Tuesday, February 09, 2016

Ready to Use Blood Brain Barrier Model (BBB)

6, 12 and 24 Well Options

We are pleased to introduce our BBB Model. It is designed to provide more in vivo like data for your drug discovery and toxicology assays.

Our model mimic transport properties of the BBB due to the formation of tight junctions, higher expression of specific carriers, or great cell viability. The model includes brain endothelial cells with pericytes and astrocytes layered in an insert. This model improves endothelial cell polarization and enhance the formation of tight junctions, provide better endothelial cell-to-cell contact that is important for barrier development, and prevent the dilution of secreted neurotrophic factors, and these conditions collectively led to the development of an in vitro model that can truly mimic the BBB.
Diagram of BBB Model Transporters
Transport of a molecule (blue) through 3D Human BBB Model in a luminal to basal direction 
Calculation of apparent permeability (Papp in cm/min)
Apparent permeability coefficient Papp (in cm/min) based on the Fick's law can be calculated according to the following equation:
VA: volume of abluminal chamber (cm3)
A: membrane surface area (1.12 cm2)
[C]L: initial luminal tracer concentration (ng/ml)

[C]A : abluminal tracer concentration (ng/ml) t : time of experiment (min)

 Our goal is to provide you better data...at a lower cost.

Sunday, January 24, 2016

More and More OR Pubs

Potent, Proven and Frequently Published

Many labs have used our Opioid Receptor antibodies over the past 10+ years. We are grateful for the many publications and testimonals referencing successful use of these antibodies.

Here's a sampling of more recent publications: Wendy M. Walwyn, Wenling Chen, Hyeyoung Kim, Ani Minasyan, Helena S. Ennes, James A. McRoberts, and Juan Carlos G. Marvizón. Sustained Suppression of Hyperalgesia during Latent Sensitization by μ-, δ-, and κ-opioid receptors and α2A Adrenergic Receptors: Role of Constitutive Activity. The Journal of Neuroscience, 6 January 2016, 36(1): 204-221; doi: 10.1523/JNEUROSCI.1751-15.2016
...probed with antibodies to MOR (RA10104; Neuromics), p-Ser375-MOR (pMOR, RA18001; Neuromics) and p-Tyr416-Src family kinase (pSFK...serine-phosphorylated MOR (p-Ser375-MOR, RA18001; Neuromics) and a different MOR antibody (RA10104...
Jan Fraessdorf, Markus W. Hollmann , Iris Hanschmann, André Heinen, Nina C. Weber, Benedikt Preckel, Ragnar Huhn. Role of Endogenous Opioid System in Ischemic-Induced Late Preconditioning. Published: July 30, 2015DOI: 10.1371/journal.pone.0134283.
...Antibodies for μ-OR and δ-OR were purchased from Neuromics (Minneapolis, USA)...
Ying-Biao Chen, Fen-Sheng Huang, Ban Fen, Jun-Bin Yin, Wei Wang and Yun-Qing L nhibitory effects of endomorphin-2 on excitatory synaptic transmission and the neuronal excitability of sacral parasympathetic preganglionic neurons in young rat. Journal Name: Frontiers in Cellular Neuroscience, ISSN: 1662-5102, Article type: Original Research Article,Received on: 02 Apr 2015, Accepted on: 12 May 2015, Provisional PDF published on: 12 May 2015.
... guinea pig antiserum against MOR (1:1000, GP10106, Neuromics, Edina, Minnesota, USA)...
Sherika N. Smith, Candler Paige, Kandy T. Velazquez, Terika P. Smith, Srinivasa N. Raja, Steven P. Wilson, Sarah M. Sweitzer. Injury-specific promoters enhance herpes simplex virus mediated gene therapy for treating neuropathic pain in rodents. doi:10.1016/j.jpain.2014.12.007
...: MOR (Neuromics, RA10104, 1:1000)...

Figure: MOR in primary sensory neuron and its terminals were decreased in neuropathic mice. (A, B) Western blot shows that MOR protein were markedly decreased in sciatic nerve (A) and hindpaw skin (B) on day 7 after nerve injury when compared with sham-operated mice. The ratio of MOR/GAPDH in sham mice was set at 1 for quantifications. Zhou et al. Molecular Pain 2014, 10:51 http://www.molecularpain.com/content/10/1/51

We will continue to post information on our ORs.

Thursday, January 14, 2016

Why You Should Consider Culturing Your Cells in 3-D

2-D versus 3-D and meaningful outcomes

We have seen recent growth in our customers' demand for 3-D cell based assay solutions. This could be due to more the fact that these assays our proving to generate more in vivo like data.

