Thursday, December 27, 2018

Primary Human Brain Pericytes

Pure and Potent
The authors of a chapter on pericytes published in SpringerLink referred to our human primary pericytes as "pericyte-like", but chose not to characterize them because of the "exorbitant cost". Dore-Duffy P., Esen N. (2018) The Microvascular Pericyte: Approaches to Isolation, Characterization, and Cultivation. In: Birbrair A. (eds) Pericyte Biology - Novel Concepts. Advances in Experimental Medicine and Biology, vol 1109. Springer, Cham

Given that these pericytes are also part of our hot selling 3-D Human BBB Model, we are in the process of challenging these claims. We will always take inaccurate claims regarding our solutions seriously, and we take immediate action.
  • Characterization of cells-we validated several key markers by immunofluorescence.

Staining of Desmin (dilution 1:100). Secondary antibody conjugated to Alexa 594 (red) and counterstained with DAPI (blue). Cells were mounted using iBrite mounting media.

Staining of Actin (dilution 25 ug/mL). Secondary antibody conjugated to Alexa 594 (red) and counterstained with DAPI (blue). Cells were mounted using iBrite mounting media.
We also plan on doing a phenotypic analysis of these cells and will post here when completed.
  • Cost of cells-isolating pericytes from human donors, and expanding to the required number of cells involves much time and effort. We know! Further differentiating stem cells into pericytes is hard and time-consuming. Against this backdrop, we consider our pricing to be inexpensive. Though they are more difficult to derive, they are priced equivalently with our other human primary cells ($789/500,000 USD Cells).
As always, we will post new data here.

Saturday, December 15, 2018

Neuromics FBS at 259 USD/500 ml.

Only 2 Weeks Left
We wanted to get our FBS in the hands of more cell culturing experts. Our low pricing has accelerated purchasing of this culture supplement.

More importantly, we are pleased with the positive feedback. Here are some recent reviews.
on BirdEye
9 days ago
My experience with Neuromics was very positive. I asked for a sample of FBS and after to use the FBS I received. 
on BirdEye
month ago
I purchased FBS from Neuromics. It was shipped to Canada without any problems. It arrived safely and still frozen. Cells grow well in the product. Customer service was great and I will definitely shop here again.

Here are the most recent publications:
  1. Fei Cao, Li-Xue Yin. (2018). miR-122 enhances sensitivity of hepatocellular carcinoma to oxaliplatin via inhibiting MDR1 by targeting Wnt/β-catenin pathway. Experimental and Molecular Pathology. 
  2. Amélie Robert, Peirun Tian, Stephen A. Adam, Mark Kittisopikul, Khuloud Jaqaman, Robert D. Goldman, and Vladimir I. Gelfand. Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport. 26 Jun 2018 
  3. Mayuri Manoj Vaidya (2018). Verification of Apoptosis in MDA-MB-231 Triple Negative Breast Cancer Cells Post NBA Photodynamic Therapy Using DNA Fragmentation Assay and Cell Death Dyes. (Doctoral Dissertation). University of Texas San Antonio
The pricing is good through December 31, 2018.