Tuesday, July 17, 2012

mGluR Antibodies in Action

Metabotropic glutamate receptors (mGluRs) are involved in normal brain function and can be perturbed in many neuropathologic conditions. This makes mGluR Markers important tools for Neuroscience Research.

Neuromics has a comphrensive catalog of these markers. They are widely used and frequently reference in pubs-Neuromics' mGluR customer publications. I get excited when a mGluR we recently manufactured is referenced in a publication: Hoon Shim, Chih-Ting Wang, Yen-Lin Chen, Viet Q. Chau, Kevin G. Fu, Jianqi Yang, A. Rory McQuiston, Rory A. Fisher, and Ching-Kang Chen. Defective Retinal Depolarizing Bipolar Cells (DBCs) in Regulators of G-protein Signaling (RGS) 7 and 11 Double Null Mice. JBC Papers in Press. Published on February 27, 2012 as Manuscript M112.345751. The latest version is at http://www.jbc.org/cgi/doi/10.1074/jbc.M112.345751...Animals were sacrificed by CO2 inhalation and the eyeballs were immediately enucleated. After removal of cornea and lens the resulting eyecups were immersionfixed in 4% paraformaldehyde in 1X PBS at room temperature for 15 minutes. This short fixation time ensured good mGluR6 and Gb5 signals at the OPL. After cryoprotection in 30% sucrose in 1X PBS, the eyecups were embedded in TBS (Richard Allan Scientific, Kalamazoo, MI), sectioned at 20μm thickness, and stained...

Images: Absence of Gb5 immunoreactivity in mGluR6-containing puncta in retinas of RGS7 and RGS11 double knockout (711dKO) mice. Retinal sections from wild-type control (WT) and 711dKO (DKO) mice stained for metabotropic glutamate receptor 6 (mGluR6, 1:200) and Gb5 (CT215, 1:250) demonstrates superimposed signals in the WT but absence of Gb5 signal in the DKO. Scale bar equals 10μm.

I will continue to post new developments reagrding this important category of Neurotransmission Research Antibodies.

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