Monday, July 22, 2019

Thursday, July 11, 2019

Rodent Cortical Astroglia

Controls for iPSC Derived Astroglia
We have been providing Neuroscientists rodent neurons and astroglia for many years. As a result, they have been frequently referenced in publications.

Here's a recent publications. In this study. the authors use our rat atsroglia as controls to determine homogeneity of iPSC derived cells.

P. Ni, H. Noh, Z. Shao, Q. Zhu, Y. Guan, J.J. Park, F. Arif, J.M. Park, C. Abani, C. Beaudreault, J.S. Park, E. Berry, A. Moghadam, P. Stanton, J.N. Hutchinson, B. Andrews, C. Faux, J. Parnevelas, L.M. Eisenberg, K. Park, V.Y. Bolshakov, S. Chung. (2019). Large-Scale Generation and Characterization of Homogeneous Populations of Migratory Cortical Interneurons from Human Pluripotent Stem Cells. Molecular Therapy-Methods & Clinical Development, 13:414-430. doi: 10.1016/j.omtm.2019.04.002.

Figure: CDP Treatment Enhances Migratory, Morphological, and Electrophysical Maturation (A) Analysis scheme for migration, arborization, and electrophysiology of cINs. (B) CDP treatment significantly increased the migration of generated iPSC cINs. cIN organoids were embedded in a Geltrex matrix at 9 weeks of differentiation with or without CDP treatment and analyzed for migration 7 days after embedding. White scale bars, 200 μm; yellow scale bars, 100 μm.

Need Cells? Just ask us. Rose Ludescher, Manager of Customer Satisfaction, rose@neuromics.com or 866-350-1500.

Sunday, July 07, 2019

Potent and Proven TUJ-1 Markers

Referenced in Over 50 Publications
Tuj-1 is a key marker for neurogenesis. It can be detected in immature neurons and persists throughout the adulthood. We have 2 options both are widely used and frequently published.
Tuj-1 Chicken Polyclonal
Tuj-1 Mouse Monoclonal
Figure: A-B Representative confocal images of tdTomato+/Myosin7a+ hair cells (asterisks and dashed lines) from early and late tracing associated with Tuj1+ neurites (arrowheads in orthogonal views, n = 127 cells from 3 mice for early tracing and 73 cells from 9 mice for late tracing). Published: July 1, 2019https://doi.org/10.1371/journal.pbio.3000326.


We have great markers for studying neurogenesis.

Thursday, June 27, 2019

FBS-Put it to Work

Priced Right

We provide, to our customers, the same Fetal Bovine Serum (FBS) we use in our defined media. This FBS enriched media is used in culturing all our human 2 and 3-D based Assays. Our FBS is of the highest quality and priced right at $379/500 ml.

Here're some recent publications referencing its use.

  • Gabriela Fernandes, Stephen T Vanyo, Shahad Bakheet Alsharif, Sebastiano Andreana, Michelle B Visser, Rosemary Dziak, Ph.D. Strontium Effects on Human Gingival Fibroblasts. https://doi.org/10.1563/aaid-joi-D-18-00253
  • Douglas Dickinson, Shannon Xayaraj, Sarah Dickinson, Xueling Shao, and Stephen Hsu.  Effect of Novel Formulations using Lipophilic Epigallocatechin3-Gallate against Influenza Virus Infection. Microbiol Infect Dis. 2018; 2(3): 1-8
  • Fei Cao, Li-Xue Yin. (2018). miR-122 enhances sensitivity of hepatocellular carcinoma to oxaliplatin via inhibiting MDR1 by targeting Wnt/β-catenin pathwayExperimental and Molecular Pathology. https://doi.org/10.1016/j.yexmp.2018.10.009
  • Amélie Robert, Peirun Tian, Stephen A. Adam, Mark Kittisopikul, Khuloud Jaqaman, Robert D. Goldman, and Vladimir I. Gelfand. Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport. 26 Jun 2018https://doi.org/10.1096/fj.201800604R
  • Mayuri Manoj Vaidya (2018). Verification of Apoptosis in MDA-MB-231 Triple Negative Breast Cancer Cells Post NBA Photodynamic Therapy Using DNA Fragmentation Assay and Cell Death Dyes. (Doctoral Dissertation). University of Texas San Antonio 


  • And a recent review

    We bought a large order of FBS from Neuromics. Our experience with their customer service was great. They offered us the best price and worked with us on any issue issues that came up on our side.Olesya Plazyo - Mar 12, 2019 - Rating: 5.0

    We offer 50 ml. samples for testing. If interested email rose@neuromics.com.

    Wednesday, June 19, 2019

    Sex Differences on the Behavior of Human Brain Microvascular Endothelial Cells (HBMECS)

    The Risk of Stroke
    There are sex differences in risk for stroke and small vessel ischemic disease in the brain. Microvesicles (MV) derived from activated cells vary by cell of origin and the stimulus initiating their release. MV released from cells activated by inflammatory and thrombotic factors have the potential to disrupt endothelial cells of the brain microvasculature. Therefore, experiments were designed to identify sex differences in the phenotype of MV released from cultured human brain microvascular endothelial cells (HBMEC) in response to inflammatory and thrombotic stimuli (Biology of Sex Differences201910:26 https://doi.org/10.1186/s13293-019-0241-y).

