New Publication Referencing Neuromics' NSC Markers

Sujoy K. Dhara, Kowser Hasneen, David W. Machacek, Nolan L. Boyd, Raj R. Rao, Steven L. Stice (2008) Human neural progenitor cells derived from embryonic stem cells in feeder-free cultures doi:10.1111/j.1432-0436.2007.00256.x

...NES (1:100, Neuromics, Edina, MN), MSI1 (1:100, Neuromics), Tuj1 (1:500, Neuromics)...

Recent Pubs Referencing Neuromic's Products

Neurotensin Receptor 1 (NTS1)-GP14020

Geneviève Roussy, Marc-André Dansereau, Louis Doré-Savard, Karine Belleville, Nicolas Beaudet, Elliott Richelson and Philippe Sarret. Spinal NTS1 receptors regulate nociceptive signaling in a rat formalin tonic pain model. doi:10.1111/j.1471-4159.2007.05205.x

Images: Expression of NTS1 receptors in sensory neurons and spinal cord. (a) Immunoperoxidase staining reveals the presence of NTS1-like immunoreactivity throughout the lumbar dorsal horn of the spinal cord. Immunolabeling is most prominent in the superficial layers of the dorsal horn. The lateral spinal nucleus (lsn) is also moderately labeled (arrow). (b) High magnification of (a). A dense NTS1 immunostaining is observed over laminae I and II of the dorsal horn. Numerous immunopositive nerve cell bodies are visible in the superficial laminae and in the nucleus proprius of the dorsal horn (arrows). (c) Light microscopic analysis of NTS1 expression in primary afferent neurons. NTS1 is expressed in subpopulations of small- and medium-ganglion cells. No apparent labeling is detected in large DRG neurons (arrows). At higher magnification, NTS1 neurons exhibit a cytoplasmic pattern of immunoreactivity (d, arrowheads). (e) Identification of endogenously expressed NTS1 receptors, by western blotting, in homogenates from DRGs, lumbar spinal cord and brain. The 47 kDa protein band corresponds to the molecular weight deduced from the cDNA sequence of NTS1. Each lane represents the transfer of 25 lg of protein. Scale bars: (a, b, c, and d) 300, 150, 70 and 20 lm, respectively. doi:10.1111/j.1471-4159.2007.05205.x

Primary Neurons and Astrocytes

Cortex
Hippocampus
Striatum
E18 and E20 Rat Primary Neuronal Tissue -NEURON CULTURES
E18 Rat Primary Neuronal Tissue - ASTROCYTE CULTURES
E18 Mouse Neuronal Tissue -NEURON CULTURES
E18 Mouse Neuronal Tissue -ASTROCYTE CULTURES
Frozen Primary Rat Neurons-NEW
Frozen Primary Mouse Neurons and Astrocytes-New
FluoGreen Tracer-Stains Cell Nuclei and Cytoplasm

Primary Neurons and Astrocytes

Shouqing Luo, Haruo Mizuta and David C. Rubinsztein. p21-activated kinase 1 promotes soluble mutant huntingtin self-interaction and enhances toxicity. Human Molecular Genetics 2008 17(6):895-905; doi:10.1093/hmg/ddm362

...Rat E18 cortical and striatal neurons were purchased from Neuromics (MN) or prepared from E18 rat brain tissues according to instructions from Neuromics, cultured in neurobasal (Invitrogen) medium supplemented with B27 (Invitrogen) and 0.5 mM glutamine and transfected with Lipofectamine 2000 according to standard methods...

New Data and Pubs referencing Neuromics

Cullen L. Schmid, Kirsten M. Raehal and Laura M. Bohn. Agonist-directed signaling of the serotonin 2A receptor depends on ?;-arrestin-2 interactions in vivo. Published online on January 14, 2008, 10.1073/pnas.0708862105
...Neuromics, Edina, MN; rabbit anti-5HT ..... 5A-5D...


