About IBA Lifesciences

IBA Lifesciences' proprietary Strep-tag® technology exploits one of the strongest non-covalent interactions in nature: the interaction of biotin and streptavidin. The system is based on the highly selective and easily controllable interaction between the synthetic Strep-tag®II peptide and the specially engineered streptavidin, called Strep-Tactin®, which is one of the most stable proteins known. The Strep-tag®II binds specifically to the engineered streptavidins, Strep-Tactin® and Strep-Tactin®XT, by occupying the binding pocket of the natural ligand biotin. Hence, the interaction is easily reversible by excessive addition of the competitor.

Strep-tag®II consists of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys), whereas the Twin-Strep-tag® includes this motif two times in series connected by a linker and is accordingly composed of 28 amino acids. Both exhibit intrinsic, although unequal, affinity towards the streptavidin derivative Strep-Tactin® and its successor Strep-Tactin®XT: The binding affinity of Strep-tag®II to Strep-Tactin® (1µM) is nearly 100 times higher than to streptavidin. A further improvement was achieved by the development of Strep-Tactin®XT, which shares a nM affinity with the Strep-tag®II and a pM affinity with the Twin-Strep-tag®.

To Learn more watch this intro video/

Studying Diabetic Retinopathy?

Check out How Neuromic's Tools Are Used

The authors used four of Neuromics to complete this study-

Sulodexide reduces glucose induced senescence in human retinal endothelial cells A. Gericke, K. Suminska-Jasińska & A. Bręborowicz Scientific Reports volume 11, Article number: 11532 (2021) Cite this article.

Material and Methods

Experiments were performed on HREC (#HEC09, Neuromics, Edina, MN, USA) in in vitro culture. Cells were seeded in 75 cm² culture flasks coated with AlphaBioCoat Solution (#AC00, Neuromics, Edina, MN, USA) and were grown in Endo Growth Medium (EKG001, Neuromics, Edina, MN, USA) supplemented with fetal bovine serum 0.5% until they formed monolayers. Then the cells were harvested with cell detachment solution (#ADF001, Neuromics, Edina, MN, USA) and seeded in quadruplicates into 25 cm² coated, as described above, culture flasks at a density of 1.5 × 10⁵ cells/flask.

We conclude that Sulodexide may have a beneficial effect in cases of diabetic retinopathy. It slows down hyperglycemia-dependent senescence of endothelial cells, which translates into the lower angiogenic and inflammatory impact of these cells. An important observation was that Sulodexide also has effective antiangiogenic and anti-inflammatory effects in the senescent HREC. That means that Sulodexide may be effective in retinal endothelial cells, which are already senescent. Further studies are required to explain the potential effect of Sulodexide on the endothelial glycocalyx structure and permeability of the retinal endothelial layer composed of the senescent cells to molecules with various size and electrical charge.

We are pleased to see our solutions used in this important  study. It could lead to drugs that slow diabetic retinopathy.

Neuromics' FBS and National Cancer Institutes

 Best Price and Performance 

I was honored to work with Jessey at National Cancer Institute (NCI).

The were determining whether to change partners. According to Jessey, there were 24 lab PIs who would be supplied with the winner's FBS Although we proved competitive, we were not selected.

The study was conducted with only premium grade FBS (most expensive). We are announcing a new Preferred FBS this Monday 10/25. It works well for supplementing define media and works great for most human primary sells. And works fabulous for human and animal cancer cells.

Interested in learning more? Contact me at pshuster@neuromics.com or 612-801-1007. Pete Shuster CEO, Neuromics.

Neuromics-3D Human Blood Brain Barrier!

Compound Penetration Study

  

Classification: Low permeability: Pe < 1.00 nm/s 

Moderate permeability: 1.00 < Pe < 10.0 nm/s 

High permeability: Pe > 10.0 nm/s 

* The signal responses of Atenolol , 

Sulpiride, and Cimetidine in receiver samples were undetectable.

For the convenience of calculation, 

1/300 of the measured peak area ratio (PAR) of the relevant AD (A>B Donor) 

samples were used as the PAR values of Atenolol , Sulpiride, and Cimetidine 

in receiver samples.

Check out the study-

https://www.neuromics.com/ittrium/reference/D8xf65cx8x1/BBB%20Penetration%20Data.pdf  

Neuromics' Renal Proximal Tubule Cells in Action

Human Renal Proximal Tubule Cells Express Klotho

Our human primary cells are widely used and frequently referenced in a variety of publications. In this study, our RPT Cells were used to study the impact of Klothko expression on protection of kidneys in diabetics: Meng Xue, Feng Yang, Ying Le, Yanlin Yang, Bingsen Wang, Yijie Jia, Zongji Zheng, and Yaoming Xue. (2021). Klotho Protects Against Diabetic Kidney Disease via AMPK- and ERK-Mediated Autophagy. Acta Diabetologica, 32. doi: 10.1007/s00592-021-01736-4.

