Monday, October 16, 2006

P2X3 Western Blot

There have recently been customer question on the expected band for our P2X3 Antibody (Catalog#: RA10109)
Here's an excellent publication showing both WB and IHC for this antibody.

P2X2 and P2X3 receptor expression in postnatal and adult rat urinary bladder and lumbosacral spinal cord Simon Studeny, Ali Torabi and Margaret A. Vizzard.

Figure: Western blot of whole urinary bladder (40 mg) for P2X3 receptor expression in postnatal (P0-P36) and adult (A) rats. Erk staining was used as a loading control. B. Preabsorption of P2X3 antibody with immunogen (1 mg ml−1) eliminated the band at 75kD. C. Histogram of relative P2X3 band density in all groups examined normalized to erk-1 staining. P2X3 receptor expression in urinary bladder is significantly increased from P14-P21 compared to adult rat bladder. *, p ≤ 0.005, #, p ≤ 0.01.

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