Thomas W. Gould, Shigenobu Yonemura, Ronald W. Oppenheim, Shiho Ohmori, and Hideki Enomoto. The Neurotrophic Effects of Glial Cell Line-Derived Neurotrophic Factor on Spinal Motoneurons Are Restricted to Fusimotor Subtypes. The Journal of Neuroscience, February 27, 2008, 28(9):2131! -2146
...mouse Ret (Neuromics, Edina, MN), human Ret (Neuromics), and activated caspase-3 (Cell Signaling, Danvers, MA). Muscles or sections were rinsed and then...


Image: Nuclei of D3 or IGnT-KO ES cells stained with goat anti-Oct3/4 antibody. doi: 10.1093/glycob/cwm138

New Nature Pub Referencing Neuromics' TIE1

Tobias Schatton, George F. Murphy, Natasha Y. Frank, Kazuhiro Yamaura, Ana Maria Waaga-Gasser, Martin Gasser, Qian Zhan, Stefan Jordan, Lyn M. Duncan, Carsten Weishaupt, Robert C. Fuhlbrigge, Thomas S. Kupper, Mohamed H. Sayegh, Markus H. Frank. Identification of cells initiating human melanomas. Nature 451, 345 - 349 (17 Jan 2008) Letter
...goat anti-human TIE1 Ab (Neuromics)...


Product Page Link:
TIE1-Catalog#: GT15147
TIE-2
Cancer Antibodies
Stem Cell Reagents

Nov-Dec Publication Referencing Neuromics

Diversity in the Neural Circuitry of Cold Sensing Revealed by Genetic Axonal Labeling of Transient Receptor Potential Melastatin 8 Neurons
CELLULAR/MOLECULAR:Yoshio Takashima, Richard L. Daniels, Wendy Knowlton, James Teng, Emily R. Liman, and David D. McKemyDiversity in the Neural Circuitry of Cold Sensing Revealed by Genetic Axonal Labeling of Transient Receptor Potential Melastatin 8 NeuronsJ. Neurosci., Dec 2007; 27: 14147 - 14157 ; doi:10.1523/JNEUROSCI.4578-07.2007 ......211; Santa Cruz Biotechnology, Santa Cruz, CA), 1:500 rabbit anti-transient receptor potential vanilloid 1 (TRPV1; RA14113; Neuromics, Edina, MN), and 1:500 rabbit anti-TRPM8 (a gift from M. Tominaga, Okazaki Institute for Integrative Bioscience, Okazaki......
Abstract
Full Text
PDF
Supplemental Data
G 5 Is Required for Normal Light Responses and Morphology of Retinal ON-Bipolar Cells
BRIEF COMMUNICATIONS:Anjali Rao, Rebecca Dallman, Scott Henderson, and Ching-Kang ChenGβ5 Is Required for Normal Light Responses and Morphology of Retinal ON-Bipolar CellsJ. Neurosci., Dec 2007; 27: 14199 - 14204 ; doi:10.1523/JNEUROSCI.4934-07.2007 ......2003) 1:100 RGS6 Imgenex (San Diego, CA) 1:100 Goalpha Millipore (Billerica, MA) 1:100 mGluR6 Neuromics (Northfield, MN) 1:100 PKCalpha Sigma 1:100 FLAG Sigma 1:100 Transmission electron microscopy......
Abstract
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Exogenous Delivery of Heat Shock Protein 70 Increases Lifespan in a Mouse Model of Amyotrophic Lateral Sclerosis
NEUROBIOLOGY OF DISEASE:David J. Gifondorwa, Mac B. Robinson, Crystal D. Hayes, Anna R. Taylor, David M. Prevette, Ronald W. Oppenheim, James Caress, and Carolanne E. MilliganExogenous Delivery of Heat Shock Protein 70 Increases Lifespan in a Mouse Model of Amyotrophic Lateral SclerosisJ. Neurosci., Nov 2007; 27: 13173 - 13180 ; doi:10.1523/JNEUROSCI.4057-07.2007 ......overnight, at 4C. The primary antibodies used for glial cell activation were glial fibrillary acidic protein (GFAP; RA22101; Neuromics, Minneapolis, MN) and ionized calcium binding adaptor molecule 1 (Iba1; 016-20001; Wako, Neuss, Germany). The primary antibody......
Abstract
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Modulation of Semaphorin3A Activity by p75 Neurotrophin Receptor Influences Peripheral Axon Patterning
DEVELOPMENT/PLASTICITY/REPAIR:Ayal Ben-Zvi, Liat Ben-Gigi, Hagit Klein, and Oded BeharModulation of Semaphorin3A Activity by p75 Neurotrophin Receptor Influences Peripheral Axon PatterningJ. Neurosci., Nov 2007; 27: 13000 - 13011 ; doi:10.1523/JNEUROSCI.3373-07.2007 ......domain) used in Western blot analyses was purchased from Alamone Labs (Jerusalem, Israel). Anti-Neuropilin1 was purchased from Neuromics (Edina, MN). Secondary antibodies were obtained from Jackson Immunoresearch Laboratories (Jackson, PA). Protease inhibitor......
Abstract
Full Text
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Supplemental Data
Somatostatin Peptides Produce Multiple Effects on Gating Properties of Native Cone Photoreceptor cGMP-Gated Channels That Depend on Circadian Phase and Previous Illumination
CELLULAR/MOLECULAR:Shih-Kuo Chen, Gladys Y.-P. Ko, and Stuart E. DryerSomatostatin Peptides Produce Multiple Effects on Gating Properties of Native Cone Photoreceptor cGMP-Gated Channels That Depend on Circadian Phase and Previous IlluminationJ. Neurosci., Nov 2007; 27: 12168 - 12175 ; doi:10.1523/JNEUROSCI.3541-07.2007 ......into the plasma membrane, and this can be monitored to infer activation of PLC. Transfection was performed using pn-Fect from Neuromics (Edina, MN). Briefly, retinal cells were grown for 4 d in LD cycles, until 24 h before analysis. At that time, coverslips......

