Tuesday, August 23, 2016

Human Mesenchymal Stem Cells Conjugates

Targeted Delivery of Stem Cells
Here's a recent publication referencing use of our Human Mesenchymal Stem Cells to form conjugates that could have application for drug delivery and cell therapies: Yun Seop Kim, Won Ho Kong, Hyemin Kim, Sei Kwang Hahn. Targeted Systemic Mesenchymal Stem Cell Delivery Using Hyaluronate - Wheat Germ Agglutinin Conjugate. http://dx.doi.org/10.1016/j.biomaterials.2016.08.027


Images: Fluorescence microscopic images of dissected livers and lungs from normal rats 4 h after intravenous injection of PBS, WGA-FITC/hMSC, and HA-WGA-FITC/hMSC (Filter for FITC). (b) Quantification of hMSCs in dissected organs estimated from ROI (n = 3). **P versus the WGA/hMSC group. (c) Representative fluorescence microscopic images of WGA-FITC/hMSC and HA-WGA-FITC/hMSC in the liver and lung (green = FITC, blue = DAPI for nucleus). Arrows indicate the location of hMSCs (scale bar = 200 μm). http://dx.doi.org/10.1016/j.biomaterials.2016.08.027
Protocol: Cultured MSCs were trypsinized, washed, and resuspended at 1 × 106 cells/mL in PBS. HA-WGA conjugate at 10 μg/mL of WGA was added to MSCs in suspension, and incubated in an ice bath for 10 min with mild mixing. To remove the unbound HA-WGA conjugate to MSCs, cell suspension was washed with PBS and collected by centrifugation (1000 × g, 3 min at 4 °C). After surface modification with HA-WGA conjugate, HA-WGA/hMSC were resuspended in the cell culture medium on 96-well plates (1 × 104 cells per well). At the predetermined time, cell viability of HA-WGA/hMSC was measured using an EZ-cytox cell viability assay kit according to the manufacturer’s instructions. Zeta potentials were analyzed using a Zetasizer Nano (Malvern Instruments, UK) to assess the change of surface charge after the surface modification.
Conclusions: Researchers successfully developed a target-specific systemic delivery system of MSCs to the liver using HA-WGA conjugate. HA-WGA conjugate was synthesized by the coupling reaction of HA-aldehyde with amine group of WGA. GPC analysis revealed the successful synthesis of HA-WGA conjugate. CD analysis corroborated that the secondary structure of WGA remained stable even after conjugation to HA. HA-WGA conjugate appeared to bind to the surface of MSCs and remain stably for up to 1 h. After cell surface modification, most of HA-WGA/MSC complex could be systemically delivered to the liver 4 h after intravenous injection, whereas MSCs were trapped mainly in the lung. This new strategy to target-specifically deliver MSCs to the liver using HA-WGA conjugate might be successfully exploited for treatment of various liver diseases.

We will continue to post new applications developed by users of our Stem Cells.

Friday, August 19, 2016

More on Epigenetics and Pain

Great Research Tools!

Our Markers and i-FectTM  Transection Kit are  used to study Epigenetics and pain. Here's yet another example: M. Leinders, b, N. Üçeyler, R.A. Pritchard, C. Sommer, L.S. Sorkin. Increased miR-132-3p expression is associated with chronic neuropathic pain. Experimental Neurology. Volume 283, Part A, September 2016, Pages 276–286...The inhibitor and mimetic were administered to awake rats via the it catheters. Prior to injection, active or mismatch inhibitors were mixed with (1:5 w/v) i-Fect™ in vivo transfection reagent(Neuromics, Edina, USA) to final doses of 5, 2 and 1 μg in 10 μl...

Figure: Inhibition of MOR. Stained with Dorsal Horn Neuromics' Rabbit Polyclonal Antbody.
Spinal administration of miR-132-3p antagonists via intrathecal (i.t.) catheters dose dependently reversed mechanical allodyina  and eliminated pain behavior in the place escape avoidance paradigm (p < 0.001). Intrathecal administration of miR-132-3p mimetic dose-dependently induced pain behavior in naïve rats (p < 0.001). Taken together these results indicate a pro-nociceptive effect of miR-132-3p in chronic neuropathic pain.

Finding like these could pave the way for an miRNA like therapy for pain.

