Researchers have developed a Neuronal Cell Line for the study of HSV-1 infection in humans. This line was developed by terminally differentiating human embryonic stem cells to neurons.
Our mouse monoclonal nestin antibody was used as a marker for the neural progenitor phase of this differentiation. Aldo Pourchet, Aram S. Modrek, Dimitris G. Placantonakis, Ian Mohr and Angus C. Wilson. Modeling HSV-1 Latency in Human Embryonic Stem Cell-Derived Neurons. Pathogens 2017, 6(2), 24; doi:10.3390/pathogens6020024.
Image: In vitro derivation of human neural stem cells by differentiation of the Hes5::GFP human embryonic stem cell line. (A) Schematic showing the multistep neural induction protocol. TGFβi stands for TGF-β receptor I inhibitor (B) Bright field image of human embryonic stem cell (hESC) colonies cultured on mouse embryonic fibroblasts prior to reaching confluence. (C) Bright field image of rosette NSCs derived from dissociated hESC colonies cultured in neural induction media. (D) Phase contrast and indirect immunofluorescence images of NSC cultures grown on poly-l-ornithine/laminin-coated dishes in neural stem cell media and probed with an antibody against nestin, a neural stem cell marker. Nuclei were visualized with DAPI.
We will continue to post publications referencing use of our solutions.