Brainblow

'Brainbow' paints mice neurons in bright colors
Powerful tool could help map out the brain's complicated wiring plan




By Julie Steenhuysen

Reuters

updated 1:24 p.m. CT, Wed., Oct. 31, 2007


CHICAGO - U.S. researchers have manipulated neurons in mice to light up in a rainbow of fluorescent hues, creating a powerful tool for mapping out the brain's complicated wiring plan, they said on Wednesday.
This "Brainbow," as they call it, will allow researchers to trace neurons throughout the nervous system, gaining new understanding of its organization and function.
"It has been exceedingly hard to figure out these wiring diagrams," said Joshua Sanes, professor in the Department of Molecular and Cellular Biology and Center for Brain Science at Harvard University. "That could be a main use of this."
A portion of the hippocampus from a 'Brainbow' transgenic mouse is shown in this undated handout image. U.S. researchers have manipulated neurons in mice to light up in a rainbow of fluorescent hues, creating a powerful tool for mapping out the brain's complicated wiring plan.

View related photos

Striatal Neurons Image

Thank you to Dr. Henry Guo, Intracellular Therapies

Larger Striatal Image (10X)

E18 and E20 Rat Primary Neuronal Tissue -NEURON CULTURES
Fresh Tissue and media are provided to initiate a culture of primary neurons.
E18 Rat Primary Neuronal Tissue - ASTROCYTE CULTURES
Fresh Tissue and media are provided to initiate a culture of primary astrocytes.
Human Brain Tissue (Blocks, Lysates and Slides)-New
Healthy adult human brain tissue
Frozen Primary Rat Neurons-NEW
From E18 Rat Cortex
Frozen Primary Mouse Neurons-New
Growth Media and Poly-D-Lysine Coverslips

SfN Presentation-i-Fect and siRNA in vivo

We are excited about this upcoming presentation at Society for Neuroscience comference presesentation. Neuromics' i-Fect ™ reagent was used to deliver siRNA in vivo to silence the NTS2 gene.