Saturday, August 13, 2016

Protein Purification Solutions

Strep-tag®Tools + Strep-Tactin Columns

We are seeing a notable uptick in use of our Protein Purification Tools. This includes vectors and purification columns. We are especially delighted with the increased use of of our Strep-Tag + Strep-Tactin Columns.
Figure: Strep-Tag+Strep-Tactin Methods
Here's an example of researchers using the technology to purify heme proteins for the study of the diptheria pathogen: Courtni E. Allen and Michael P. Schmitt. Utilization of Host Iron Sources by Corynebacterium diphtheriae: Multiple Hemoglobin-Binding Proteins Are Essential for the Use of Iron from the Hemoglobin-Haptoglobin Complex. J. Bacteriol. February 2015 vol. 197 no. 3 553-562. doi: 10.1128/JB.02413-14...Cell debris was removed by centrifugation at 12,000 × g at 4°C, and the supernatant fraction containing the soluble protein was purified on Strep-Tactin columns (Neuromics) for Strep-tagged constructs...

Figure: (A) HtaA and derivatives were assessed for the ability to bind Hb-Hp by ELISA. GST was included as a negative control. Experiments were repeated at least three times, with similar results. Results of a representative experiment are shown. (B) Binding of HtaA and various derivatives to Hb-Hp at a 200 nM concentration is shown. Binding of HtaA-Y361A and CR2-Y361A to Hb-Hp was significantly different from binding with HtaA-wt and CR2, respectively (**, P less than 0.0001). Binding of CR1 to Hb-Hp was significantly different from that with HtaA-wt (***, P less than 0.05). *, HtaA does not bind Hp (HtaA-Hp). Values represent the means from three independent experiments (±SD).

Looking for protein purification solutions? Do not hesitate to contact me: 612-801-1007 or pshuster@neuromics.com.

Friday, July 29, 2016

Large Intestine Endothelial Cells

"Hard to Find", Potent and Pure

We asked one of our Large Biopharma users of our Large Intestine Endothelial cells, "Why Neuromics" and she told us, "It is hard to find these cells. Let alone ones that work as well as yours!"


We have many options for endothelial cells and pericytes. We also have the ability to microdissect out virtually any type of human cell. If you have need, just ask me. Direct cell: 612-801-1007 or pshuster@neuromics.com . Pete Shuster, CEO and Owner, Neuromics.

Thursday, July 21, 2016

Human Endothelial Cells and Drug Discovery

Pure, Potent and Culture Ready
Alterations of endothelial cells and the vasculature play a central role in the pathogenesis of a broad spectrum of the most dreadful of human diseases, as endothelial cells have the key function of participating in the maintenance of patent and functional capillaries. This makes them a key component for Drug Discovery.

We are proud that Biotech and Biopharma Cos. are increasingly using our cells. We believe we represent the best options. Here're some of the cells currently available:
Microvascular Endothelial Cells in Action

Figures: HRMECs treated with SUZ12 siRNA demonstrate increased miR-200b expression, decreased VEGF expression and decreased endothelial branching. A,B: Gene knockdown of EZH2 and SUZ12 was confirmed by qPCR. C,D: In HRMECs transfected with EZH2 siRNA in HG, miR-200b and VEGF were not significantly different from HG+control siRNA but decreased compared to NG+control siRNA. In HRMECs transfected with SUZ12 siRNA in HG, miR-200b was significantly increased with decreased levels of VEGF compared to HRMECs transfected with control siRNA, with levels similar to NG+control siRNA. E,F: Tube formation assay to measure endothelial branching. HRMECs transfected with HG+control siRNA demonstrated significantly increased branching compared to NG+control siRNA. Transfection of EZH2 siRNA did not reduce endothelial branching significantly compared to HG+control siRNA. However, transfection of SUZ12 siRNA significantly reduced endothelial branching compared to HG+control siRNA. 10.1371/journal.pone.0123987
We will keep you posted as we add more cell options.

Saturday, July 09, 2016

Immortalized hMicroglia Now Available

In Stock and Ready to Culture!

One of our partners needed 10 million hMicroglia to assay TBI related auto-antibodies as key piece of there target discovery program. We were unable to find these cell available on the market. We decided to have them micro-dissected from human brain tissue and then immortalized.

This enabled us to answer the bell. We now make them available to you. We are offering 2000 USD off through August 31, 2016...only 2995 USD/1,000,000 immortalized cells.
Image: Immortalized Human Microglia in Culture.
Cells are cyropreserved and delivered frozen.

