Wednesday, June 10, 2015

MEA and Motor Neurons

Plating Densities of Motor Neurons Matter!

I wanted to share some of the tips and data shared during the ArunA Biomedical's/Axion Biosystem's Webinar on MEA and our Mouse Motor Neurons

Plating Cells on Axion's MEA
  • Surface Coating PEI-laminin for adhesion and uniform monolayer development 
  • Dotting Constrains cells to the array 
  • Requires fewer cells per well 
  • Media changes every 2-3 days
  • Variations on Cell Density
Detailed Protocol for Culturing Motor Neurons on MEA
Different Plating Densities
Plating Densities between 60,000-80,000 Optimal
At these densities there was the least variation in mean firing rates. This data shows as the density increased the cells moved towards firing in synchronicity.
Figure: Difference in Mean Motor Neurons Firing Rates vs Plating Densities

The demand for using these motor neurons for neuromuscular diseases drug discovery has been brisk and growing. Should you have question on how they would work for your unique applications, do not hesitate to contact me directly @ 612-801-1007 or Pete Shuster, CEO and Owner, Neuromics

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