Stem Cell Markers like PAX7 are important for types of studies. PAX7 is a marker for muscle progenitors or stem cells. If these cells fail to differentiate the level of PAX7 expression will remain high. MyoD is an indicator for successfully differentiating cells. As a consequence, MyoD expression levels increase within the cell and the ratio of Pax7:MyoD is reduced, thus inhibiting proliferation and inducing differentiation through Myogenin expression. In the absence of TRIM32, this balance of Pax7:MyoD is dysregulated, because degradation of c-Myc is not initiated, MyoD levels do not increase and Myogenin expression is not activated.
Figure . Differentiation is strongly reduced in satellite cell progeny on TRIM32−/− myofibers. (a), (c) and (e) Immunostainings of satellite cells on freshly isolated myofibers from wild type (+/+) and TRIM32−/− (−/−) mice cultured for 24 h (a), 48 h (c) and 72 h (e) and labelled with the indicated markers (upper grey boxes). (b), (d) and (f) Diagrams showing the relative frequency of Pax7+/MyoD− cells on fibers cultured for 24 h (b), Pax7+/Myogenin− cells on fibers cultured for 48 h (d) and Pax7−/Myogenin+ cells on fibers cultured for 72 h (f). In all cases cells on myofibers from wild type mice and TRIM32−/− mice are compared. (mean ± std; *P<0.001 compared to wild type).
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