Breast cancer cells, for example, grown in 2-D can easily be killed by low doses of chemotherapeutic drugs or low doses of radiation. If those same cells are grown in 3-D, they are resistant to the same doses of chemotherapeutic drugs or radiation, just like cancer as its found in the body. In this way, cells grown in 3D are more valid targets for testing and discovering new drugs to treat cancer. Another benefit of testing drugs in three dimensional cell culture versus two dimensional cell culture is that cells in 3D form multi-layers of cells whereas cells grown in 2-D form a monolayer of cells that is spread very thin on a plastic surface. When testing a drug in 2-D, it needs only to diffuse a short distance across the cell membrane to reach its intended target. In 3-D, the situation is more realistic and a drug needs to diffuse across multi-layers of cells to reach the cells on the inside of a microtissue.

Neuromics has a variety of options that will enable you to choose the best option(s) for your 3-D assays. These include:
Look for more posting on these important solutions.

Saturday, January 09, 2016

Sleeping Beauty (SB) Transposon for the Study of Cancers

SB Gene Insertion vs Viral Based Methods

With the addition of SB Transposon Systems to our tool set. We have deep interest in how well SB works vs alternative methods. Here Dr. Hyun-Pyo Kim and team demonstrate how the SB Transposon system can be used for Novel Therapeutic Approaches for Various Cancer Types Using a Modified Sleeping Beauty-Based Gene Delivery System (DOI: 10.1371/journal.pone.0086324).

SB vs Viral Based Methods: "Successful gene therapy largely depends on the selective introduction of therapeutic genes into the appropriate target cancer cells. One of the most effective and promising approaches for targeting tumor tissue during gene delivery is the use of viral vectors, which allow for high efficiency gene delivery. However, the use of viral vectors is not without risks and safety concerns, such as toxicities, a host immune response towards the viral antigens or potential viral recombination into the host's chromosome; these risks limit the clinical application of viral vectors. The Sleeping Beauty (SB) transposon-based system is an attractive, non-viral alternative to viral delivery systems. SB may be less immunogenic than the viral vector system due to its lack of viral sequences. The SB-based gene delivery system can stably integrate into the host cell genome to produce the therapeutic gene product over the lifetime of a cell. However, when compared to viral vectors, the non-viral SB-based gene delivery system still has limited therapeutic efficacy due to the lack of long-lasting gene expression potential and tumor cell specific gene transfer ability. These limitations could be overcome by modifying the SB system through the introduction of the hTERT promoter and the SV40 enhancer. In this study, a modified SB delivery system, under control of the hTERT promoter in conjunction with the SV40 enhancer, was able to successfully transfer the suicide gene (HSV-TK) into multiple types of cancer cells. The modified SB transfected cancer cells exhibited a significantly increased cancer cell specific death rate. These data suggest that our modified SB-based gene delivery system can be used as a safe and efficient tool for cancer cell specific therapeutic gene transfer and stable long-term expression."

Figure 8. The effect of modified SB system on the tumor growth in vivo. Lung cancer cells (H358) (A), prostate cancer cell line (DU-145) (B), and ovarian cancer cells (OVCAR3) (C) were harvested by trypsinization, and 1×105 viable cells (as determined by trypan blue exclusion) in a total volume of 200 µl were injected subcutaneously. Two days following tumor seeding, animals were intravenously injected via tail veins with 100 mg/kg gancyclovir (GCV) along with either co-transfection of the empty plasmid (pT. hTp. Con) with the active helper plasmid (pCMV-SB) or co-transfection of the SB system (pT.hTp.HSV-tk.Con) with the active helper plasmid (pCMV-SB). Mice were sacrificed 28 days after tumor injection, and the effect of modified SB system on tumor growth was evaluated by measuring tumor size. doi:10.1371/journal.pone.0086324.g008

Neuromics, with our partner B-Mogen, has the capabilities to engineer custom SB Transposons for your Cancer Research. If interested, please contact me directly at 612-801-1007 or pshuster@neuromics.com. Our process is to first completely understand your unique requirements and from these, formulate a related statement of work with costs, timeline, milestones and deliverables. Thank you. Pete Shuster, CEO and Owner,

Sunday, January 03, 2016

CRISPR-Cas9 and Sleeping Beauty Transposons

Neuromics-B-Mogen Approach

The CRISPR-Cas9 gene editing approach is now officially considered a revolution. This is confirmed by the increasing frequency of this word being used across the web in describing this technique. In fact, it is now appearing in conference titles. see: Genome Engineering: The CRISPR/Cas Revolution 2016COLD SPRING HARBOR - 17 AUG 2016.

We have joined the revolution with our new Sleeping Beauty TransposonTM Systems.
Figure: Sleeping Beauty TransposonTM Systems Gene Integration Process. (A) A depiction of Sleeping Beauty transposon plasmid and Sleeping Beauty transposase enzyme active in cellular nuclei. (B) Transposase enzyme binds to Sleeping Beauty-specific IR/DR sites. (C) Transposase enzyme excises transposon sequence from transposon vector. (D) Transposase enzyme stably integrates transposon sequence at TA site in host cell genome.

