I can only imaging the intense pain suffered by people with scalding pain injuries.
Understanding the bio-processes behind this form of nociceptive pain is a step towards finding better analgesics. In this study, the authors study several key markers for pain...phosphoERK1/2 and TRPV1 (surprisingly not a major piece of the this pain signaling process).
John P.M. Whitea, Chin Wing Koa, Antonio Rei Fidalgoa, Mario Cibellia, Cleoper C. Paulea, Peter J. Andersona, Celia Cruzb, Szabolcs Gombad, Klara Matesze, Gabor Veressd, Antonio Avelinob and Istvan Nagya. Severe burn injury induces a characteristic activation of extracellular signal-regulated kinase 1/2 in spinal dorsal horn neurons. European Journal of Pain.doi:10.1016/j.ejpain.2010.12.006...pERK1/2 (1:1000, Neuromics; RA15002)..
Abstract: We have studied scalding-type burn injury-induced activation of extracellular signal-regulated kinase 1/2 (ERK1/2) in the spinal dorsal horn, which is a recognised marker for spinal nociceptive processing. At 5 min after severe scalding injury to mouse hind-paw, a substantial number of phosphorylated ERK1/2 (pERK1/2) immunopositive neurons were found in the ipsilateral dorsal horn. At 1 h post-injury, the number of pERK1/2-labelled neurons remained substantially the same. However, at 3 h post-injury, a further increase in the number of labelled neurons was found on the ipsilateral side, while a remarkable increase in the number of labelled neurons on the contralateral side resulted in there being no significant difference between the extent of the labelling on both sides. By 6 h post-injury, the number of labelled neurons was reduced on both sides without there being significant difference between the two sides. A similar pattern of severe scalding injury-induced activation of ERK1/2 in spinal dorsal horn neurons over the same time-course was found in mice which lacked the transient receptor potential type 1 receptor (TRPV1) except that the extent to which ERK1/2 was activated in the ipsilateral dorsal horn at 5 min post-injury was significantly greater in wild-type animals when compared to TRPV1 null animals. This difference in activation of ERK1/2 in spinal dorsal horn neurons was abolished within 1 h after injury, demonstrating that TRPV1 is not essential for the maintenance of ongoing spinal nociceptive processing in inflammatory pain conditions in mouse resulting from at least certain types of severe burn injury.
Scientists grow retina cells from skin-derived stem cells - WASHINGTON - University of Wisconsin-Madison researchers have successfully grown multiple types of retina cells from two types of stem cells, giving new ho...
9 years ago