Questions? e-mail pshuster@neuromics.com or direct phone: 612-801-1007. Pete Shuster, CEO and Owner.


Tuesday, June 28, 2016

Cells Detachment

Easy, Gentle and Complete

I often get asked if trypsin is the best reagent to detach primary and stem cells. The problem with a trypsin is that it could lyse a percentage of the cells.

We recommend our DetachinTM. It offers the following benefits:
  • Gentle and rapid detachment.
  • Maximum cell viability.
  • Effective on a wide range of cells.
  • No mammalian or bacterial byproducts.
  • Stable at 4°C for 2 months.
  • No need to wash detached cells.
Here's a recent publication referencing the use of Detachin: Yu Hou, Qi Feng, Miao Xu, Guo-sheng Li, Xue-na Liu, Zi Sheng, Hai Zhou, Ji Ma, Yu Wei,1 Yuan-xin Sun,Ying-yi Yu, Ji-hua Qiu, Lin-lin Shao, Xin-guang Liu, Ming Hou, and Jun Peng. High-dose dexamethasone corrects impaired myeloid-derived suppressor cell function via Ets1 in immune thrombocytopenia. Blood 2016 :blood-2015-10-674531; doi:10.1182/blood-2015-10-674531 ...Both PBMCs and splenocytes were cultured in complete medium for 7 days. Each culture was supplemented with recombinant human IL-6 (10 ng/mL; R&D Systems, Minneapolis, MN) and granulocyte macrophage-colony-stimulating factor (10 ng/mL; R&D Systems) in the presence or absence of DXM and incubatedin humidified air with 5%CO2 at 37°C. Cells cultured in medium alone were runin parallel as controls. Adherent cells were removed using a nonprotease cell detachment solution Detachin (Neuromics, Edina, MN). CD331 cells were isolated by anti-CD33 magnetic microbeads and LS column separation (Miltenyi Biotec, Bergisch Gladbach, Germany), per manufacturer's instructions. The purity of the isolated cells was found to be higher than 90% by flow cytometry

Figure 1. The number of MDSCs and their expression of Arg-1/iNOS in the peripheral blood. (A) The representative scatter-gram of CD11b1CD331HLA-DRlow cells within the gate of PBMCs. Histograms of Arg-1 and iNOS in CD11b1CD331HLA-DRlow cells from healthy control patients and patients with ITP before any treatment was initiated. (B) The percentage of CD11b1CD331HLA-DRlow cells in hemolyzed whole blood from primary active patients with ITP (n 5 21) and healthy control patients (n 5 18). (C-D) The expression (mean fluorescence intensity) of Arg-1 (C) and iNOS (D) in circulating MDSCs compared between patients with ITP (n 5 21) and control patients (n 5 18). Significance between the 2 groups was determined by Student-Newman-Keuls test.
We are working hard to find new ways for you to optimize your cell based assays.

Cells Detachment

Easy, Gentle and Complete

I often get asked if trypsin is the best reagent to detach primary and stem cells. The problem with a trypsin is that it could lyse a percentage of the cells.

We recommend our DetachinTM. It offers the following benefits:
  • Gentle and rapid detachment.
  • Maximum cell viability.
  • Effective on a wide range of cells.
  • No mammalian or bacterial byproducts.
  • Stable at 4°C for 2 months.
  • No need to wash detached cells.
Here's a recent publication referencing the use of Detachin: Yu Hou, Qi Feng, Miao Xu, Guo-sheng Li, Xue-na Liu, Zi Sheng, Hai Zhou, Ji Ma, Yu Wei,1 Yuan-xin Sun,Ying-yi Yu, Ji-hua Qiu, Lin-lin Shao, Xin-guang Liu, Ming Hou, and Jun Peng. High-dose dexamethasone corrects impaired myeloid-derived suppressor cell function via Ets1 in immune thrombocytopenia. Blood 2016 :blood-2015-10-674531; doi:10.1182/blood-2015-10-674531 ...Both PBMCs and splenocytes were cultured in complete medium for 7 days. Each culture was supplemented with recombinant human IL-6 (10 ng/mL; R&D Systems, Minneapolis, MN) and granulocyte macrophage-colony-stimulating factor (10 ng/mL; R&D Systems) in the presence or absence of DXM and incubatedin humidified air with 5%CO2 at 37°C. Cells cultured in medium alone were runin parallel as controls. Adherent cells were removed using a nonprotease cell detachment solution Detachin (Neuromics, Edina, MN). CD331 cells were isolated by anti-CD33 magnetic microbeads and LS column separation (Miltenyi Biotec, Bergisch Gladbach, Germany), per manufacturer's instructions. The purity of the isolated cells was found to be higher than 90% by flow cytometry

