Primary and Stem Cell Culture
This just came across our radar.
"SUPPLEMENTARY MATERIAL An eye opener in stroke: Mitochondrial dysfunction and stem cell repair in MCAO induced retinal ischemia"
We are always delighted when researchers supplement their cell culture media with our Fetal Bovine Serum (FBS).
This just came across our radar.
"SUPPLEMENTARY MATERIAL An eye opener in stroke: Mitochondrial dysfunction and stem cell repair in MCAO induced retinal ischemia"
We are always delighted when researchers supplement their cell culture media with our Fetal Bovine Serum (FBS).
- RPE Cells and MSC Culture Retinal pigmented epithelium (RPE, CRL-4000; ATCC) cells were cultured in Dulbecco’s Modified Eagle Media/F-12 (DMEM/F-12, 11320033; Gibco) containing 10% fetal bovine serum (FBS; FBS001; Neuromics) and 0.01 mg/ml hygromycin B (10687010; Gibco) in incubator (37°C humidified, with 5% CO2, 95% air).
- MSCs were maintained with α-MEM (12561056; Gibco) supplemented with 20% FBS (FBS001; Neuromics), 1% penicillin/streptomycin (15140122; Gibco), 1% non-essential amino acids (11140050; Gibco), 1% GlutaMax-I (35050061; Gibco) in incubator (37°C humidified, with 5% CO2, 95% air).
MSCs’ mitochondria were detected in RPE cells after OGD. MSCs’ mitochondria were separately stained with Mitotracker prior to co-culture with RPE cells. Confocal images of RPE cells with DAPI (blue), β-tubulin (red), and MSCs’ mitochondria stained with Mitotracker (green). MSCs’ mitochondria were detected within the boundaries of RPE cells. Scale bar 10 µm.
We are offering our USDA Origin FBS for $299/500 ml. through the end of January.
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