Wednesday, November 11, 2015

Reconstructing 3D Living Biomaterials

Hydrogel Solutions for Tissue Reconstruction and Repair

Alphabioregen/Neuromics are pleased to announce the use of our Hydrogels for reconstructing living biomaterials for regenerative biology and medicine: Suwan N. Jayasinghe,Jensen Auguste, Chris J. Scotton. Platform Technologies for Directly Reconstructing 3D Living Biomaterials. DOI: 10.1002/adma.201503001.

In this study, researchers use our  Collagen Hydrogel, Collagen Hydrogel Soft and Collagen Hydrogel Soft+  to describe  in vivo applications using a murine model to interrogate biocompatibility and cellular behavior post-transfer.

Luc macrophages (CC or BES) were then incorporated into a fl uid mixture containing one of three alternative collagen-based hydrogels, namely, Collagen Hydrogel, Collagen Hydrogel Soft, or Collagen Hydrogel Soft+ (resulting in six possible combinations of macrophages and biopolymer).

Overview of Results



Figure: Bioluminescent imaging of implanted macrophage–biopolymer mixes. IC-21-Luc macrophages (CC or BES) were mixed with biopolymer (Hydrogel, Soft, or Soft+) and subcutaneously injected into the dorsal fl anks of C57Bl/6 mice (three mice per hydrogel, with CC on the left fl ank and BES on the right). Following intraperitoneal injection of delta-luciferin, macrophage bioluminescence was detected using an IVIS Lumina II imaging system. A representative image from day 1 post-implantation (and 25 min post luciferin injection) is shown, indicating the peak detectable radiance (photons s −1 cm −2) in identically sized regions of interest. The CE results were very similar to the BES implants.


Figure:  Histological analysis of the macrophage–biopolymer implantation site (MSB staining). At day 4 post-implantation, skin was harvested from each dorsal fl ank, and processed for histology. Where possible, serial sections directly adjacent to those shown in Figure 4 were stained with a modifi ed trichrome stain. Mature fi brillar collagen within the dermis appeared dark blue, while the collagen within the hydrogel was generally lighter blue in appearance, indicating a less highly cross-linked or fi brillar form of collagen. Scale bar: 200 ┬Ám.

These Collagen Hydrogels are proving easy to use and effective in the hands of our customers. If you are looking for solutions for tissue reconstruction/repair or 3-D in vivo like cell based assays, do not hesitate to contact me @ direct phone: 612-801-1007 or pshuster@neuromics.com. Thank you, Pete Shuster, CEO and Owner, Neuromics.

1 comment:

Brown Ada said...

3D printing tech may alter the traditional production ways for rare compound-synthesis chemistry. But hope the compound produced in this way can act in human bodies with the same routes of the natural ones.