Immuno-fluorescence in 3-D

Potent Markers!

We are seeing more 3-D Cell Based Assays being used for Neuroscience Research. It is important researchers have the Neuron-Astroglial-Progenitor Markers required for staining in 3-D. This helps researchers determine the cell types present in their assays.

I have posted here published results. I would like to share the latest: Yiting Liu, Katherine S. Given, Danielle E. Harlow, Adeline M. Matschulat, Wendy B. Macklin, Jeffrey L. Bennett and Gregory P. Owens. Myelin-specific multiple sclerosis antibodies cause complement-dependent oligodendrocyte loss and demyelination. Acta Neuropathologica Communications Neuroscience of Disease 20175:25
DOI: 10.1186/s40478-017-0428-6

3D movie reconstructed by super-resolution structured illumination microscopy (SIM) imaging of live organotypic mouse cerebellar slices stained with MS#30 (red), then fixed and stained for MAG (blue) and NF-H (purple). MS#30 reactivity was on oligodendrocyte processes, including those contacting adjacent axons, and on myelinated MAG+ axons, outside of MAG layer. Scale bar: 2 μm. (MPG 90340 kb).

We will continue to post cool applications for our antibodies here.

Adult Primary Neurons

Build Yourself a Stock. We now have adult primary neurons that can be passaged up to 5 times! You can now build a stock of 20-30 million cells.

Human primary neurons are isolated from brain cortical tissue. We highly recommend culturing in our human neuron growth media (cat#HNM001).
Check out our many Primary Neuron and Astrocyte options.

ECS Derived hN2 Human Primary Neurons grown in culture for 4 days and stained with our chicken polyclonal to Neurofilament light or low molecular weight chain NF-L, a marker of neurons. Many of the differentiating cells show strong cytoplasmic and clearly fibrillar staining for NF-L. Blue stain is DAPI and reveals cell nuclei of some nonneuronal cells in these cultures.
Check out related Markers!

alpha-II-spectrin/alpha-Fodrin	MO22110	Mouse IgG	H; M; R	IF; WB	100 ul	$245
Calcineurin A	MO15055	Mouse IgG	H; M; R	WB	100 ug	$255
Doublecortin/DCX	MO22113	Mouse IgG	B; H; M; P; R	IF; WB	100 ul	$245
FOX3	RA22113	Chicken IgY	H; M; R	IF; IHC; WB	100 ul	$245
MAP2 (Microtubule assoc. protein 2)	MO30000	Mouse IgG	H; M; P	IHC	50 ul	$365
Neurofilament NF-M	RA22105	Rabbit IgG	Ca; Ch; H; M; R	ICC; IHC; WB	100 ul	$275
Peripherin	CH22111	Chicken IgY	Ca; H; M; R	IHC	100 ul	$245
alpha Synuclein	MO22111	Mouse IgG	H; M; R	IF; WB	100 ul	$245
Tau /MAPT+	CH22113	Rabbit IgG	H; R	IF; WB	100 ul	$245