Neuro-Toxicity Co-Culture

Poster Presentation at Society of Toxicologist Annual Meeting 2015.

As part of Neuromics' strategic selling partnership with ArunA Biomedical. We are attending the SOT 2015 meeting in San Diego, CA.

I am particular excited about our getting the word out on our solutions via poster presentations. Here's the line up:

“In-vitro Human Developmental Neurotoxicity Screening Using Multiple Cell Types"

Anirban Majumder¹, Xian Wu^2,3, Shelley Wallace¹, Jane Le¹, Steven L. Stice^1,2,3

 ¹ArunA Biomedical, Inc. Athens, GA, USA, ²Regenerative Bioscience Center, ³Interdisciplinary Toxicology Program, University of Georgia, Athens, GA, USA

Abstract Number/Poster Board number: 636 Poster Board -443

Presentation: March 23, 2015 1:00 PM to 4:30 PM, CC Exhibit Hall 

“Mouse Pluripotent Stem Cell Motor Neurons Generate Robust Neural Network Activity on Microelectrode Arrays"

Steven L. Stice^1,2 Anirban Majumder¹, Brad Culp¹, Anthony M. Nicolini³ and Colin Arrowood³

 Aruna Biomedical Inc. ¹, Regenerative Bioscience Center, Univ. of Georgia, Athens GA. ², Axion BioSystems, Atlanta, GA³

Abstract Number/Poster Board number: 279 Poster Board - 450

Presentation: March 23, 2015 from 9:00 to 12:00 CC Exhibit Hall

 “Bisphenol-A effects on in vitro Human Neural Development was Window of Susceptibility dependent"

Xian Wu^1,2, Anirban Majumder³, Steven L. Stice^1,2,3

¹Interdisciplinary Toxicology Program, ²Regenerative Bioscience Center, University of Georgia, Athens, GA, USA  ³ArunA Biomedical, Inc. Athens, GA, USA  

Abstract Number/Poster Board number: 249 Poster Board - 415

Presentation: March 23, 2015 from 9:00 to 12:00 CC Exhibit Hall

“MR Imaging of Human Neural Progenitor Stem Cells: An In Vivo Longitudinal Model "

Forrest Goodfellow, Qingying Ming, Xian Wu, Erin Jordan, Qun Zhao, Steve Stice

Interdisciplinary Toxicology Program, University of Georgia, Athens, Georgia 30602

Abstract Number/Poster Board number: 641 Poster Board -448

Presentation: March 23, 2015 from 1:00 PM to 4:30 PM CC Exhibit Hall 

"Using Human-Derived Neural Cells As an In Vitro Model for Developmental Neurotoxicity following Exposure to Pesticides"

Mary Smith^1,2 , Mayowa Amosu¹ , Xiaoming Bian¹ , Kun Lu¹ , Steven Stice^2,4 , William Henderson³ , Shelley Wallace⁴ Anirban Majumder⁴

¹Department of Environmental Health Science, University of Georgia, Athens, Georgia, United States; ² Regenerative Bioscience Center, University of Georgia, Athens, Georgia, United States; ³ ORD/NERL/ERD, U.S. EPA, Athens, Georgia, United States; ⁴ ArunA Biomedical, Inc., Athens, Georgia, United States

Abstract Number/Poster Board number: 1747 Poster Board - 152

Presentation: March 23, 2015 from 9:00AM  to 12:00 PM 

More to follow...

Neuromics-ArunA Biomedical Partnership

We are Closer than Ever!

I spent the past several days meeting with Ms. Tracey Stice (COO), Dr. Steven Stice (President and CSO) and Ms. Joy Clark (Sales and Marketing Manager) at ArunA HQ in Athens, GA. The objective of the meeting was to finalize plans for our Strategic Selling Partnership. This partnership enables us to open up more cell based assay solutions to current and future Customers involved in neuro-diseases/disorders research and drug discovery.

The Aruna Team

...And the news gets better. By combining our expertise and experience, we are now well positioned to to:

1. Shape new solutions and services that would more tightly aligned with your unique requirements
2. Communicate how other labs within your organization are using our solutions and look for ways to leverage this.
3. Track buying volume so we can give favorable pricing based on purchase made in total by your organization vs specific groups.

We will be working even harder to make sure you that you are satisfied with the value we bring to you and your team. Wondering what we can do for you today? You can contact me directly 612-801-1007 or pshuster@neuromics.com. Thank you. Pete Shuster, CEO, Neuromics AND ArunA Biomedical Strategic Selling Partner.

STEMEZ hNP1 Neural Progenitors Now Available

We are pleased to announce the introduction of our STEMEZ ^TMhNP1 Human Neural Progenitors Discovery Kit. The kit was developed by our partner, ArunA Biomedical.

