tag:blogger.com,1999:blog-203741492024-03-16T13:15:37.283-07:00NeuromicsNeuromics' specializes in providing reagents and methods for the Neuroscience Research Community. As a natural extension, we are growing our catalog to serve Diabetes-Obesity, Immune Response, Apotosis Detection and Cancer Researchers.Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.comBlogger617125tag:blogger.com,1999:blog-20374149.post-50852221803522116392023-03-02T09:22:00.003-08:002023-03-02T09:22:30.261-08:00Neuromics Microglia in Action<p><i> Researchers Study Migration of Neuromics Microglia in Bio-Scaffolds</i></p><p>Check out the publication-https://www.sciencedirect.com/science/article/pii/S2405844023005595.</p><div class="separator" style="clear: both; text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEirU6cjBg4gH_MvYDlDfOFzp3pjjziV3GqezuwmCjN5BSsAZMJtHAjJ9a2U43mSZkeXO2DpsLhj9WFWLWmGP3_PW00aF42l16B9Ckv9vW6odXDHk7QluyVH87POuPH9xEz1FSLvt-tFA6bKZfKIHi6TB4aaChUyRpcnpLEMq9g65v0Wxoy0cA/s731/Microglia_Migration.jpg" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="686" data-original-width="731" height="300" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEirU6cjBg4gH_MvYDlDfOFzp3pjjziV3GqezuwmCjN5BSsAZMJtHAjJ9a2U43mSZkeXO2DpsLhj9WFWLWmGP3_PW00aF42l16B9Ckv9vW6odXDHk7QluyVH87POuPH9xEz1FSLvt-tFA6bKZfKIHi6TB4aaChUyRpcnpLEMq9g65v0Wxoy0cA/s320/Microglia_Migration.jpg" width="320" /></a></div><div style="text-align: center;"><i>Images: Migration of Microglia</i></div><p>Here's a link to Neuromics' portfolio of cells for Neurocientists-https://www.neuromics.com/neurons-astrocytes-schwann-cells-microglia.</p>Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com0tag:blogger.com,1999:blog-20374149.post-87996958880548568742023-01-29T09:49:00.005-08:002023-01-29T09:51:18.591-08:00Neuromics' Astrocytes/Pericytes used to Form Organoid BBB Model<b><i>BBB Model Used to Study How Lyme neuroborreliosis (LNB) penetrates the Brian.</i></b><div><br /></div>
Yvonne Adams, Anne Skovsbo Clausen, Peter Østrup Jensen, Malin Lager, Peter Wilhelmsson, Anna J. Henningson, Per-Eric Lindgren, Daniel Faurholt-Jepsen, Helene Mens, Peter Kraiczy, Kasper Nørskov Kragh, Thomas Bjarnsholt, Andreas Kjaer, Anne-Mette Lebech, Anja R. Jensen,
<a href="https://www.sciencedirect.com/science/article/pii/S2589004222021113">3D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropism</a>,
iScience,
Volume 26, Issue 1,
2023,
105838,
ISSN 2589-0042,
https://doi.org/10.1016/j.isci.2022.105838.<div><br /></div><div class="separator" style="clear: both; text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgFFJuO5IUoMXp0KfYcTiU3DSdmlWgt5gz58gFEPlYhb_XRQo6XEPCcuxvdsSE2SxbszOH_OMX_6ByFIE5JtFGIlEfjuaj7MaXOJytSFIMXBRVMAnKygaWQGXe3LohAR4b_DsCrsP-LUmWuotNTEfioVbv3QK1GwryTkvOwIMy7cfyAy6Vmcw/s748/BBB_LNB.jpg" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="465" data-original-width="748" height="228" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgFFJuO5IUoMXp0KfYcTiU3DSdmlWgt5gz58gFEPlYhb_XRQo6XEPCcuxvdsSE2SxbszOH_OMX_6ByFIE5JtFGIlEfjuaj7MaXOJytSFIMXBRVMAnKygaWQGXe3LohAR4b_DsCrsP-LUmWuotNTEfioVbv3QK1GwryTkvOwIMy7cfyAy6Vmcw/w367-h228/BBB_LNB.jpg" width="367" /></a></div><div style="text-align: center;">Image: LNB Penetrating the BBB (green Fluorescence)</div><div style="text-align: center;"><br /></div><div><br /></div>
Researchers discovered Borrelia spp. exposure triggers a loss of tight junctions. Organoid structural integrity is significantly reduced after Borrelia spp. exposureNeuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com0tag:blogger.com,1999:blog-20374149.post-22321208094256833042022-11-17T07:09:00.000-08:002022-11-17T07:09:49.699-08:00Neuromics' Microvascular Endothelial Cells in Action<p> <b><i>Widely Used and Data Rich</i></b></p><p>Neuromics Human <a href="https://www.neuromics.com/hec02">Brain Microvascular Endothelial Cells (HBMECS</a>) are frequently used and data rich. Here are HBMECS in culture showing early microtubular formation.</p><div class="separator" style="clear: both; text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgv8keFcgyyXvcHY_hE5-4c1ZMPCqehVCTS-M1IXcZ0QZWMCmQuF0pw1gA-sioq2EHUqKQ2jMSzBjB-v4vs3N0SOgXTNNYoMEklPOmqQtBGSi0vIHDZ9x1UtUJ8UwlAAeS7L24YoQHc128nDuuDHlBevC-W1w-rTdQpnNonFOXLAkVfKr_vhQ/s296/HEC02%20Tube%20Formation%201.jpg" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="222" data-original-width="296" height="247" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgv8keFcgyyXvcHY_hE5-4c1ZMPCqehVCTS-M1IXcZ0QZWMCmQuF0pw1gA-sioq2EHUqKQ2jMSzBjB-v4vs3N0SOgXTNNYoMEklPOmqQtBGSi0vIHDZ9x1UtUJ8UwlAAeS7L24YoQHc128nDuuDHlBevC-W1w-rTdQpnNonFOXLAkVfKr_vhQ/w329-h247/HEC02%20Tube%20Formation%201.jpg" width="329" /></a></div><div class="separator" style="clear: both; text-align: center;"><i>HBMECS in culture showing early microtubular formation</i></div><div class="separator" style="clear: both; text-align: left;">They are an excellent component for building Blood-Brain Barrier (BBB) Models. Questions? E-mail Mr. David Rupp-david@neuromics.com. </div><p><br /></p>Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com0tag:blogger.com,1999:blog-20374149.post-7925837089117106372022-10-13T07:17:00.005-07:002022-10-13T07:18:04.038-07:00Cancer Associated Fibroblasts and Cell Lines<p><b><i> Wide Variety</i></b></p><p>Neuromics has a growing catalog of <a href="https://www.neuromics.com/fibroblasts-cafs-cancer-cells">CAFS and Cell Lines</a>. Here's an example of our <a href="https://www.neuromics.com/caf116">Breast CAFS.</a></p><div class="separator" style="clear: both; text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjs3ZcvLxZ6eIRb2k8y0YJO6o-fR6HYYy1CR4fkThsGDFwot_3xwTum9QCrTRrcq28Hlm6wtylp0uaTzQUwNhmH5XdRuu5mNjy74oq8J5b8CsOVZoAXuLK-NzY8DkF7Y372FiFb-hGxm92mrNXYrzJ4KdD_fB_IdZDlCtISFVyaYr_5T0VJPw/s286/Breast%20CAF%20resized.jpg" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="215" data-original-width="286" height="215" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjs3ZcvLxZ6eIRb2k8y0YJO6o-fR6HYYy1CR4fkThsGDFwot_3xwTum9QCrTRrcq28Hlm6wtylp0uaTzQUwNhmH5XdRuu5mNjy74oq8J5b8CsOVZoAXuLK-NzY8DkF7Y372FiFb-hGxm92mrNXYrzJ4KdD_fB_IdZDlCtISFVyaYr_5T0VJPw/s1600/Breast%20CAF%20resized.jpg" width="286" /></a></div><blockquote style="border: none; margin: 0px 0px 0px 40px; padding: 0px;"><p style="text-align: left;"><i>Breast CAFs in culture. Grown using Stem Cell Complete Low Serum Media (cat. CAFM02)</i></p></blockquote><p>Cells are prepared and manufactured in an <a href="https://www.neuromics.com/ittrium/reference/D8x10a86x8x1?ts=$env_vars.sysdate(yyyyMMddkkmmssSSS)$">ISO 9001:2015 certified environment</a> and ISO 5 cleanroom.