Features Include:
  • Simple-"Cut and Paste" Integration and Editing. Check out our Sleeping Beauty Transposon System Users Manual 
  • Potent-100% Stable with No Off target effects with the safest transposon insertion profile. 
  • Fast- Transfect with our transposase + Sleeping Beauty Transposon Reporter Series Vectors. 
  • Cost Effective- Starting at $329.
If you would like us to engineer custom Transposons or gene editing on your cells, please conatact me directly and we will work together to formulate a Statement of Work to your specifications. Pete Shuster, CEO and Owner, Neuromics, pshuster@neuromics.com or cell phone: 612-801-1007

Thursday, December 17, 2015

Fibroblasts into Neurons

Via Activation of Oct4

We have a variety of stem cell, progenitor and neuron markers that are often referenced in publication. There represent a qualitative way to determine the state of stem cells as they differentiate.

Here're researchers take Fibroblasts and differentiate them into various progenitors. They were able to further differentiate these progenitors into astrocytes and neurons by: For neuronal differentiation, after 2 days of incubation with 20 nM reversine, cells were then treated with 0.5 μM all-trans-retinoic acid (RA) in serum-free DMEM/F-12 medium supplemented with the ITS for 2 days and switched into serum-free medium in the absence of RA with replacement of the medium every 2-3 days. 

Our NSE and GFAP were used to confirm this differentiation.
To learn more see: Xiangchen Li, Yu Guo, Yaxin Yao, Jinlian Hua, Yuehui Ma, Changqing Liu, Weijun Guan.Reversine Increases the Plasticity of Long-Term Cryopreserved Fibroblasts to Multipotent Progenitor Cells through Activation of Oct4. International Journal of Biological Sciences 2016; 12(1): 53-62. doi: 10.7150/ijbs.12199

Wednesday, December 09, 2015

Kv Channels and Pain Transmissiom

i-Fect TMis a Proven Tool for Gene Manipulation in Studying All Types of Pain

I previously posted on use of our i-Fect Transfection Kit to silence Kv Channels Receptors. This has enabled researchers to study the role of these receptors in vitro and in vivo (see:i-Fect™ Delivers Your siRNA Payload).

Sample Data

Figure: Figures. siRNA-mediated knockdown of Kv1.1 expression in thoracic DRG significantly increased gastric sensitivity in naive adult rats. (A) Western blots showed a significant decrease in Kv1.1 protein in thoracic DRG (T8–T12) after intrathecal treatment with Kv1.1 siRNA but not with control siRNA. siRNA treatment did not alter TrpV1 expression (n = 5 rats each; *P < .01 vs control siRNA). (B) Naive rats treated with Kv1.1 siRNA showed a significant increase in VMR to gastric distention (n = 5 rats each, compared with pretreatment baseline; *P < .05). (C) Treatment with control siRNA had no significant effect on gastric hypersensitivity. (D) Patch clamp recordings from freshly dissociated gastric DRG neurons from FD-like and PND 10 saline-treated littermate controls showed a significant decrease in rheobase in FD-like rats (*P < .05), and (E) a significant increase in the number of action potentials elicited by current injection at 3× the rheobase in gastric DRG neurons from FD-like rats (*P < .05). (F) Sample voltage vs time traces showing action potentials evoked at ×1, ×2, and ×3 rheobase. The patch clamp data were obtained from 16 cells from 5 PND 10 saline control rats and 19 cells from 5 FD-like rats

I am pleased to share with you a new reference detailing how research use i-Fect to optimize and deliver euchromatic histone-lysine N-methyltransferase-2 (G9a) siRNA. This brings the number of publications referencing use of our Transfection Kits to over 45: Geoffroy Laumet, Judit Garriga, Shao-Rui Chen, Yuhao Zhang, De-Pei Li, Trevor M Smith, Yingchun Dong, Jaroslav Jelinek, Matteo Cesaroni, Jean-Pierre Issa & Hui-Lin Pan G9a is essential for epigenetic silencing of K+channel genes in acute-to-chronic pain transition. Nature Neuroscience (2015) doi:10.1038/nn.4165.

The authors report: "Selective knockout of the gene encoding G9a in DRG neurons completely blocked K+ channel silencing and chronic pain development after nerve injury. Remarkably, RNA sequencing analysis revealed that G9a inhibition not only reactivated 40 of 42 silenced genes associated with K+ channels but also normalized 638 genes down- or upregulated by nerve injury."

I will continue to post here new and unique solutions and related referencing for our Gene Expression Analysis Tools.

Saturday, November 28, 2015

Neuron Astrocyte Glial Markers

Potent and Frequently Published!

We have a stout catalog of Neuron-Astrocyte and Glial Markers.

