Saturday, January 15, 2011

Upregulations of P2X3 and ASIC3 involve in hyperalgesia

It gets my attention when several of our ion channel markers are referenced in the title of a publication.

Kiyomi Hori, Noriyuki Ozaki, Shigeyuki Suzuki, Yasuo Sugiura. Upregulations of P2X3 and ASIC3 involve in hyperalgesia induced by cisplatin administration in rats. PAIN 149 (2010) 393–405

Findings: "We explored the role of ion channels expressed in DRG neurons in the painful neuropathy associated with cisplatin administration. Upregulations of TRPV2, P2X3 and ASIC3 may play important roles in the mechanical hyperalgesia induced by cisplatin. In addition to cutaneous hyperalgesia, cisplatin treatment might also induce muscle hyperalgesia associated with upregulations of P2X3 and
ASIC3. Interfering with these channels may prove to be a promising therapeutic target for treating painful symptoms of cisplatin neuropathy, and may further be able to ensure the continuation of anticancer therapy."

Images: ASIC3 (Dilution 1:10) amd P2X3 (Dilution 1:500) satining of rat Dorsal Root Ganglia (DRGs) of cisplatin-treated animals. After dilution in 0.1 M phosphate-buffered saline (PBS) containing 1.5% normal goat serum and 0.3% Triton X-100 (Sigma), DRG sections were incubated with either guinea pig polyclonal antiserum against synthetic rat ASIC3 and rabbit polyclonal antiserum against synthetic rat P2X3 (1:500; Neuromics). The sections for ASIC3 were reacted with reagents for 2 days at room temperature and others at 4OC. After being rinsed with 0.1 M PBS, the sections were reacted in PBS with fluorescein-isothiocyanate (FITC)-conjugated goat anti-guinea pig or - rabbit IgG antibody (Vector Laboratories, Burlingame, CA, USA) at a concentration of 1:100. After being rinsed with 0.1 M PBS, the sections were cover-slipped in mounting medium (Immunon, Pittsburgh, PA, USA) and examined under a fluorescence microscope equipped with a digital camera

7 comments:

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Pete Shuster said...
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Pete Shuster said...

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