</p><p>Questions? email david@neuromics.com</p>Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com0tag:blogger.com,1999:blog-20374149.post-13203223501718775062022-07-29T08:47:00.001-07:002022-07-29T08:47:06.153-07:00Neuromics' Human Neurons in Action<p><span style="background-color: #fefefe; color: #0a0a0a; font-family: "Open Sans"; font-size: 16px;">Our </span><a href="https://www.neuromics.com/hnc001" style="background-color: #fefefe; box-sizing: inherit; color: #3256a5; cursor: pointer; font-family: "Open Sans"; font-size: 16px; line-height: inherit; text-decoration-line: none;">primary human neurons (cat. HNC001)</a><span style="background-color: #fefefe; color: #0a0a0a; font-family: "Open Sans"; font-size: 16px;"> have been busy lately, continuing to become one of our most popular and versatile products. Earlier this month, Japanese researchers published </span><a href="https://www.sciencedirect.com/science/article/abs/pii/S0006291X22010385#!" style="background-color: #fefefe; box-sizing: inherit; color: #3256a5; cursor: pointer; font-family: "Open Sans"; font-size: 16px; line-height: inherit; text-decoration-line: none;">their findings</a><span style="background-color: #fefefe; color: #0a0a0a; font-family: "Open Sans"; font-size: 16px;"> when creating 3D brain-like spheroids containing our primary human neurons. Their results suggest the spheroids mimic </span><em style="background-color: #fefefe; box-sizing: inherit; color: #0a0a0a; font-family: "Open Sans"; font-size: 16px; line-height: inherit;">in vivo</em><span style="background-color: #fefefe; color: #0a0a0a; font-family: "Open Sans"; font-size: 16px;"> conditions and are suitable for use as an </span><em style="background-color: #fefefe; box-sizing: inherit; color: #0a0a0a; font-family: "Open Sans"; font-size: 16px; line-height: inherit;">in vitro</em><span style="background-color: #fefefe; color: #0a0a0a; font-family: "Open Sans"; font-size: 16px;"> neurological disease model. Check out the publication for yourself </span><a href="https://www.sciencedirect.com/science/article/abs/pii/S0006291X22010385#!" style="background-color: #fefefe; box-sizing: inherit; color: #3256a5; cursor: pointer; font-family: "Open Sans"; font-size: 16px; line-height: inherit; text-decoration-line: none;">here</a><span style="background-color: #fefefe; color: #0a0a0a; font-family: "Open Sans"; font-size: 16px;">.</span></p><p></p><div class="separator" style="clear: both; text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhfGdv0GB_kvc9x7F2xrsgY32BxS_RH106mHCJ7ivm3o_ShKzeQiwP8Az54wy6b4B0fgqbiiTiucaCx4--hGmc-wO9lZ649Aq6GNfl-h4gTDHa70GEmiMBTwiTU-wOe9zPHbTfKTn6JMXlKIP64XqLFJpqHlgj5mZMPMZYsTxtep0NrKbwX_A/s2292/Human%20Neurons.jpg" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="1640" data-original-width="2292" height="272" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhfGdv0GB_kvc9x7F2xrsgY32BxS_RH106mHCJ7ivm3o_ShKzeQiwP8Az54wy6b4B0fgqbiiTiucaCx4--hGmc-wO9lZ649Aq6GNfl-h4gTDHa70GEmiMBTwiTU-wOe9zPHbTfKTn6JMXlKIP64XqLFJpqHlgj5mZMPMZYsTxtep0NrKbwX_A/w381-h272/Human%20Neurons.jpg" width="381" /></a></div><p></p><blockquote style="border: none; margin: 0 0 0 40px; padding: 0px;"><blockquote style="border: none; margin: 0 0 0 40px; padding: 0px;"><blockquote style="border: none; margin: 0 0 0 40px; padding: 0px;"><blockquote style="border: none; margin: 0 0 0 40px; padding: 0px;"><blockquote style="border: none; margin: 0 0 0 40px; padding: 0px;"><blockquote style="border: none; margin: 0 0 0 40px; padding: 0px;"><p style="text-align: left;"><i>Human Neurons in Culture</i></p></blockquote></blockquote></blockquote></blockquote></blockquote></blockquote><p><span style="background-color: #fefefe; color: #0a0a0a; font-family: "Open Sans"; font-size: 16px;">Since the beginning of 2022, our neurons have been cited a </span><a href="https://www.neuromics.com/neurons-astrocytes-schwann-cells-microglia-pubs" style="background-color: #fefefe; box-sizing: inherit; color: #3256a5; cursor: pointer; font-family: "Open Sans"; font-size: 16px; line-height: inherit; text-decoration-line: none;">number of times</a><span style="background-color: #fefefe; color: #0a0a0a; font-family: "Open Sans"; font-size: 16px;"> spanning various research areas. They were used by researchers to suggest a link between the COVID-19 virus and Alzheimer's disease (</span><a href="https://www.neuromics.com/our-neurons-help-investigate-the-covid-19-virus" style="background-color: #fefefe; box-sizing: inherit; color: #3256a5; cursor: pointer; font-family: "Open Sans"; font-size: 16px; line-height: inherit; text-decoration-line: none;">lean more here</a><span style="background-color: #fefefe; color: #0a0a0a; font-family: "Open Sans"; font-size: 16px;">). Additionally, investigators published findings (</span><a href="https://www.neuromics.com/so-many-reagents-for-neuroscientists" style="background-color: #fefefe; box-sizing: inherit; color: #3256a5; cursor: pointer; font-family: "Open Sans"; font-size: 16px; line-height: inherit; text-decoration-line: none;">see here</a><span style="background-color: #fefefe; color: #0a0a0a; font-family: "Open Sans"; font-size: 16px;">) using the neurons to find a potential therapeutics target for neuroblastoma.</span></p><p><span style="background-color: #fefefe; color: #0a0a0a; font-family: "Open Sans"; font-size: 16px;">Questions? Do not hesitate to contact me pshuster@neuromics.com or 612-801-1007. Pete Shuster-CEO and Owner</span></p>Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com0tag:blogger.com,1999:blog-20374149.post-28538567831901806552022-04-20T07:00:00.005-07:002022-04-20T07:08:29.234-07:00Potent and Pure Fetal Bovine SerumPotent and pure FBS. Single use, triple filtered and manufactured under the strictest guidelines, Pricing starts at $245/500 ml<div class="separator" style="clear: both; text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgUzdhqJwcO1eeu9UXZGiJ4MySAu2q5UswapJAVTncZA2wwHwGdE5uj0cekDRS2MjHKphjq7s2IN3kfkCMUQaNVBH0EP5t5RWzAiR8VlC1HYkHfhkM67qLURnSE_7k24VvtFEEkEQTbiCUXy7EM92p2ZSMLizFWiyPBTiB5NkoCeC05UkwArg/s800/FBS-Bottles.jpg" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="533" data-original-width="800" height="242" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgUzdhqJwcO1eeu9UXZGiJ4MySAu2q5UswapJAVTncZA2wwHwGdE5uj0cekDRS2MjHKphjq7s2IN3kfkCMUQaNVBH0EP5t5RWzAiR8VlC1HYkHfhkM67qLURnSE_7k24VvtFEEkEQTbiCUXy7EM92p2ZSMLizFWiyPBTiB5NkoCeC05UkwArg/w364-h242/FBS-Bottles.jpg" width="364" /></a></div><br /><div>Check us out <a href="https://www.neuromics.com/fetal-bovine-serum-other-sera">Fetal Bovine Serum.</a></div>Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com0tag:blogger.com,1999:blog-20374149.post-36278372682422547042022-02-15T08:11:00.002-08:002022-02-15T08:19:36.086-08:00Introducing our Prostate Cancer Cells Lines<p><b><i> Check out the Options</i></b></p>