They are widely used and frequently published. Here're some recent examples:
Mouse Monoclonal GFAP: Chelsea M. Larabee, Constantin Georgescu, Jonathan D. Wren and Scott M. Plafke. Expression profiling of the ubiquitin conjugating enzyme UbcM2 in murine brain reveals modest age-dependent decreases in specific neurons. BMC Neuroscience201516:76 DOI: 10.1186/s12868-015-0194-y© Larabee et al. 2015.

Image: Mixed neuron-glial cultures stained with Mouse Monoclonal GFAP, and Chicken Polyclonal Neurofilament-NF-L (green). The GFAP antibody stains the network of astrocytes in these cultures, while the NF-L antibody stains neurons and their processes. The blue channel shows the localization of DNA. This antibody also works on formalin fixed paraffin embedded brain tissues. Protocol on Datasheet.

Tuj-1: Gaoying Sun, Wenwen Liu, Zhaomin Fan, Daogong Zhang, Yuechen Han, Lei Xu1, Jieyu Qi, Shasha Zhang, Bradley T. Gao, Xiaohui Bai,Jianfeng Li,Renjie Chai, Haibo Wang. The three-dimensional culture system with matrigel and neurotrophic factors preserves the structure and function of spiral ganglion neuron in vitro.

Need more assurances? Here're some feedback highlights.

STEPHEN C. Nov 19, 2015 Staining (Tuj1) worked well on human neural progenitor cells. Product Name: Tuj 1, Mouse – (Cat# MO15013-100) http://bit.ly/1Qx4ASc Organization: UCONN
GINA D. Oct 07, 2015 Very nice antibody and ordering is very easy using the website. Product Name: proDynorphin (rat), Guinea Pig – (Cat# GP10110) http://bit.ly/Sw4RJ9 Organization: Rosalind Franklin University
CAROL Feb 05, 2015 We got antibodies and received them fast with a correct temperature. Product Name: Coronin 1A, Chicken – (Cat# CH23017) http://bit.ly/1AxduYvProduct Name: Integrin alpha-M, Chicken – (Cat# CH23021)http://bit.ly/16lMihU Organization: UCSF

We have a full money back guarantee so do not hesitate to consider these markers for your assays.

Friday, November 20, 2015

Staining Neurons and 3-D

Tuj-1 or Beta Tubulin Antibody in Action!

Our potent Neuron-Glia Markers are widely used and frequently published. We are also pleased with the many positive reviews.

In this study researchers use our Tuj-1/Beta Tubulin Antibody to stain neurons in 3-D Cultures: Gaoying Sun, Wenwen Liu, Zhaomin Fan, Daogong Zhang, Yuechen Han, Lei Xu1, Jieyu Qi, Shasha Zhang, Bradley T. Gao, Xiaohui Bai,Jianfeng Li,Renjie Chai, Haibo Wang. The three-dimensional culture system with matrigel and neurotrophic factors preserves the structure and function of spiral ganglion neuron in vitro...Tuj-1-β-tubulin (1:1000, Neuromics, USA)...

Images: Morphology of the SGNs growth cone cultured in 2D and 3D systems. Phalloidin, green; β-tubulin, red.

I will continue to post positive developments concerning use of our Neuron-Glia Markers.

Wednesday, November 11, 2015

Reconstructing 3D Living Biomaterials

Hydrogel Solutions for Tissue Reconstruction and Repair

Alphabioregen/Neuromics are pleased to announce the use of our Hydrogels for reconstructing living biomaterials for regenerative biology and medicine: Suwan N. Jayasinghe,Jensen Auguste, Chris J. Scotton. Platform Technologies for Directly Reconstructing 3D Living Biomaterials. DOI: 10.1002/adma.201503001.

In this study, researchers use our  Collagen Hydrogel, Collagen Hydrogel Soft and Collagen Hydrogel Soft+  to describe  in vivo applications using a murine model to interrogate biocompatibility and cellular behavior post-transfer.

Luc macrophages (CC or BES) were then incorporated into a fl uid mixture containing one of three alternative collagen-based hydrogels, namely, Collagen Hydrogel, Collagen Hydrogel Soft, or Collagen Hydrogel Soft+ (resulting in six possible combinations of macrophages and biopolymer).

Overview of Results

Figure: Bioluminescent imaging of implanted macrophage–biopolymer mixes. IC-21-Luc macrophages (CC or BES) were mixed with biopolymer (Hydrogel, Soft, or Soft+) and subcutaneously injected into the dorsal fl anks of C57Bl/6 mice (three mice per hydrogel, with CC on the left fl ank and BES on the right). Following intraperitoneal injection of delta-luciferin, macrophage bioluminescence was detected using an IVIS Lumina II imaging system. A representative image from day 1 post-implantation (and 25 min post luciferin injection) is shown, indicating the peak detectable radiance (photons s −1 cm −2) in identically sized regions of interest. The CE results were very similar to the BES implants.

