Sunday, January 29, 2023
Thursday, November 17, 2022
Widely Used and Data Rich
Neuromics Human Brain Microvascular Endothelial Cells (HBMECS) are frequently used and data rich. Here are HBMECS in culture showing early microtubular formation.
Thursday, October 13, 2022
Breast CAFs in culture. Grown using Stem Cell Complete Low Serum Media (cat. CAFM02)
Cells are prepared and manufactured in an ISO 9001:2015 certified environment and ISO 5 cleanroom.
Questions? email email@example.com
Friday, July 29, 2022
Our primary human neurons (cat. HNC001) have been busy lately, continuing to become one of our most popular and versatile products. Earlier this month, Japanese researchers published their findings when creating 3D brain-like spheroids containing our primary human neurons. Their results suggest the spheroids mimic in vivo conditions and are suitable for use as an in vitro neurological disease model. Check out the publication for yourself here.
Human Neurons in Culture
Since the beginning of 2022, our neurons have been cited a number of times spanning various research areas. They were used by researchers to suggest a link between the COVID-19 virus and Alzheimer's disease (lean more here). Additionally, investigators published findings (see here) using the neurons to find a potential therapeutics target for neuroblastoma.
Questions? Do not hesitate to contact me firstname.lastname@example.org or 612-801-1007. Pete Shuster-CEO and Owner
Wednesday, April 20, 2022
Tuesday, February 15, 2022
Check out the OptionsNeuromics is pleased to introduce many of the top human prostate cancer cell lines to our already expansive selection of cell offerings. To complement the DU 145 cells we added earlier this year, Neuromics is now offering VcaP, LNCaP, and PC-3 cells to complete our catalog of human prostate cancer cell lines.
Tuesday, January 25, 2022
Friday, January 21, 2022
Hungarian researchers used our guinea pig polyclonal TRPV1 receptor antibody (GP14100) to help answer why heightened insulin levels can lead to migraines.
Immunohistochemistry in rat trigeminal ganglion. (A) Trigeminal ganglion neurons in the ophthalmic division immunoreactive for insulin receptor (InsR), TRPV1 receptor and CGRP. (B) Coexpression of insulin receptor (InsR) with TRPV1 receptor and/or CGRP in trigeminal ganglion neurons. Colours representing insulin receptor-, TRPV1 receptor- and CGRP-immunoreactivities and their coexpressions indicated in B applies also to A. (C) A trigeminal ganglion neuron retrogradely labeled with True blue expresses both insulin receptor (InsR) and TRPV1 receptorConclusion-the present findings indicate that insulin may activate TRPV1 receptors in the trigeminovascular system. Modified TRPV1 receptor function induced by insulin may also increase the sensitivity of both neural and vascular TRPV1 receptor for its agonists. Our data may provide a pathophysiological basis for the increased incidence of migraine in patients with hyperinsulin levels.
Friday, December 31, 2021
Wednesday, November 24, 2021
Check out How Neuromic's Tools Are Used
The authors used four of Neuromics to complete this study-Sulodexide reduces glucose induced senescence in human retinal endothelial cells A. Gericke, K. Suminska-Jasińska & A. Bręborowicz Scientific Reports volume 11, Article number: 11532 (2021) Cite this article.
Saturday, October 23, 2021
Best Price and Performance
I was honored to work with Jessey at National Cancer Institute (NCI).
The were determining whether to change partners. According to Jessey, there were 24 lab PIs who would be supplied with the winner's FBS Although we proved competitive, we were not selected.
Wednesday, October 06, 2021
Compound Penetration Study
Thursday, June 03, 2021
Human Renal Proximal Tubule Cells Express Klotho
Our human primary cells are widely used and frequently referenced in a variety of publications. In this study, our RPT Cells were used to study the impact of Klothko expression on protection of kidneys in diabetics: Meng Xue, Feng Yang, Ying Le, Yanlin Yang, Bingsen Wang, Yijie Jia, Zongji Zheng, and Yaoming Xue. (2021). Klotho Protects Against Diabetic Kidney Disease via AMPK- and ERK-Mediated Autophagy. Acta Diabetologica, 32. doi: 10.1007/s00592-021-01736-4.
Thursday, May 27, 2021
Markers for Pain Research
Trigeminal Neuralgia is a progress chronic condition. Dysfunctional trigeminal signaling can lead to intense, searing facial pain. It is caused by pressure on the trigeminal nerve.