Figure 1. The number of MDSCs and their expression of Arg-1/iNOS in the peripheral blood. (A) The representative scatter-gram of CD11b1CD331HLA-DRlow cells within the gate of PBMCs. Histograms of Arg-1 and iNOS in CD11b1CD331HLA-DRlow cells from healthy control patients and patients with ITP before any treatment was initiated. (B) The percentage of CD11b1CD331HLA-DRlow cells in hemolyzed whole blood from primary active patients with ITP (n 5 21) and healthy control patients (n 5 18). (C-D) The expression (mean fluorescence intensity) of Arg-1 (C) and iNOS (D) in circulating MDSCs compared between patients with ITP (n 5 21) and control patients (n 5 18). Significance between the 2 groups was determined by Student-Newman-Keuls test.
We are working hard to find new ways for you to optimize your cell based assays.

Monday, June 20, 2016

MeCP2 and Nerve Injury

i-Fect used In Vivo for Study

Researchers use our i-FectTM to effectively deliver miR-126 in vivo to modulate Methyl-CpG-binding protein 2 (MeCP2).

MeCP2 regulates gene expression through activation, repression and chromatin remodeling. Mutations in MeCP2 cause Rett syndrome, and these patients display impaired nociception. The researchers observed an increase in MeCP2 expression in mouse dorsal root ganglia (DRG) after peripheral nerve injury: Melissa T. Manners, Adam Ertel, Yuzhen Tian and Seena K. Ajit. Genome-wide redistribution of MeCP2 in dorsal root ganglia after peripheral nerve injury. Epigenetics & Chromatin 20169:23. DOI: 10.1186/s13072-016-0073-5© The Author(s) 2016 Received: 11 March 2016. Accepted: 27 May 2016Published: 7 June 2016...miRNA administration protocol was adapted from previous report of intrathecal miRNA delivery. To administer miRNA mimics, a polyurethane catheter (25G, 5.5 cm long, SAI infusion) was placed into the intrathecal space of the lumber L4–L5 vertebrae under isoflurane anesthesia. The catheter was stereotactically secured under the skin and occluded between injections. A custom miRCURY (Exiqon) miR-126 mimic containing a 5′ cholesterol tag and 3′ fluorescein label was injected at 2 nmol concentration with 4 µl iFECT transfection reagent (Neuromics). A total of 6 µl was delivered into the catheter connection juncture using a 25G blunt end needle on a Hamilton syringe. The catheter was then flushed with 7 µl sterile PBS to ensure miRNA reached the intrathecal space...

Figure: Expression of miR-126 and its target genes Dnmt1 and Vegfa in the DRG after nerve injury. a Relative expression of miR-126 determined by qPCR shows a reduction in miR-126 in SNI model compared to DRG from sham control. U6 was used for normalization (n = 8 sham, n = 7 SNI). b Relative expression of Dnmt1 mRNA and c Vegfa transcripts showed an increase in the DRG after nerve injury compared to control (n = 3). Gapdh was used as a normalizer. d Representative Western blot and quantification showed an increase of Dnmt1 protein in the DRG after nerve injury. e Western blot and quantification showed Vegfa protein was not significantly different in DRG after nerve injury (n = 3 from pooled samples, three DRG were pooled for each sample).


Conclusions: The study shows a regulatory role for MeCP2 in that changes in global redistribution can result in direct and indirect modulation of gene expression in the DRG. Alterations in genome-wide binding of MeCP2 therefore provide a molecular basis for a better understanding of epigenetic regulation-induced molecular changes underlying nerve injury.