Figure:  Histological analysis of the macrophage–biopolymer implantation site (MSB staining). At day 4 post-implantation, skin was harvested from each dorsal fl ank, and processed for histology. Where possible, serial sections directly adjacent to those shown in Figure 4 were stained with a modifi ed trichrome stain. Mature fi brillar collagen within the dermis appeared dark blue, while the collagen within the hydrogel was generally lighter blue in appearance, indicating a less highly cross-linked or fi brillar form of collagen. Scale bar: 200 µm.

These Collagen Hydrogels are proving easy to use and effective in the hands of our customers. If you are looking for solutions for tissue reconstruction/repair or 3-D in vivo like cell based assays, do not hesitate to contact me @ direct phone: 612-801-1007 or pshuster@neuromics.com. Thank you, Pete Shuster, CEO and Owner, Neuromics.

Saturday, November 07, 2015

Human alpha-Sensory Neurons

Put Them to Work!

We continue to add unique primary Human Neurons to our offerings.

We have discounted our new alpha-Sensory Neurons to make it easier to buy and try-only 850 USD/1,000,000 cells.

Just thaw and culture. Simply culture on ECM- Laminin or Fibronectin coatings with our alpha Motor Neuron Maintaining Media. Questions? Please contact me directly 612-801-1007 or pshuster@neuromics.com. Pete Shuster, CEO and Owner, Neuromics.

Thursday, October 29, 2015

Solutions for Blood Brain Barrier Research

Neuromics Has the Key Components!

We are pleased to add Human Brain Microvascular Pericytes (HBMVPCs) to our solution set for Blood Brain Barrier (BBB) Researchers. These join our Human Brain Microvascular Endothelial Cells and hAstroPro Human Astrocyte+Astroglial-Neurons Co-Culturing Kits to round out our offerings of cells involved in BBB regulation.
Pericytes function at the BBB in at least two ways: by regulating BBB-specific gene expression patterns in endothelial cells, and by inducing polarization of astrocyte end-feet surrounding CNS blood vessels (see: http://www.nature.com/nature/journal/v468/n7323/full/nature09522.html).

Questions? Do not hesitate to call me or e-mail me-612-801-1007 or pshuster@neuromics.com). Pete Shuster CEO and Owner.

Tuesday, October 20, 2015

Brain Freeze!

Our Friend are Taking Neuromics Brain on Interesting #brainadventure

It is getting harder to select the winners. You can submit your entry now and earn a chance to win our monthly $100 monthly and $500 grand prize. To enter see: http://www.neuromics.com/brain-adventure-contest.

We can all relate to this.

Tuesday, October 13, 2015

hNP1 Neural Progenitors and Neuronal Regeneration after Injury

The Potential of Axonal PPARγ

In this study, researchers used our hNP1TM Neural Progenitors to demonstrate that axonal PPARγ is involved in neuronal injury responses required for axonal regeneration: Juan Pablo Lezana, Shachar Y. Dagan, Ari Robinson, Ron S. Goldstein, Mike Fainzilber, Francisca C. Bronfman, and Miguel Bronfman. Axonal pparγ promotes neuronal regeneration after injury. DOI: 10.1002/dneu.22353... Culture and regeneration analysis of human axons. NP1 human neural precursors were purchased from Neuromics (Minnesota, USA) and passaged up to 8 times before generation of neurons. Cells were used up until passage 10 after defrosting the purchased neural...

It is important to note that the researchers built up a large stock by passaging the progenitors prior to differentiation.

Check out our hNP1 publications to learn of more unique applications!

Thursday, October 01, 2015

#brainadventure Update

2 Win $100 Monthly Prices!

Our customers are creative. They find interesting #brainadventures for our Neuromics' Brains. Competitive hing for winning our $100 monthly and $500 grand prizes: more entries raise your odds of winning.

Here're some of the submissions:

Learn how you can win @ http://www.neuromics.com/brain-adventure-contest.

Good luick to all.

Saturday, September 26, 2015

Retinal Microvascular Endothelial Cells & Diabetic Retinopathy

HRMECS in Action

Our Human Retinal Microvascular Endothelial Cells in this publication on Diabetic Retinopathy. In this study, the authors show that human retinal microvascular endothelial cells exposed to high levels of glucose regulate miR-200b repression through histone methylation and that inhibition of PRC2 increases miR-200b while reducing VEGF: Michael Anthony Ruiz, Biao Feng, and Subrata Chakrabarti. Polycomb Repressive Complex 2 Regulates MiR-200b in Retinal Endothelial Cells: Potential Relevance in Diabetic Retinopathy. PLoS One. 2015; 10(4): e0123987. Published online 2015 Apr 17. doi: 10.1371/journal.pone.0123987...To investigate PRC2 and miR-200b regulation in the context of diabetic retinopathy, the major cell type used for investigation was the human retinal microvascular endothelial cell (HRMECs,Neuromics/ Olaf Pharmaceuticals, Worchester, MA, Cat# HEC09)...