Neuromics is pleased to introduce many of the top human prostate cancer cell lines to our already expansive selection of cell offerings. To complement the DU 145 cells we added earlier this year, Neuromics is now offering VcaP, LNCaP, and PC-3 cells to complete our catalog of<a href="https://www.neuromics.com/fibroblasts-cafs-cancer-cells"> human prostate cancer cell lines.</a><div class="separator" style="clear: both; text-align: center;"><a href="https://blogger.googleusercontent.com/img/a/AVvXsEihQbLrQLfbzMtiicAVuMSo_a2w1UZ64CWKpqx3D0ymJzrfDYQvw6n7QcIJwmeaRGx4Iu1-x-3KmB8lZaJawciUXNHvjDgcrycKJWa1Z752cAnv1bMY0GMEHVQhPvI8eEc0PmL20NgxJGKAu6MXDU4M4QrIaLdfbqaWr5cCedSE4QUYMUCwDA=s540" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="386" data-original-width="540" height="229" src="https://blogger.googleusercontent.com/img/a/AVvXsEihQbLrQLfbzMtiicAVuMSo_a2w1UZ64CWKpqx3D0ymJzrfDYQvw6n7QcIJwmeaRGx4Iu1-x-3KmB8lZaJawciUXNHvjDgcrycKJWa1Z752cAnv1bMY0GMEHVQhPvI8eEc0PmL20NgxJGKAu6MXDU4M4QrIaLdfbqaWr5cCedSE4QUYMUCwDA=s320" width="320" /></a></div><div><div style="text-align: center;"><em style="background-color: #fefefe; box-sizing: inherit; color: #0a0a0a; font-family: "Open Sans"; font-size: 16px; line-height: inherit; text-align: left;">Image: VcaP cells in culture.</em></div></div><a href="https://www.neuromics.com/VC001">VCaP</a> cells are known for their high androgen receptor and PSA expression. Unlike most other prostate cancer cell lines, <a href="https://www.neuromics.com/LNC001">LNCaP</a> cells are highly sensitive to androgens and have a low tumorigenicity. Meanwhile, <a href="https://www.neuromics.com/PC001">PC-3</a> cells are noted for their high metastatic potential along with their insensitivity to androgens and fibroblast growth factors. Lastly, <a href="https://www.neuromics.com/DU001">DU 145 cells</a> are not hormone-sensitive and do not express PSA.Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com0tag:blogger.com,1999:blog-20374149.post-60887568997645415652022-01-25T12:05:00.001-08:002022-01-25T12:05:32.034-08:00Neuromics' Human Brian Microvascular Endothelial Cells in Action <b>Fc-saxatilin inhibits VEGF-induced permeability by regulating claudin-5 expression in<a href="• Fc-saxatilin prevents VEGF-induced permeability in human brain microvascular cells (HBMECs). • Fc-saxatilin inhibits VEGF-induced Src and Fak phosphorylation in HBMECs. • Fc-saxatilin blocks the downregulation of claudin-5 expression by VEGF in HBMECs." target="_blank"> human brain microvascular endothelial cells (HBMVECs)</a></b><div><b><br /></b></div><div>Neuromics' cells were at the heart of this study.</div>
Highlights: <div style="text-align: left;">Fc-saxatilin prevents VEGF-induced permeability in human brain microvascular cells (HBMVECs). </div><div style="text-align: left;">Fc-saxatilin inhibits VEGF-induced Src and Fak phosphorylation in HBMVECs. </div><div style="text-align: left;">Fc-saxatilin blocks the downregulation of claudin-5 expression by VEGF in HBMVECs.</div><div class="separator" style="clear: both; text-align: center;"><a href="https://blogger.googleusercontent.com/img/a/AVvXsEjzU853hw79BwMWpMivuP9FToerpc16Wp6-cUMRnDsO61kK0GhXdOo0G4iPAjvy7gTkPZLIGwu4Bne_JRgPd3xJLHx6wA8YEdg3cLaAzSF28PzZVBxCZxpWQy0kXpipXiV0O4o3vTwFf89DNZiGroKEaeVP91EM2gnT0tIKV_aFaOAqGijyUg=s350" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="248" data-original-width="350" height="227" src="https://blogger.googleusercontent.com/img/a/AVvXsEjzU853hw79BwMWpMivuP9FToerpc16Wp6-cUMRnDsO61kK0GhXdOo0G4iPAjvy7gTkPZLIGwu4Bne_JRgPd3xJLHx6wA8YEdg3cLaAzSF28PzZVBxCZxpWQy0kXpipXiV0O4o3vTwFf89DNZiGroKEaeVP91EM2gnT0tIKV_aFaOAqGijyUg=s320" width="320" /></a></div><div style="text-align: center;">Image: HMBECS in Culture</div><div style="text-align: center;"><br /></div><div style="text-align: left;">Protocol: Human brain microvascular endothelial cells (HBMVECs) were purchased from Neuromics (HEC02; Minneapolis, MN, USA). HBMVECs were maintained in ENDO-growth medium (MED001; Neuromics). </div><div style="text-align: left;"><br /></div><div style="text-align: left;">Here's a link to the full study: https://www.sciencedirect.com/science/article/pii/S0026286219301177#!</div>Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com0tag:blogger.com,1999:blog-20374149.post-37875989838140773652022-01-21T05:04:00.004-08:002022-01-21T05:04:52.514-08:00TRPV1 and Migraines<b>New Links to Insulin</b><p>Hungarian researchers used our guinea pig polyclonal <a href="https://www.neuromics.com/GP14100">TRPV1 receptor antibody (GP14100</a>) to help answer why heightened insulin levels can lead to migraines.</p><div class="separator" style="clear: both; text-align: center;"><a href="https://blogger.googleusercontent.com/img/a/AVvXsEg-QBDSjPdZvJg-A2CmzbrGLUxap-rQCF9lIIF7bYL7rPkTo7U7IfNPsFQbvcIX4Lp2SEh9RVZRIJa0ZJaWZzE9kb4fElmKptRcF_dNVSlpV25JdoU2NgtQqLPrbWuV-7lThdWqxq7rBy6IQVEDBMP9HSUo1uIX0lA9qV8zN8Bom5Hqod9Hbg=s677" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="662" data-original-width="677" height="338" src="https://blogger.googleusercontent.com/img/a/AVvXsEg-QBDSjPdZvJg-A2CmzbrGLUxap-rQCF9lIIF7bYL7rPkTo7U7IfNPsFQbvcIX4Lp2SEh9RVZRIJa0ZJaWZzE9kb4fElmKptRcF_dNVSlpV25JdoU2NgtQqLPrbWuV-7lThdWqxq7rBy6IQVEDBMP9HSUo1uIX0lA9qV8zN8Bom5Hqod9Hbg=w346-h338" width="346" /></a></div><p style="text-align: center;"><span style="font-size: x-small;">Immunohistochemistry in rat trigeminal ganglion. (A) Trigeminal ganglion neurons in the ophthalmic division immunoreactive for insulin receptor (InsR), TRPV1 receptor and CGRP. (B) Coexpression of insulin receptor (InsR) with TRPV1 receptor and/or CGRP in trigeminal ganglion neurons. Colours representing insulin receptor-, TRPV1 receptor- and CGRP-immunoreactivities and their coexpressions indicated in B applies also to A. (C) A trigeminal ganglion neuron retrogradely labeled with True blue expresses both insulin receptor (InsR) and TRPV1 receptor</span></p><b>
Conclusion-</b>the present findings indicate that insulin may activate TRPV1 receptors in the trigeminovascular system. Modified TRPV1 receptor function induced by insulin may also increase the sensitivity of both neural and vascular TRPV1 receptor for its agonists. Our data may provide a pathophysiological basis for the increased incidence of migraine in patients with hyperinsulin levels.Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com0tag:blogger.com,1999:blog-20374149.post-40811709949845020972021-12-31T08:58:00.002-08:002021-12-31T08:58:27.271-08:00About IBA LifesciencesIBA Lifesciences' proprietary Strep-tag® technology exploits one of the strongest non-covalent interactions in nature: the interaction of biotin and streptavidin. The system is based on the highly selective and easily controllable interaction between the synthetic Strep-tag®II peptide and the specially engineered streptavidin, called Strep-Tactin®, which is one of the most stable proteins known. The Strep-tag®II binds specifically to the engineered streptavidins, Strep-Tactin® and Strep-Tactin®XT, by occupying the binding pocket of the natural ligand biotin. Hence, the interaction is easily reversible by excessive addition of the competitor.