Program#/Poster#:509.6/PP9
Title:Small interfering RNA-mediated selective knockdown of NTS2 receptors reverses neurotensin-induced analgesia in rats
Location:San Diego Convention Center: Halls B-H
Presentation Start/End Time:Monday, Nov 05, 2007, 2:00 PM - 3:00 PM
Authors:*L. DORE-SAVARD1,2, G. ROUSSY1, M.-A. DANSEREAU1, K. BELLEVILLE1, N. BEAUDET1, M. BEHLKE2, P. SARRET1; 1
Physiology and Biophysics, Univ. Sherbrooke, Sherbrooke, PQ, Canada; 2Integrated DNA Technologies Inc., Coralville, IA

2007 Copyright by the Society for Neuroscience all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.

Musashi Customer Data

Musashi-1

Image: Musashi-positive cells in the ventricular zone of E14.5 mouse medulla. Picture was taken at 200x magnification. The sections are 4% PFA fixed, paraffin-embedded and cut at 5 micron. Courtesy of Xi Huang, Chiang Lab, VUMC

Hot off the Presses

Blackwell Synergy - Headache, Volume 47 Issue 7 Page 1008-1023 ...07 Jun 2007 Issue online: 12 Jul 2007. Accepted for publication April 19, .... Neuromics, Edina, MN, USA), active p38 MAP kinase (1:200; Cell Signaling ...
www.blackwell-synergy.com/doi/abs/10.1111/j.1526-4610.2007.00854.x - Similar pages

New GF Proteins

We are pleased to inform you of new Growth Factor Proteins. These reagents are well characterized, research proven and data rich. Even better, they are priced at a level that makes the intial buy decision easy. These are the initial proteins we have added to our catalog. We will be adding 30-50 per month and keep you updated.

BMP-7 CHO
CNTF
EGF
EGF
EGF Pichia
EGF-21 Leu
FGF-1, acidic
FGF-1, acidic, Sf9
FGF-19
FGF-2
FGF-2, basic
FGF-2, basic, Sf9
FGF-21
FGF-22
FGF-9
FGF-9
GDNF
NGF-b
NGF-b CHO
ProNGF

Image: GDNF Structure and Sequence

Nesting Staining of Grafted Human Neural Progenitor Cells

We would like to thank Dr. Rike Zietlow of Cardiff University for generously providing us this image.

Image: Nestin (Catalog# GT15012) staining of human neural progenitor cells grafted into the rat brain, 24 weeks post-op. One is of processes running along the host corpus callosum, one of cells in the hippocampus, weakly counterstained with human nuclear antigen (brown). Nestin concentration was 1:1000, overnight at room temp, visualised using the DAKO vector SG kit.

APJ (Apelin Receptor) in the Gut

Great Image of our APJ-Catalog#:MO15011

Image: Confocal microscopy of rabbit gastric gland distinguishing the distribution of HDC and the apelin receptor. Green pseudocolor: FITC-coupled polyclonal anti-HDC ab. Red pseudocolor: TRITC-coupled monoclonal anti-APJ receptor ab. Two cells in this gland show specific anti-AJP receptor and anti-HDC staining. Right: a higher magnification scan of the cell in the top right corner of the left panel showing peripheral APJ receptor localization and central perinuclear HDC staining. Physiol. Genomics 25: 153-165, 2006

APJ (Apelin Receptor) in the Gut

Great Image of our APJ-Catalog#:MO15011

Image: Confocal microscopy of rabbit gastric gland distinguishing the distribution of HDC and the apelin receptor. Green pseudocolor: FITC-coupled polyclonal anti-HDC ab. Red pseudocolor: TRITC-coupled monoclonal anti-APJ receptor ab. Two cells in this gland show specific anti-AJP receptor and anti-HDC staining. Right: a higher magnification scan of the cell in the top right corner of the left panel showing peripheral APJ receptor localization and central perinuclear HDC staining. Physiol. Genomics 25: 153-165, 2006

Ret in Recent Pub

Our Ret-Catolog#: GT15002 is referenced in the publication:
Bhupinder P.S. Vohra , William Planer , Jennifer Armon , Ming Fu , Sanjay Jain , Robert O. Heuckeroth Reduced endothelin converting enzyme-1 and endothelin-3 mRNA in the developing bowel of male mice may increase expressivity and penetrance of Hirschsprung disease-like distal intestinal aganglionosis
Developmental Dynamics 236:106–117, 2007... goat anti-Ret antibody (Neuromics,. Inc., Minneapolis, MN, 1:200)...























