Neuron-Glial Antibodies Hit the Mark!

Neuromics' Neuron-Glial Markers offerings include:

Glial-Astrocyte Markers	Neural Progenitor Markers
Neuron/Synapse Markers	Oligodendrocyte, Oligodendroglial Oligodendrocyte Lineage Markers
Schwann Cell or PNS Neuronal Markers -Peripheral Nervous System (PNS) Related

They are widely used and frequently published. Customers have also shared data which is included in product descriptions. Here is a listing of the most recent pubs:
Wiebke Kallenborn-Gerhardt, Katrin Schröder, Domenico Del Turco, Ruirui Lu, Katharina Kynast, Judith Kosowski, Ellen Niederberger, Ajay M. Shah, Ralf P. Brandes, Gerd Geisslinger, and Achim Schmidtko. NADPH Oxidase-4 Maintains Neuropathic Pain after Peripheral Nerve Injury. The Journal of Neuroscience, 25 July 2012, 32(30): 10136-10145; doi: 10.1523/​JNEUROSCI.6227-11.2012.

Images: MPZ and PMP22 expression in the sciatic nerve of WT and Nox4−/− mice after SNI. A, Western blot analysis of the myelin-specific proteins MPZ and PMP22 in the day 14 SNI sciatic nerve (proximal nerve stump) and the uninjured control sciatic nerve. GAPDH was used as loading control. Note that MPZ and PMP22 protein expression is significantly decreased after SNI in WT mice but not in Nox4−/− mice. n = 3 mice per group. Data are presented as mean ± SEM (*p < 0.05). B, Immunostaining of the day 14 SNI sciatic nerve shows increased MPZ immunoreactivity in Nox4−/− mice compared with WT mice, whereas immunoreactivity of the neuronal marker NF200 is similar in both genotypes. Scale bar, 10 μm.

Maria Maddalena Valente, Valeria Bortolotto, Bruna Cuccurazzu, Federica Ubezio, Vasco Meneghini, Maria Teresa Francese, Pier Luigi Canonico, Mariagrazia Grilli.Alpha2delta ligands act as positive modulators of adult hippocampal neurogenesis andprevent depressive-like behavior induced by chronic restraint stress. Molecular Pharmacology Fast Forward Published on May 9, 2012 as doi:10.1124/mol.112.077636...chicken anti-nestin polyclonal (1:4,000, Neuromics, Edina, Minnesota)...

Frances Y. Cheng, Xi Huang, Anuraag Sarangi, Tatiana Ketova, Michael K. Cooper, Ying Litingtung, Chin Chiang. Widespread Contribution of Gdf7 Lineage to Cerebellar Cell Types and Implications for Hedgehog-Driven Medulloblastoma Formation. PLoS ONE 7(4): e35541. doi:10.1371/journal.pone.0035541...rabbit anti-GFAP (Neuromics, 1:500), mouse anti-GFAP (Neuromics, 1:200)...

Juan M Jimenez-Andrade and Patrick W Mantyh. Sensory and sympathetic nerve fibers undergo sprouting and neuroma formation in the painful arthritic joint of geriatric mice. Arthritis Research & Therapy 2012, 14:R101...to label primary afferent sensory nerve fibers, an antibody against neurofilament 200 kDa (NF200, chicken anti neurofilament 200 kDa; NF200, 1:5000; Neuromics; catalog #CH22104)...

I will be posting new developments.

Slowing Hearing Loss in Diabetics and Hyperglycemics

Our Neuronal-Glial Markers are used in a variety of applications. Here our P0 or P-Zero antibody is used to show myelination levels in the cochlear ganglion. Demyelination is caused by hyperglycemia and type 2 diabetes and results in hearing loss and eventually deafness. Here's the related publication and highlights: Silvia Murillo-Cuesta, Guadalupe Camarero, Águeda González-Rodríguez, Lourdes Rodríguez-de la Rosa, Deborah J Burks, Carlos Avendaño,Ángela M Valverde and Isabel Varela-Nieto1. Insulin Receptor Substrate 2 (IRS2)-Deficient Mice Show Sensorineural Hearing Loss That Is Delayed by Concomitant Protein Tyrosine Phosphatase 1B (PTP1B) Loss of Function. Online address: http://www.molmed.org. doi: 10.2119/molmed.2011.00328
Highlights: The authors objective was to study the hearing function and cochlear morphology of Irs2-null mice and the impact of PTP1B deficiency. They have studied the auditory brainstem responses and the cochlear morphology of systemic Irs2–/–Ptpn1+/+, Irs2+/+Ptpn1–/– and Irs2–/–Ptpn1–/– mice at different postnatal ages. The results indicated that Irs2–/–Ptpn1+/+ mice present a profound congenital sensorineural deafness before the onset of diabetes and altered cochlear morphology with hypoinnervation of the cochlear ganglion and aberrant stria vascularis, compared with wild-type mice. Simultaneous PTP1B deficiency in Irs2–/–Ptpn1–/– mice delays the onset of deafness.

Images:  Cochlear ganglion and nerve fibers. (A–C) Cresyl violet staining of midmodiolar methacrylate sections of the cochlear ganglion at the cochlear basal turn in Irs2+/+Ptpn1+/+ (A), Irs2–/–Ptpn1+/+ (B) and Irs2–/–Ptpn1–/–mice (C) at postnatal wk 11. A slight reduction in the cellular density was more evident in both mutants (B, C) compared with wild-type (A). (D–F) Myelin P0 immunostaining of the cochlear ganglion shows less intense labeling in Irs2–/–Ptpn1+/+ (E) and Irs2–/–Ptpn1–/– (F) mice than in control mice (D). (G–I) Accordingly, myelin P0 immunostaining of nerve fibers projecting from the cochlear ganglion to the sensory cells is less intense in Irs2–/–Ptpn1+/+ and Irs2–/–Ptpn1–/– (arrows in H and I) than in wild-type mice (G). (J–L) Similarly, a weaker neurofilament 200-kDa immunostaining is observed in Irs2+/+Ptpn1–/– (K, white arrow) and Irs2–/–Ptpn1–/– (L, white arrow) mice compared with wild-type mice (J). Scale bars: A–I, 50 μm; J–L, 75 μm.

The results presented in this study demonstrate for the first time a unique tissue-specific role of IRS2 in cochlear development and hearing function; therefore, the Irs2–/– mouse could be a novel model for the in vivo study of hearing loss associated with altered glucose metabolism. The data also suggest that modulation of PTP1B activity could be a pharmacological target of interest for the sensory syndromes associated with diabetes.