Figure: High levels of glucose alter VEGF and miR-200b expression in HRMECs. A: HRMECs exposed to various concentrations of D-glucose for 24 hours exhibited differential mRNA levels of VEGF. Compared to 5mM D-glucose, VEGF expression was significantly increased at 15mM and 25mM D-glucose concentrations, with no change at 20mM L-glucose. B: Measured by WST-1 assay, HRMECs exposed to increasing concentrations of D-glucose for 24 hours exhibited decreased cell viability at 25mM, 50mM and 100mM compared to 5mM. C: HRMECs exposed to 25mM (high glucose; HG) glucose for 24 and 48 hours demonstrated significantly increased VEGF mRNA compared to 5mM (normal glucose; NG). These differences were not observed at time points earlier than 24 hours. D,E: HRMECs exposed to 5mM D-glucose (NG) 25mM D-glucose (HG) and 20mM L-glucose+5mM D-glucose (osmotic control; OSM). HRMECs cultured for 24 hours and 48 hours in HG showed significantly decreased levels of miR-200b with parallel increased levels of VEGF expression compared to NG and OSM. F,G: VEGF is also increased at the protein level in HG compared to NG as measured by Western Blotting. [* p < 0.05 compared to NG; n = 6; data expressed as mean ± SEM, normalized to β-actin or U6 and expressed as a fold change of NG]. doi:10.1371/journal.pone.0123987.

Check out our growing catalog of Human Endothelial Cells-Artery, Microvascular, Vein, Umbilical Cord Derived + Culture Media.

Thursday, September 17, 2015

Mouse and Human Motor Neurons

Designed for Neuro-muscular Diseases Research

Clients have been using our easy to culture and research proven GFP Labeled Mouse Motor Neurons for Neuro-muscular disease research. This includes the inclusion of the cells in several ALS drug discovery programs being conducted by large Pharma.

Image: GFP+ mMN Mouse Motor Neurons at 2 days post thaw 20X.

I am pleased to announce the addition of Human Motor Neurons to our Primary Human, Mouse and Rat Neurons, Astrocytes and Neuron-Astroglial co-culture solutions.

Image: Human alpha-Motor Neurons

Questions?  Do not hesitate to contact me directly, Pete Shuster, CEO and Owner, Neuromics, pshuster@neuromics.com and direct phone: 612-801-1007. Thank you.

Friday, September 11, 2015

hNP1 Neural Progenitors and HTS Tox Assays

Sensitivity of Neural Progenitor Cells to Chemical Induced Apoptosis

This article demonstrates the sensitivity of Neuromics' hNP1TM Neural Progenitors to chemically induced apoptosis: Ingrid Druwea, Theresa M. Freudenrich, Kathleen Wallace, Timothy J. Shafer, William R. Mundy. Sensitivity of neuroprogenitor cells to chemical-induced apoptosis using a multiplexed assay suitable for high-throughput screening. doi:10.1016/j.tox.2015.03.011.

Summary: The results demonstrate that, (1) all three commercially available models generated a robust source of proliferating neuroprogenitor cells, and that the assay was sensitive and reproducible when used in a multi-well plate format; (2) there were differences in the response of the rodent and human neuroprogenitor cells to a set of chemicals previously shown to induce apoptosis in vitro; and (3) proliferating neuroprogenitor cells were more sensitive to chemical-induced apoptosis than differentiated neurons, suggesting that neuroprogenitor cells are one of the cell models that should be considered for use in a developmental neurotoxicity screening battery.

Here're more publications of referencing use of hNP1 Neural Progenitors:
Yuji Kaneko, Hideki Shojo, Jack Burns, Meaghan Staples, Naoki Tajiri, Cesar V. Borlongan, DJ-1 ameliorates ischemic cell death in vitro possibly via mitochondrial pathway, Neurobiology of Disease, Available online 21 September 2013, ISSN 0969-9961, http://dx.doi.org/10.1016/j.nbd.2013.09.007...Cell culture and oxygen-glucose deprivation (OGD) hNPCs were obtained from Neuromics...

Xiufang Guo, Severo Spradling, Maria Stancescu, Stephen Lambert, James J. Hickman. Derivation of sensory neurons and neural crest stem cells from human neural progenitor hNP1. Biomaterials, In Press, Corrected Proof,Mar 2013.doi:10.1016/j.biomaterials.2013.02.061 ...hNP1, were obtained from Neuromics (Edina, Minnesota)...

Questions? Do not hesitate to contact me, Pete Shuster: pshuster@neuromics.com or direct phone: 612-801-1007.

