Friday, March 23, 2018

Give it a Try

You will be Satisfied
Our FBS is growing in popularity over the past year. In follow up with users, we learned that our low price catalyzed their trying, but its potency resulted in high levels of satisfaction.

Here are some recent reviews:

  • I enjoyed working with the people at Neuromics. The sale person was very friendly and willing to take extra time to solve our problems. Organization: Mayo User: Wenqian Hu - Oct 15, 2017 - Rating: 5.0 
  • Great Service. Got the products in a very prompt manner! Kemin AgriFoods North AmericaVM - Jul 27, 2017 - Rating: 4.0 
  • Fast and easy supplier to work with! Organization: Fresenius KabiChris - Jun 29, 2017 - Rating: 4.0

Our FBS is 9CFR-tested, meeting FDA and USDA requirements. Fetal bovine serum products can also be tested to meet EMEA requirements. We source our FBS cell culture medium supplement from the United States.
Vascular Smooth Muscle Cells cultured in media supplemented with our FBS
We are offering you our FBS for 299 USD/500 ml. Just use the promo code "April". We offer 100% refunds should it not work as expected.

Thursday, March 15, 2018

Great Growth Factors

Potent and Proven

Our growth factors continue to be referenced in leading publications.  They are excellent for amping up your stem-cell expansion and differentiation media-Tom Kamperman, Sieger Henke, Claas Willem Visser, Marcel Karperien, Jeroen Leijten. (2017). Centering Single Cells in Microgels via Delayed Crosslinking Supports Long-Term 3D Culture by Preventing Cell Escape. Small, doi: 10.1002/smll.201603711.
We will continue to post updates.

Thursday, March 01, 2018

HUVECS in 3-D Action

Form Vascular Networks in Microfluidics Model
In this study, the authors developed a 3D functional human microvascular network in a microfluidic device. The established model enables Neuromics GFP-labeled human umbilical vein endothelial cells to form vessel-like microtissues and have physiological functions which are closer to cells in human blood vessels. The perfusable microvasculature allows the delivery of nutrients, and oxygen, as well as flow-induced mechanical stimuli into the luminal space of the endothelium. The microflow effectively mimic the blood flow in human vessels.

This in-vivo like model is then used for toxicity assays-Yan Li, Qing-Meng Pi, Peng-Cheng Wang, Lie-Ju Liu, Zheng-Gang Han,Yang Shao, Ying Zhai, Zheng-Yu Zuo, Zhi-Yong Gong, Xu Yang and Yang Wu. Functional human 3D microvascular networks on a chip to study the procoagulant effects of ambient fine particulate matter. : RSC Adv., 2017, 7, 56108
Images: Microvascular network formation based on microfluidic 3D HUVEC culture. (A) Schematic diagram of a microfluidic device. (B) Schematic diagram of microvascular network formation based on microfluidic 3D HUVEC culture. (C) Schematic diagram of loading microparticles in microvascular networks. (D) Microscope image of HUVECs seeding in fibrin hydrogel. (E) Confocal microscope image of fluorescent microvascular networks.
Our human primary and stem cells are widely used and frequently published. We will continue to post relevant results from researchers using the cells here.

Thursday, February 22, 2018

Primary Human Astrocytes vs Derived Astrocytes Cell Lines

Potent, Pure and Easy to Culture

We are often asked how our human primary astrocytes stack up versus engineered astrocyte cell lines. Astrocyte cultures, whether primary or engineered, need to mimic how they work in-vivo.

This publication is a comprehensive study of the capabilities of our primary astrocytes versus engineered cells. Anders Lundin, Louise Delsing, Maryam Clausen, Piero Ricchiuto, José Sanchez, Alan Sabirsh, Mei Ding, Jane Synnergren, Henrik Zetterberg, Gabriella Brolén, Ryan Hicks, Anna Herland, and Anna Falk. (2018). Human iPS-Derived Astroglia from a Stable Neural Precursor State Show Improved Functionality Compared with Conventional Astrocytic Models. Stem Cell Reports. DOI:

With the exception of glutamate uptake, our primary cells talk and walk like astrocytes. We do plan on running assays to test for glutamate uptake as we believe the cells are capable.