<div class="separator" style="clear: both; text-align: center;"><a href="https://blogger.googleusercontent.com/img/a/AVvXsEj2JVL6rmOR5CMFmxTMb74cJ2SHnkFNjGoVR3Hlks-BUuWdNK5O8cekPyLdeU4Zbi_kHn7ljL5J3fVK_9Wu0dwLRIMzQ-QElLLOql2AYn2k4XkaVIZsoH7Kl4vtgoY8rFOUOiwWxARsBKgj9TE2Wzs97qSXLWDGqj8ppu8y2pYAklSDEHVEHA=s448" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="448" data-original-width="400" height="400" src="https://blogger.googleusercontent.com/img/a/AVvXsEj2JVL6rmOR5CMFmxTMb74cJ2SHnkFNjGoVR3Hlks-BUuWdNK5O8cekPyLdeU4Zbi_kHn7ljL5J3fVK_9Wu0dwLRIMzQ-QElLLOql2AYn2k4XkaVIZsoH7Kl4vtgoY8rFOUOiwWxARsBKgj9TE2Wzs97qSXLWDGqj8ppu8y2pYAklSDEHVEHA=w358-h400" width="358" /></a></div><br /><div><br /></div>
Strep-tag®II consists of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys), whereas the Twin-Strep-tag® includes this motif two times in series connected by a linker and is accordingly composed of 28 amino acids. Both exhibit intrinsic, although unequal, affinity towards the streptavidin derivative Strep-Tactin® and its successor Strep-Tactin®XT: The binding affinity of Strep-tag®II to Strep-Tactin® (1µM) is nearly 100 times higher than to streptavidin. A further improvement was achieved by the development of Strep-Tactin®XT, which shares a nM affinity with the Strep-tag®II and a pM affinity with the Twin-Strep-tag®.<div class="separator" style="clear: both; text-align: center;"><a href="https://blogger.googleusercontent.com/img/a/AVvXsEioA5L4-mhPbaGbAYQunE4EZdhxR7Vfw7wW7852nVAOYw8FBi57EUwyfDC-lJMG3ewIkykaemR6shd6AdNmF2CaYma0IIx4mJ8GH7XzN1ziuWnaUWGLplU77hxY_2-PNTYFpxBL6VujpZu-AjxiAGxMMO_j7lVNVDSmBjtRsFGTir2uBCVkLA=s606" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="300" data-original-width="606" height="196" src="https://blogger.googleusercontent.com/img/a/AVvXsEioA5L4-mhPbaGbAYQunE4EZdhxR7Vfw7wW7852nVAOYw8FBi57EUwyfDC-lJMG3ewIkykaemR6shd6AdNmF2CaYma0IIx4mJ8GH7XzN1ziuWnaUWGLplU77hxY_2-PNTYFpxBL6VujpZu-AjxiAGxMMO_j7lVNVDSmBjtRsFGTir2uBCVkLA=w398-h196" width="398" /></a></div><br /><div>To Learn more watch this intro video/<br /><div><br /></div></div>Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com0tag:blogger.com,1999:blog-20374149.post-87527408360592851462021-11-24T05:38:00.000-08:002021-11-24T05:38:04.012-08:00Studying Diabetic Retinopathy?<p><b><i>Check out How Neuromic's Tools Are Used</i></b></p><p>The authors used four of Neuromics to complete this study-</p>
Sulodexide reduces glucose induced senescence in human retinal endothelial cells
<a href="https://www.nature.com/articles/s41598-021-90987-w" target="_blank">A. Gericke, K. Suminska-Jasińska & A. Bręborowicz
Scientific Reports volume 11, Article number: 11532 (2021) Cite this article</a>.<div><br /></div><div><i>Material and Methods</i><br /><div>Experiments were performed on HREC (<a href="https://www.neuromics.com/HEC09">#HEC09, Neuromics, Edina, MN, USA</a>) in in vitro culture. Cells were seeded in 75 cm<sup>2</sup> culture flasks coated with AlphaBioCoat Solution (#<a href="https://www.neuromics.com/AC001" target="_blank">AC00, Neuromics, Edina, MN, USA</a>) and were grown in Endo Growth Medium (<a href="https://www.neuromics.com/AC001">EKG001, Neuromics, Edina, MN, USA</a>) supplemented with fetal bovine serum 0.5% until they formed monolayers. Then the cells were harvested with cell detachment solution (#<a href="https://www.neuromics.com/ADF001">ADF001, Neuromics, Edina, MN, USA</a>) and seeded in quadruplicates into 25 cm<sup>2</sup> coated, as described above, culture flasks at a density of 1.5 × 10<sup>5</sup> cells/flask.</div></div><div><br /></div><div>We conclude that Sulodexide may have a beneficial effect in cases of diabetic retinopathy. It slows down hyperglycemia-dependent senescence of endothelial cells, which translates into the lower angiogenic and inflammatory impact of these cells. An important observation was that Sulodexide also has effective antiangiogenic and anti-inflammatory effects in the senescent HREC. That means that Sulodexide may be effective in retinal endothelial cells, which are already senescent. Further studies are required to explain the potential effect of Sulodexide on the endothelial glycocalyx structure and permeability of the retinal endothelial layer composed of the senescent cells to molecules with various size and electrical charge.</div><div><br /></div><div>We are pleased to see our solutions used in this important study. It could lead to drugs that slow diabetic retinopathy.</div>Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com0tag:blogger.com,1999:blog-20374149.post-18114418423968237122021-10-23T09:06:00.003-07:002021-11-11T06:15:49.420-08:00Neuromics' FBS and National Cancer Institutes<p><b><i> Best Price and Performance</i></b> </p><p>I was honored to work with Jessey at National Cancer Institute (NCI).</p><p>The were determining whether to change partners. According to Jessey, there were 24 lab PIs who would be supplied with the winner's FBS Although we proved competitive, we were not selected.