Thursday, September 03, 2015

Connective-Tissue Growth Factor (CTGF/CCN2) Induces Astrogenesis

SOX-2 Proves a Marker For Astrogenesis

This proved  surprising to the authors of a recent publication referencing use of our Monoclonal SOX-2 Antibody. We have added our SOX-2 abs to our Glial-Astrocyte Markers. See: Fabio A. Mendes , Juliana M. Coelho Aguiar , Suzana A. Kahn, Alice H. Reis, Luiz Gustavo Dubois, Luciana Ferreira Romão, Lais S. S. Ferreira, Hervé Chneiweiss, Vivaldo Moura Neto, José G. Abreu. Connective-Tissue Growth Factor (CTGF/CCN2) Induces Astrogenesis and Fibronectin Expression of Embryonic Neural Cells In Vitro. Published: August 4, 2015DOI: 10.1371/journal.pone.0133689...monoclonal anti-Sox2 (Neuromics, Acris, Germany) in blocking solution were incubated with the membranes overnight at 4°C, followed by incubation with Peroxidase-conjugated anti-rabbit IgG and peroxidase-conjugated anti-mouse IgG secondary antibodies...

Figure: Exogenous CTGF protein increases the number of Sox2-positive cells of a neural progenitor culture. Immunostaining showing Sox2 (A and D) expression of untreated and CTGF-treated cells. C and F show merged pictures of Sox2 immunostaining together with nuclei-DAPI staining. Scale bars 10 μm. G shows the percentage of cells that were positive for Sox2. doi:10.1371/journal.pone.0133689.g002

Neuromics have a significant catalog of potent and proven Asytoglial, Neuronal, Schwann Cell and Oligodendrocyte Markers.

Friday, August 28, 2015

Small Molecules/Peptides for Pain Researchers

Agonists, Antagonists, Inhibitors and Ligands

The roots of Neuromics is providing solutions for the study of nociceptive and neuropathic pain. Our tools are widely used and frequently published. We are pleased to bring you these small molecules designed to help you better understand the roots of pain and potential therapies.

Figure: Infarct volume after 3 days of reperfusion in the ipsilateral cortex and CP complex in male rats treated with vehicle saline (n=15) or 1 mg/kg per hour BRL 52537 (n=15) started at onset of reperfusion and continued for 22 hours (male-vehicle n=15; male-BRL n=15; mean±SEM). P<.0.05. Related Pub.

Catalog #
10 mg
50 mg
10 mg
50 mg
10 mg
50 mg
10 mg
10 mg
50 mg
10 mg
50 mg
10 mg
1 mg
1 mg
2 mg
5 mg
5 mg
10 mg
10 mg
10 mg
10 mg
1 mg
1 mg

Questions? Do not hesitate to contact me @ pshuster@neuromics.com or 612-801-1007. Thank you! Pete Shuster, CEO and Owner, Neuromics.

Wednesday, August 26, 2015

Take Your Neuromics' Brain on an Adventure

Grand Prize is 500 USD!

Have fun. We can't wait to see your ideas.

Friday, August 14, 2015

Have Brain; Will Travel

Join the Adventure and You Could Win 500 USD
Contest begins September 2015. Monthly winners receive 100 USD. Contest will run for 6 months. Grand prize is 500 USD.
Join Neuromics on our‪#‎BrainAdventure‬ to see all the interesting and unique locations the brain ventures. Starting this September, with all orders, you will receive a Neuromics brain keychain. Take a creative picture or short video of the Neuromics Brain Keychain and tweet it @Neuromics, post it on Neuromics Facebook or LinkedIn Page and be entered in our contest. In the meantime, if you want to join the contest, e-mail rose@neuromics.com and she will mail you your brain.

Wednesday, August 12, 2015

Medical Marijuana Research

What we are learning.

I have reported here Medical Marijuana research and its therapeutic value for treating pain. The specifics posts feature publications that reference use of our Pain and Inflammation Research Markers. These include: J. Desroches, J.-F. Bouchard, L. Gendron, P. Beaulieu. Involvement of cannabinoid receptors in peripheral and spinal morphine analgesia ☆ Neuroscience, Volume 261, 7 March 2014, Pages 23–42.http://dx.doi.org/10.1016/j.neuroscience.2013.12.030.
Iryna A. Khasabova,Sergey Khasabov, Justin Paz, Catherine Harding-Rose, Donald A. Simone, and Virginia S. Seybold. Cannabinoid Type-1 Receptor Reduces Pain and Neurotoxicity Produced by Chemotherapy. The Journal of Neuroscience, 16 May 2012, 32(20): 7091-7101; doi: 10.1523/​JNEUROSCI.0403-12.2012