    Neuromics' HBMECS, derived from a male donor, were used to study the differences between Male and Female Cells. There are indeed striking differences in the expression of Cell Adhesion Molecules when the male and female cells were stimulated with inflammatory and thrombotic factors.
    4 Fold increase in release of MV from male (open bars) and female (black bars) HBMEC expressing cell adhesion molecules following stimulation with either TNFα (20 ng/ml) or THR (2 U/ml) for 20 h

    These molecules, in part, control tight junctions in HBMECS. In stroke patients, these are disrupted resulting in injury to the brain.

    The findings  underscore the appropriateness of identifying the sex of cells used in research studies of MV disposition within the vasculature, as well as the sex of cells used to generate MV for in vitro studies

    Wednesday, May 29, 2019

    Human Pancreatic CAFS and Tumor Dynamics

     Extracellular Matrix (ECM) Matters
    Our Human Pancreatic Cancer Fibroblasts were recently featured in a publication focusing on the role of the ECM on tumor dynamics. Andreas Stylianou, Vasiliki Gkretsi, Maria Louca, Lefteris C. Zacharia, and Triantafyllos Stylianopoulos (2019). Collagen content and extracellular matrix cause cytoskeletal remodelling in pancreatic fibroblasts. J. R. Soc. Interface 16: 20190226. http://dx.doi.org/10.1098/rsif.2019.0226.

    Commercially available pancreatic native human FBs and,CAFs (cat. nos. SC00A5 and CAF08, respectively, Neuromics) were cultured in MSC-GRO (VitroPlus III, low serum, complete,
    cat. no. SC00B1, Neuromics) medium in a 5% CO2-incubator at 37OC.


    Figure: Representative CAFs spheroids embedded in collagen gels at time 0 and at 6 h post implantation, respectively.

    The results demonstrated that collagen concentration and consequently ECM stiffening differentially modulates FBs and CAFs cytoskeleton remodelling and invasion with stress fibre orientation being significantly altered in FBs. Finally, the presence of TGF-b reverses the suppressive effect of collagen stiffness on cell invasion.

    Wednesday, May 22, 2019

    Neurofilament Antibodies-Check Them Out

    Widely Used and Frequently Published
    Our Neurofilament Antibodies are well characterized and research ready. Here is a recent publication using one of our Neurofilament Heavy Antibodies. Jennifer M. Hahn, Kelly A. Combs, Christopher M. Lloyd, Kevin L. McFarland, Steven T. Boyce, and Dorothy M. Supp. Identification of Merkel cells associated with neurons in engineered skin substitutes after grafting to full thickness wounds. PLoS One. 2019; 14(3): e0213325. Published online 2019 Mar 5. doi: 10.1371/journal.pone.0213325.
    Figures: Merkel cells in grafted ESS are associated with neurons expression neurofilament heavy (NF-M) by eight weeks after grafting. Immunochemistry with antibodies against NF-H (red) and KRT20 (green) was used to localize neurons and Merkel cells, respectively, in ESS after grafting to mice. Nuclei were counterstained with DAPI (blue; B, D, F, H). Shown are cross sections of ESS at 4 weeks (A-B), 8 weeks (C-D), 12 weeks (E-F), and 14 weeks (G-H) after grafting; each row contains images of the same section. Scale bars in A is same for all images. White arrows indicate examples of NF-H-positive nerves associated with or in proximity to Merkel cells; yellow arrows indicate NF-H-positive nerves not associated with Merkel cells.

    Remember. All our antibodies come with a money back guarantee. Your satisfaction is joh #1 for us. 

    Monday, May 13, 2019

    We Can Sell and Deliver FBS in Large Volumes

    This is Includes Single Lots
    We are pleased with the growth in our FBS business. This is being made possible, in part, to our ability to find the "sweet spot" by coupling low pricing with high quality.

    We offer all potential customers free samples to test with the idea that they find the "sweet spot." This leads to happy customers and repeat business.

    Here's a volume shipment wrapped and ready to go.
    2 pallets of 700X500 ml. Bottles FBS.

    Need FBS? Just ask us. Learn more by emailing rose@neuromics.com. Thank you!

    i-Fect Scores Again

    Knock-down of HIF-1a Attenuates Chemo Induced Pain
    i-Fect TM is one of our original products. It has enjoyed 14 years of upping transfection percentages both in-vitro and in-vivo.

    Here is a new study showing successful use of i-Fect to knock down HIF-1a in-vivo-Taylor Ludman and Ohannes K. Melemedjian. (2019). Bortezomib-induced aerobic glycolysis contributes to chemotherapy-induced painful peripheral neuropathy. Molecular Pain. https://doi.org/10.1177/1744806919837429.


