Infection of Mesenchymal Stem Cells

UCB derived huMSCs infected with RSVs

Here's cool customer data of our Human Mesenchymal Stem Cells.

Figure. RSV infects and replicates in human MSCs. Human MSCs grown on coverslips, infected with 1 MOI of RSV, and immunostained at 48 or 72 hours post-infection with a monoclonal antibody to RSV tagged with FITC (green), Evan’s blue dye (red) and DAPI (blue). Total magnification of images is 200X (inset 1000X) and scale bar is 50μm (A). RSVN transcripts detected in MSCs at 6, 12, 24 and 72 hours post infection normalized to mock (B). RSV titers (PFU/ml) isolated from the culture medium of infected and mock-infected MSCs is shown (C). Results representative of at least duplicate experiments. doi.org/10.1371/journal.pone.0163709.

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Our Customer Data program is progressing nicely. I am pleased to present the latest addition from Dr. Vimal Swarup, University of Utah. To qualify for the reward just email Rose Ludescher-rose@neuromics.com.

Hippocampalneuron-based guidance assay. Image of micro-contact patterned chondroitin sulfate proteoglycans (pre-mixed with AlexaFluor 594) purified from astrocyte conditioned media (CM). b) Immuno-stain of the spot in (a) with anti-CSPG antibody CS56. c) Overlay of Embryonic day-18 primary rat hippocampal neurons (Cat# PC35101, Neuromics Inc.) and the underlying surface containing μC patterned CM after 48 h of growth. d) Overlay of neurons and underlying surface containing μC patterned CSPGs purified from CM. e) Average neurite length on pLL and stamped regions after 48 h. f) Average neurite length on plain field surfaces of μC patterned CM, CSPG and pLL after 48 h. Results from this experiment show that neurons avoid CM or CSPGs purified from CM while elongating. Swarup et al. JACS. 2013

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