Human Brain Microvascular Endothelial Cells and BBB

Fc-saxatilin used to Modulate Blood-Brain Barrier (BBB)
Highlights
•Fc-saxatilin prevents VEGF-induced permeability in Neuromics' human brain microvascular cells (HBMECs).
 •Fc-saxatilin inhibits VEGF-induced Src and Fak phosphorylation in HBMECs.
 •Fc-saxatilin blocks the downregulation of claudin-5 expression by VEGF in HBMECS.

Hyun-Jung Choi, Na-Eun Kim, Il Kwon, Dukhwan Choi, Jayoung Kim, Ji Hoe Heo. (2019). Fc-saxatilin inhibits VEGF-induced permeability by regulating claudin-5 expression in human brain microvascular endothelial cells. Microvascular Research. DOI: https://doi.org/10.1016/j.mvr.2019.103953

Figure: Fc–saxatilin attenuates the phosphorylation of Fak induced by VEGF. A) HBMECs were treated with pretreated with vehicle (PBS, 0) or Fc-saxatilin (50, 100, or 300 ng/ml) in the basal medium (0.5% FBS) for 10 min and treated with VEGF (100 ng/ml) for 1 h. Control cells were incubated in the control medium without Fc-saxatilin and VEGF. Cell lysates were subjected to an immunoblot analysis with antibodies against either phospho-Fak, Fak, or actin, as indicated. Representative data from three separate experiments are shown. B) Bands in the immunoblots were quantified using ImageJ and normalized to actin (n = 3); *P < 0.05 compared to the vehicle only-treated control or VEGF-treated control.

These cells are also used to formulate our 3-D Blood-Brain Barrier Model.

Human Primary Endothelial Cells

Potent, Pure and Culture Ready
Looking for endothelial cells? Check out related publications and reviews.

Ravi D. on BirdEye ★★★★★
4 months ago We have ordered Brain derived Endothelial Cells or BECs. We could not find these in different companies, particularly from multiple donors. Overall, we are happy with the products.

Image: Human microvascular network formation based on microfluidic 3D HUVEC culture. a Schematic diagram of HUVECs seeding in the fibrin hydrogel. b Schematic diagram of microvascular network formation in the fibrin hydrogel. c Microscope image of HUVECs just seeding in fibrin hydrogel. d Confocal microscope image of human microvascular networks. Journal of Nanobiotechnologyvolume 17, Article number: 20 (2019)

Questions? Contact Rose Ludescher, Manager of Customer Satisfaction, rose@neuromics.com

Human Endothelial Cells and Drug Discovery

Pure, Potent and Culture Ready
Alterations of endothelial cells and the vasculature play a central role in the pathogenesis of a broad spectrum of the most dreadful of human diseases, as endothelial cells have the key function of participating in the maintenance of patent and functional capillaries. This makes them a key component for Drug Discovery.

We are proud that Biotech and Biopharma Cos. are increasingly using our cells. We believe we represent the best options. Here're some of the cells currently available:

• Human Bladder Microvascular Endothelial Cells
• Human Cardiac Microvascular Endothelial Cells
• Human Dermal Lymphatic Microvascular Endothelial Cells
• Human Dermal Microvascular Endothelial Cells
• Human Glomerular Microvascular Endothelial Cells
• Human Large Intestine Microvascular Endothelial Cells
• Human Ovarian Microvascular Endothelial Cells
• Human Pancreatic Microvascular Endothelial Cells
• Human Prostate Microvascular Endothelial Cells
• Human Retinal Microvascular Endothelial Cells
• Human Synovial Microvascular Endothelial Cells
• Human Tonsil Microvascular Endothelial Cells

Microvascular Endothelial Cells in Action

Figures: HRMECs treated with SUZ12 siRNA demonstrate increased miR-200b expression, decreased VEGF expression and decreased endothelial branching. A,B: Gene knockdown of EZH2 and SUZ12 was confirmed by qPCR. C,D: In HRMECs transfected with EZH2 siRNA in HG, miR-200b and VEGF were not significantly different from HG+control siRNA but decreased compared to NG+control siRNA. In HRMECs transfected with SUZ12 siRNA in HG, miR-200b was significantly increased with decreased levels of VEGF compared to HRMECs transfected with control siRNA, with levels similar to NG+control siRNA. E,F: Tube formation assay to measure endothelial branching. HRMECs transfected with HG+control siRNA demonstrated significantly increased branching compared to NG+control siRNA. Transfection of EZH2 siRNA did not reduce endothelial branching significantly compared to HG+control siRNA. However, transfection of SUZ12 siRNA significantly reduced endothelial branching compared to HG+control siRNA. 10.1371/journal.pone.0123987
We will keep you posted as we add more cell options.

Human Astrocytes

Cortex Derived Astrocytes

Neuromics is pleased to be offering yet another option for culturing Human Astrocytes. They can be passaged up to 10X-Only 749 USD/500,000 Cells

They are deigned to be easy to culture and grow.

 Image: Human Brain Astrocytes cultured with our AlphaBioCoat.

Check out our large offering of Neurons, Astroglia, Progenitors, Brain Endothelial Cells/Pericytes and Blood Brain Barrier (BBB) Model. Should you have questions on these or any of our offerings, I can be reached directly at 612-801-1007 or pshuster@neuromics.com. Thank you, Pete Shuster-CEO and Owner.

Ready to Use Blood Brain Barrier Model (BBB)

6, 12 and 24 Well Options

We are pleased to introduce our BBB Model. It is designed to provide more in vivo like data for your drug discovery and toxicology assays.

Our model mimic transport properties of the BBB due to the formation of tight junctions, higher expression of specific carriers, or great cell viability. The model includes brain endothelial cells with pericytes and astrocytes layered in an insert. This model improves endothelial cell polarization and enhance the formation of tight junctions, provide better endothelial cell-to-cell contact that is important for barrier development, and prevent the dilution of secreted neurotrophic factors, and these conditions collectively led to the development of an in vitro model that can truly mimic the BBB.

Diagram of BBB Model Transporters

Our Protocol includes methods for Drug Penetration Asays. 

Transport of a molecule (blue) through 3D Human BBB Model in a luminal to basal direction 

Calculation of apparent permeability (Papp in cm/min)

Apparent permeability coefficient Papp (in cm/min) based on the Fick's law can be calculated according to the following equation:

V_A: volume of abluminal chamber (cm³)

A: membrane surface area (1.12 cm²)

[C]_L: initial luminal tracer concentration (ng/ml)

[C]_A : abluminal tracer concentration (ng/ml) t : time of experiment (min)

 Our goal is to provide you better data...at a lower cost.

Solutions for Blood Brain Barrier Research

Neuromics Has the Key Components!

We are pleased to add Human Brain Microvascular Pericytes (HBMVPCs) to our solution set for Blood Brain Barrier (BBB) Researchers. These join our Human Brain Microvascular Endothelial Cells and hAstroPro Human Astrocyte+Astroglial-Neurons Co-Culturing Kits to round out our offerings of cells involved in BBB regulation.

 

Image: Human Brain Pericytes cultured with AlphaBioCoat (AC001).

Pericytes function at the BBB in at least two ways: by regulating BBB-specific gene expression patterns in endothelial cells, and by inducing polarization of astrocyte end-feet surrounding CNS blood vessels (see: http://www.nature.com/nature/journal/v468/n7323/full/nature09522.html).

Questions? Do not hesitate to call me or e-mail me-612-801-1007 or pshuster@neuromics.com). Pete Shuster CEO and Owner.