Thursday, August 31, 2006

Monoclonal Ubiquitin Antibodies-Great IHC

Ubiquitin is a protein that plays a role in the targeting of proteins for proteolytic degradation. Ubiquitin becomes covalently bonded to many types of pathological inclusions seen in neurodegenerative diseases that appear to be resistant to normal degradation. The neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, the Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can all be readily visualized with this antibody.


Image: ABC-HRP staining with Ubiquitin monoclonal antibody (diluted 1:1,000) of Alzheimer’s disease cerebral cortex showing dystrophic neuritis, which contain ubiquitinated tau. The inclusions are detected by the Ubiquitin antibody. Normal cortex does not contain inclusions and would be white in color.

Ubiquitin-Catalog#: MO18001

Confocal images of hippocampal CA1 sections from rats, (A) mock-treated or (B) subjected to 15 minutes ischemia followed by 24 hours of reperfusion, using ubiquitin mouse antibody (green) and propidium iodide (red). Data courtesy of Cell Signaling Technology, Inc.


Anonymous said...

I saw you mentioned ubiquitin so I thought you might be interested in Science Magazine's current webinar:
The Ubiquitin-Proteasome Pathway

Imgenex said...

Hello Dude,

Ubiquitin is a highly conserved 76 amino acid protein which is covalently coupled to proteins targeted for degradation by the proteasome, a normal physiologic process. In some cases, including those associated with pathological conditions, ubiquitin accumulation has been observed. This phenomenon has been seen in Alzheimer’s and Parkinson’s disease progression. Thanks a lot!

Ubiquitin Antibody