Friday, February 19, 2010

Making Gains on Pain Research

Neuromics' Pain Research Customers continue to make gains using our Pain and Inflammation Research Antibodies and Transfection Kits. Here are the latest pubs:

Hua Zhang and A. S. Verkman. Aquaporin-1 Tunes Pain Perception by Interaction with Nav1.8 Na+ Channels in Dorsal Root Ganglion Neurons. February 19, 2010 The Journal of Biological Chemistry, 285, 5896-5906.

...chicken anti-calcitonin gene-related peptide (CGRP; 1:500, Neuromics, Edina, MN)...

Nathaniel A. Sowa, Bonnie Taylor-Blake, and Mark J. Zylka. Ecto-5'-Nucleotidase (CD73) Inhibits Nociception by Hydrolyzing AMP to Adenosine in Nociceptive Circuits. The Journal of Neuroscience, February 10, 2010, 30(6):2235-2244; doi:10.1523/JNEUROSCI.5324-09.2010.

...rabbit anti-P2X3-RA10109, Neuromics; 1:750), rabbit anti- VR1 C-Terminus (TRPV1) - mouse specific (RA14113, Neuromics; 1:750

Images: Images: Confocal images showing the effect of RTX on mu opioid receptor and TRPV1 immunoreactive DRG neurons and afferent terminals in the spinal cord. A: representative confocal images showing mu opioid receptor (green) and TRPV1 (red) immunoreactivities in DRG neurons of one vehicle- and one RTX-treated rat. Scale bar, 40 um. B: confocal images showing mu opioid receptor (green) and TRPV1 (red) immunoreactivities in afferent terminals in the spinal dorsal horn of 1 vehicle- and 1 RTX-treated rat. Scale bar, 80 um. Inset: high-magnification images (scale bar = 5 um) showing co-localization of mu opioid receptor and TRPV1 immunoreactivity in the lamina I. Co-localization of the mu opioid receptor and TRPV1 immunoreactivity is indicated in yellow when 2 images are digitally merged. All images are single confocal optical sections. Shao-Rui Chen and Hui-Lin Pan. Loss of TRPV1-Expressing Sensory Neurons Reduces Spinal mu-Opioid Receptors But Paradoxically Potentiates Opioid Analgesia. doi:10.1152/jn.01343.2005.

Neuromics' i-Fect ™ siRNA Transfection Reagent continues to be used a tool for studying expression of genes suspected to paly a role in pain . Expression studies include: DOR , The β3 subunit of the Na+,K+-ATPase, rSNSR1, NTS1. NAV1.8, CaV1.2 and more.

Here's a link to all transfection publications: Transfection Kit Pubs

Related Reagents:

Neurotransmission Research Antibodies-GPCRs, Ligand Gated Ion Channels, Biogenic Amines and more
Purinergic Receptors

Primary Neurons and Astrocytes- Primary human, rat and mouse neurons and astrocytes by Category

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