It is important that all our human neurons, astrocytes, microglia and schwann cells are potent, pure and easy to culture. As you can see from this graphic, our "primary astros" our positive for most the required characteristics.

Monday, February 19, 2018

i-Fect Delivers Again and Again

Silencing Lactate Dehydrogenase A in vivo

Pathologic CNS is characterized by neuronal damage that leads to the release of intracellular components. However, the effect of damaged cells on angiogenesis has not been clarified. This study revealed that LDHA, which is a known damage marker, promotes CNS-specific angiogenesis. LDHA-mediated angiogenesis depends on vimentin on the surface of vascular endothelial cells. The work described here proposes a novel mechanism by which neurodegeneration drives angiogenesis in the CNS.

A mixture of our i-FectTM and LDHA siRNA, in this study, were directly injected into mice cortexes: Hsiaoyun Lin, Rieko Muramatsu, Noriko Maedera, Hiroto Tsunematsu, Machika Hamaguchi, Yoshihisa Koyama, Mariko Kuroda, Kenji Ono, Makoto Sawada, Toshihide Yamashita. Extracellular Lactate Dehydrogenase A Release From Damaged Neurons Drives Central Nervous System Angiogenesis.
Images: LDHA is sufficient to evoke CNS angiogenesis. (a) Representative images of CD105-labeled spinal cord sections obtained 7 days after LDHA administration. (b) Length of CD105+ neovessels around the LDHA administration site as indicated in a, n = 5 each. (c) Representative image of a Nissl-stained brain section after controlled cortical impact (CCI). (d) Representative image of the CD105-immunolabelled cerebral cortex obtained 7 days after CCI. (e) Length of CD105+ neovessels around CCI lesions as indicated in d; n = 5 each, all error bars represent the s.e.m. **P < 0.01, Student's t-tests. Scale bars, 200 μm.

The findings reveal unexpected neurovascular interactions in the injured adult CNS that may be relevant to our understanding of neuronal damage, which is a hallmark of many CNS disorders

Thursday, February 15, 2018

iPSC Derived Human Neural Progenitors

Potent, Pure and Easy to Culture

We are pleased to announce the addition of Human Neural Progenitors to our Primary and Stem Cell offering.
Human Neural Progenitors at 95% Confluency
Cell potency, for us, includes the how well our cells can be differentiated into terminal types. For these progenitors, we have protocols for differentiating into neurons, astrocytes, and oligodendrocytes.
Neural Progenitors differentiated into Neurons and Stained with Tuj-1
We also have Neural Progenitors from Alcohol and Opioid-Addicted Donors.

Wednesday, February 07, 2018

Culturing Cells in Defined 3-D Structures

Media Supplements Matter

There have been several publications referencing use of our growth factors in 3-D Cultures. It is important that potent growth factors are used to ensure proper cell growth and differentiation.

Here's a new publication referencing use of our ISOKineTM FGF. Our ISOKine growth factors are produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture.

Claas Willem Visser, Tom Kamperman, Lisanne P. Karbaat, Detlef Lohse and Marcel Karperien. In-air microfluidics enables rapid fabrication of emulsions, suspensions, and 3D modular (bio)materials. Science Advances 31 Jan 2018: Vol. 4, no. 1, eaao1175 DOI: 10.1126/sciadv.aao1175. In this study, the authors present in-air microfluidics (IAMF), a new chip-free platform technology that enables in-flight (that is, on-the-fly) formation of droplets, fibers, and particles and their one-step deposition into 3D constructs with a modular internal architecture.
Figure: Concept of IAMF and guide to the article. (A) Chip-based microfluidics enables in-line control over droplets and particles, making it a versatile platform technology. A chip design where droplets (blue) are transported by a coflow (pink) is shown. (B) IAMF maintains the in-line control of chip-based microfluidics but relies on jet ejection and coalescence into air. Therefore, a wide range of droplets and particles can be produced at flow rates typically two orders of magnitude higher than with chip-based microfluidics. When combining reactive, solidifying microjets, IAMF also enables on-the-fly production and direct deposition of microparticles into 3D multiscale modular (bio)materials.

