Wednesday, March 09, 2011

TrkB and Assembly/Maintenance of GABAergic Neurons

Publications that provide unique insight into neuro-and synptogenesis catch my attention.

This publication focuses on the mechanisms that direct inhibitory circuits. Specifically, the authors study assembly and maintenance of GABAergic inhibitory synapses between Golgi and granule cells in the mouse cerebellar cortex.

Albert I. Chen, Cindy N. Nguyen, David R. Copenhagen, Sylvia Badurek, Liliana Minichiello, Barbara Ranscht, and Louis F. Reichardt. TrkB (Tropomyosin-Related Kinase B) Controls the Assembly and Maintenance of GABAergic Synapses in the Cerebellar Cortex . The Journal of Neuroscience, 23 February 2011, 31(8): 2769-2780; doi: 10.1523/​JNEUROSCI.4991-10.2011.

Our cell adhesion marker-Contactin-1 was referenced: Immunohistochemistry 1:1,000; western blot: 1:1,000

Conclusion Highlights:  Kinase activity of TrkB is required not simply to initiate GABAergic synapse formation, but also to maintain these synapses in adulthood. We also show that the localization of Contactin-1 at the synaptic contacts between Golgi and granule cells requires TrkB suggesting that TrkB promotes synapse formation and maintenance, in part, by controlling the localization of cell adhesion molecules.

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