FACS/Phenotyping-A constant challenge working with neural stem and progenitors cells is to determine the percentage of cells of a given phenotype within the culture. Phenotypic characterization of the entire cell population is of the utmost importance in determining the efficacy of your cell culture conditions to generate the neural or glial cell of interest. Immunocytochemistry can get you a long way to understanding the types of cells you obtained in your culture, but it is both time consuming and qualitative in nature. A far better approach is to analyze your cells by FACS. Although routinely employed within many branches of biology and medical technology, FACS is not a common technique used in Neuroscience. It is our hope that by offering the IntraCtyeTM kits, intracellular FACS analysis will be more accessible to and used more often by neuroscientists. We are currently offering several basic kits as well as a kit designed for rat neural progenitor cell phenotyping (IntraCyte-rNSC). The kit has been tested with our rat Neural Stem Cell Kit and is a powerful analytic tool to analyze the neural and glial cell within your culture.
The IntraCyte kit technology and the precision and accuracy of flow cytometry, enables you to get statistically significant, multi-parameter data at the single-cell level, on thousands of cells per sample in seconds. Even though we enjoy nothing more than looking at beautifully labeled neuronal cultures, we can do without the tedious and inaccurate counting of these cells in the dark!
We would like to thank Drs. Walter Low and Jing Xiao of the University of Minnesota for providing these excellent images from experiments using select Neuromics' Neural Stem Cell Reagents
Figure1 and Figure2 Customer Data generated using Neuromics' Neural Stem Cell Kit Catalog #: NP37100. Data Courtesy of Walter Low and Jing Xiao, University of Minnesota Neural progenitor cells were plated and maintained in Proliferation Media (Figure 1). For differentiation, cells were added to 4 well chamber slides, grown in proliferation media for 24 hours and then switched to Commitment Media with various growth factors added. Cells were cultured for 14 days and then processed by immunocytochemistry. Figure 2 shows representative data from 1 experiment using bFGF/EFG (figure 2B) for differentiation.
As a company always looking for ways to better serve our customers, we welcome any comments, information, input on products, etc. from the world of Neuroscience. We send a link out each month to 9,000 plus researchers so any information or knowledge posted will extend its reach. Thank you!
Stem Cells to Nerve Cells Small synthetic molecule initiates differentiation of adult neuronal rat brain cells to neurons 12/28/2005 - Adult neuronal stem cells have the potential to mature into functional nerve cells (neurons) and neuron support cells (astroglia). The mechanisms for this process are thus far not well understood. Res... http://www.bionity.com/news/e/51090/01065/137777