Sunday, June 12, 2016

Neuroscience Cell Based Markers Applications

Progenitors, Neuronal, Astroglia and PNS Markers A to Z
Our Neuron/Synapse, Astrocytes, Glia, Microglia, Olidogodendrocytes, Progenitors and Schwann Cell Markers continue to widely used and frequently published.
Here're some recent examples: Cinzia Ambrosi , Cynthia Ren, Gaelle Spagnol, Gabriel Cavin, Angela Cone, Elena E. Grintsevich, Gina E. Sosinsky, Paul L. Sorgen. Connexin43 Forms Supramolecular Complexes through Non-Overlapping Binding Sites for Drebrin, Tubulin, and ZO-1. Published: June 9, 2016. http://dx.doi.org/10.1371/journal.pone.0157073...chicken anti-GFAP in blue (Neuromics, Catalog # CH22102)...
Figure 1. Cx43 and drebrin colocalization analysis in brain and cellular models. (A) Rat brain transversal slice mosaic shown after multiple immunolabeling with antibodies anti-Cx43 (red), anti-drebrin (green), and anti-GFAP (blue) as astrocytes marker. White boxes localize the area enlarged in insets 1, 2, and 3 (six fold enlargement). Colocalization of drebrin and Cx43 (yellow) is especially noticeable around the blood vessels (inset 2) and in regions rich of astrocytes (insets 1 and 3). The different regions of the brain were labeled. Cultured astrocytes (B and C) and Vero cells (D and E) were immunolabeled with anti-Cx43 (red), anti-drebrin (green), and anti-actin (blue). White arrows indicate zones of colocalization of Cx43, drebrin and actin that were enlarged in the insets (white boxes, three fold enlargement).

Xiangchen Li, Yu Guo, Yaxin Yao, Jinlian Hua, Yuehui Ma, Changqing Liu, Weijun Guan.Reversine Increases the Plasticity of Long-Term Cryopreserved Fibroblasts to Multipotent Progenitor Cells through Activation of Oct4. International Journal of Biological Sciences 2016; 12(1): 53-62. doi: 10.7150/ijbs.12199...anti-GFAP and NSE (1:200,Neuromics, MN, USA)...
Save 70 USD on High Titer Neuron/Synapse Markers-Only 225/100 ul (Through June 30, 2016).
We will continue to post news on our Neuroscience Research Solutions!

Friday, June 03, 2016

Human Astroglia and Schwann Cells-BBB Model

Broadening our Capabilities

At the core of our solutions are many options for primary human cells. We are especially pleased that we have growing capabilities to provide new cells to researchers studying autoimmune neuro-degenerative diseases like ALS and MS with the addition of:
Schwann Cells
Human Schwann Cells (HSwC) are isolated from human spinal nerve. HSwC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HSwC are characterized by immunofluorescence with antibodies specific to S100, GFAP, and CD90. HSwC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HSwC can to further expand for 10 population doublings in our Schwann Growth medium (cat # SGM001).
Human Astrocytes
 Human Brain Astrocytes cultured with AlphaBioCoat.
Human Blood Brain Barrier Model
I will continue to post updates here.

Wednesday, May 18, 2016

IGF and Mood

Gender Specific?

IGF is a known mood modulator. This paper hypothesizes that the impact of increased IGF is sex dependent: Victor Munive, Andrea Santi and Ignacio Torres-Aleman. A Concerted Action Of Estradiol And Insulin Like Growth Factor I Underlies Sex Differences In Mood Regulation By Exercise. Scientific Reports 6, Article number: 25969 (2016) doi:10.1038/srep25969.

Our IGF-I R/CD221-Fluorescein Labeled was used to link Estrodial with elevating IGF-I levels in female mice.
Images: (A) Cultured brain endothelial cells accumulate significantly more IGF-I after treatment with 10−10M estradiol (E2). Representative blot is shown (*p less than 0.05 vs control; n = 8 per group). (B) The effects of E2 were mediated by α E2 receptors as only the α inhibitor MPP blocked E2 actions. Representative blots are shown (*p less than 0.05; n = 8). (C) Levels of IGF-I receptor (green) at the cell membrane of endothelial cells are markedly increased three hours after addition of 10−10M E2.

Summary: Sex-specific responses to physiological neuroprotective stimuli such as physical activity, that modulate mood in part through modulation of endocrine signals, contribute to sex differences in mood homeostasis. Hopefully, a better understanding of these differences will help us gain insight of sex differences in the incidence of mood disorders.

Friday, May 13, 2016

Cancer-Associated Fibroblasts and Cancer Cell Migration

Highway for Cancer Metastasis

Cancer Associated Fibroblasts provide the Matrices  (ECMs) serve as cancer’s assistants as it spreads throughout the body.