Description/Data:
STEMEZ^TM hNP1 Human Progenitors are fully differentiated are derived as adherent cells from hESC WA09 line. The cells are shipped frozen in a vial with 1 x 10⁶ cells; once thawed they should be immediately plated on a Matrigel (or other suitable extracellular matrix protein)-coated dish and maintained in the accompanying serum-free medium. The neurons should be used within 14 days of thawing. These cells have the capabilities to:

• Display immunoreactive properties consistent with neural precursors and can be maintained in a proliferative state in monolayer cultures.
• Differentiate under serum-free conditions into neuronal phenotypes with functionally responsive transmitter receptors in vitro .
• Maintain multiple neural phenotypes in long term serum-free culture.
• Can be directed to alter phenotypic characteristics under different media conditions.

Applications: Gene Expression Analysis Western blotting, Flow Cytometry, Immunocytochemistry, FACS sorting, DNA Microarray, RT-PCR, Neurite Outgrowth assays, FLIPR calcium assays, cytotoxicity, and second messenger signaling.

Images: STEMEZ^TM hNP1 Human Neural Progenitor Cells are grown as monolayers (A), are karyotypically normal (B) and express NSC markers, Nestin Mouse and Sox-2 (C, D, E). Nuclei of the cells were visualized with DAPI (blue). The Sox-2 transcription factor is co-localized with the DAPI (blue) staining in the nucleus (F).

STEMEZ hNP1 FAQs

Note: STEMEZTM NP1 Progenitors and hN2 Human Neurons can be genetically manipulated with GFP reporters without altering the function and differentiation potential of these cells. In contrast to many stem cell sources, the reporter genes are not readily silence in the neural cultures. See: doi:10.1089/ten.tea.2009.0155.

DNA fingerprint cells: The loci match the DNA fingerprint pattern for the H9 (NIH designation, WA09) HESC line as published in http://stemcells.nih.gov/research/nihresearch/scunit/.

Viral tests cells: This lot was derived from the H9 hESC line that has been tested for Hepatitis B, Hepatitis C, HIV-1, HIV-2, HTLV-I/II, HSV1, HSV2, EBV, and CMV. The H9 cell line has been tested and shown to be negative. (Tests performed by GIVF Laboratories).

Related Reagents:

• STEMEZ(TM) hN2 Human Neurons Discovery Kit


• Neuron/Glial Marker Antibodies


• Neurotrophins and Growth Factor Antibodies


• Fibroblast Growth Factor Proteins


• Neurotrophins-Neuron/Glial Marker Proteins


• Stem Cell Reagents


• Transfection Reagents

STEMEZ hN2 Human Neurons-Data

Neuromics rolled out STEMEZ^TM hN2 Human Neurons Discovery Kits several months ago.

Applications for these include: cellular model studies, high content screening, developmental studies, RNAi studies and genetic manipulation.

Drilling down further, I am pleased to present Electro-physiology and related data generated by Aruna and collaborators: hN2 Cells-Electro Phys Data Supplement

hN2-Whole Cell Voltage Clamp
Figure. hN2 cells can produce inward currents that generate action potentials. (A) Isolated hN2 with significant neurite growth 1 week after plating . This cell was subjected to whole cell voltage clamp utilizing a potassium gluconate based intracellular solution. (B) Voltage gated inward and outward currents were elicited from this cell with depolarizing voltage steps. (C) Inward currents from another cell (potassium gluconate intracellular) were abolished by local application of 1 µM tetrodotoxin (red trace) while outward currents remained. Inward current recovered as TTX washed out of the region (green trace). (D) A different cell which exhibited voltage activated inward currents that inactivated in response to a 50 ms prepulse at different membrane potentials. The experiment was done 27 days after the removal of bFGF. A cesium gluconate based intracellular solution was used for this experiment to block outward potassium currents. The membrane potential for half maximal inactivation by standard Boltzman fitting (red line) was -40.1 mV with a slope of 4.7. (E) Recovery from fast inactivation utilizing a paired pulse protocol in the same cell as C. The single exponential time constant for recovery of inactivation was 1.7 ms (red line). (F) A different cell which elicited an overshooting action potential upon current injection under whole cell current clamp utilizing a potassium gluconate based intracellular solution. Inset: Response of the same cell under voltage clamp to a change in membrane potential from -80 mV to -10 mV elicited a peak current of 457 pA. Scale bars for inset: 5 ms, 0.2 nA.

Introducing Human Neuron Kits

hN2 Human Neurons Discovery Kit-New

Energize you Research!
Neuromics has formed an alliance with Aruna Biomedical. This Alliance gives us the capabilities to bring you the reliable, robust and highly scalable hN2TMHuman Neurons Discovery Kits.
These kits are designed to reduce basic Neuroscience Research and Drug Discovery timelines. Potential applications include: cellular model studies, high content screening, developmental studies, RNAi studies and genetic manipulation.
Approximate Yield=1,000,000 healthy Neurons.

hN2 Human Neurons Discovery Kit Details