Monday, February 07, 2011

More on DOR

I am always delighted when we recieve positive feedback on one of our new reagents. It is even better when a researcher graciously shares images. I would like to thank Dr. Prof. Dr. Rapheal Sell of Freie Universität Berlin for sharing these excellent Immunofluorescence images:

Images: IF detection of delta-opioid receptors in tissue engineered human oral mucosa models using Delta Opioid Receptor 361-372. The tissues were frozen at -80°C, cut into 8 µm slices and fixed with formaldehyde solution. Blocking was performed with goat serum (dilutet 1:20 in PBS) for 30 minutes at RT. Primary antibody dilutions in PBS/BSA/Tween-20 were 1:500, 1:1000 and 1:1500, slides were incubated overnight at 4°C. Secondary (fluorescent) antibody was diluted 1:400, slides were incubated for 30 minutes at room temperature in the dark. Sections were mounted with antifading mounting medium DAPI (blue) and evaluated by fluorescent microscopy

Related Reagents:
Delta Opioid Receptor 358-372
All Opioid Receptor Antibodies          
Neurotransmission Research Antibodies-GPCRs, Ligand Gated Ion Channels, Biogenic Amines and more
Primary Neurons and Astrocytes-Primary human, rat and mouse neurons and astrocytes

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