Figure 2. Testosterone and Mullerian inhibiting substance (MIS) do not have deleterious effects on enteric nervous system (ENS) precursor proliferation, differentiation, or survival. A-D: Cultured immunoselected embryonic day (E) 12.5 C57BL/6 ENS precursors were grown for 24 hr with or without glial cell line-derived neurotrophic factor (GDNF), testosterone, or MIS. A-C: Triple-label immunohistochemistry was performed with antibodies to Ret (A), neuron-specific beta III tubulin (TuJ1; B), or histone 3 phosphate (H3P; C). D: Shows the merged image. Arrowheads show a Ret+ TuJ1+ H3P- cell. Arrows identify a cell with a punctate nuclear staining pattern characteristic of H3P immunohistochemistry. Both TuJ1 and H3P antibodies were generated in rabbits, but easily were distinguished based on characteristic staining patterns. E,H: Quantitative analysis of TuJ1 immunoreactivity in Ret+ cells shows that treatment with testosterone or MIS does not alter expression of the neuronal differentiation marker recognized by TuJ1 in the presence or absence of GDNF. F,I: Quantitative analysis of proliferation (H3P+ cells) shows that neither testosterone nor MIS influences proliferation of Ret+TuJ1+ cells in the presence or absence of GDNF. G,J: Quantitative analysis of proliferating Ret+TuJ1- cell demonstrates increased proliferation in response to testosterone, but not MIS in the absence of GDNF. There was no additive effect of testosterone plus GDNF under these conditions. Asterisks indicate P < n =" 3" bar =" 50">

Customer Data on New Alpha 2c

Alpha 2c staining of rat ventral spinal cord (dilution 1:100). para-picric fixative. Please note perinuclear labeling. This appears around most neurons in spinal cord.

Thank you to Dr. Patrick Carr, UND

GDF-3 and Cancer, Diabetes

We have just added a new GDF-3 to our BMPs/GDFs antibodies category.

Image: GDF Staining of mouse thymus. Tissue was stained with goat HRP-DAB (brown) and counterstained with Hematoxylin (blue

Description: The TGFp superfamily of ligands plays key functions in development and disease. In both human and mouse embryonic stem cells, a member of this family, GDF3, is specifically expressed in the pluripotent state. We show thatGDF3 is an inhibitor of its own subfamily, blocks classic BMP signaling in multiple contexts, interacts with BMP proteins and is expressed specifically in the node during gastrulation in a pattern consistent with BMP inhibition. Furthermore, we use gain- and reduction-of-function to show that in a species-specific manner, GDF3 regulates both of the two major characteristics of embryonic stem cells: the ability to maintain the undifferentiated state and the ability to differentiate into the full spectrum of cell.

GDF-3 is suspected to be expressed in certain forms of cancer. These could include Breast and Testicular Cancer (Link).

mGluR1 Alpha, Alpha 2c and TRPV1 Westerns

mGluR1 alpha (Catalog#: RA19065)

Species: Human, Mouse an Rat

Applications: ICC; IHC and WB

Immunogen: 1159-1171 SSVPSSPVSESVL GAEGGAGGADGQGAGP

VR1 (TRPV1) (Catalog#: GT15129)

Species: Rat

Applications: IHC and WB

KLH-coupled synthetic peptide corresponding to amino acid residues 4 - 21 of Vanilloid Receptor 1

2c Adrenergic Receptor (Catalog#: RA19064)

Species: Human and Rat

Applications: ICC; IHC and WB

Immunogen: 309-324

Customer Data-GFAP IHC

We would like to thank Dr. Qi-Lin Cao, University of Louisville Medical School for this excellent image.

