Thursday, April 26, 2012

GFAP Antibodies-Daily Double

I consider publications important for confirming our Neuronal-Glial Markers are working as advertised. These tools often used by our customers for lineages of differentiating Neural Progenitors. This publication references use of our Mouse Monoclonal GFAP and Rabbit Polyclonal GFAP Antibodies: Frances Y. Cheng, Xi Huang, Anuraag Sarangi, Tatiana Ketova, Michael K. Cooper, Ying Litingtung, Chin Chiang. Widespread Contribution of Gdf7 Lineage to Cerebellar Cell Types and Implications for Hedgehog-Driven Medulloblastoma Formation. PLoS ONE 7(4): e35541. doi:10.1371/journal.pone.0035541.
Abstract:The roof plate is a specialized embryonic midline tissue of the central nervous system that functions as a signaling center regulating dorsal neural patterning. In the developing hindbrain, roof plate cells express Gdf7 and previous genetic fate mapping studies showed that these cells contribute mostly to non-neural choroid plexus epithelium. We demonstrate here that constitutive activation of the Sonic hedgehog signaling pathway in the Gdf7 lineage invariably leads to medulloblastoma. Lineage tracing analysis reveals that Gdf7-lineage cells not only are a source of choroid plexus epithelial cells, but are also present in the cerebellar rhombic lip and contribute to a subset of cerebellar granule neuron precursors, the presumed cell-of-origin for Sonic hedgehog-driven medulloblastoma. We further show that Gdf7-lineage cells also contribute to multiple neuronal and glial cell types in the cerebellum, including glutamatergic granule neurons, unipolar brush cells, Purkinje neurons, GABAergic interneurons, Bergmann glial cells, and white matter astrocytes. These findings establish hindbrain roof plate as a novel source of diverse neural cell types in the cerebellum that is also susceptible to oncogenic transformation by deregulated Sonic hedgehog signaling.

Images: A subset of Gdf7-lineage cells express neural stem cell markers.(A–B″′) Many cells within the tumor tissue of Gdf7Cre/+;SmoM2 mice coexpress neural stem cell marker Nestin (red) and glial marker GFAP (green). Arrows indicate co-localization.

I will continue to post publication and customer data showing results with our Neuron-Glial Markers.

1 comment:

Drug Discovery said...


Nice post. GFAP is an intermediate filament protein and acts as an intracellular structural component of the cytoskeleton, which is also expressed by radial glial cells of the CNS. Various mutations of the GFAP gene in humans produce. I like the way you start and then conclude your thoughts. Thanks for this information....