    Figure 1. (a) Treatment of mice with bortezomib (Bor) for five days augmented HIF1A expression in L4-6 DRGs (*P ¼ 0.0412, five mice/ group) relative to the vehicle-treated group. (b) A schematic depicting the site of the intrathecal (IT) siRNA injection. The siRNA was administered between the L4 and L5 vertebrae which is around 17 mm rostral to the spinal cord (SC) section innervated by the L4-6 DRGs. (c) IT injection of siRNA (1 mg in 5 ml) that targets HIF1A (siRNA) but not control siRNA (Cont), for two consecutive days, significantly reduced the levels of HIF1A in L4-6 DRGs. (***P=0.0006, five mice/group). (d) IT siRNA did not affect HIF1A levels in L4-6 spinal cord (five mice/group). (e) After determining baseline withdrawal thresholds using von Frey filaments, male ICR mice received IP injection of vehicle or bortezomib (black arrows) and IT siRNA (blue arrows). The withdrawal thresholds were measured on days 7 to 14. IT HIF1A siRNA prevented the development of bortezomib-induced neuropathic pain. (****P less than 0.0001, five mice/group). DRG: dorsal rootganglia; HIF1A: hypoxia-inducible factor 1 alpha; IT: intrathecal; IP: intraperitoneal; siRNA: small interfering RNA.


    This study is the first to demonstrate that the stabilization of HIF1A expression underpins the development of bortezomib-induced neuropathic pain. Crucially, these findings reveal that the initiation and maintenance of bortezomib-induced neuropathic pain are regulated by distinct mechanisms.

    Saturday, April 27, 2019

    We Have Human Bone Marrow Derived Stem Cells

    Pure and Potent-Only $655/500,000 Cells
    Human Mesenchymal Stem Cells isolated from bone marrow and provided a low passage. These cells have the ability to be passaged five to 10 passages in our Low Serum, Complete Medium (#SC00B1); which is optimized for high growth rates, reduced doubling times, healthy cells and stability. This Product is manufactured, tested and validated in an ISO 9001/ISO13485/CLIA certified facility.

    Tuesday, April 16, 2019

    Gut Brain Axis

    Helicobacter pylori Induced Anxiety and Anorexia
    Our PGP9.5 was used as a Hypothalamic Neuronal Marker in this study-Hajime Suzuki, Koji Ataka, Akihiro Asakawa, Kai-Chun Cheng, Miharu Ushikai, Haruki Iwai, Takakazu Yagi, Takeshi Arai, Kinnosuke Yahiro, Katsuhiro Yamamoto, Yoshito Yokoyama, Masayasu Kojima, Toshihiko Yada, Toshiya Hirayama, Norifumi Nakamura & Akio Inui (2019). Helicobacter pylori Vacuolating Cytotoxin A Causes Anorexia and Anxiety via Hypothalamic Urocortin 1 in Mice. Scientific Reports volume 9, Article number: 6011.

    Images: Ucn1- and DAPI-positive cells (yellow arrow heads), or Ucn1- and PGP9.5-positive cells (white arrow heads) in the PVN cells.

    This is the first study demonstrating the anorexigenic and anxiogenic effects of VacA. The authors propose the following. VacA secreted by Hp in the stomach travels via the peripheral circulation and passes through the BBB. VacA binds to LRP1 and activates the intracellular PLC-PKC pathway, resulting in the activation of Ucn1-positive neurons, such as in the PVN of the hypothalamus. Secreted Ucn1 induces the inhibition of food intake through CRF2 receptors and anxiety through CRF1 receptors (Fig. 6). The central Ucn1-CRF receptor axis activated by VacA might be a new important pathway that contributes to the anorexigenic and anxiogenic effects of Hp infection and could be a therapeutic target for Hp-induced alterations.

    Wednesday, April 03, 2019

    Your Data Wanted

    Reward is $50 Amazon Gift Card

    We are always honored when customers share their data and images. More is better! This month we would like to reward you for any data or images featuring any of our products you share with us.

    Just e-mail Rose Ludescher, Manager of Customer Satisfaction, at rose@neuromics.com , and she will e-mail you a $50 Amazon Gift Card.

    Example Data


    HUMAN PANCREATIC CAF-STELLATE CELLS-Stained with alpha-SMA + DAP1 20x. Courtesy of Emily Rodela, TGEN-Courtesy of Emila Rodela, T-Gen.

    Tuesday, March 19, 2019

    Human and Animal Cardiomyocytes

    Pure, Potent and Easy to Culture

    Check out our hMSC Derived Human Cardiomyocytes-These cells are optimized to provide addition options for in-vitro testing of drug to drug candidate toxicities allowing researchers to rule out the ineffective and potentially small compounds/toxic compounds early in the process.

    Human Cardiomyocytes in Culture

    Neuromics also offers primary rat and mouse cardiomyocyte tissue and disassociated cells.

    Thursday, March 07, 2019

    Neuron-Glial Markers

    Featuring Neurofilament Antibodies
    We have the honor of our Neuron-Glial Markers being referenced in many publications. Here we wanted to feature some recent data from one of our Neurofilament Antibodies.

    Fig. Merkel cells in grafted ESS are associated with neurons expression neurofilament heavy (NF-M) by eight weeks after grafting. Immunochemistry with antibodies against NF-H (red) and KRT20 (green) was used to localize neurons and Merkel cells, respectively, in ESS after grafting to mice. Nuclei were counterstained with DAPI (blue; B, D, F, H). Shown are cross sections of ESS at 4 weeks (A-B), 8 weeks (C-D), 12 weeks (E-F), and 14 weeks (G-H) after grafting; each row contains images of the same section. Scale bars in A is same for all images. White arrows indicate examples of NF-H-positive nerves associated with or in proximity to Merkel cells; yellow arrows indicate NF-H-positive nerves not associated with Merkel cells. https://doi.org/10.1371/journal.pone.0213325.g009

    Remember we offer 100% refunds should you not be delighted with the performance of our antibodies.

    Tuesday, February 26, 2019

    Neuromics' Human Brain Pericytes Guide Axon Growth

    Study Interactions Between Blood Vessels and Nerve Cells
    Our GFP-Labeled Human Brian Pericytes were used by Spinal Cord Injury Researchers to evaluate the efficacy of aligned microvessels to induce and control directional axon growth from neural progenitor cells in vitro and host axons in a rat spinal cord injury model. Interstitial fluid flow aligned microvessels generated from co-cultures of cerebral-derived endothelial cells and pericytes in a three-dimensional scaffold. Paul P. Partyka, Ying Jin, Julien Bouyer, Angelica DaSilva, George A. Godsey, Robert G. Nagele, Itzhak Fischer & Peter A. Galie (2019). Harnessing neurovascular interaction to guide axon growth. Scientific Reports volume 9, Article number: 2190. https://doi.org/10.1038/s41598-019-38558-y

    Axon guidance at the site of a cervical spinal cord injury in a rat model. (Ai) Schematic illustrating transplantation of scaffold into a C-4 hemisection. The injury cavity is shown prior to (ii) and immediately following (iii) transplantation. (Bi) Scaffold conditioned with flow exhibits viable GFP-labeled microvessels (green) (ii) and alignment of host axons (magenta) infiltrating the scaffold in the rostral-caudal direction (grey arrow). (C) Scaffold conditioned in static conditions showing disrupted alignment of both microvessels (ii) and host axons (iii). (D–F) Microvessel and axon plots showing alignment (D,E) and length (F). Scale bars, 1 mm (Aii,Aiii) and 50 μm (B,C). Data are presented as mean ± s.e.m. ***P < 0.001; statistical significance was calculated using Welch Two Sample t-test. White arrows denote proximity of axons with microvessels. Microvessel alignment values (n = 30), axon alignment values (n = 30), microvessel length values (n = 15), and axon length values (n = 15) are from single hydrogel samples per condition.
    The authors conclude aligned microvessels have the dual benefit of providing the basis for a vascular bed within the scaffold to promote cell survival and directing the growth of regenerating axons. Future studies will evaluate the functional benefit resulting from delivery of this multifunctional treatment strategy in various models of CNS injury.

    Thursday, February 14, 2019

    Sorting Cancer-Associated Fibroblasts (CAFs)

    Featuring Our Colorectal CAFS
    CAFs play a central role in the Tumor Microenvironment (TME). The TME has been identified as one of the driving factors of tumor progression and invasion. Inside this microenvironment, CAFs, a type of perpetually activated fibroblasts, have been implicated to have a strong tumor modulating effect and play a key role in areas such as drug resistance.

    This makes CAFs a target for cancer therapies. The challenge is the TME is heterogeneous making it a challenge to derive homogeneously relevant populations for basic research and drug discovery. This new study uses our Colorectal CAFs to identify markers for isolating these populations. Martin Nurmik, Pit Ullmann, Fabien Rodriguez, Serge Haan, Elisabeth Letellier. In search of definitions: Cancer-associated fibroblasts and their markers. doi: 10.1002/ijc.32193.

    Images: Expression of common markers in patient-derived fibroblasts. Immunofluorescent staining of primary colon cancer fibroblasts (Neuromics, #CAF05), reveals a heterogeneous expression pattern for both αSMA/ACTA2 (abcam #ab7817, 1/200) and FAP (Santa-Cruz Biotechnology #sc-65398, 1/200), while PDGFRα (abcam #ab61219, 1/200) expression in tested cells remains relatively homogenous. Nuclei of fibroblasts were stained using DAPI (DAPI Fluoromount-G® Mounting Medium). Image is representative of three independent biological experiments.Cells were imaged using a Zeiss LSM 510 Meta laser scanning confocal microscope (Carl Zeiss, Jena, Germany) with a Plan-Apochromat 63x/1.40.


    The authors conclude when selecting for CAF populations using antibody-based methods such as FACS, it is essential that multiple surface markers are used in order to avoid any chance of introducing unintentional subtype bias. Other available surface markers such as PDGFRα/β work well here, as do more general fibroblast surface markers like Thy-1 cell surface antigen (CD90), provided that the cell population is also subjected to selection with negative markers,

    Thursday, February 07, 2019

    Neuromics Human Cells at Work

    Work Great in 3-D Assays

    Neuromics Human Primary Cells are being used with increasing frequency in 3-D Cell-Based Assays. Last month, we posted a publication referencing use of our Human Pericytes in a Blood-Brain Barrier Model.

