Friday, March 26, 2010

Potent Neuron-Glial Markers

We are recognized for having top shelf Neuron/Glial Marker Antibodies. We have an extensive catalog and have customer referencing use of these in a variety of applications, species and cell types.

Cell types include neural progenitors, neurons, glia, astrocytes, schwann cells and more. We are pleased to provide present a new publication referencing use of our MAP2 (Microtubule assoc. protein 2) Antibody for immunostaining of E17 primary mouse astrocytes.

Shelley Jacobs and Laurie C. Doering. Astrocytes Prevent Abnormal Neuronal Development in the Fragile X Mouse
. J. Neurosci., Mar 2010; 30: 4508 - 4514 ; doi:10.1523/JNEUROSCI.5027-09.2010.

After 7 d in vitro (DIV), the cells were fixed with ice-cold (–20°C) methanol and processed for immunocytochemistry. After the appropriate serum block, the cells were incubated with primary antibodies overnight at 4°C. Secondary antibodies were applied for 3 h at room temperature. The following antibody, diluted in 1% BSA, was used: chicken microtubule-associated protein 2 (MAP2) (1:20,000; Neuromics) and anti-chicken FITC (1:100; Jackson ImmunoResearch Laboratories). Coverslips were mounted with Vectashield fluorescent mounting medium with 4`,6-diamidino-2-phenylindole (DAPI).

Images: Effects of astrocytes on the growth of hippocampal neurons in coculture at 7 DIV. E17 primary hippocampal neurons were cocultured with P0–P1 primary cortical astrocytes for 7 DIV in each of four coculture conditions. a, Immunofluorescent images of neurons in each of the four culture combinations. Neurons are stained with an antibody directed against the neuronal dendritic marker, MAP2. Scale bar, 100 ┬Ám. b, Quantification of percentage of surviving neurons at 7 DIV in each of the four culture conditions. Data shown are mean values ± SEM from two or three independent experiments (10–15 regions of 1.5 mm2 from 2 coverslips per experiment). Significant differences revealed by post hoc Tukey's tests are indicated (p less than 0.001).

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