Tuesday, January 19, 2010

i-Brite Plus!

We are all for reagents that brighten your day.

i-BRITE Plus is a glycerol based liquid. It can be used to stain cells and can be easily added to wells. It also will not shrink tissue. In addition standard to IHC/IF applications, it can be used to visualize GFP transfections and more.

Here's a publication referencing it: Ajay S. Yekkirala, Alexander E. Kalyuzhny and Philip S. Portoghese. Standard Opioid Agonists Activate Heteromeric Opioid Receptors: Evidence for Morphine and [d-Ala2-MePhe4-Glyol5]Enkephalin as Selective μ−δ Agonists. ACS Chem. Neurosci., Article ASAP DOI: 10.1021/cn9000236. Publication Date (Web): November 25, 2009. Copyright © 2009 American Chemical Society.

After that cells were washed in PBS (3 × 15 min), counterstained with DAPI and mounted under coverslips with antifade mounting media iBright Plus (cat. no. SF40000-10; Neuromics, Inc.). Images of labeled cells were collected using Olympus FluoView1000 confocal microscope.
Images: High-magnification confocal images of double-labeling immunofluorescence for HA-δ and FLAG-μ opioid receptors. HEK-293 cells stably expressing both HA-δ and FLAG-μ opioid receptors are shown labeled for μ (A, green fluorescence) and for δ (B, red fluorescence). DAPI (blue fluorescence) has been used to stain the nuclei.

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