    In this publication, our GFP-Labeled HUVECS are used in a 3-D Microfluidics Chip Model to study the impact of indoor airborne particles on human health. Yan Li, Chuanlin Hu, Pengcheng Wang, Yan Liu, Luyang Wang, Qingmeng Pi, Zhiyong Gong, Xu Yang, Michael Mak and Yang Wu (2019). Indoor nanoscale particulate matter-induced coagulation abnormality based on a human 3D microvascular model on a microfluidic chip. Journal of Nanobiotechnology 2019 17:20. https://doi.org/10.1186/s12951-019-0458-2

    Images: Development progress of human umbilical vein endothelial cells (HUVECs) by 2D culture and 3D culture. a 2D HUVEC culture in a disk, b–e camera image of fluorescent HUVECs developed from day 1 to day 4. f 3D HUVEC culture in a chip, g–j camera image of fluorescent HUVECs developed from day 1 to day 4.
    Remember, we have a 100% money back policy should the cells not work in your hands.

    Monday, January 28, 2019

    Neuromics' Islet-1 Used to Study Avian Heart Morphology

    Evolution of High-Performance Hearts

    Our Islet-1 Antibody showed its versatility a comparative morphology of avian hearts.

    Figure: Sinuatrial node in Mallard (a–c), chicken HH42 (d–g), Lesser redpoll (h–j), and Jackdaw (k–m). All sections are in the transverse plane. The boxed areas in the left-hand column images indicate the areas of the images of the middle and right-hand columns. (a–c) In the Mallard, a nodal structure at the base of the right leaflet of the sinuatrial valve (black arrowhead in [a]) expressed Isl1 (b, c) and had a large coronary artery (white arrowhead in [b]). Sections 271 (cranial) to 1201 (caudal) encompassed the atria and the sections shown are 691 (a), 692 (b), 762 (c). (d–g) In the chicken HH42, the sinus venosus (SV) expressed the myocardial marker cTnI and this expression was relatively weak at the base of the right leaflet of the sinuatrial valve (black arrowhead in [e]). (f–g) The base of the right leaflet of the sinuatrial valve expressed Bmp2 (red arrowheads in [f]) and Isl1 [g]). Sections 17 (cranial) to 297 (caudal) encompassed the atria and the sections shown are 80 (d-e), 78 (f ), 88 (g). The sections were from the mid-height of the atria. (h–j) In the lesser redpoll, Isl1 was expressed in the base of the right leaflet of the sinuatrial valve. There was no nodal structure but the Isl1 expressing wall was thicker than the surrounding walls and contained a large coronary artery (white arrowhead in [i]). Sections 321 (cranial) to 621 (caudal) encompassed the atria and the sections shown are 401 (h), 402 (i), 422 (j). (k–m) In the Jackdaw an Isl1 positive area was seen in the left sinus venosus myocardium (l) in which a coronary artery was visible (white arrowhead in (l). At the base of the right sinuatrial leaflet no positive Isl1 cells could be seen (m). Sections 122 (cranial) to 332 (caudal) encompassed the atria and the sections shown are 190 (k) and 191 (l–m). Ao = aorta; LA = left atrium; PA = pulmonary artery; RA = right atrium; SAJ = sinuatrial junction. In the picro-sirius red images blood has been painted over with white for clarity. DOI: 10.1002/jmor.20952

    We have an extensive catalog of high-performance antibodies.

    Friday, January 18, 2019

    Markers for the Study of Diabetic Neuropathy

    Widely Used and Frequently Published
    Our Pain and Inflammation Research Antibodies have proven valuable for the study of neuropathic, nociceptive and inflammatory pain.

    This recent publication is focused on Diabetic Neuropathy. José Eduardo Roa-Coria, Jorge Baruch Pineda-Farias, Paulino Barragán-Iglesias, Geovanna Nallely Quiñonez-Bastidas, Ángel Zúñiga-Romero, Juan Carlos Huerta-Cruz, Juan Gerardo Reyes-García, Francisco Javier Flores-Murrieta, Vinicio Granados-SotoView ORCID ID profile and Héctor Isaac Rocha-González (2019). Possible involvement of peripheral TRP channels in the hydrogen sulfide-induced hyperalgesia in diabetic rats. BMC Neuroscience 201920:1 https://doi.org/10.1186/s12868-018-0483-3.

    The results show Hydrogen Sulfide (H2S) catalyzes hyperalgesia in rats via TRP Channels. Our Substance P is used in the study.


    Figure: Immunolocalization of cystathionin-β-synthase enzyme (CBS, red) with a–e neuronal nuclear antigen (NeuN)-, f–j substance P (SP)-, k–o isolectin B4 (IB4)- and p–t glial fibrillary acidic protein (GFAP)-positive dorsal root ganglion neurons of diabetic rats (green). a, f, k and p show a representative 16 µm-slice from dorsal root ganglia. b, g, l and q show a representative CBS staining with Cy3 from dorsal root neurons, whereas c, h, m and r show NeuN, SP, IB4 and GFAP representative staining with Cy2 in dorsal root neurons, respectively. d, i, n and s show the merged image from Cy3 and Cy2 signals, the overlapping between CBS and neuronal markers is shown in a yellow-orange color. e, j, o and t show a magnification of d, i, n and s, respectively.


    The authors conclude that H2S leads to hyperalgesia in diabetic rats through activation of TRPV1, TRPA1 and TRPC channels and, subsequent intraepidermal fibers loss. CBS enzyme inhibitors or TRP-channel blockers could be useful for the treatment of painful diabetic neuropathy.