The root causes of the condition are not well understood. Our pain research markers have been widely used and frequently published through the years and have been important contributors to our growth, Here's a recent pub referencing use of one our P2X3 Antibodies-Momoko Koizumi, Sayaka Asano, Akihiko Furukawa, Yoshinori Hayashi, Suzuro Hitomi, Ikuko Shibuta, Katsuhiko Hayashi, Fusao Kato, Koichi Iwata, and Masamichi Shinoda. (2021). P2X3 Receptor Upregulation in Trigeminal Ganglion Neurons Through TNFα Production in Macrophages Contributes to Trigeminal Neuropathic Pain in Rats. The Journal of Headache and Pain, 22, 31. doi: 10.1186/s10194-021-01244-4
Images: Changes in P2X3R expression in TG neurons innervating whisker pad skin and the involvement of P2X3R signaling in TG neurons in orofacial mechanical hypersensitivity on day 7 following TNC. a Photomicrograph of FG-labeled P2X3IR TG neurons following the TNC or sham procedure. Arrows indicate FG-labeled P2X3IR TG neurons. Scale bar: 100 μm. b Mean number of FG-labeled TG neurons ipsilateral to the TNC or sham procedure (n = 5 in each). c The frequency of FG-labeled P2X3-IR TG neurons ipsilateral to the TNC or sham procedure. * p < 0.05 vs. sham. (n = 5 in each, student’s t-test). d The time course of changes in the MHWT by subcutaneous A317491 or TNP-ATP administration on day 7 following the TNC or sham procedure.
The signaling of TNFα released from the activated macrophages and infiltrated into and/or proliferated in the TG induces the upregulation of P2X3R expression in TG neurons innervating the orofacial region, resulting in orofacial mechanical allodynia following TNC. Consequently, TG neuronal P2X3R hyperexpression by enhanced TNFα signaling in the TG is a potential target for TN treatment..
Monday, February 01, 2021
EPCR and ICAM Receptors
Neuromics Astrocytes and Pericytes were used in this important study-Yvonne Adams, Rebecca W. Olsen, Anja Bengtsson, Nanna Dalgaard, Mykola Zdioruk, Sanghamitra Satpathi, Prativa K. Behera, Praveen K. Sahu, Sean E. Lawler, Klaus Qvortrup, Samuel C. Wassmer, and Anja T.R. Jensen. (2021). Plasmodium Falciparum Erythrocyte Membrane Protein 1 Variants Induce Cell Swelling and Disrupt the Blood–Brain Barrier in Cerebral Malaria. Journal of Experimental Medicine, 218 (3). doi: 10.1084/jem.20201266.
Tuesday, January 26, 2021
Sometimes we hear our human cells did not express a certain receptor in the hands of our customers. We guarantee our cell "walk and talk" as advertised. If you are ever in doubt. we will test for expression of any receptors or cytoplasmic proteins. We also do this as part of our quality checks.
Here're, for example, is a pub showing our Schwann Cells working. Note the receptors tested here. Seung Min Shin, Francie Moehring, Brandon Itson-Zoske, Fan Fan, Cheryl L. Stucky, Quinn H. Hogan, and Hongwei Yu. (2021). Piezo2 Mechanosensitive Ion Channel is Located to Sensory Neurons and Non-Neuronal Cells in Rat Peripheral Sensory Pathway: Implications in Pain. bioRxiv. doi: 10.1101/2021.01.20.427483
Wednesday, November 25, 2020
Neuromics' Colorectal CAFS in Action
In a first of its kind study, the mechanical properties of our CAFS were determined. This is important because, understanding how these cell behave in vivo will help improve the efficacy of new therapies: Bashar Emon, Zhengwei Li, Md Saddam Hossain Joy, Umnia Doha, Farhad Kosari, and M Taher A Saif. (2020). A Novel Method for Sensor-Based Quantification of Single/Multi-Cellular Traction Dynamics and Remodeling in 3D Matrices. bioRxiv. doi: 10.1101/2020.09.24.311647
"Cells in vivo generate mechanical forces (traction) on surrounding 3D extra cellular matrix (ECM) and cells. Such traction and biochemical cues may remodel the matrix, e.g. increase stiffness, which in turn influences cell functions and forces. This dynamic reciprocity mediates development and tumorigenesis. Currently, there is no method available to directly quantify single cell traction and matrix remodeling in 3D. Here, we introduce a method to fulfil this long-standing need. We developed a high-resolution microfabricated sensor which hosts a 3D cell-ECM tissue formed by self-assembly. It measures cell forces and tissue-stiffness and can apply mechanical stimulation to the tissue. We measured single and multicellular force dynamics of fibroblasts (3T3), human colon (FET) and lung (A549) cancer cells and cancer associated fibroblasts (CAF05) with 1 nN resolution. Single cells show significant force fluctuations in 3D. FET/CAF co-culture system, mimicking cancer tumor microenvironment, increased tissue stiffness by 3 times within 24 hours."
Here's one of 10 videos illustrating these properties.
CAFS are widely used and frequently published. Check them out today!
Friday, October 23, 2020
Gene Expression Tools in Action
Neuromics has a wide offering of transfection reagents that are perfect for both in vivo and in vitro applications. We take pride in seeing our product used in research, which is certainly true of our transfection reagents (see here).
Furthermore, we'd like to highlight a recent use of our n-Fect Transfection Kit (cat.# NF30150) in a doctoral thesis recently published. The paper looked into the neuroscience of alcohol addiction, looking towards the purinergic family receptor P2X4, which has been linked to alcohol addiction in mouse models. Using our n-Fect Transfection Kit, the molecular and cellular mechanisms linking P2X4 expression and voluntary alcohol consumption are explored. Our n-Fect reagents were used on brain slices containing the ventral tegmental area (VTA) of mice. They conclude that purinergic control of VTA neurons is a neurotransmitter system that should be further studied as a target for alcohol dependence drugs.