</p><div class="separator" style="clear: both; text-align: center;"><a href="https://1.bp.blogspot.com/-YRkGufd3cM8/YXQxaRPa-MI/AAAAAAAAE9Y/IxyUX3z4yyk-Jhi6lW4aownbAaVNXBdmQCLcBGAsYHQ/s1019/Neuromics%2BFBS%2BPerformance%2B%2528002%2529.JPG" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="751" data-original-width="1019" height="270" src="https://1.bp.blogspot.com/-YRkGufd3cM8/YXQxaRPa-MI/AAAAAAAAE9Y/IxyUX3z4yyk-Jhi6lW4aownbAaVNXBdmQCLcBGAsYHQ/w366-h270/Neuromics%2BFBS%2BPerformance%2B%2528002%2529.JPG" width="366" /></a></div><div class="separator" style="clear: both; text-align: center;"><br /></div>The study was conducted with only premium grade FBS (most expensive). We are announcing a new Preferred FBS this Monday 10/25. It works well for supplementing define media and works great for most human primary sells. And works fabulous for human and animal cancer cells.<div><br /></div><div>Interested in learning more? Contact me at pshuster@neuromics.com or 612-801-1007. Pete Shuster CEO, Neuromics.<br /><p><br /></p><p><br /></p><p><br /></p><p><br /></p></div>Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com0tag:blogger.com,1999:blog-20374149.post-88731225268479790592021-10-06T07:04:00.001-07:002021-10-06T07:48:38.918-07:00Neuromics-3D Human Blood Brain Barrier!<p><b>Compound Penetration Study</b></p><p> </p><div class="separator" style="clear: both; text-align: center;"><a href="https://1.bp.blogspot.com/-F_qajG9Kcac/YV2sTyWJHII/AAAAAAAAE8k/mevPP5R1uyAHVAegxnhSgPWcbiXMtT24gCLcBGAsYHQ/s408/BBB_NEW.png" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="225" data-original-width="408" height="220" src="https://1.bp.blogspot.com/-F_qajG9Kcac/YV2sTyWJHII/AAAAAAAAE8k/mevPP5R1uyAHVAegxnhSgPWcbiXMtT24gCLcBGAsYHQ/w400-h220/BBB_NEW.png" width="400" /></a></div>
Classification:
Low permeability: Pe < 1.00 nm/s <div>Moderate permeability: 1.00 < Pe < 10.0 nm/s </div><div>High permeability: Pe > 10.0 nm/s </div><div>* The signal responses of Atenolol , </div><div>Sulpiride, and Cimetidine in receiver samples were undetectable.</div><div>For the convenience of calculation, </div><div>1/300 of the measured peak area ratio (PAR) of the relevant AD (A>B
Donor) </div><div>samples were used as the PAR values of Atenolol , Sulpiride, and Cimetidine </div><div>in receiver samples.</div><div>Check out the study-</div><div><span style="font-family: "Calibri",sans-serif; font-size: 11.0pt; mso-ansi-language: EN-US; mso-bidi-language: AR-SA; mso-fareast-font-family: "Times New Roman"; mso-fareast-language: EN-US;"><a href="https://www.neuromics.com/ittrium/reference/D8xf65cx8x1/BBB%20Penetration%20Data.pdf">https://www.neuromics.com/ittrium/reference/D8xf65cx8x1/BBB%20Penetration%20Data.pdf</a> </span></div>Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com0tag:blogger.com,1999:blog-20374149.post-33214306526330154032021-06-03T15:13:00.001-07:002021-06-03T15:13:43.490-07:00Neuromics' Renal Proximal Tubule Cells in Action<p><b><i>Human R<span style="font-family: "Times New Roman", serif; font-size: 11pt;">enal Proximal Tubule Cells Express </span><span style="font-family: "Times New Roman", serif; font-size: 11pt;">Klotho</span></i></b></p><p><span style="font-family: Times New Roman, serif;"><span style="font-size: 14.6667px;">Our <a href="https://www.neuromics.com/human-cells-tissue">human primary cells</a> are widely used and frequently referenced in a variety of <a href="https://www.neuromics.com/publications-using-our-human-cells-tissue">publications</a>. In this study, our <a href="https://www.neuromics.com/SC00A1-PTEC">RPT Cells</a> were used to study the impact of Klothko expression on protection of kidneys in diabetics: </span></span>Meng Xue, Feng Yang, Ying Le, Yanlin Yang, Bingsen Wang, Yijie Jia, Zongji Zheng, and Yaoming Xue. (2021). <a href="https://link.springer.com/article/10.1007/s00592-021-01736-4">Klotho Protects Against Diabetic Kidney Disease via AMPK- and ERK-Mediated Autophagy</a>. Acta Diabetologica, 32. doi: 10.1007/s00592-021-01736-4.</p><div class="separator" style="clear: both; text-align: center;"><div class="separator" style="clear: both; text-align: center;"><a href="https://lh3.googleusercontent.com/-dS3ExhA1SDo/YLlTdigePlI/AAAAAAAAE48/ZPRatxZpzOQ49k4YYMJSDuxTgBi_QSunwCLcBGAsYHQ/image.png" style="margin-left: 1em; margin-right: 1em;"><img data-original-height="392" data-original-width="476" height="330" src="https://lh3.googleusercontent.com/-dS3ExhA1SDo/YLlTdigePlI/AAAAAAAAE48/ZPRatxZpzOQ49k4YYMJSDuxTgBi_QSunwCLcBGAsYHQ/w400-h330/image.png" width="400" /></a></div><span style="text-align: left;"><span style="font-size: x-small;"><i>Klotho expression in human renal proximal tubule cells after high-glucose stimulation. (a) Levels of Klotho mRNA and (b) Klotho protein were assessed in human renal proximal tubule cells treated with low glucose and high glu- cose. (c) Morphological observations of Klotho expression
by confocal microscopy</i></span></span></div>
Klotho improved renal tubular cell autophagy via the AMPK and ERK path-
ways and played a role in renal protection. These findings provide new insight into the mechanism of Klotho and autophagy in DKD.
Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com1tag:blogger.com,1999:blog-20374149.post-7560954189183884302021-05-27T06:13:00.006-07:002021-05-27T06:13:37.212-07:00Trigeminal Neuralgia (Facial Pain)<p><b><i> Markers for Pain Research</i></b></p><p>Trigeminal Neuralgia is a progress chronic condition. Dysfunctional trigeminal signaling can lead to intense, searing facial pain. It is caused by pressure on the trigeminal nerve.</p><p>The root causes of the condition are not well understood. Our <a href="https://www.neuromics.com/pain-and-inflammation-research-antibodies-neuro">pain research markers</a> have been widely used and frequently published through the years and have been important contributors to our growth, Here's a recent pub referencing use of one our <a href="https://www.neuromics.com/ra10109">P2X3</a> Antibodies-Momoko Koizumi, Sayaka Asano, Akihiko Furukawa, Yoshinori Hayashi, Suzuro Hitomi, Ikuko Shibuta, Katsuhiko Hayashi, Fusao Kato, Koichi Iwata, and Masamichi Shinoda. (2021). P2X3 Receptor <a href="https://thejournalofheadacheandpain.biomedcentral.com/articles/10.1186/s10194-021-01244-4">Upregulation in Trigeminal Ganglion Neurons Through TNFα Production in Macrophages Contributes to Trigeminal Neuropathic Pain in Rats.</a> The Journal of Headache and Pain, 22, 31. doi: 10.1186/s10194-021-01244-4</p><div class="separator" style="clear: both; text-align: center;"><a href="https://1.bp.blogspot.com/-LjMitNl01ak/YK-YB1OBFaI/AAAAAAAAE4A/Svj-4MNRyJcDsQIhWUMXZFi2WOrmZFGMQCLcBGAsYHQ/s964/P2X3-TN.png" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="964" data-original-width="685" height="400" src="https://1.bp.blogspot.com/-LjMitNl01ak/YK-YB1OBFaI/AAAAAAAAE4A/Svj-4MNRyJcDsQIhWUMXZFi2WOrmZFGMQCLcBGAsYHQ/w284-h400/P2X3-TN.png" width="284" /></a></div><p style="text-align: center;"><i>Images: Changes in P2X3R expression in TG neurons innervating whisker pad skin and the involvement of P2X3R signaling in TG neurons in orofacial mechanical hypersensitivity on day 7 following TNC. a Photomicrograph of FG-labeled P2X3IR TG neurons following the TNC or sham procedure. Arrows indicate FG-labeled P2X3IR TG neurons. Scale bar: 100 μm. b Mean number of FG-labeled TG neurons ipsilateral to the TNC or sham procedure (n = 5 in each). c The frequency of FG-labeled P2X3-IR TG neurons ipsilateral to the TNC or sham procedure. * p < 0.05 vs. sham. (n = 5 in each, student’s t-test). d The time course of changes in the MHWT by subcutaneous A317491 or TNP-ATP administration on day 7 following the TNC or sham procedure.</i></p><p style="text-align: center;"><i><br /></i></p>
The signaling of TNFα released from the activated macrophages and infiltrated into and/or proliferated in the TG induces the upregulation of P2X3R expression in TG neurons innervating the orofacial region, resulting in orofacial mechanical allodynia following TNC. Consequently, TG neuronal P2X3R hyperexpression by enhanced TNFα signaling in the TG is a potential target for TN treatment..Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com0tag:blogger.com,1999:blog-20374149.post-86361403756314408332021-02-01T07:29:00.000-08:002021-02-01T07:29:01.532-08:00Malaria and the Blood-Brain Barrier<p><b><i> EPCR and ICAM Receptors</i></b></p><p>Neuromics <a href="https://www.neuromics.com/neurons-astrocytes-schwann-cells-microglia-pubs" target="_blank">Astrocytes and Pericytes</a> were used in this important study-Yvonne Adams, Rebecca W. Olsen, Anja Bengtsson, Nanna Dalgaard, Mykola Zdioruk, Sanghamitra Satpathi, Prativa K. Behera, Praveen K. Sahu, Sean E. Lawler, Klaus Qvortrup, Samuel C. Wassmer, and Anja T.R. Jensen. (2021). <a href="https://rupress.org/jem/article-standard/218/3/e20201266/211712/Plasmodium-falciparum-erythrocyte-membrane-protein" target="_blank">Plasmodium Falciparum Erythrocyte Membrane Protein 1 Variants Induce Cell Swelling and Disrupt the Blood–Brain Barrier in Cerebral Malaria</a>. Journal of Experimental Medicine, 218 (3). doi: 10.1084/jem.20201266.</p><div class="separator" style="clear: both; text-align: center;"><a href="https://1.bp.blogspot.com/-MLzFe0rhA_M/YBgc6BANvLI/AAAAAAAAE1Y/NXV7VzUF9Fk2aWAZg3W3lM5L-SM12msggCLcBGAsYHQ/s520/Malaria_ICAM-1.png" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="201" data-original-width="520" height="178" src="https://1.bp.blogspot.com/-MLzFe0rhA_M/YBgc6BANvLI/AAAAAAAAE1Y/NXV7VzUF9Fk2aWAZg3W3lM5L-SM12msggCLcBGAsYHQ/w459-h178/Malaria_ICAM-1.png" width="459" /></a></div><div class="separator" style="clear: both; text-align: left;">This is the first study showing malaria pericytes interacting with human endothelial cells. Please note we have the complete <a href="https://www.neuromics.com/3-d-cell-tissue-based-models-human-cells" target="_blank">BBB Model</a> that can be used for studies like these.</div><p><br /></p><p><br /></p>Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com2tag:blogger.com,1999:blog-20374149.post-28126302423514777852021-01-26T07:51:00.003-08:002021-01-26T07:51:47.924-08:00Human Schwann Cells that Work<p> <b>No Doubt!</b></p><p>Sometimes we hear our human cells did not express a certain receptor in the hands of our customers. We guarantee our cell "walk and talk" as advertised. If you are ever in doubt. we will test for expression of any receptors or cytoplasmic proteins. We also do this as part of our quality checks.</p><p>Here're, for example, is a pub showing our Schwann Cells working. Note the receptors tested here. Seung Min Shin, Francie Moehring, Brandon Itson-Zoske, Fan Fan, Cheryl L. Stucky, Quinn H. Hogan, and Hongwei Yu. (2021).<a href="https://www.biorxiv.org/content/biorxiv/early/2021/01/21/2021.01.20.427483.full.pdf" target="_blank"> Piezo2 Mechanosensitive Ion Channel is Located to Sensory Neurons and Non-Neuronal Cells in Rat Peripheral Sensory Pathway: Implications in Pain</a>. bioRxiv. doi: 10.1101/2021.01.20.427483</p><div class="separator" style="clear: both; text-align: center;"><a href="https://1.bp.blogspot.com/-547wMikLy5o/YBA2oYjQVYI/AAAAAAAAE04/_9GjbXkbqWstmrYoLBHouQyqQRtBlqkiQCLcBGAsYHQ/s819/Schwann_Cells.png" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="531" data-original-width="819" height="287" src="https://1.bp.blogspot.com/-547wMikLy5o/YBA2oYjQVYI/AAAAAAAAE04/_9GjbXkbqWstmrYoLBHouQyqQRtBlqkiQCLcBGAsYHQ/w444-h287/Schwann_Cells.png" width="444" /></a></div><div class="separator" style="clear: both; text-align: center;"><span style="text-align: left;"><i>Figure. IHC delineation of Piezo2 (PZ2) axonal component and Schwann cell expression. Representative montage images on sciatic nerve cryosections show double immunostaining (IS) of Piezo2 (red) with a selection of neuronal markers (green), including Tubb3 (A), NF200 (B), IB4 (C), and CGRP (D). Representative montage images on sciatic nerve cryosections show double- IS of Piezo2 (red) with glia markers (green), including S100 (E), p75NTR (F), GFAP (G), and MBP (H). Representative montage images on human Schwann cells (I) and isolated Schwann cells
(J) from rat sciatic nerve show double-IS of Piezo2 (red) with S100 (green). Scale bars: 50 um for all.</i></span></div><div class="separator" style="clear: both; text-align: left;">Check out our growing catalog of primary human cells today https://www.neuromics.com/human-cells-tissue. Satisfaction guaranteed or your money back.</div><div class="separator" style="clear: both; text-align: center;"><span style="text-align: left;"><i><br /></i></span></div><div class="separator" style="clear: both; text-align: center;"><span style="text-align: left;"><i><br /></i></span></div><div class="separator" style="clear: both; text-align: center;"><span style="text-align: left;"><i><br /></i></span></div><div class="separator" style="clear: both; text-align: center;"><span style="text-align: left;"><i><br /></i></span></div><div class="separator" style="clear: both; text-align: center;"><span style="text-align: left;"><i><br /></i></span></div><div class="separator" style="clear: both; text-align: center;"><span style="text-align: left;"><i><br /></i></span></div>