    Wednesday, January 09, 2019

    Rat Hippocampal Neurons

    Used to Study Apoptosis Induced by Brain Insults
    Although our primary human cells are increasingly being used in drug discovery and toxicology assays versus animal cells, we are always pleased to see the success of the latter in sophisticated studies.

    This publication references use of our e18 rat hippocampal neurons. These cells continue to be easy to culture, pure and potent. Chiara Porro, Antonia Cianciulli, Teresa Trotta, Dario Domenico Lofrumento, Rosa Calvello and Maria Antonietta Panaro. (2019). Formyl-methionyl-leucyl-phenylalanine Induces Apoptosis in Murine Neurons: Evidence for NO-Dependent Caspase-9 Activation. Biology 8(1), 4; doi:10.3390/biology8010004.

    Highlights
    Formyl-methionyl-leucyl-phenylalanine (fMLP) may be present in the brain in the course of some infectious diseases of the central nervous system (CNS), although little is known about its role. This investigation was performed to study the effect of fMLP on neuron apoptosis. The results showed that fMLP treatment of primary cultures of neurons was able to induce morphological features of apoptosis in cell cultures, as well as activation of the intrinsic apoptotic pathway, through the upregulation of caspase-9 and caspase-3.

    Figure. (A) Western blot analysis was performed on membrane-enriched cell extracts (25 µg lysate) of primary neurons. The blots were probed with formyl peptide receptor 2 (FPR2) antibody (Ab) and detected by chemiluminescence. A ~41 kDa band corresponding to FPR2 was observed as compared to the positive control. Lane 1: Marker; lane 2: Positive control, lane 3: Primary neuron lysate. (B) Immunofluorescence identification of FPR. Double staining shows the expression of the FPR receptor on the cell membrane and neurofilaments. FPR2 expression (green); skeleton protein staining of neuron-specific neurofilament 68 (red); DAPI nuclear staining (blue); cells stained by both neurofilament 68, FPR2 and DAPI (merged). Scale bar: 100 μm. 1): neurofilaments stain; 2) FPR2 expression; 3) DAPI stain; 4) merged.

    The present study emphasizing the potential role of infectious agents, such as N-formyl peptides, in neurodegenerative diseases may help to promote the development of new therapies able to modulate the expression of the N-formyl peptide receptors.

    Tuesday, January 01, 2019

    Human Primary Cells Trifecta

    Tri-culture of Neuromics' Human Primary Cells in 3-D BBB Model

    I mentioned in an earlier post that we take Researchers questioning the validity of our Human Primary Cells very seriously. We follow up with our clients to make sure our cells are working as expected. If they are not 100% happy, we offer a free replacement or full refund.

    ...So it is encouraging to see Researchers using our Human PericytesBrain Microvascular Endothelial Cells (HBMES) and Astrocytes to build a static 3-D Blood-Brain Barrier Model.

    Ece Bayir, M. Mert Celtikoglu, Aylin Sendemira. The use of bacterial cellulose as a basement membrane improves the plausibility of the static in vitro blood-brain barrier model. https://doi.org/10.1016/j.ijbiomac.2018.12.257.
    Image: Pericytes, HBMECS, and Astrocytes 5 days after culturing. Live cells (green) and dead cells (red).

    The investigators concluded, "Caffeine and sucrose permeability values obtained from all models were close to literature data and physiological values."

    Thursday, December 27, 2018

    Primary Human Brain Pericytes

    Pure and Potent
    The authors of a chapter on pericytes published in SpringerLink referred to our human primary pericytes as "pericyte-like", but chose not to characterize them because of the "exorbitant cost". Dore-Duffy P., Esen N. (2018) The Microvascular Pericyte: Approaches to Isolation, Characterization, and Cultivation. In: Birbrair A. (eds) Pericyte Biology - Novel Concepts. Advances in Experimental Medicine and Biology, vol 1109. Springer, Cham

    Given that these pericytes are also part of our hot selling 3-D Human BBB Model, we are in the process of challenging these claims. We will always take inaccurate claims regarding our solutions seriously, and we take immediate action.
    • Characterization of cells-we validated several key markers by immunofluorescence.


    Staining of Desmin (dilution 1:100). Secondary antibody conjugated to Alexa 594 (red) and counterstained with DAPI (blue). Cells were mounted using iBrite mounting media.

    Staining of Actin (dilution 25 ug/mL). Secondary antibody conjugated to Alexa 594 (red) and counterstained with DAPI (blue). Cells were mounted using iBrite mounting media.
    We also plan on doing a phenotypic analysis of these cells and will post here when completed.
    • Cost of cells-isolating pericytes from human donors, and expanding to the required number of cells involves much time and effort. We know! Further differentiating stem cells into pericytes is hard and time-consuming. Against this backdrop, we consider our pricing to be inexpensive. Though they are more difficult to derive, they are priced equivalently with our other human primary cells ($789/500,000 USD Cells).
    As always, we will post new data here.

    Saturday, December 15, 2018

    Neuromics FBS at 259 USD/500 ml.

    Only 2 Weeks Left
    We wanted to get our FBS in the hands of more cell culturing experts. Our low pricing has accelerated purchasing of this culture supplement.