Image: Examples of p-Fect (Cat.# PF3000) transfection in different cell types.
Questions? Do not hesitate to contact Rose Ludescher, VP of customer satisfaction -email@example.com
Saturday, September 19, 2020
There is a difference
Here's a video that illustrates the difference between CDC recommended Virus Transport Media and Sterile PBS (salt water). It answers the questions "what is in your VTM" and "why does it matter". Please consider sharing so it can find its way to those in need!
Thursday, August 20, 2020
Blood-Brain Barrier (BBB) Permeability Assay
Yes, These are troubled times. With our current focus on eradicating COVID-19, we forget that many of the world's most insidious and costly diseases have limited treatments and no cures.
That is why Neuromics continues to develop its human based 3-D Cell-Based Assays. At the center if our offerings is our BBB Model.
Here's a recent patent application that includes a permeability assay using our model-Sookhee Bang, Jeong Kuen Song, Seung-wook Shin, Kwan Hee Lee, and Ho June Lee. (2020). Method of Treating Central Nervous System Disease. United States Patent Application 20200230218BBB Permeability Assay of AL04 The in vitro human BBB model (Neuromics, USA) was established using co-cultures of primary Human brain endothelial cells (HBEC), Human brain pericytes (HBPC), and Human brain astrocytes (HBAC). In vitro human BBB model kit has two sides (luminal, blood/abluminal, brain) with 12 transwell inserts (polyester membrane, 0.4 um pore, diameter 12 mm, insert growth area: 1.12 cm2). HBPC were grown on the bottom side of the inserts, HBEC were monolayered on the upper side of the inserts, and HBAC were grown on the bottom of the 12-well culture plate (Neuromics, USA). In vitro BBB model was activated according to the manufacturer's instructions for 4 days. Briefly, the medium from both luminal and abluminal (lower, brain) sides of the transwell insert was changed every other day. Before the transport experiment, the abluminal side was filled with the permeability assay medium. For the permeability assay, purified AL04 or recombinant HSA (Sigma, USA) were added to the luminal side (0.3 ml) of the transwell insert to yield a final concentration of 1 or 10 uM. Incubations were performed on orbital shaker (100 rpm) at 37° C. Samples (150 μl) were collected from the abluminal side (1.2 ml) at 60, 120 and 240 min and immediately replaced with fresh permeability assay medium. The concentrations of transported AL04 or rHSA were measured by Human albumin quantitation ELISA kit (Bethyl laboratory, USA) and analyzed using the standard curve method. Permeability coefficients (Pe, cm s−1) were calculated using the equation: Pe=(VA/(A×c0))×(dQ/dt), where VA is volume of assay buffer in blood side (inside of the insert), A is the surface area of the insert (1.12 cm2), c0 the initial concentration of protein sample added into blood side, dQ/dt the concentration of transported protein sample in brain side in a defined time period. The permeability coefficients (Pe, cm s−1) for the purified AL04 or recombinant HSA were calculated as previously described (Prades, 2015; Nakagawa, 2009).
Monday, July 27, 2020
Neuromics is known to have some of the best Fetal Bovine Serum (FBS) for most applications. This includes being a component of VTM manufactured to the CDC's most recommended recipe.
Thursday, June 25, 2020
We are well on our way to delivering 100,000 Tubes + VTM to the state of Ohio. A big thank you to my team and friends for making this happen.
Saturday, May 16, 2020
From Seattle, Washington to New York City and in between, our Virus Transport Medium has been used in COVID-19 testing. Neuromics is doing our part by helping to expand testing capacity, allowing the nation to recover from the harships associated with the pandemic.
Our VTM is manufactured using our FBS following CDC guidelines, ensuring that it is sterile and safe for testing. Additionally, each lost of VTM goes through quality control before release (sample CoA).
We offer our VTM in many different forms, including kits (tubes and sterile swabs) and larger quantities that can be aliquoted into tubes under a sterile hood.
If you have immediate needs please email david@neuromics,com, Manager, COVID-19 Testing Supplies and kits.
Wednesday, April 15, 2020
We have Virus Transport Media in tubes with 2 options.
VTM-1: 2 ml 15 ml tube (100 mm) - qty 15 (Available after 4/24)
VTM-2: 2 ml 15 ml tube (120 mm) - qty 15
We can also provide tubes with custom volumes of VTM.
Wednesday, April 08, 2020
Though rare, we occasionally have clients challenge the phenotype of our 21-CFR compliant human primary cells. This challenge is usually a result of certain markers not being expressed when they run immuno-fluorescence assays.
When this happens, we too test the cells in question using the antibodies against the markers in question. We have a world class team doing immuno-fluorescence assays.
Recently a customer claimed our schwann cells were negative for GFAP and P0. Here're our testing results.
Thursday, March 26, 2020
Tuesday, March 17, 2020
If you call us, we will answer the phone. If you submit an order, it will ship.
Neuromics stands ready to serve you.
Monday, February 24, 2020
Monday, February 10, 2020
We are always honored when you share your data with us! To learn more check out the video.