Wednesday, June 20, 2012

TRPA1 and Tooth Pain

Our TRPV (Vanilloid); TRPM; TRPA and TRPCs have proven excellent for studying

Transient receptor potential ankyrin 1 (TRPA1) is activated by noxious cold (<17°C) and contributes to cold and mechanical hypersensitivity after inflammation and nerve injury: Yun Sook Kim, PhD, Hoon Kap Jung, DDS, Tae Kyung Kwon, DDS, Chin Soo Kim, DDS, PhD, Jin Hyun Cho, DDS, PhD, Dong Kuk Ahn, DDS, PhD, Yong Chul Bae, DDS, PhD.Expression of Transient Receptor Potential Ankyrin in Human Dental Pulp. Journal of Endodontics. Available online 8 June 2012.

Highlights: TRPA1 was expressed in a large number of axons branching extensively in the peripheral pulp and in a few axons within the nerve bundles in the core of the coronal pulp and in the radicular pulp. Under electron microscopy, TRPA1 immunoreactivity was typically localized near the plasma membrane of unmyelinated axons in the peripheral pulp, suggesting that in these axons it may act as a functional receptor. The proportion of axons expressing TRPA1 in neurofilament 200–positive axons significantly increased in the painful pulp compared with the normal pulp. TRPA1 was also densely expressed in the processes and the cell body of odontoblasts. A large number of axons coexpressed TRPA1 and Nav1.8.

Images: Immunofluorescent staining for (A) TRPA1 in the human normal dental pulp is completely abolished by (B) preadsorption with a control peptide, proving the specificity of the TRPA1 antiserum (×200, scale bars = 50 µm).
Related Reagents:
All TRP Antibodies                       
Pain and Inflammation Research Antibodies                     
Neurotransmission -Neurotransmission Research Antibody Categories
Primary Neurons and Astrocytes-Primary human, rat and mouse neurons and astrocytes

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