Figure: One-step additive manufacturing and injection molding of 3D multiscale modular (bio)materials. (A) Modular free forms with a controlled microarchitecture were manufactured by stacking of shape-stable core-shell particles. (B to D) A hollow cylinder was formed by deposition of the composite jet onto a rotating substrate. By altering the building blocks’ composition, the resulting microarchitecture consisted of (C) a liquid-filled foam or (D) a multimaterial modular solid, where the cross-linker for the core was added to the shell and vice versa. (E) To eject a modular filler, only the droplets’ cores are solidified in the air, whereas the slower solidifying shells enable seamless filling of the mold. (F to H) A modular construct was produced by filling a bone-shaped mold. Inset: Hydrogel construct while still in the mold. The 3D multiscale modular material consisted of MSCs (pink), encapsulated in alginate microspheres (green) that are embedded in dextran-tyramine hydrogel (red). (I) Injection-molded multiscale modular tissue construct with optimized cellular micro- and macroenvironments. The construct consisted of insulin-producing pancreatic β cells (MIN6; beige with blue nuclei) that were encapsulated in alginate microparticles (green). The cell-laden microparticles were encapsulated within a proangiogenic fibrin network that contained human endothelial and stem cells (pink with blue nuclei). The microenvironments supported MIN6 cell proliferation, whereas the macroenvironment supported the formation of an endothelial cellular network within 7 days of in vitro culture. HUVEC, human umbilical cord endothelial cell. Scale bars, 1 cm (B and F), 5 mm (G), and 100 μm (C, D, H, and I).

We live in a 3-D world and 3-D Cell and Tissue Based Assays are a major focus for us. This includes bioinks for 3-D printing.

Sunday, February 04, 2018

Human Hepatocytes

From Healthy and Diseased Donors
We continue to add human primary and derived cells important to your research.

We now have Human Hepatocytes. They come from healthy and drug-addicted donors.

Like all our cells, these are potent, pure and easy to culture.
iPSC Derived Heptocytes
Human HepatocytesHC4230  Cell Assays1,000,000 Cells$795.00
Human HepatocytesHC4230  Cell Assays500,000 Cells$595.00
Human Hepatocytes - Alcohol AddictedHC4230AA  Cell Assays1,000,000 Cells$895.00
Human Hepatocytes - Alcohol AddictedHC4230AA  Cell Assays500,000 Cells$695.00
Human Hepatocytes - Opioid AddictedHC4230OP  Cell Assays1,000,000$895.00
Human Hepatocytes - Opioid AddictedHC4230OP  Cell Assays500,000$695.00

Here's to exciting discoveries.

Wednesday, January 31, 2018

Studying Apoptosis

By Cancer Researchers

Our Apoptosis Kits have proven rock solid in the hands of cancer researchers.

Here's data from a study of the impact of Vestibular schwannoma (VS) on hearing loss.
Images: Spiral Ganglion Cells labeled with our polycaspase kit stain to identify apoptotic cells.

Tuesday, January 23, 2018

Culturing Neurons on Conductive Biomaterials

Neuromics' Neurons on Graphene
Conductive biomaterials are an ideal bio-substrate for modifying cellular behaviors by conducting either internal or external electrical signals.

In this study, researchers successfully culture our cortical neurons on Nonfunctionalized graphene nanosheets (NGN): Shiyun Meng, Rong Peng. Growth and Follow-Up of Primary Cortical Neuron Cells on Nonfunctionalized Graphene Nanosheet Film. Article first published online: January 18, 2018.
Figure: Spray-coating a nonfunctionalized graphene nanosheet (NGN) on a glass slide. To fix the NGN onto the glass slide, polyurethane (PU) was firstly spin-coated onto glass slides as a polymer matrix, then NGN particles were spray-coated on by airbrush.
Figure: Nuclei formed in 7 and 14 days of cell culture are shown at a relatively high magnification (scale bar = 50 μm): 1/500 MAP-2 (H-300) and 1/500 Alexa Fluor® 488 anti-Rabbit stained green for neuron microtubules and DAPI stained blue neuron nuclei.

We have a large catalog of potent, proven and pure human and animal cells.