Here researchers study the impact of Marijuana on memory impairment using our one of our 5-HT Serotonin AntibodiesXavier Viñals, Estefanía Moreno, Laurence Lanfumey, Arnau Cordomí, Antoni Pastor, Rafael de La Torre, Paola Gasperini, Gemma Navarro, Lesley A. Howell, Leonardo Pardo, Carmen Lluís, Enric I. Canela, Peter J. McCormick, Rafael Maldonado, Patricia Robledo. Cognitive Impairment Induced by Delta9-tetrahydrocannabinol Occurs through Heteromers between Cannabinoid CB1 and Serotonin 5-HT2A Receptors. Published: July 9, 2015DOI: 10.1371/journal.pbio.1002194.
Impact on Memory: Activation of cannabinoid CB1 receptors (CB1R) by delta9-tetrahydrocannabinol (THC) produces a variety of negative effects with major consequences in cannabis users that constitute important drawbacks for the use of cannabinoids as therapeutic agents. For this reason, there is a tremendous medical interest in harnessing the beneficial effects of THC. Behavioral studies carried out in mice lacking 5-HT2A receptors (5-HT2AR) revealed a remarkable 5-HT2AR-dependent dissociation in the beneficial antinociceptive effects of THC and its detrimental amnesic properties We found that specific effects of THC such as memory deficits, anxiolytic-like effects, and social interaction are under the control of 5-HT2AR, but its acute hypolocomotor, hypothermic, anxiogenic, and antinociceptive effects are not. In biochemical studies, we show that CB1R and 5-HT2AR form heteromers that are expressed and functionally active in specific brain regions involved in memory impairment. Remarkably, our functional data shows that costimulation of both receptors by agonists reduces cell signaling, antagonist binding to one receptor blocks signaling of the interacting receptor, and heteromer formation leads to a switch in G-protein coupling for 5-HT2AR from Gq to Gi proteins. Synthetic peptides with the sequence of transmembrane helices 5 and 6 of CB1R, fused to a cell-penetrating peptide, were able to disrupt receptor heteromerization in vivo, leading to a selective abrogation of memory impairments caused by exposure to THC. These data reveal a novel molecular mechanism for the functional interaction between CB1R and 5-HT2AR mediating cognitive impairment. CB1R-5-HT2AR heteromers are thus good targets to dissociate the cognitive deficits induced by THC from its beneficial antinociceptive properties. 

Figures: 5-HT2AR and CB1R form heteromers in transfected cells. In (A), BRET saturation experiments were performed in HEK-293T cells transfected with 0.025 μg of 5-HT2AR-Rluc cDNA and increasing amounts of CB1R-YFP cDNA (0.05 μg to 1.5 μg, black curve), with 0.5 μg of dopamine D1R-Rluc cDNA and increasing amounts of CB1R-YFP cDNA (0.5 μg to 6 μg, yellow line), or with 0.025 μg of 5-HT2AR-Rluc cDNA and increasing amounts of adenosine A1R-YFP cDNA (0.05 μg to 1.5 μg, red line). The relative amount of BRET is given as a function of 100 x the ratio between the fluorescence of the acceptor (YFP) and the luciferase activity of the donor (Rluc). BRET is expressed as milli BRET units (mBU) and is given as the mean ± standard deviation (SD) of 3–6 experiments grouped as a function of the amount of BRET acceptor. In (B), a schematic representation of fluorescence complementation experiments is depicted in the left panel showing that fluorescence only appears after the YFP Venus hemiprotein complementation due to the proximity of two receptors fused to hemi-YFP Venus proteins (cYFP or nYFP). In the right panel, fluorescence at 530 nm was detected in HEK-293T cells transfected with different amounts of cDNA corresponding to both 5-HT2AR-cYFP and CB1R-nYFP (equal amount for each construct), but not in negative controls in which cells were transfected with cDNA corresponding to 5-HT2AR-cYFP and the noninteracting adenosine A1 receptor-nYFP or CB1R-nYFP and the noninteracting dopamine D1 receptor-cYFP. One-way ANOVA followed by a Dunnett’s multiple comparison test showed a significant fluorescence over basal values in HEK-293T cells (** p< 0.01, *** p< 0.001). In (C), PLAs were performed in HEK-293T cells expressing CB1R and 5-HT2AR. Confocal microscopy images (superimposed sections) are shown in which heteromers appear as green spots. In all cases, cell nuclei were stained with DAPI (blue). Scale bars = 20 μm. In (D), PLAs were performed in nontransfected HEK-293T cells, cells transiently transfected with 0.5 μg of CB1R or 5-HT2AR cDNA (negative controls, white columns), or with increasing amounts of CB1R and 5-HT2AR cDNA (black columns). In each case, the ratio between the number of green spots and the number of cells showing spots (ratio r) was calculated. One-way ANOVA followed by a Dunnett’s multiple comparison test showed a significant PLA staining over nontranfected cells (*** p <; 0.001). doi:10.1371/journal.pbio.1002194.g004

Yes, memory impairment must be considered as a side effect of Medical Marijuana, I am confident there are human subjects that would volunteer for cognition studies to get a clearer picture of the short and long term impact on users.