Tuesday, June 30, 2015

Save 100 USD on our hN2™ Primary Neurons

Potent, Pure and Easy to Culture

Assay data continues to roll in. I am pleased by the high lot to lot consistencies of our hN2 Primary Human Neurons resulting in data reproducibility. Here're an examples of outgrowth/tox assays: In conjunction with Molecular Devices' ImageeXpress HCI platform, hN2 differentiated neuronal cells can be used to evaluate potential toxic effects of test compounds on nuerite outgrowth through visualizing cells stained with β-lll tubulin.

Figure: Dose response curves for the effect of neurotoxic agents on neurite outgrowth. hN2™ cells were cultured in the presence of cytotoxic compounds for 72h, stained for β-lll tubulin, imaged with the ImageeXpress and analyzed for neurite outgrowth.

I am always available for product related issues and questions. I cam be reached at direct phone: 612-801-1007 and pshuster@neuromics.com. Pete Shuster, CEO and Owner, Neuromics.

Wednesday, June 24, 2015

Save 100 USD on Our New Cardiomyocytes

Potent, Pure and Easy to Culture

I am pleased to announce the addition of Human Cardiomyocytes to our Stem and Cell Based Assay Solutions.

Image: Human Cardiomyocyte culture.

These cells are optimized to provide addition options for in-vitro testing of drug to drug candidate toxicities allowing researchers to rule out the ineffective and potentially toxic small molecules/compounds early in the process.

I want to make it easy for you to buy and try. If you are not delighted with your results, I will replace free of charge or refund your purchase. Please also note the select positive feedback on our other primary and stem cells:
We recently used the hMSCs derived from Umbilical Cord Blood. Their performance was nothing less than excellent. We were highly impressed with their morphology and their doubling rate. In addition the cells respond very well to accutase and maintain their performance after passaging. We highly recommend this cell line. Rodney Nash, Ph.D, Georgia State University.
Combined Hippocampus, Cortex, and Ventricular Neurons: "I got 10 million cells total after extraction from the tissue. At Day 4 they all developed long axons. Thank you so much for the replacement." Dr. Lidia Gardner, University of Tennessee HSC.
Neuromics always provides excellent products and the best customer service. I highly recommend this company's antibodies and neuronal cultures. Kirsten Raehal, Purdue Pharma

I am always available for product related issues and questions. I cam be reached at direct phone: 612-801-1007 and pshuster@neuromics.com. Pete Shuster, CEO and Owner, Neuromics.

Monday, June 22, 2015

Voices of our Customers

Your Feedback Matters-A lot

Our follow up processes include actively engaging users of our solutions for feedback and input. This includes Rose Ludescher, Manager of Customer Satisfaction, calling or sending e-mails to each user within 3 weeks of shipment. At the core is our product rating website. If users identify products related issues, we replace them or refund their purchases.

We want to hear from you...the good, the bad and the ugly. We welcome opportunities to fix issues.

We are always pleased when customers share data with us. Here's a recent example.
Image: Mouse striatum stained with D2 cell marker Enkephalin (RA14124) in green and with neuronal marker NeuN in red courtesy of Dr Heike Rebholz of City College of New York.

We are always delighted when we discover our solutions reference in Customer Publications. We try and post all of these to our website. We also post many here in recognition of our customers' research.

For all customers that share data or testimonials, we provide gift cards. It our way of saying thank you, because your feedback matters!

Monday, June 15, 2015

Network vs Isolated Bursting in Motor Neurons

Motor Neurons and MEA
Dysregulated bursting is at the root of many motor neuron/neuromuscular junction disease. ArunA Biomedical teaming with Axion Biosystems have generated relevant bursting data from our Mouse Motor Neurons cultured on Axion-Bioystem's Maestro MEA.

Figure: Mouse Motor Neuron Network Modulation by Bicuculline-ckeck out the entire presentation to learn more: GFP+ Motor Neurons: Development and in-vitro Functional Assessment on Microelectrode Arrays
Protocol User's Guide for Culturing Motor Neuron on MEA(pdf - 679Kb)
ArunA Biomedical's/Neuromic's Mouse Motor Neurons on Axion Biosystem's MEA
Save on Mouse Motor Neuron Kits Through June 30th, 2015
NameCatalog #TypeSpeciesApplicationsSizePrice
Motor Neurons-GFP+ Quick Start Kit mMN7205.QS Primary Neurons M Cell Assays 750,000 $349
Motor Neurons-GFP+ HTS Kit mMN7205-HTS Primary Neurons M Cell Assays 4 X 750,000 $989
GDNF (Human, Mouse) PR27022-2 Protein H; M 2 ug
10 ug
AB2™ Basal Neural Medium AB27011.3 Cell Growth Media H; M Cell Assays 500 ml $69
We will continue providing you content we believe important. Should you have questions, do not hesitate to contact us. Thank you and we stand ready to serve you and your team.

Pete Shuster-CEO and Owner, Neuromics, 612-801-1007, pshuster@neuromics.com

Wednesday, June 10, 2015

MEA and Motor Neurons

Plating Densities of Motor Neurons Matter!

I wanted to share some of the tips and data shared during the ArunA Biomedical's/Axion Biosystem's Webinar on MEA and our Mouse Motor Neurons

Plating Cells on Axion's MEA
  • Surface Coating PEI-laminin for adhesion and uniform monolayer development 
  • Dotting Constrains cells to the array 
  • Requires fewer cells per well 
  • Media changes every 2-3 days
  • Variations on Cell Density
Detailed Protocol for Culturing Motor Neurons on MEA
Different Plating Densities
Plating Densities between 60,000-80,000 Optimal
At these densities there was the least variation in mean firing rates. This data shows as the density increased the cells moved towards firing in synchronicity.
Figure: Difference in Mean Motor Neurons Firing Rates vs Plating Densities

The demand for using these motor neurons for neuromuscular diseases drug discovery has been brisk and growing. Should you have question on how they would work for your unique applications, do not hesitate to contact me directly @ 612-801-1007 or pshuster@neuromics.com. Pete Shuster, CEO and Owner, Neuromics

Wednesday, June 03, 2015

Neuromics' Neuronal Cultures in Action

Drug Discovery and Tox Assay Publications

Our Primary Human, Rat and Mouse Neurons are widely used and frequently cited in publications. Here's a pub hot of the presses referencing use of our e18 Rat Cortical Neurons to study the 
EPO-like cytoprotective effects cultures of these cells: James L. Miller, Timothy J. Church, Dmitri Leonoudakis, Karen Lariosa-Willingham, Normand L. Frigon, Connie S, Tettenborn, Jeffrey R. Spencer, and Juha Punnonen. Discovery and Characterization of Nonpeptidyl Agonists of The Tissue-Protective Erythropoietin Receptor. Molecular Pharmacology. May 27, 2015 mol.115.098400

...Cells were isolated from micro-surgically dissected embryonic day 18 rat cortices that were obtained from Neuromics (Edina, MN), and cultures were prepared according to the supplier's protocol.

Image: Neuromics' Cortical Neurons @ Day 6 in Culture.

I will new and unique applications for our neurons, astroglia and neural progenitors here. If you have questions, do not hesitate to contact me @ 612-801-1007 or pshuster@neuromics.com. Pete Shuster, CEO and Owner, Neuromics