Monday, January 15, 2018

Cells from Diseased Donors

Focus on Neuro Diseases

We now have the capability to provide cells of the central and peripheral nervous system from donors with Neuro diseases. These include cells from donors with ALS, AD, PD, and Brain Cancer Donors, to name a few.

We have provided cells to virtually all the large Pharmas and many small and mid-size Bio-techs. We have worked with Novartis to gain 21-CFR compliance for cells that they are using for their eye diseases drug discovery programs.

I am at your “beck and call” should you have interested in exploring specific capabilities further. You can e-mail or call me at 612-801-1007

Tuesday, January 09, 2018

Microvascular Endothelial Cells

Tested, Characterized and Research Ready
Our Microvascular Endothelial Cells continue to work and work in the hands of our customers.

Check out these pubs:
1. Odunayo O. Mugisho, Colin R. Green, Jie Zhang, Nicolette Binz, Monica L. Acosta, Elizabeth Rakoczy and Ilva D. Rupentha. (2017). Immunohistochemical Characterization of Connexin43 Expression in a Mouse Model of Diabetic Retinopathy and in Human Donor Retinas. Int. J. Mol. Sci. doi: 10.3390/ijms18122567
 2. Michael Anthony Ruiz, Biao Feng, and Subrata Chakrabarti. (2015). Polycomb Repressive Complex 2 Regulates MiR-200b in Retinal Endothelial Cells: Potential Relevance in Diabetic Retinopathy. PLoS One.10(4): e0123987. doi: 10.1371/journal.pone.0123987.

In these, our cells are used as "healthy controls" to study Diabetic Retinopathy.

Figure: Connexin43 (green) and GFAP (red) expression in normal and human DR donor retinas in regions of extensive vascular damage. Large cells (white arrows, left column) represent non-specific auto-fluorescent amacrine cells. Connexin43 expression was markedly higher in the GCL of DR donor tissues compared to age-matched controls, and was strongly expressed throughout all retinal layers. GFAP labeling was also markedly higher in DR compared to normal donor eyes representing hyper-reactive Müller cells. Connexin43 expression was increased in regions identified as blood vessels and correlated with increased GFAP labeling at these sites, indicating glial cell activation (white circle). GCL = ganglion cell layer; IPL = inner plexiform layer; INL = inner nuclear layer; OPL = outer plexiform layer; ONL = outer nuclear layer. Scale bar: 200 µm
We stand ready to serve you. Pete Shuster, CEO and Owner,

Wednesday, January 03, 2018

Medical Grade Soluble Collagen

New Products-New Applications

We are pleased to announce the addition of  Medical Grade Collagen to our Cell Based Assay Solutions. Applications include:
  • Tissue engineering 
  • Wound healing
  • Medical device coatings
  • 3D cell cultures
  • Drug delivery 
  • Sealants 
  • Electrospinning
  • Hemostats
  • 3D printing
This example outlines how this collagen can be used for cartilage regeneration from mesenschymal stem cells. Acta Biomater. 2016 Jan;30:212-221. doi: 10.1016/j.actbio.2015.11.024. Epub 2015 Nov 18.

Figure: Fabrication of macroporous woven scaffolds and pellet delivery via the macroporous woven collagen scaffold; (a) Liquid to solid phase transition of collagen molecules via electrocompaction to fabricate electrochemically aligned collagen threads and electrocompacted sheets. (b) Collagen thread is woven around a set of pins and threads are stabilized by crosslinking two collagen sheets on top and bottom of woven part of scaffold. (c) Schema of the final woven collagen scaffold. (d) 1 million MSCs pelletized at 500 ×g for 12 minutes, cultured for 3 days and then transferred in to scaffold holes.
SIGNIFICANCE: Mesenchymal condensation is critical for driving chondrogenesis, making high density cell seeding a standard in cartilage tissue engineering. Efforts to date have utilized scaffold free delivery of MSCs in pellet form. This study developed a macroporous scaffold that is fabricated by weaving highly aligned collagen threads. The scaffold can deliver high density cell condensates while providing mechanical stiffness comparable to that of cartilage. The scaffold also mimicked the arcade-like orientation of collagen fibers in cartilage. A highly robust chondrogenesis was observed in this mesenchymal cell pellet delivery system. Baseline mechanical robustness of this scaffold system will enable delivery of cell pellets as early as three days.

