Showing posts with label Nucleostemin. Show all posts
Showing posts with label Nucleostemin. Show all posts

Friday, May 29, 2015

Neuromics' Stem Cell Markers

Potent, Proven and Published

We have a growing parade of Publications referencing use of our Stem Cell Markers. They have proven to be excellent for assays using our hNP1™ Human Neural Progenitor Kits and Human Mesenchymal Stem Cells (hMSCs),

Here's a recent reference highlighting use of our Nucleostemin Antibody: Zhe Ding and He Huang. Mesenchymal stem cells in rabbit meniscus and bone marrow exhibit a similar feature but a heterogeneous multi-differentiation potential: superiority of meniscus as a cell source for meniscus repair. BMC Musculoskeletal Disorders (2015) 16:65 DOI 10.1186/s12891-015-0511-8. ...After removing the medium, the cells were washed with PBS once. MMSCs and BMSCs were fixed with 4% paraformaldehyde in PBS for 30 min at room temperature and treated with 0.1% Triton X-100 for 30 min for Nanog and nucleostemin staining. After washing the cells with PBS, either mouse anti-Nanog (1:350, Santa Cruz Biotechnology, Inc., cat. # SC-33759, Santa Cruz, CA) or goat antinucleostemin (1:400, Neuromics, Cat. # GT15050, Edina, MN)...

Figure: Expression of Nucleostemin for BMSCs (E) and MMSCs (F).
I will continue to post Stem Cell Marker success stories here.

Wednesday, January 27, 2010

Nucleostemin-Stem Cell Marker

Our Nucleostemin Antibody has proven a versatile Stem Cell Marker.

Researchers have referenced use of this antibody as a marker for Neural Progenitors and Muscle-Derived Stem Cells. In the most recent of our Nucleostemin Publications, Dr James Wang (University of Pittsburgh) references using the antibody for staining Tendon Progenitors/Stem Cells (TSCs):

Jianying Zhang and James H-C. Wang. Characterization of differential properties of rabbit tendon stem cells and tenocytes. BMC Musculoskeletal Disorders. 2010, 11:10doi:10.1186/1471-2474-11-10.
...The staining protocol used goat anti-human Nucleostemin Antibody (1:300; Neuromics, Cat. No. GT15050) and Cy3-conjugated donkey anti-goat IgG secondary antibody...

Image: Achilles Tendon Stem Cells (ATSCs) expressed nucleostemin. Insets show enlarged view of expressed nucleostemin in pink (bar: 50 μm).

Related Reagents:
Stem Cell Markers
All Stem Cell Research Reagents
Primary Neurons and Astrocytes-Primary human, rat and mouse neurons and astrocytes

Wednesday, October 01, 2008