<span style="text-align: left;"></span>Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com2tag:blogger.com,1999:blog-20374149.post-13482767455712545252020-11-25T06:44:00.003-08:002020-11-25T06:44:16.361-08:00Cancer Associated Fibroblasts (CAFS) Traction Dynamics and Remodeling in 3D Matrices<p><b><i> Neuromics' Colorectal CAFS in Action</i></b></p><p>In a first of its kind study, the mechanical properties of our <a href="https://www.neuromics.com/fibroblasts-cafs-cancer-cells">CAFS</a> were determined. This is important because, understanding how these cell behave <i>in vivo </i>will help improve the efficacy of new therapies: Bashar Emon, Zhengwei Li, Md Saddam Hossain Joy, Umnia Doha, Farhad Kosari, and M Taher A Saif. (2020). <a href="https://www.biorxiv.org/content/10.1101/2020.09.24.311647v1.full" target="_blank">A Novel Method for Sensor-Based Quantification of Single/Multi-Cellular Traction Dynamics and Remodeling in 3D Matrices</a>. bioRxiv. doi: 10.1101/2020.09.24.311647</p><p>"Cells in vivo generate mechanical forces (traction) on surrounding 3D extra cellular matrix (ECM) and cells. Such traction and biochemical cues may remodel the matrix, e.g. increase stiffness, which in turn influences cell functions and forces. This dynamic reciprocity mediates development and tumorigenesis. Currently, there is no method available to directly quantify single cell traction and matrix remodeling in 3D. Here, we introduce a method to fulfil this long-standing need. We developed a high-resolution microfabricated sensor which hosts a 3D cell-ECM tissue formed by self-assembly. It measures cell forces and tissue-stiffness and can apply mechanical stimulation to the tissue. We measured single and multicellular force dynamics of fibroblasts (3T3), human colon (FET) and lung (A549) cancer cells and cancer associated fibroblasts (CAF05) with 1 nN resolution. Single cells show significant force fluctuations in 3D. FET/CAF co-culture system, mimicking cancer tumor microenvironment, increased tissue stiffness by 3 times within 24 hours."</p><p>Here's one of 10 videos illustrating these properties.</p><div class="separator" style="clear: both; text-align: center;"><iframe allowfullscreen='allowfullscreen' webkitallowfullscreen='webkitallowfullscreen' mozallowfullscreen='mozallowfullscreen' width='320' height='266' src='https://www.blogger.com/video.g?token=AD6v5dzk7szPWYESFRxBez3Pl374vXI1MtcFJ-tLIc0ATQIBc-cgA9y08YTf25TvBVSx2e-YlPTvcB11RtA' class='b-hbp-video b-uploaded' frameborder='0'></iframe></div><p> CAFS are widely used and frequently published. Check them out today!</p>Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com2tag:blogger.com,1999:blog-20374149.post-86602939619560915022020-10-23T09:42:00.006-07:002020-10-23T09:42:42.195-07:00Neuromics' n-Fect Delivers<p><b><i> Gene Expression Tools in Action</i></b></p><p style="background-color: #fefefe; box-sizing: inherit; color: #0a0a0a; font-family: "Open Sans"; font-size: 16px; line-height: 1.6; margin: 0px 0px 1rem; padding: 0px; text-rendering: optimizelegibility;">Neuromics has a wide offering of <a href="https://www.neuromics.com/transfection-kits" style="background-color: transparent; box-sizing: inherit; color: #3256a5; cursor: pointer; line-height: inherit; text-decoration-line: none;">transfection reagents</a> that are perfect for both <em style="box-sizing: inherit; line-height: inherit;">in vivo</em> and <em style="box-sizing: inherit; line-height: inherit;">in vitro</em> applications. We take pride in seeing our product used in research, which is certainly true of our transfection reagents (see <a href="https://www.neuromics.com/transfection-kits-pubs" style="background-color: transparent; box-sizing: inherit; color: #3256a5; cursor: pointer; line-height: inherit; text-decoration-line: none;">here</a>).</p><p style="background-color: #fefefe; box-sizing: inherit; color: #0a0a0a; font-family: "Open Sans"; font-size: 16px; line-height: 1.6; margin: 0px 0px 1rem; padding: 0px; text-rendering: optimizelegibility;">Furthermore, we'd like to highlight a recent use of our <a href="https://www.neuromics.com/NF30150" style="background-color: transparent; box-sizing: inherit; color: #3256a5; cursor: pointer; line-height: inherit; text-decoration-line: none;">n-Fect Transfection Kit (cat.# NF30150)</a> in a <a href="https://search.proquest.com/openview/868da1789a54426865e9732f21ac745f/1?pq-origsite=gscholar&cbl=18750&diss=y" style="background-color: transparent; box-sizing: inherit; color: #3256a5; cursor: pointer; line-height: inherit; text-decoration-line: none;">doctoral thesis</a> recently published. The paper looked into the neuroscience of alcohol addiction, looking towards the purinergic family receptor P2X4, which has been linked to alcohol addiction in mouse models. Using our n-Fect Transfection Kit, the molecular and cellular mechanisms linking P2X4 expression and voluntary alcohol consumption are explored. Our n-Fect reagents were used on brain slices containing the ventral tegmental area (VTA) of mice. They conclude that purinergic control of VTA neurons is a neurotransmitter system that should be further studied as a target for alcohol dependence drugs.</p><div class="separator" style="clear: both; text-align: center;"><a href="https://1.bp.blogspot.com/-P05TlkbDT0c/X5MG5-VG5rI/AAAAAAAAEy4/U5HLzbFrHgYsy8rK0__lufy8m0IvKeV6gCLcBGAsYHQ/s420/n-Fect.jpg" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="205" data-original-width="420" height="181" src="https://1.bp.blogspot.com/-P05TlkbDT0c/X5MG5-VG5rI/AAAAAAAAEy4/U5HLzbFrHgYsy8rK0__lufy8m0IvKeV6gCLcBGAsYHQ/w371-h181/n-Fect.jpg" width="371" /></a></div><p style="background-color: #fefefe; box-sizing: inherit; color: #0a0a0a; font-family: "Open Sans"; font-size: 16px; line-height: 1.6; margin: 0px 0px 1rem; padding: 0px; text-align: left; text-rendering: optimizelegibility;"><em style="box-sizing: inherit; line-height: inherit;">Image: </em><em style="box-sizing: inherit; line-height: inherit;">Examples of <a href="https://www.neuromics.com/PF30000" style="background-color: transparent; box-sizing: inherit; color: #3256a5; cursor: pointer; line-height: inherit; text-decoration-line: none;">p-Fect (Cat.# PF3000)</a> transfection in different cell types.</em></p><p style="background-color: #fefefe; box-sizing: inherit; color: #0a0a0a; font-family: "Open Sans"; font-size: 16px; line-height: 1.6; margin: 0px 0px 1rem; padding: 0px; text-align: left; text-rendering: optimizelegibility;"><span style="box-sizing: inherit; line-height: inherit;">Questions? Do not hesitate to contact Rose Ludescher, VP of customer satisfaction -rose@neuromics.com</span></p>Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com2tag:blogger.com,1999:blog-20374149.post-27317874595967014672020-09-19T06:47:00.004-07:002020-09-19T06:48:54.876-07:00Premium Virus Transport Media vs PBS<p> <b>There is a difference</b></p><p>Here's a video that illustrates the difference between CDC recommended Virus Transport Media and Sterile PBS (salt water). It answers the questions "what is in your VTM" and "why does it matter". Please consider sharing so it can find its way to those in need!</p><div class="separator" style="clear: both; text-align: center;"><iframe allowfullscreen="" class="BLOG_video_class" height="364" src="https://www.youtube.com/embed/miLruLWF12Y" width="456" youtube-src-id="miLruLWF12Y"></iframe></div><div class="separator" style="clear: both; text-align: center;">Check out our VTM-<a href="https://www.neuromics.com/virus-transport-media">https://www.neuromics.com/virus-transport-media</a></div>Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com2tag:blogger.com,1999:blog-20374149.post-37822208058928568722020-08-20T06:33:00.001-07:002020-08-20T06:35:53.545-07:00METHOD OF TREATING CENTRAL NERVOUS SYSTEM DISEASE<p><b><i> Blood-Brain Barrier (BBB) Permeability Assay</i></b></p><p>Yes, These are troubled times. With our current focus on eradicating COVID-19, we forget that many of the world's most insidious and costly diseases have limited treatments and no cures.