Thursday, December 21, 2017

Cell to Cell Signaling and Depression

ATP and Your Brain
A recent article in Molecular Psychiatrydoi:10.1038/mp.2017.229 elucidates the role of ATP and Neuron-Glial interactions in depression.

This study also features the use of our excellent GFAP markers
Abstract: Extracellular ATP is a widespread cell-to-cell signaling molecule in the brain, where it functions as a neuromodulator by activating glia and neurons. Although ATP exerts multiple effects on synaptic plasticity and neuro-glia interactions, as well as in mood disorders, the source and regulation of ATP release remain to be elaborated. Here, we define Calhm2 as an ATP-releasing channel protein based on in vitro and in vivo models. Conventional knockout and conditional astrocyte knockout of Calhm2 both lead to significantly reduced ATP concentrations, loss of hippocampal spine number, neural dysfunction and depression-like behaviors in mice, which can be significantly rescued by ATP replenishment. Our findings identify Calhm2 as a critical ATP-releasing channel that modulates neural activity and as a potential risk factor of depression.

Wednesday, December 13, 2017

Big Thank You!

Big Year
We exceeded our goals for 2017. By a lot. Both in sales and new products.

I would like to personally thank our customers, collaborators, and friends for making this happen. You made it happen.

I wish Joy to you in 2018!
I wish you exciting, new discoveries, and adventures.

Pete Shuster, CEO and Owner, Neuromics.

Friday, December 08, 2017

Coming Soon Hepatocytes and CSCS

Differentiated from iPSCs

Neuromics is partnering with Javeen Biosciences to bring you Cancer Stem Cells and Hepatocytes derived from donor Induced Pluripotent Stem Cells.

Rodney Nash, Javeen CEO, and his team are working to fill a major gap in cell-based drug discoveries. The lack of cells from specific diseased or ethnic populations. This limits the ability to predict how well a drug candidate will work with a diverse population of patients.

Dr. Rodney Nash, CEO, Javeen Biosciences
Over time, we envision Javeen Biosciences as having the ability to engineer cells from donors that match tightly with the needs of drug hunters.

We ask you to "Think of 8". This video illustrates the power Javeen Biosciences' solutions

Much more to follow...

Wednesday, November 29, 2017

Need Cells? Contest is Now Live

It's Easy! 
Creatively insert your favorite human cell culture(s) anywhere into a picture of your lab for $25 Amazon Gift Card. To enter, email the picture to with a description of the image that includes your name, the lab name and the type of cells being cultured.
First 100 entries are eligible for the gift card.

Grand prize is $500 Visa Gift Card. Only entries that include the culture of Neuromics cells will be eligible for the grand prize.

  • Official Contest Rules: 
  • Only one entry per lab. 
  • The first 100 entries are eligible. 
  • Cells cultured do not have to be Neuromics cells to be eligible for Amazon gift card. 
  • All images should be original work – you should not distribute, or reproduce in any way any copyrighted material, trademarks, or other proprietary information without obtaining the prior written consent of the owner of such rights. 
  • By entering this competition, entrants agree that Neuromics can publicize their name, their lab and their entry. 
  • Entries will be posted on Neuromics’ web page, social media channels and other promotional communications. 
  • All submissions become the sole property of Neuromics and will not be returned to the applicant. Prize restrictions may apply to certain individuals based on their institution’s respective policies; based on this, Neuromics reserves the right to decline a prize to ineligible individuals. 
  • Neuromics reserves the right to make any determinations about prizes, disqualifications, and other concerns as issues arise. 
  • Neuromics reserves the right to amend or withdraw the contest at any time.
Enjoy and good luck!

Tuesday, November 21, 2017

Need Cells? Contest

We're live; Be Creative
We are offering a $25 Amazon Gift Card to scientists who creatively places images their favorite cell cultures anywhere in a picture of their lab AND if the picture has images of our human cells, you could win a $500 VISA card.

Here's a link to learn more about our Need Cells Contest.

