Monday, March 30, 2009

Introducing Human Neuron Kits

hN2 Human Neurons Discovery Kit-New

Energize you Research!
Neuromics has formed an alliance with Aruna Biomedical. This Alliance gives us the capabilities to bring you the reliable, robust and highly scalable hN2TMHuman Neurons Discovery Kits.
These kits are designed to reduce basic Neuroscience Research and Drug Discovery timelines. Potential applications include: cellular model studies, high content screening, developmental studies, RNAi studies and genetic manipulation.
Approximate Yield=1,000,000 healthy Neurons.

hN2 Human Neurons Discovery Kit Details

Friday, March 13, 2009

Amyloid Beta and E18 Primary Hippocampal Neurons

We would like to feature an interesting application of our E18 Rat Primary Hippocampal Neurons.

Related Publication:

Karunya K. Kandimalla1, Olenych G. Scott, Smita Fulzele1, Michael W. Davidson, Joseph F. Poduslo. Mechanism of Neuronal versus Endothelial Cell Uptake of Alzheimer's Disease Amyloid β Protein. PLoS ONE 4(2): e4627. doi:10.1371/journal.pone.0004627.

... Rat primary hippocampal (RPH) neurons were isolated from the hippocampii of 18-day-old embryonic Sprague Dawley rat brains (Neuromics, Edina, MN). The hippocampii were dispersed using a fire polished Pasteur pipette and plated on poly-D-lysine (Sigma-Aldrich, St. Louis, MO) coated glass cover slips in B-27 neurobasal medium containing 0.5 mM glutamine and 25 µM glutamate (Invitrogen, Carlsbad, CA). The neuronal cells were grown under 5% CO2 in an incubator maintained at 37°C until differentiation...

Images: A–D: Uptake of fluorescein labeled Aβ40 (F-Aβ40) and Alexa Fluor® 633 labeled transferrin (AF633-Trf), clathrin-mediated endocytosis marker, in rat primary hippocampal (RPH) neurons following 30 min incubation at 37°C. (A) F-Aβ40 uptake; (B) Uptake of AF633-Trf; (C) Superimposition of images A and B; (D) Overlay of fluorescence images on the DIC image of RPH neurons. E–G: Uptake of F-Aβ40 and AF633-Trf in RPH neurons at 4°C. (E) Uptake of F-Aβ40; (F) No significant neuronal uptake of AF633-Trf at 4°C; (G) Superimposition of images D and E on the DIC image of RPH neurons; H–J: Uptake of F-Aβ40 and AF633-Trf in RPH neurons treated with 10 mM Sodium Azide and 50 mM 2-deoxy glucose, agents that are known to deplete cellular ATP. (H) Uptake of F-Aβ40; (I) No significant cellular uptake of AF633-Trf was observed; (J) Superimposition of images H and I on the DIC image of neurons.doi:10.1371/journal.pone.0004627.g010