Wednesday, January 27, 2010

Nucleostemin-Stem Cell Marker

Our Nucleostemin Antibody has proven a versatile Stem Cell Marker.

Researchers have referenced use of this antibody as a marker for Neural Progenitors and Muscle-Derived Stem Cells. In the most recent of our Nucleostemin Publications, Dr James Wang (University of Pittsburgh) references using the antibody for staining Tendon Progenitors/Stem Cells (TSCs):

Jianying Zhang and James H-C. Wang. Characterization of differential properties of rabbit tendon stem cells and tenocytes. BMC Musculoskeletal Disorders. 2010, 11:10doi:10.1186/1471-2474-11-10.
...The staining protocol used goat anti-human Nucleostemin Antibody (1:300; Neuromics, Cat. No. GT15050) and Cy3-conjugated donkey anti-goat IgG secondary antibody...

Image: Achilles Tendon Stem Cells (ATSCs) expressed nucleostemin. Insets show enlarged view of expressed nucleostemin in pink (bar: 50 μm).

Related Reagents:
Stem Cell Markers
All Stem Cell Research Reagents
Primary Neurons and Astrocytes-Primary human, rat and mouse neurons and astrocytes

Tuesday, January 19, 2010

i-Brite Plus!

We are all for reagents that brighten your day.

i-BRITE Plus is a glycerol based liquid. It can be used to stain cells and can be easily added to wells. It also will not shrink tissue. In addition standard to IHC/IF applications, it can be used to visualize GFP transfections and more.

Here's a publication referencing it: Ajay S. Yekkirala, Alexander E. Kalyuzhny and Philip S. Portoghese. Standard Opioid Agonists Activate Heteromeric Opioid Receptors: Evidence for Morphine and [d-Ala2-MePhe4-Glyol5]Enkephalin as Selective μ−δ Agonists. ACS Chem. Neurosci., Article ASAP DOI: 10.1021/cn9000236. Publication Date (Web): November 25, 2009. Copyright © 2009 American Chemical Society.

After that cells were washed in PBS (3 × 15 min), counterstained with DAPI and mounted under coverslips with antifade mounting media iBright Plus (cat. no. SF40000-10; Neuromics, Inc.). Images of labeled cells were collected using Olympus FluoView1000 confocal microscope.
Images: High-magnification confocal images of double-labeling immunofluorescence for HA-δ and FLAG-μ opioid receptors. HEK-293 cells stably expressing both HA-δ and FLAG-μ opioid receptors are shown labeled for μ (A, green fluorescence) and for δ (B, red fluorescence). DAPI (blue fluorescence) has been used to stain the nuclei.