Cancer cells (red) migrate on a CAF-derived extracellular matrix (green). VANDERBILT UNIVERSITY; BEGUM ERDOGAN, DONNA WEBB

Unlike other ECMs, which normally form dense meshes, these arrange into parallel bundles forming the highway for tumor cell migration (The Scientist-December 15, 2015).

Monday, May 02, 2016

New Neuronal Astroglial PNS Markers

Save 70 USD on High Titer Antibodies
The foundation of our company is built on our catalog of proven, published and high titer Neuron-Astroglia and PNS Markers. We are pleased to offer 75 USD off our latest additions.
Antibody
Type
Species Reactivity
Applications
Mouse IgG
Chicken IgY
Chicken IgY
Mouse IgG
Mouse IgG
Mouse IgG
Mouse IgG
Mouse IgG
Chicken IgY
Rabbit IgG
Mouse IgG
B; H; M; R
B; H; M; R
H; M; R
B; H; M; R
B; H; M; R
H; R
Ch; H; M; Pr; R
Ch; H; M; R
H; M; R
H; M; R
B; H; M; R
IF; WB
ICC; IF; IHC; WB
ICC; IF; IHC; WB
IF; WB
IF; WB
IF; WB
ICC;
IHC; WB
ICC; IHC; WB
ICC; IF; IHC; WB
ICC; IF; IHC; WB

Images: Left: View of mixed neuron/glial cultures stained with Aldolase-C (green) and our rabbit antibody to NeuN/FOX3 (red). MCA-4A9 antibody reveals strong cytoplasmic staining in astrocytes, while Rabbit Fox3/NeuN antibody shows nuclear and distal cytoplasmic staining in neuron cells and is complete absence of astrocytes. Blue is a DNA stain. Middle and Right: Mouse brain sections (fixed by transcardial perfusion with 4% paraformaldehyde) stained with Aldolase-C (red) and our chicken Vimentin antibody (green). In the striatum (Middle), Aldolase-C positive astrocytes are highly co-stained Vimentin, which results in yellow to gold colors. In the cerebellum (Right), however, Aldolase-C positive Purkinje cells do not express vimentin, which results in red color. Insets show a higher magnification picture of MCA-4A9 single labeling in red. Nuclei are labeled with DAPI (blue).
Our clients often use these antibodies for double and triple labeling like the above example.


We plan on aggressively be adding more and more of these type of antibodies. Stay tuned.

Tuesday, April 26, 2016

ELISA Blocking Buffers

Paramount to Reducing Background Noise and Attaining an Accurate Signal

Once the antibody or protein antigens have been properly adsorbed onto the ELISA/EIA plate, the next critical step in creating a reliable immunoassay is the blocking of the plate. This key is:

  • Preventing nonspecific binding 
  • Reducing ELISA background signal 
  • Blocking nonspecific binding to adsorbed proteins 
  • Stabilizing proteins adsorbed to plate for better interactions 



Neuromics's ELISA Blocking Buffer formulations reduce nonspecific binding of sample and assay components to the ELISA well while stabilizing the coated protein. Six formulations offer significant benefits to different assay situations. Browse the product selections below, or order our Blocking Buffer Optimization Pack to try three formulations at an economical rate.

Friday, April 15, 2016

Isolating and Selecting T-Cells

Save 100 USD on New Kits

We are pleased to announce the addition of our New T-Cell Isolation/Selection Kits. They are fast and easy. Check out the Short Protocol.

Figure CD4+ T-Cell Isolation Process doi:10.1371/journal.pone.0035798.

We also have T-Cell Expansion Kits!
NameCatalog #TypeSpeciesApplicationsSizePrice
StrepMan Magnet for 15ml and 50ml Tubes6-5650-065Cell Isolation-SeparationHCell Assays1 Magnet$400
CD3 Fab Streptamer Isolation Kit MB6-8000-201Cell Isolation-SeparationH1 Kit$699
CD4 Fab Streptamer Isolation Kit MB6-8000-206Cell Isolation-SeparationH1 Kit$699
CD8 Fab Streptamer Isolation Kit MB6-8000-203Cell Isolation-SeparationH1 Kit$699
CD25 Fab Streptamer Isolation Kit MB6-8000-207Cell Isolation-SeparationH1 Kit$699
We'll be adding more and more kits in the coming months and will keep you posted.