siRNA-mediated gene silencing

Dr. Josephine Lai (Professor of Pharmacology, University of Arizona) is a pioneer in developing experimental designs and methods for delivering siRNA to the CNS for gene expression analysis.

She and her team have documented these in the publication:

• Modulating Sensory Systems Using RNAi(pdf - 187Kb)
  © 2007 Lai

For researchers desiring to effectively deliver siRNA to the CNS for gene expression of analysis of specific receptors, this publication offers proven methods. These include:

• The Choice of siRNA
• Choosing and Optimizing Transfection Reagents for siRNA Delivery to the Nervous System
• Delivery Systems-Microinjection and Infusion (using mini-osmotic pumps)
• Validation

We will continue to track advances by Dr. Lai and team