    More importantly, we are pleased with the positive feedback. Here are some recent reviews.
    on BirdEye
     ★
    9 days ago
    My experience with Neuromics was very positive. I asked for a sample of FBS and after to use the FBS I received. 
    on BirdEye
     
    month ago
    I purchased FBS from Neuromics. It was shipped to Canada without any problems. It arrived safely and still frozen. Cells grow well in the product. Customer service was great and I will definitely shop here again.

    Here are the most recent publications:
    1. Fei Cao, Li-Xue Yin. (2018). miR-122 enhances sensitivity of hepatocellular carcinoma to oxaliplatin via inhibiting MDR1 by targeting Wnt/β-catenin pathway. Experimental and Molecular Pathology. https://doi.org/10.1016/j.yexmp.2018.10.009 
    2. Amélie Robert, Peirun Tian, Stephen A. Adam, Mark Kittisopikul, Khuloud Jaqaman, Robert D. Goldman, and Vladimir I. Gelfand. Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport. 26 Jun 2018https://doi.org/10.1096/fj.201800604R 
    3. Mayuri Manoj Vaidya (2018). Verification of Apoptosis in MDA-MB-231 Triple Negative Breast Cancer Cells Post NBA Photodynamic Therapy Using DNA Fragmentation Assay and Cell Death Dyes. (Doctoral Dissertation). University of Texas San Antonio
    The pricing is good through December 31, 2018.

    Tuesday, November 27, 2018

    Pain Receptors and Diabetic Neuropathy

    We Got the Markers
    Our Markers for Pain Research are widely used and frequently published. They are often referenced in publications on Diabetic nerve pain.

    Here are some of my past blog postings:
    Here's a model of the actual nerve receptors behind both intractable pain and loss of sensation.
    A: Simplified model of nociception under normal conditions. Free nerve endings transduce a painful stimulus into a neural signal, which propagates to DRG centrally and eventually synapses on a nociceptive neuron within the DH of the spinal cord.B: Proposed model of nociception under conditions of diabetic hyperalgesia and allodynia. A pain signal augmented by upregulated pronociceptive ion channels in sensory neurons is carried toward the DH, where it is further augmented by a hypoactive GABAergic system and subsequently diminished inhibition from an inhibitory interneuron. NMDAR, N-Methyl-D-aspartate receptors. https://doi.org/10.2337/dbi15-0006
    We will continue to post findings on the root causes and potential treatment for Neuropathy.

    Saturday, November 17, 2018

    Primary Human Schwann Cells

    Research Ready

    Our Schwann Cells have proven "rock solid" in the hands of our users.

    Image: Human Primary Schwann Cells in Culture

    Human Schwann Cells (HSwC) are isolated from the human spinal nerve. HSwC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HSwC are characterized by immunofluorescence with antibodies specific to S100, GFAP, and CD90

    Saturday, October 27, 2018

    Cardiomyocytes

    Pure, Potent and Research Ready
    We are recognized for our growing catalog of human primary and stem cells.

    These include hMSC Derived Cardiomyocytes.

    Human Cardiomyocytes stained with Cardiac Tryponin T (green) and counter stained with DAPI (blue).

    These cells are optimized to provide additional options for in-vitro testing of drug to drug candidate toxicities allowing researchers to rule out the ineffective and potentially toxic small molecules/compounds early in the process.

    Thursday, October 18, 2018

    TRP Antibodies

    Some of the Best Available
    Our TRP Channel Antibodies are widely used and frequently published.

    Here's some data from researchers using on of our TRPV1 Antibodies.

    Immunohistochemical expression of serum response factor in rat dorsal root ganglia. Dorsal root ganglia dissected from male Sprague-Dawley rat and imaged by confocal microscopy for (A) TRPV1 (blue), (B) AKAP (AKAP150, green), and (C) SRF (red), with colocalization represented in panel (D). White arrows indicate cells expressing all 3 proteins. Yellow line represents 50 μM in length; images are representative of 4 individual sections taken from 6 L4 to L6 DRG. (E) Venn diagram of coexpression between SRF (red circle), AKAP (AKAP150, green circle), and TRPV1 (blue circle). (F) Percentages of SRF coexpression with AKAP (AKAP150) or TRPV1 calculated from 8 randomly selected coverslips. DRG, dorsal root ganglia; SRF, serum response factor. Source Serum response factor mediates nociceptor inflammatory pain plasticity PAIN Reports3(3):e658, May/June 2018.
    We offer 100% refund or free replacements should you not be delighted with your results.

    Sunday, October 07, 2018

    Neuromics New Neuronal Markers

    Best of the Best
    We are recognized for having some of the best Neuronal-Glial Antibodies in the business. We are pleased to announce the addition of these:

    CH22122 – Tyrosine Hydroxylase
    GT22101 - GFP
    GT22102 – MAP2
    GT22103 - Vimentin
    MO22186 – Tyrosine Hydroxylase
    RA22135 – Tyrosine Hydroxylase

    Immunofluorescent analysis of rat brainstem section stained with GT22102, MAP2 dilution 1:2,000 in red, and costained with mouse mAb to MO22121, MBP dilution 1:5,000, in green. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was postfixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies.
    If you have questions on these or any of our solutions, not hesitate to contact me directly-pshuster@neuromics.com or 612-801-1007,

    Sunday, September 30, 2018

    Quality FBS-Only 259 USD/500 ml.

