Wednesday, April 19, 2017

Hypoxia Induced Angiogenesis and Tumor Microenvironment

Neuromic's HUVECS Used in Study

The tumor microenvironment is essential for promoting tumor physiology, structure, function, and growth. It is important that emerging therapies eradicate both tumors and related microenvironment. This important study focuses on the formation of these microenvironments: ERICA K. SCHNETTLER. THE FUNCTIONAL ROLE OF MIR-210 IN HYPOXIA-INDUCED ANGIOGENESIS. A DISSERTATION SUBMITTED TO THE FACULTY OF THE UNIVERSITY OF MINNESOTA. FEBRUARY 2017...Primary Human umbilical vein endothelial cells (HUVEC) were purchased from Lonza (Basel, Switzerland) and Neuromics (Edina, MN)...

Figure: miR-210 sensitizes HUVECs to bFGF induced tube formation. (A)Representative images of HUVEC tube formation are shown at 10X magnification. Cells were treated with miR-210 or sc-miR, serum starved, and then plated on a Geltrex (reduced growth factor) layer in the presence or absence or bFGF to stimulate tube formation. (B) Histogram represents quantification of total tube length, number of nodes, and junctions in the tube formation assay described in A. Analysis done with ImageJ Angiogenesis Analyzer plugin.

We will continue to post our updates our primary Human Endothelial Cells as they develop.

No comments: