We get a lot of request for markers that will more specifically stain astrocytes, glia and microglia. Let's say, for example, you want to pinpoint these cell types in a mixed neuron-glial culture. You can now do a dual label and generate these results.
Image: Neuron-glial cell mixture cultures stained
with ALDH1L1 (red) and our monoclonal
antibody against GFAP (green). Blue is a DNA stain. ALDH1L1 stains
astrocytes and excludes from neuron cells. ALDH1L1 stains the astrocytes cell
body and processes, whereas GFAP labels the intermediate filament of the
cytoskeleton in subset of astrocytes. Astrocytes are positive for both ALDH1L1
and GFAP appear yellow. ALDH1L1 also labels many astrocytes not labeled by
GFAP, which appear as red. Inset:Blot of rat liver tissure homogenates blotted with ALDH1L1.
The antibody binds strongly a band at ~100 kDa.
Or how about these results:
Image: Mixed neuron-glial cultures stained with Mouse
Monoclonal GFAP, and Chicken Polyclonal Neurofilament-NF-L
(green). The GFAP antibody stains the network of astrocytes in these cultures,
while the NF-L antibody stains neurons and their processes. The blue channel
shows the localization of DNA. This antibody also works on formalin fixed
paraffin embedded brain tissues. Inset: Strip blot of rat spinal cord protein extract stained
with GFAP. A prominent band at about 55 kDa corresponds to the major isoform of
We will continue to post new additions to our Neuronal-Glial
Scientists grow retina cells from skin-derived stem cells - WASHINGTON - University of Wisconsin-Madison researchers have successfully grown multiple types of retina cells from two types of stem cells, giving new ho...
9 years ago