    First Come; First Serve
    We have an inventory of FBS of approximately 2000 bottles.  This inventory includes the lots we've been selling and using internally all year.  These have proven to be of the highest quality.
    Here’s a sampling of user feedback: 
    • After a referral from an ex-colleague, we tested a sample from a single batch and found that the product is great for cell cloning & hybridoma work, and generally for all other cell culture. We subsequently ordered several bottles of FBS. The sales person was very friendly and worked with me to make the purchase possible. The order arrived promptly. - Peter Dias, The Biomedical Research Institute of SC
    • We ordered several bottles of Fetal Bovine Serum on 2 occasions and are pleased with its performance in our cultures. The FBS arrived frozen in enough dry ice and was neatly and carefully packed. The FBS was very reasonably priced. Thank you. - Ken Patrene, University of Pittsburgh
    Referenced in Publications:
     FBS
    N39 Cell Line Cells grown in Media supplemented with Neuromics' FBS, Courtesy of Deng Guo, University of Iowa.
    FBS
    HEK Cells cultured in Media Supplemented with Neuromics' FBS Courtesy of Kavita Shah, Purdue University

    The reason for this great offer is we need to clear inventory so that we can place a volume order of raw FBS stock yet this year and lock in 2019 pricing.  Our goal is to provide the best FBS at the lowest price.  This offer of $259/500ml is valid until the current stock is exhausted.
    Like a sample to try?  Contact rose@neuromics.com for a 50 ml sample.  Rose Ludescher-Manager of Customer Satisfaction-

    Thursday, September 20, 2018

    Human Brain Microvascular Endothelial Cell in Action

    Recent Pubs
    Our Human Brain Microvascular Endothelial Cells (HBMECs) are known for the performance in drug discovery and tox assays. These 21-CFR compliant cells are used in the "blood side" of our 3-D BBB Models.

    1. Shavali Shaik, Bridget Kennis, Shinji Maegawa, Keri Schadler, Yang Yanwen, Keri Callegari, Rishi R. Lulla, Stewart Goldman, Javad Nazarian, Veena Rajaram, Jason Fangusaro, and Vidya Gopalakrishnan. (2018). REST upregulates gremlin to modulate diffuse intrinsic pontine glioma vasculature. Oncotarget. 2018 Jan 12; 9(4): 5233–5250. doi: 10.18632/oncotarget.23750 
    2. Hu et al. (2016). Epigenetic Activation of WNT5A Drives Glioblastoma Stem Cell Differentiation and Invasive Growth. Cell. 167, 1281–1295. http://dx.doi.org/10.1016/j.cell.2016.10.039,
    GREM-1 is required for tube formation in vitro (A) Q-RT-PCR analysis of GREM-1 gene expression in SU-DIPG-IV cells stably expressing either control shRNA or GREM-1-specific shRNA. Lentiviral constructs expressing two different GREM-1 shRNAs (GREM-1.1 and GREM-1.2) were used to knockdown GREM-1. Efficiency of GREM-1 knockdown was determined by Q-RT-PCR and expression was normalized to 18s RNA. Significance is as shown (**less than.01). (B-C) HUVEC or human brain microvascular endothelial cells (HBMEC) were cultured in endothelial cell medium and or conditioned medium from either control shRNA or shGREM-1.2 transfected SU-DIPG-IV cells. Tube formation in matrigel was measured after 16h and images were obtained. The ability of GREM-1 to rescue loss of tube formation upon REST knockdown was determined by addition of human-recombinant GREM-1 (rGREM-1) to conditioned media-endothelial media mix. Scale bars, 100μm. (C) Quantification of tubes in matrigel shown in Figure B (right panels). Data shown is mean +/- SD, ***p less than .001, n=3. (D) Western blot analysis to assess VEGFR2 levels in SU-DIPG-IV, -VI and –XIII cells, HUVECs and HBMECs was done using anti-VEGFR2 antibodies. Tubulin served as a loading control. (E) Western blot analysis was performed to assess AKT signaling downstream of GREM-1 interaction with its potential receptor VEGFR2 in HUVEC and HBMEC. Anti-pAKT (S473), anti-pAKT (T308), total AKT, and anti-actin were employed.
    If these cells fit your assay requirements and need more data/info, do not hesitate to contact me, Pete Shuster, CEO at pshuster@neuromics.com or cell: 612-801-1007.

    Friday, September 14, 2018

    Neuromics' BBB Model and Permeability Assays

    Crossing the Blood-Brain Barrier
    Our BBB Model is being increasingly used by Bio-Pharma for drug permeability studies. Customers include Amgen, Genentech, Boehringer Ingelheim, and Merck.

    These assays are key for making sure molecules/compounds of interest will cross the barrier into the brain and at what rate. This data, in part, add clarity on best candidates to move into in-vivo testing.
    Human Blood Brain Barrier Model 3D45002 12 well
    Human Blood Brain Barrier Model 3D45002 24 well
    Human Blood Brain Barrier Model 3D45002 6 wells
    I you have interest in using our models, Rose Ludescher, Manager of Customer Satisfaction, can provide information aligned with your assay requiremenst. rose@neuromics.com or 1-866-350-1500.