Showing posts with label Retinal Pigment Epithelial Cells. Show all posts
Showing posts with label Retinal Pigment Epithelial Cells. Show all posts

Friday, January 17, 2020

Fetal Bovine Serum-More Data

Primary and Stem Cell Culture
This just came across our radar.
"SUPPLEMENTARY MATERIAL An eye opener in stroke: Mitochondrial dysfunction and stem cell repair in MCAO induced retinal ischemia"

We are always delighted when researchers supplement their cell culture media with our Fetal Bovine Serum (FBS).
  • RPE Cells and MSC Culture Retinal pigmented epithelium (RPE, CRL-4000; ATCC) cells were cultured in Dulbecco’s Modified Eagle Media/F-12 (DMEM/F-12, 11320033; Gibco) containing 10% fetal bovine serum (FBS; FBS001; Neuromics) and 0.01 mg/ml hygromycin B (10687010; Gibco) in incubator (37°C humidified, with 5% CO2, 95% air). 
  • MSCs were maintained with α-MEM (12561056; Gibco) supplemented with 20% FBS (FBS001; Neuromics), 1% penicillin/streptomycin (15140122; Gibco), 1% non-essential amino acids (11140050; Gibco), 1% GlutaMax-I (35050061; Gibco) in incubator (37°C humidified, with 5% CO2, 95% air).
MSCs’ mitochondria were detected in RPE cells after OGD. MSCs’ mitochondria were separately stained with Mitotracker prior to co-culture with RPE cells. Confocal images of RPE cells with DAPI (blue), β-tubulin (red), and MSCs’ mitochondria stained with Mitotracker (green). MSCs’ mitochondria were detected within the boundaries of RPE cells. Scale bar 10 µm.

We are offering our USDA Origin FBS for $299/500 ml. through the end of January.

Thursday, April 26, 2018

Pure and Potent Human Primary Retinal Pigment Endothelial Cells (HRPES)

Culture Ready
Neuromics is a recognized leader in providing researchers 21-CFR Compliant Primary Human Cells.

Here we feature our new Retinal Pigment Epithelial Cell (HRPES). These hard to find cells are reasonably priced and culture ready,
HRPES in Culture

Cells are provided at passage 3. HRPEs growth medium (contains 10% serum and growth supplements, Alpha-33) is recommended for cell culture and these cells have a minimum average population doubling capacity of 8 when cultured following the detailed protocol.

Tuesday, July 11, 2017

New Human RPES and BMSCS

Need Human Cells? Just Ask!

This is our cornerstone and we keep building on it. You asked for them. We are pleased to announce we now have Human Bone Marrow-Derived Stem Cells (BMSCs) and Retinal Pigment Epithelial Cells (RPES).
RPES in Culture.
BMSCS in Culture.
We will continue to post new cell offerings here.