Please email your picture to and we will email you a $25 Amazon Gift Card.

Thursday, November 16, 2017

Coming Soon-Need Cells? Contest

Creativity Rewarded
We are revving up another contest as our Neuromics Brain Adventure was successful and wow, were people creative.

We are expert at finding human primary and stem cells so cell cultures are central to the contest. Rules are simple. Affix an image of your favorite cell culture anywhere in your lab and we will email you a $25 Amazon gift card.

The contest will run for 3 months and the most creative entry will receive a $500 Visa Gift Card.
Simply e-mail your entry to and we will email back the gift card. Multiple entries encouraged.

Monday, November 06, 2017

More FBS Data!

Cells+Media Supplemented with our FBS!

We continue to be pleased with the cell culture images our customers are sharing with us-

Neurons are notoriously hard to culture. Here're images of Mouse Neurons cultured in media supplemented with our FBS.
Images: Mouse cortical neurons in culture, using Neuromics heat-inactivated FBS (in plating media for two days). Data courtesy of Dr. Saif at Barrow Neurological Institute.
We continue to offer $50 Amazon Gift Card in return for data you share using any of our solutions. Just email the data to Rose Ludescher, Manager of Customer Satisfaction, and she will email you the gift card.

Monday, October 23, 2017

3-D Retina Organoid Challenge

Proud Sponsor
The National Eye Institute has its sights set on stimulating researchers to move rapidly toward treatments for retinal diseases. We need your scientific expertise: compete in a Challenge to develop a physiologically-competent 3-D retina organoid model.
Check us out on their Sponsor's Page.

Thursday, October 12, 2017

More Neuromics' FBS Related Data

Cells Cultured in Media Supplemented with our FBS
Customers continue submitting data. Here's the latest:
Primary mouse vascular smooth muscle cells stained with smooth muscle alpha-actin in DMEM with Neuromics 10% FBS. Image courtesy of Deng-Fu Guo, University of Iowa.
HEK Cells Grown in Neuromics FBS. Image courtesy of Kavita Shah, Purdue University.
We will continue be posting new FBS related data here.

Saturday, September 30, 2017

Neuromics' FGF-2 for Expanding Human Cells

FGF-2 and Fibrochondrocytes

FGF-2 of FGF-basic is an important a growth factor for many cell-based assays. Neuromics' has rock solid FGFs for supplementing media used to grow cells. Check out publications referencing use of FGF-2.

Here's the latest publication: Yan Liang, Enaam Idrees, Stephen H. J. Andrews, Kirollos Labib, Alexander Szojka, Melanie Kunze, Andrea D. Burbank, Aillette Mulet-Sierra, Nadr M. Jomha & Adetola B. Adesida. Plasticity of Human Meniscus Fibrochondrocytes: A Study on Effects of Mitotic Divisions and Oxygen Tension. Scientific Reports 7, Article number: 12148 (2017) doi:10.1038/s41598-017-12096-x. ...Thereafter the number of viable MFCs were counted using a haemacytometer after trypan blue staining. MFCs were plated at 104 cells/cm2 and cultured in the standard medium described above supplemented with FGF-2 (5 ng/mL; Neuromics, MN, USA, Catalog#: PR80001) and TGFβ1 (1 ng/mL; ProSpec, NJ, USA, Catalog#: cyt-716) under normal oxygen tension (21% O2) at 37 °C in a humidified incubator...
Images: Immunofluorescence analysis of collagen I and collagen II in pellets derived from T1F2-expanded MFCs of four passages after 21 days chondrogenic stimulation under NRX or HYP from one representative donor (male, 20 years old). Blue (DAPI): cells, Red (Texas Red): collagen I, Green (FITC): collagen II. (A) Pellets cultured under NRX, (B) Pellets cultured under HYP from four passages. Scale bar: 100 µm
Our ISO-Kine FGF-2 is especially potent as it virtually endotoxin free.

Thursday, September 21, 2017

i-Fect Delivers Plasmids!

Important for Gene Expression Studies.
I have posted many examples of how our customers use i-FectTM  and other Transfection Solutions for Gene Manipulation Studies. There are also many publications.

