Quality Counts
Though rare, we occasionally have clients challenge the phenotype of our 21-CFR compliant human primary cells. This challenge is usually a result of certain markers not being expressed when they run immuno-fluorescence assays.
When this happens, we too test the cells in question using the antibodies against the markers in question. We have a world class team doing immuno-fluorescence assays.
Recently a customer claimed our schwann cells were negative for GFAP and P0. Here're our testing results.
Though rare, we occasionally have clients challenge the phenotype of our 21-CFR compliant human primary cells. This challenge is usually a result of certain markers not being expressed when they run immuno-fluorescence assays.
When this happens, we too test the cells in question using the antibodies against the markers in question. We have a world class team doing immuno-fluorescence assays.
Recently a customer claimed our schwann cells were negative for GFAP and P0. Here're our testing results.
For P0 (CH23009) we used green fluorescence +DAPI.
For GFAP (RA22101) we used red fluorescence + DAPI (blue)
counterstain
We take these challenges for seriously and will always test the same lot of cells internally to make sure they are "walking and talking" as advertised.
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