Network vs Isolated Bursting in Motor Neurons

Motor Neurons and MEA
Dysregulated bursting is at the root of many motor neuron/neuromuscular junction disease. ArunA Biomedical teaming with Axion Biosystems have generated relevant bursting data from our Mouse Motor Neurons cultured on Axion-Bioystem's Maestro MEA.

Figure: Mouse Motor Neuron Network Modulation by Bicuculline-ckeck out the entire presentation to learn more: GFP+ Motor Neurons: Development and in-vitro Functional Assessment on Microelectrode Arrays
Protocol User's Guide for Culturing Motor Neuron on MEA(pdf - 679Kb)

ArunA Biomedical's/Neuromic's Mouse Motor Neurons on Axion Biosystem's MEA

Save on Mouse Motor Neuron Kits Through June 30th, 2015

Name	Catalog #	Type	Species	Applications	Size	Price
Motor Neurons-GFP+ Quick Start Kit	mMN7205.QS	Primary Neurons	M	Cell Assays	750,000	$349
Motor Neurons-GFP+ HTS Kit	mMN7205-HTS	Primary Neurons	M	Cell Assays	4 X 750,000	$989
GDNF (Human, Mouse)	PR27022-2	Protein	H; M		2 ug 10 ug	$108 $205
AB2™ Basal Neural Medium	AB27011.3	Cell Growth Media	H; M	Cell Assays	500 ml	$69

We will continue providing you content we believe important. Should you have questions, do not hesitate to contact us. Thank you and we stand ready to serve you and your team.

Pete Shuster-CEO and Owner, Neuromics, 612-801-1007, pshuster@neuromics.com

MEA and Motor Neurons

Plating Densities of Motor Neurons Matter!

I wanted to share some of the tips and data shared during the ArunA Biomedical's/Axion Biosystem's Webinar on MEA and our Mouse Motor Neurons

Plating Cells on Axion's MEA

• Surface Coating PEI-laminin for adhesion and uniform monolayer development 
• Dotting Constrains cells to the array 
• Requires fewer cells per well 
• Media changes every 2-3 days
• Variations on Cell Density

Detailed Protocol for Culturing Motor Neurons on MEA

Different Plating Densities

Plating Densities between 60,000-80,000 Optimal
At these densities there was the least variation in mean firing rates. This data shows as the density increased the cells moved towards firing in synchronicity.

Figure: Difference in Mean Motor Neurons Firing Rates vs Plating Densities

The demand for using these motor neurons for neuromuscular diseases drug discovery has been brisk and growing. Should you have question on how they would work for your unique applications, do not hesitate to contact me directly @ 612-801-1007 or pshuster@neuromics.com. Pete Shuster, CEO and Owner, Neuromics