</p><p>That is why Neuromics continues to develop its human based 3-D Cell-Based Assays. At the center if our offerings is our <a href="https://www.neuromics.com/3D45002" target="_blank">BBB Model</a>.</p><div class="separator" style="clear: both; text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj-cBsihY82N-c4KeQfIYZTwI3fTeFn9QTJ72GsRkqCpiEbE_2I8TUirmaUMv5HAF-ujNmHwyRXzopOiowzYXo9HPldUYBfws_y9vEgR4SkFv2QAsKgVhuODiXJRlv3iqudXJf15w/s450/3_D_BBB_12.png" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="211" data-original-width="450" height="188" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj-cBsihY82N-c4KeQfIYZTwI3fTeFn9QTJ72GsRkqCpiEbE_2I8TUirmaUMv5HAF-ujNmHwyRXzopOiowzYXo9HPldUYBfws_y9vEgR4SkFv2QAsKgVhuODiXJRlv3iqudXJf15w/w400-h188/3_D_BBB_12.png" width="400" /></a></div><p>Here's a recent patent application that includes a permeability assay using our model-Sookhee Bang, Jeong Kuen Song, Seung-wook Shin, Kwan Hee Lee, and Ho June Lee. (2020). <a href="http://www.freepatentsonline.com/y2020/0230218.html" target="_blank">Method of Treating Central Nervous System Disease</a>. United States Patent Application 20200230218</p><span style="font-size: small;"><b>
BBB Permeability Assay of AL04</b>
The in vitro human BBB model (Neuromics, USA) was established using co-cultures of primary Human brain endothelial cells (HBEC), Human brain pericytes (HBPC), and Human brain astrocytes (HBAC). In vitro human BBB model kit has two sides (luminal, blood/abluminal, brain) with 12 transwell inserts (polyester membrane, 0.4 um pore, diameter 12 mm, insert growth area: 1.12 cm2). HBPC were grown on the bottom side of the inserts, HBEC were monolayered on the upper side of the inserts, and HBAC were grown on the bottom of the 12-well culture plate (Neuromics, USA). In vitro BBB model was activated according to the manufacturer's instructions for 4 days. Briefly, the medium from both luminal and abluminal (lower, brain) sides of the transwell insert was changed every other day. Before the transport experiment, the abluminal side was filled with the permeability assay medium. For the permeability assay, purified AL04 or recombinant HSA (Sigma, USA) were added to the luminal side (0.3 ml) of the transwell insert to yield a final concentration of 1 or 10 uM. Incubations were performed on orbital shaker (100 rpm) at 37° C. Samples (150 μl) were collected from the abluminal side (1.2 ml) at 60, 120 and 240 min and immediately replaced with fresh permeability assay medium. The concentrations of transported AL04 or rHSA were measured by Human albumin quantitation ELISA kit (Bethyl laboratory, USA) and analyzed using the standard curve method. Permeability coefficients (Pe, cm s−1) were calculated using the equation: Pe=(VA/(A×c0))×(dQ/dt), where VA is volume of assay buffer in blood side (inside of the insert), A is the surface area of the insert (1.12 cm2), c0 the initial concentration of protein sample added into blood side, dQ/dt the concentration of transported protein sample in brain side in a defined time period. The permeability coefficients (Pe, cm s−1) for the purified AL04 or recombinant HSA were calculated as previously described (Prades, 2015; Nakagawa, 2009).</span><div><span style="font-size: small;"><br /></span></div><div>If you have interest and want to learn more, please email Rose Ludescher, VP, Customer Satisfaction-<a href="mailto:rose@neuromics.com">rose@neuromics.com</a></div><div><br /></div><div>Be safe; be healthy.</div>Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com1tag:blogger.com,1999:blog-20374149.post-56179236171805072512020-08-14T06:36:00.001-07:002020-08-14T06:36:01.175-07:00What's In Your Virus Transport Media (VTM)<iframe allowfullscreen="" frameborder="0" height="270" src="https://www.youtube.com/embed/lwzuXX4dQJA" width="480"></iframe>Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com2tag:blogger.com,1999:blog-20374149.post-89696715301302118742020-07-27T09:24:00.001-07:002020-07-27T09:24:26.092-07:00We Have FBS in Bulk Available<div dir="ltr" style="text-align: left;" trbidi="on">
<b><i>Key Component of Virus Transport Media (VTM)</i></b><br />
Neuromics is known to have some of the best <a href="https://www.neuromics.com/fetal-bovine-serum-other-sera" target="_blank">Fetal Bovine Serum (FBS) </a>for most applications. This includes being a component of <a href="https://www.neuromics.com/virus-transport-media" target="_blank">VTM</a> manufactured to the CDC's most recommended recipe.<br />
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Unlike some other suppliers, we are fully transparent as to pricing and available inventory. Check us out today.</div>
Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com1tag:blogger.com,1999:blog-20374149.post-68256385028175226902020-06-25T11:32:00.000-07:002020-06-25T11:32:38.179-07:00We Have VTM in Bulk<div dir="ltr" style="text-align: left;" trbidi="on">
<b><i>At Capacity for VTM Based COVID-19 Sample Transport Kits</i></b><br />
We are well on our way to delivering 100,000 Tubes + VTM to the state of Ohio. A big thank you to my team and friends for making this happen.<br />
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<i>5,000 Tubes+VTM Ready to Ship to Ohio</i></div>
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We do have a capacity to provide 4,000 Liters/Week of <a href="https://www.neuromics.com/VTM" target="_blank">VTM in 1 Liter Bottles.</a></div>
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If you have current or future needs do not hesitate to contact me directly @ pshuster@neuromics.com or direct phone 612-801-1007. Pete Shuster, Owner, Neuromics</div>
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Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com7tag:blogger.com,1999:blog-20374149.post-7906474932228671662020-05-16T10:21:00.001-07:002020-05-16T10:21:46.202-07:00We Have VTM and COVID19 Test Kits<div dir="ltr" style="text-align: left;" trbidi="on">
Providing Kits Coast to Coast and Points in Between<br />
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From Seattle, Washington to New York City and in between, our <a href="https://www.neuromics.com/virus-transport-medium-blog" target="_blank">Virus Transport Medium</a> has been used in COVID-19 testing. Neuromics is doing our part by helping to expand testing capacity, allowing the nation to recover from the harships associated with the pandemic.<br />
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Our VTM is manufactured using our <a href="https://www.neuromics.com/fbs002-hi" target="_blank">FBS</a> following CDC guidelines, ensuring that it is sterile and safe for testing. Additionally, each lost of VTM goes through quality control before release (<a href="https://www.neuromics.com/ittrium/reference/D8xd656x8x1/CoA.pdf" target="_blank">sample CoA</a>).<br />
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We offer our VTM in many different forms, including kits (<a href="https://www.neuromics.com/VTM-Kit" target="_blank">tubes and sterile swabs</a>) and larger quantities that can be aliquoted into tubes under a sterile hood.<br />
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If you have immediate needs please email <a href="mailto:david@neuromics.com" target="_blank">david@neuromics,com</a>, Manager, COVID-19 Testing Supplies and kits.<br />
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Neuromicshttp://www.blogger.com/profile/08144200376477478451noreply@blogger.com1