rating: 5.0 
  • I bought the secondary antibodies (488-Goat anti mouse IgG and 546-goat anti rabbit IgG) from Neuromics and used them for IF assay. Both antibodies worked well. I highly recommend them.Bonnie Dai - Apr 21, 2018 - Rating: 5.0 
Expression of TH (green) and TRPV1 (blue) relatively to CGRP-cre+ neurons (red) in DRG from CGRPcre-ER/+;Rosa26LSL-tDTomato/+ mice. Some TRPV1+ DRG neurons are not expressed in CGRP-cre+ sensory neurons. These CGRP-cre-/TRPV1+ neurons are marked with yellow arrows (a and a1). b. Expression of calbindin-28K (Calb; green) relatively to CGRP-cre+ DRG neurons (red). Yellow arrows mark CGRP-cre-/Calb+ neurons (b and b1). c. Expression of trkB (green) relatively to CGRP-cre+ DRG neurons (red). Yellow arrows mark CGRP-cre-/trkB+ neurons; and a blue arrow shows a rare example of the CGRP-cre+/trkB+ neuron (c and c1). d. Expression of trkC (green) relatively to CGRP-cre+ DRG neurons (red). Yellow arrows mark CGRP-cre-/trkC+ neurons; and; and a blue arrow shows an example of the CGRP-cre+/trkC+ neuron (d and d1). White horizontal bar shows 20μm scale for each panel.

We offer an easy, "no question asked" replacement or refund policy. Give us a try. You will be satisfied.

Monday, June 04, 2018

New Fibrillarin Antibody

Potent Nucleoli Marker
Given the importance of Nucleus and Related Markers to all branches of cell/molecular biology, we are dedicated to having the best.

We have just added a new Fibrillarin Antibody.
Images: Immunofluorescent analysis of (A) C6 rat glioma cells and (B) Human embryonic kidney cells stained with mouse mAb to fibrillarin, MO22169, dilution 1:1,000 in red, in both cases costained with chicken pAb to vimentin, CPCA-Vim, dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA.

Nop1p/Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli. Nop1p is the yeast homolog of a protein apparently found in all eukaryotes and archaea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6). The HGNC name for this protein is FBL. To raise the MCA-4H4 antibody, mice were injected with full length recombinant human fibrillarin.