Friday, January 17, 2020

Fetal Bovine Serum-More Data

Primary and Stem Cell Culture
This just came across our radar.
"SUPPLEMENTARY MATERIAL An eye opener in stroke: Mitochondrial dysfunction and stem cell repair in MCAO induced retinal ischemia"

We are always delighted when researchers supplement their cell culture media with our Fetal Bovine Serum (FBS).
  • RPE Cells and MSC Culture Retinal pigmented epithelium (RPE, CRL-4000; ATCC) cells were cultured in Dulbecco’s Modified Eagle Media/F-12 (DMEM/F-12, 11320033; Gibco) containing 10% fetal bovine serum (FBS; FBS001; Neuromics) and 0.01 mg/ml hygromycin B (10687010; Gibco) in incubator (37°C humidified, with 5% CO2, 95% air). 
  • MSCs were maintained with α-MEM (12561056; Gibco) supplemented with 20% FBS (FBS001; Neuromics), 1% penicillin/streptomycin (15140122; Gibco), 1% non-essential amino acids (11140050; Gibco), 1% GlutaMax-I (35050061; Gibco) in incubator (37°C humidified, with 5% CO2, 95% air).
MSCs’ mitochondria were detected in RPE cells after OGD. MSCs’ mitochondria were separately stained with Mitotracker prior to co-culture with RPE cells. Confocal images of RPE cells with DAPI (blue), β-tubulin (red), and MSCs’ mitochondria stained with Mitotracker (green). MSCs’ mitochondria were detected within the boundaries of RPE cells. Scale bar 10 µm.

We are offering our USDA Origin FBS for $299/500 ml. through the end of January.

Saturday, January 11, 2020

Human Cancer Associated Fibroblasts

Customer Generated Data
Our Cancer Associated Fibroblasts (CAFs) are widely used and frequently published.

We always welcome customer data. We are especially pleased when the data confirms our cells are "walking and talking" as advertised. Here's a recent example, "Biomarkers expression by flow and confocal microscopy using your CAFs. As flow cytometry indicated, >92% cells were live and we showed expected signals (Cy3) from the live cells. Nuclear dye is Hoechst showed as blue." Courtesy of Jiehua Zhou, City of Hope.
We offer a wide range of human primary cells. Check them out today.

Tuesday, January 07, 2020

Neuromics Teams with Biowest USA

Pure and Potent Chicken Serum
We have a new partnership with the source for much of the Animal Sera provided worldwide today. They are recognized as being one of the best of the best.

I am proud to announce the addition of their Chicken Serum. Their processing is both ISO and GMP certified. Better yet, we have an introductory pricing of $299/500 ml. for the month of January.
Chicken Primordial Germ Cells Cultured in Media Supplemented with Neuromics/Biowest USA's Chicken Serum.

We will be greatly enhancing our Sera offerings in 2020. Stay tuned.