Friday, March 31, 2017

Immuno-fluorescence in 3-D

Potent Markers!

We are seeing more 3-D Cell Based Assays being used for Neuroscience Research. It is important researchers have the Neuron-Astroglial-Progenitor Markers required for staining in 3-D. This helps researchers determine the cell types present in their assays.

I have posted here published results. I would like to share the latest: Yiting Liu, Katherine S. Given, Danielle E. Harlow, Adeline M. Matschulat, Wendy B. Macklin, Jeffrey L. Bennett and Gregory P. Owens. Myelin-specific multiple sclerosis antibodies cause complement-dependent oligodendrocyte loss and demyelination. Acta Neuropathologica Communications Neuroscience of Disease 20175:25
DOI: 10.1186/s40478-017-0428-6

3D movie reconstructed by super-resolution structured illumination microscopy (SIM) imaging of live organotypic mouse cerebellar slices stained with MS#30 (red), then fixed and stained for MAG (blue) and NF-H (purple). MS#30 reactivity was on oligodendrocyte processes, including those contacting adjacent axons, and on myelinated MAG+ axons, outside of MAG layer. Scale bar: 2 μm. (MPG 90340 kb).
We will continue to post cool applications for our antibodies here.

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