Here we feature how i-Fect was used to delivery plasmids to the CNS: Sara Elramah, María José López-González, Matthieu Bastide, Florence Dixmérias, Olivier Roca-Lapirot, Anne-Cécile Wielanek-Bachelet, Anne Vital, Thierry Leste-Lasserre, Alexandre Brochard, Marc Landry & Alexandre Favereaux. Spinal miRNA-124 regulates synaptopodin and nociception in an animal model of bone cancer pain. Scientific Reports 7, Article number: 10949 (2017) doi:10.1038/s41598-017-10224-1...Intrathecal administration of miRNAs and ShRNA To over-express miR-124, we cloned the pre-miRNA sequence of miR-124 into a plasmid. To determine cells expressing this miR-124 encoding plasmid, we added a GFP-coding sequence to the construct under the control of an IRES. Thus, miR-124 over-expressing cells also express GFP. To inhibit synaptopodin expression, we cloned a ShRNA sequence directed against synaptopodin into a plasmid. To determine cells expressing this ShRNA, we added a GFP-coding sequence to the construct under the control of an IRES. Thus, ShRNA expressing cells also expressed GFP. Two micrograms of these plasmids or the corresponding controls, were solubilized in 10 µl of i-Fect reagent (Neuromics, Edina, USA), and injected intrathecally between the L5 and L6 lumbar vertebrae every two days for a total of 3 injections, according to the manufacturer’s instructions and previously published experiments...
Figures: (C and D) Immunostaining of synpo in spinal cord after miR-124 intrathecal injections: only the dorsal horn which receive nociceptive information was quantified (white dash area). Measurement of synaptopodin stained area reveals ability of miR-124 to inhibit endogenous Synpo expression (20/3 and 17/3 denotes number of sections/animals for control and miR-124-injected mice, respectively.
I am confident there will be many more positive reports regarding our Transfection Reagents.

Saturday, September 16, 2017

Dynamics of Stem Cell Differentiation

Differentiation Markers
Neuromics has excellent markers for determining stem cell differentiation.

This new publication references use of our OTX2 marker: Sumin Jang, Sandeep Choubey, Leon Furchtgott, Ling-Nan Zou, Adele Doyle, Vilas Menon, Ethan B Loew, Anne-Rachel Krostag, Refugio A Martinez, Linda Madisen, Boaz P Levi, Sharad Ramanathan. Dynamics of embryonic stem cell differentiation inferred from single-cell transcriptomics show a series of transitions through discrete cell states. eLife 2017;6:e20487. DOI:

Figures: Single-Cell Gene Expression Profiling of mESCs during early germ layer differentiation. (A) Mouse embryonic stem cells (mESCs) were exposed to various differentiation conditions to perturb FGF, WNT, and TGF-beta signaling for up to five days of differentiation. (B) Images of immunostained mESCs undergoing differentiation show cell-to-cell variability in their expression of known germ layer marker genes. (Scale bar = 100 μm).
We will continue to post new developments.

Friday, September 01, 2017

Neuronal Markers

New Pub References 3 Markers
We are recognized for our large catalog of neuronal markers. Our strength, in this area, includes markers designed for pain researchers.

They are widely used and frequently published. This new publication references use of our Guinea Pig Substance P, Guinea Pig PGP9.5 and Chicken NF200 of NF-Heavy. andla, Jagadeesha, Lomada, Santosh Kumara, Jianninga; Kuner, Rohinia, Bali, Kiran Kumar. miR-34c-5p functions as pronociceptive microRNA in cancer pain by targeting Cav2.3 containing calcium channels. Pain: September 2017 - Volume 158 - Issue 9 - p 1765–1779 doi: 10.1097/j.pain.0000000000000971.
Neuromics' PGP9.5 Staining of  Mouse DRGs.
.Neuromics SP and NF200 Staining of Mouse DRGSs

Thursday, August 31, 2017

FBS Works Great for Cell Lines

More Customer Data
We are pleased with the continuing stream of positive feedback on our potent FBS. This includes its supplementing media for cell lines.