Klotho expression in human renal proximal tubule cells after high-glucose stimulation. (a) Levels of Klotho mRNA and (b) Klotho protein were assessed in human renal proximal tubule cells treated with low glucose and high glu- cose. (c) Morphological observations of Klotho expression by confocal microscopy

Klotho improved renal tubular cell autophagy via the AMPK and ERK path- ways and played a role in renal protection. These findings provide new insight into the mechanism of Klotho and autophagy in DKD.

Trigeminal Neuralgia (Facial Pain)

 Markers for Pain Research

Trigeminal Neuralgia is a progress chronic condition. Dysfunctional trigeminal signaling can lead to intense, searing facial pain. It is caused by pressure on the trigeminal nerve.

The root causes of the condition are not well understood. Our pain research markers have been widely used and frequently published through the years and have been important contributors to our growth, Here's a recent pub referencing use of one our P2X3 Antibodies-Momoko Koizumi, Sayaka Asano, Akihiko Furukawa, Yoshinori Hayashi, Suzuro Hitomi, Ikuko Shibuta, Katsuhiko Hayashi, Fusao Kato, Koichi Iwata, and Masamichi Shinoda. (2021). P2X3 Receptor Upregulation in Trigeminal Ganglion Neurons Through TNFα Production in Macrophages Contributes to Trigeminal Neuropathic Pain in Rats. The Journal of Headache and Pain, 22, 31. doi: 10.1186/s10194-021-01244-4

Images: Changes in P2X3R expression in TG neurons innervating whisker pad skin and the involvement of P2X3R signaling in TG neurons in orofacial mechanical hypersensitivity on day 7 following TNC. a Photomicrograph of FG-labeled P2X3IR TG neurons following the TNC or sham procedure. Arrows indicate FG-labeled P2X3IR TG neurons. Scale bar: 100 μm. b Mean number of FG-labeled TG neurons ipsilateral to the TNC or sham procedure (n = 5 in each). c The frequency of FG-labeled P2X3-IR TG neurons ipsilateral to the TNC or sham procedure. * p < 0.05 vs. sham. (n = 5 in each, student’s t-test). d The time course of changes in the MHWT by subcutaneous A317491 or TNP-ATP administration on day 7 following the TNC or sham procedure.

The signaling of TNFα released from the activated macrophages and infiltrated into and/or proliferated in the TG induces the upregulation of P2X3R expression in TG neurons innervating the orofacial region, resulting in orofacial mechanical allodynia following TNC. Consequently, TG neuronal P2X3R hyperexpression by enhanced TNFα signaling in the TG is a potential target for TN treatment..

Malaria and the Blood-Brain Barrier

 EPCR and ICAM Receptors

Neuromics Astrocytes and Pericytes were used in this important study-Yvonne Adams, Rebecca W. Olsen, Anja Bengtsson, Nanna Dalgaard, Mykola Zdioruk, Sanghamitra Satpathi, Prativa K. Behera, Praveen K. Sahu, Sean E. Lawler, Klaus Qvortrup, Samuel C. Wassmer, and Anja T.R. Jensen. (2021). Plasmodium Falciparum Erythrocyte Membrane Protein 1 Variants Induce Cell Swelling and Disrupt the Blood–Brain Barrier in Cerebral Malaria. Journal of Experimental Medicine, 218 (3). doi: 10.1084/jem.20201266.

This is the first study showing malaria pericytes interacting with human endothelial cells. Please note we have the complete BBB Model that can be used for studies like these.

Human Schwann Cells that Work

 No Doubt!

Sometimes we hear our human cells did not express a certain receptor in the hands of our customers. We guarantee our cell "walk and talk" as advertised. If you are ever in doubt. we will test for expression of any receptors or cytoplasmic proteins. We also do this as part of our quality checks.

Here're, for example, is a pub showing our Schwann Cells working. Note the receptors tested here. Seung Min Shin, Francie Moehring, Brandon Itson-Zoske, Fan Fan, Cheryl L. Stucky, Quinn H. Hogan, and Hongwei Yu. (2021). Piezo2 Mechanosensitive Ion Channel is Located to Sensory Neurons and Non-Neuronal Cells in Rat Peripheral Sensory Pathway: Implications in Pain. bioRxiv. doi: 10.1101/2021.01.20.427483

Figure. IHC delineation of Piezo2 (PZ2) axonal component and Schwann cell expression. Representative montage images on sciatic nerve cryosections show double immunostaining (IS) of Piezo2 (red) with a selection of neuronal markers (green), including Tubb3 (A), NF200 (B), IB4 (C), and CGRP (D). Representative montage images on sciatic nerve cryosections show double- IS of Piezo2 (red) with glia markers (green), including S100 (E), p75NTR (F), GFAP (G), and MBP (H). Representative montage images on human Schwann cells (I) and isolated Schwann cells (J) from rat sciatic nerve show double-IS of Piezo2 (red) with S100 (green). Scale bars: 50 um for all.

Check out our growing catalog of primary human cells today https://www.neuromics.com/human-cells-tissue. Satisfaction guaranteed or your money back.