Geneviève Roussy, Marc-André Dansereau, Louis Doré-Savard, Karine Belleville, Nicolas Beaudet, Elliott Richelson and Philippe Sarret. Spinal NTS1 receptors regulate nociceptive signaling in a rat formalin tonic pain model. doi:10.1111/j.1471-4159.2007.05205.x
Images: Expression of NTS1 receptors in sensory neurons and spinal cord. (a)
Immunoperoxidase staining reveals the presence of NTS1-like immunoreactivity throughout the lumbar dorsal horn of the spinal cord. Immunolabeling is most prominent in the superficial layers of the dorsal horn. The lateral spinal nucleus (lsn) is also moderately labeled (arrow). (b) High magnification of (a). A dense NTS1 immunostaining is observed over laminae I and II of the dorsal horn. Numerous immunopositive nerve cell bodies are visible in the superficial laminae and in the nucleus proprius of the dorsal horn (arrows). (c) Light microscopic analysis of NTS1 expression in primary afferent neurons. NTS1 is expressed in subpopulations of small- and medium-ganglion cells. No apparent labeling is detected in large DRG neurons (arrows). At higher magnification, NTS1 neurons exhibit a cytoplasmic pattern of immunoreactivity (d, arrowheads). (e) Identification of endogenously expressed NTS1 receptors, by western blotting, in homogenates from DRGs, lumbar spinal cord and brain. The 47 kDa protein band corresponds to the molecular weight deduced from the cDNA sequence of NTS1. Each lane represents the transfer of 25 lg of protein. Scale bars: (a, b, c, and d) 300, 150, 70 and 20 lm, respectively. doi:10.1111/j.1471-4159.2007.05205.xPrimary Neurons and Astrocytes
Cortex
Hippocampus
Striatum
E18 and E20 Rat Primary Neuronal Tissue -NEURON CULTURES
E18 Rat Primary Neuronal Tissue - ASTROCYTE CULTURES
E18 Mouse Neuronal Tissue -NEURON CULTURES
E18 Mouse Neuronal Tissue -ASTROCYTE CULTURES
Frozen Primary Rat Neurons-NEW
Frozen Primary Mouse Neurons and Astrocytes-New
FluoGreen Tracer-Stains Cell Nuclei and Cytoplasm
Primary Neurons and Astrocytes
Shouqing Luo, Haruo Mizuta and David C. Rubinsztein. p21-activated kinase 1 promotes soluble mutant huntingtin self-interaction and enhances toxicity. Human Molecular Genetics 2008 17(6):895-905; doi:10.1093/hmg/ddm362
...Rat E18 cortical and striatal neurons were purchased from Neuromics (MN) or prepared from E18 rat brain tissues according to instructions from Neuromics, cultured in neurobasal (Invitrogen) medium supplemented with B27 (Invitrogen) and 0.5 mM glutamine and transfected with Lipofectamine 2000 according to standard methods...
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