Here is an image courtesy of  Kavita Shah of Purdue University.
Image: HEK Cells cultured in Media Supplemented with Neuromics' FBS

We offer the best price anywhere. We will beat all competitive pricing. If you are interested, contact Rose Ludescher, Manager of Customer Satisfaction. email:

Monday, August 21, 2017

Data Trifecta-Cells, Media + FBS

Optimizing your Cell-Based Assays
Researchers need solid proof that the solutions the use will work in their assays. Given the time and effort it takes to culture human primary cells, it is important that the media and supplements used are optimized.

We engineer our cell media and supplements to work with our primary and stem cells. Its potency is tested every day in our cell cultures.

As further proof, we ask customers to share their data with us. We reward them by emailing a 50 USD Amazon gift card. I would like to recognize Emily Rodela from TGEN for sharing the Trifecta of Neuromics' CAFS grown in MSC-Gro Media supplemented with our FBS.
Image: Human CAFS-Pancreatic Stellates cultured in MSCGro™ supplemented with Neuromics' FBS - Fetal Bovine Serum. Courtesy of Emily Rodela, TGEN.

Monday, August 14, 2017

Neuromics' ISOKine bFGF in 3-D Cultures

Works Well in Perfusion Models

Our ISOKineTM FGF is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture.

It is a proven solution for all cell cultures and starts at the low price of  65 USD/10 ug.

Here's a reference of its use in 3-D Cultures: Tom Kamperman, Sieger Henke, Claas Willem Visser, Marcel Karperien, Jeroen Leijten. Centering Single Cells in Microgels via Delayed Crosslinking Supports Long-Term 3D Culture by Preventing Cell Escape. DOI: 10.1002/smll.201603711.

Figure: Delayed on-chip crosslinking enables centering of single cells in microgels. a) Fluorescence confocal imaging confirmed that delayed enzymatic crosslinking enabled centering of single MSCs in Dex-TA microgels. b) On average, cell-laden microgels were only 9 µm larger than the encapsulated MSCs, effectively resulting in 3D hydrogel coatings of less than 5 µm. c) A standard microfluidic droplet generator was connected to the H2O2 diffusion-based crosslinking chip. The position of cells (white arrows) in non-crosslinking microgel precursor droplets was analyzed d) immediately after droplet generation (t1), at the start of the crosslinking chip (t2), and e) at the end of the crosslinking chip (t3). f) Cell positions within microgels produced using conventional microfluidic encapsulation systems (i.e., with coupled emulsification and gelation) are indicated with gray (i.e., references) and red (i.e., this work) data points. Cell positions within gel precursor droplets along the modular microfluidic setup are indicated with blue data points. Cell positions within delayed enzymatically crosslinked microgels are indicated with green data points. g) Cell position analyses of various combinations of distinct hydrogel materials (i.e., Dex-TA, Dex-HA-TA, PEGDA), cell types (i.e., MIN6, MSC), and crosslinking methods (i.e., enzyme-based and photo-crosslinking), revealed that delayed crosslinking consistently resulted in significantly increased cell-centering as compared to the conventional encapsulation approach where emulsification and gelation are coupled.

If you are looking for competitively priced, animal free and potent growth factors. check out our ISOkines.

Wednesday, August 09, 2017

Cancer Associated Fibroblasts in Action

Publication Reference
We introduced our Cancer Associated Fibroblasts to researchers several years ago. The demand was strong from day one and continues to grow.

We are pleased to announce the first reference in a Cancer Research Publication: Zenobia D'Costa, Keaton Jones, Abul Azad, Ruud van Stiphout, Su Y Lim, Ana L Gomes, Paul Kinchesh, Sean C Smart, W. Gillies McKenna, Francesca M Buffa, Owen J. Sansom, Ruth J. Muschel, Eric O'Neill and Emmanouil Fokas. Gemcitabine-induced TIMP1 attenuates therapy response and promotes tumor growth and liver metastasis in pancreatic cancer. Published OnlineFirst August 1, 2017 doi: 10.1158/0008-5472.CAN-16-2833...Immortalized Pancreatic CAF-Stellate Cells were obtained from Neuromics, and cultured in VitroPlus III, low serum, complete (Neuromics)...
Image: Pancreatic CAF-Stellates stained with alphaSMA

We will continue to post new developments here.