Image: GFAP (Catalog#: CH22102) Staining of of Astrocytes in the Ventral horn and Funiculus of Adult Rat Spinal Cord.

Dilution 1:500

NP Y2 Image and Publication

We would like to thank Dr. Victor Nadler and his team for sharing this image and publication with us!
















NPY Y2 Receptor-Catalog#: RA14112 immunoreactivity in the rat hippocampus.

Related Publication:
B Tu, Y Jiao, H Herzog, JV Nadler. Neuropeptide Y regulates recurrent mossy fiber synaptic transmission less effectively in mice than in rats: correlation with Y2 receptor plasticity. Neuroscience, 143, 1085-1094 (2006)

P2Y2-It is baaack!

We have finally managed to make a new P2Y2 (Catalog#: RA14102) that passed our IHC certification process.




Image: P2Y2 Staining of Rat DRG.

Related Antibodies to Consider:
P2X1
P2X2-Guinea Pig
P2X2-Rabbit
P2X3-Rabbit
P2X3-Guinea Pig
Neuropeptides
Opioids
Opioid Neuropeptides
TRPV1s
Vision and Retina
Cancer

Excellent TUJ-1 IHC

We would like to thank Christine Graham and Doug Vetter, Tufts Univ. School of Medicine, Boston for the excellent image.



Tuj-1 Catalog#: MO15013 staining of
staining of mouse cochlea (whole mount, ~50um).

Tuj-1 Rabbit Polyclonal
All Neuron-Glial Markers
All Stem Cell Reagents

P2X3 Western Blot

There have recently been customer question on the expected band for our P2X3 Antibody (Catalog#: RA10109)
Here's an excellent publication showing both WB and IHC for this antibody.

P2X2 and P2X3 receptor expression in postnatal and adult rat urinary bladder and lumbosacral spinal cord Simon Studeny, Ali Torabi and Margaret A. Vizzard.




Figure: Western blot of whole urinary bladder (40 mg) for P2X3 receptor expression in postnatal (P0-P36) and adult (A) rats. Erk staining was used as a loading control. B. Preabsorption of P2X3 antibody with immunogen (1 mg ml−1) eliminated the band at 75kD. C. Histogram of relative P2X3 band density in all groups examined normalized to erk-1 staining. P2X3 receptor expression in urinary bladder is significantly increased from P14-P21 compared to adult rat bladder. *, p ≤ 0.005, #, p ≤ 0.01.

ESC to Neural Progenitors-Role of MOR-1C

Features use of Neuromics' MOR-1C

Eunhae Kim, Amy L. Clark , Alexi Kiss, Jason W. Hahn, Robin Wesselschmidt. MU AND KAPPA OPIOIDS INDUCE THE DIFFERENTIATION OF EMBRYONIC STEM CELLS TO NEURAL PROGENITORS
JBC Papers in Press. Published on September 1, 2006 as Manuscript M603862200






Image: A-D-Staining of Differentiated Stem Cells. D3 ES cells were maintained in serum/LIF/ME induced self-renewal conditions (undifferentiated cells, D3). Upon treatment with 1 µM RA they undergo differentiation (dD3). Cells were fixed and permeabilized. Treatments were followed by overnight incubation with polyclonal MOR-1 (C-terminus, 1:2500)

New TRPV1-C Publication

Amol M. Patwardhan, Nathaniel A. Jeske, Theodore J. Price, Nikita Gamper, Armen N. Akopian, and Kenneth M. Hargreaves. The cannabinoid WIN 55,212-2 inhibits transient receptor potential vanilloid 1 (TRPV1) and evokes peripheral antihyperalgesia via calcineurin. PNAS 103 (July 2006): 11393-11398.


















