Sunday, June 25, 2006

New Publication-Neuromic's E-18 Primary Hippocampal Neurons

Brian Hoffpauir , Emily McMains, and Evanna Gleason Nitric Oxide Transiently Converts Synaptic Inhibition to Excitation in Retinal Amacrine Cells J Neurophysiol 95: 2866-2877, 2006

Hippocampal cultures were made from 18-day-old rat (Fischer 3440) embryos. Micro-dissected hippocampal tissue was obtained from Neuromics (Bloomington, MN) and prepared and maintained according to the supplier’s protocols. Recordings from single hippocampal neurons were made after 4–7 days in culture. No physiological assessments of the relative maturity of these cells have been made. According to their developmental timeline alone, they would be considered embryonic.

Thursday, June 15, 2006

New Goat VR1 (TRPV1) and Rabbit NK1 Antibody

Catalog #: GT15129

Image: VR1 staining in Rat DRG: red color - TRPV1 labeling, green-counterstain.

Description: VR1, also known as TRPV1 (Transient Receptor Potential Vanilloid 1) and capsaicin receptor, is a 115 kDa integral membrane ion channel that has six transmembrane domains and contains intracellular N-and C-termini. It contributes to normal pain and temperature sensation and also has a "sensory-effector" function. By alternative splicing, at least three VR1 isoforms are known. The sequence of residues 4 - 21 of rat VR1 is 78% and 28% identical to that of mouse and human VR1, respectively.

Catalog #: RA25001

Image: NK 1 staining of lamina III neuron in the spinal cord of a rat.

Description: The tachykinins belong to an evolutionary conserved family of peptide neurotransmitters that share the c-terminal sequence Phe-X-Gly-Leu-Met-NH2 and have an established role in neurotransmission. The mammalian tachykinins include substance P, neurokinin A (NKA) and neurokinin B (NKB) which exert their effects by binding to specific receptors. Tachykinin peptides are important in the mediation of many physiological and pathological processes including inflammation, pain, migraine headache and allergy induced asthma. Three tachykinin receptor types have been characterized, NK-1, NK-2 and NK-3 which have preferential affinities for SP, NKA and NKB respectively. All three receptors share a high degree of sequence homology, have seven transmembrane spanning domains and similar signal transduction mechanisms (e.g. G-protein coupled activation of phospholipase C).

Wednesday, June 07, 2006