Figure: WIN activates calcineurin in TG neurons and dephosphorylates TRPV1. (A) Calcineurin subunits are coexpressed with TRPV1. The colocalization of calcineurin A subunit (Upper) and B subunit (Lower) with TRPV1isdemonstratedin the respective panels.(B) The effect of various treatments on the nuclear translocation of NFATc4 (calcineurin activation). Cultured TG neuronswere exposed to vehicle, ionomycin (1 M), capsaicin (1 M), WIN (25 M), or WINCAIP(25 M50 M), and immunohistochemistry was performed by using an antibody againstNFATc4. (C) Graphical representation of the percent of NFATc4 positive neurons showing nuclear translocation of NFAFc4 after treatment with VEHVEH, VEHWIN (25 M), CAIP (50 M)WIN, VEHCAP (1 M), or CAIP(50 M)CAP (n 4 independent cultures assessed by blinded observer; n 152–180 cells per condition; **, P<0.01,anovawith n="3">

4 New Interleukins

These Interleukins have been tested for IHC and WB apps and recommended for use by Diabetes/Obesity, Cancer, Neurodegenerative Disease and Pain/Inflammation Researchers

IL-1Alpha/IL-F1







Image: IL-1α staining of blood lymphocytes. Cells were stained using anti-gat-Cy3 (red) and counterstained with Fluoro Nissl Green







IL-1Beta






Image: IL-1 beta staining (red color) in mouse splenocytes stimulated with Con A. Cell nuclei counterstained green.






IL-1 R1



Image: IL-1R staining of mouse thymus. Staining was done using HRP-DAB (brown) detection. Tissue was counterstained with haematoxylin (blue).





IL-6





Image: IL-6 staining of mouse T cells. Cells were stained with goat anti-rat IL-6 (red) and nuclei counterstained with Fluoro Nissl Green.

Monoclonal Ubiquitin Antibodies-Great IHC

Ubiquitin is a protein that plays a role in the targeting of proteins for proteolytic degradation. Ubiquitin becomes covalently bonded to many types of pathological inclusions seen in neurodegenerative diseases that appear to be resistant to normal degradation. The neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, the Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can all be readily visualized with this antibody.


Ubiquitin-Catolog#:19005














Image: ABC-HRP staining with Ubiquitin monoclonal antibody (diluted 1:1,000) of Alzheimer’s disease cerebral cortex showing dystrophic neuritis, which contain ubiquitinated tau. The inclusions are detected by the Ubiquitin antibody. Normal cortex does not contain inclusions and would be white in color.

Ubiquitin-Catalog#: MO18001















Confocal images of hippocampal CA1 sections from rats, (A) mock-treated or (B) subjected to 15 minutes ischemia followed by 24 hours of reperfusion, using ubiquitin mouse antibody (green) and propidium iodide (red). Data courtesy of Cell Signaling Technology, Inc.

New ChAT IHC Data

A big thank you to the Patrick Carr Lab at University of North Dakota for the excellent image.

ChAT (Choline Acetyltransferase-Catalog#: CH23000)







Image: IHC Staining in Rat Brainstem








Other Reagents to Consider:
Neuron-Glial Markers-Chickens:
CNPase (CH23013)
GAP 43 (CH23004)
GFAP (CH23001)
GFAP (CH22102)
MAP2 (CH22103)
NSE (CH23003)
Nestin (CH23001)
Netrin-1 (CH23002)
NF-H (CH22104)
NF-L (CH22105)
NF-M (CH22106)
NF-M (CH23104)
PLP (CH23008)
Po (CH23009)
S-100 (CH23012)
Tuj 1 (CH23005)
All Neuron-Glial Markers
Stem Cell Reagents

New P2X3 Data

P2X3 Catalog#: RA10109

Data Courtesy of Dr. Yan Sun University of Maryland School of Medicine Baltimore
















Image: Double immunofluorescence staining in cultured human bladder urothelial cells (BUCs). BUCs show cytokeratin (red areas) and P2X3 (green areas) expression.

Figure: Flow cytometry and FACS analysis of P2X3 expression in human BUCs. P2X3 expression in BUCs were compared among in vitro stretched samples and unstretched samples in both normal and interstitial cystitis (IC) patients. A, unstretched normal BUCs. B, stretched normal BUCs. C, unstretched IC BUCs. D, stretched IC BUCs. M1 region represents P2X3 localization detected.