Friday, October 25, 2019

FBS-We're Back

Building Inventory
The supply of Fetal Bovine Serum continues to tighten. We are now on the brighter side of this after obtaining a healthy supply.

The good news is we are able to hold our pricing at $379/500 ml.
Our clients are finding it works for many different types of cells. Check out data.  We are confident you will be delighted with results.

Tuesday, October 15, 2019

Taste and AgRP

How Hunger Impacts Taste

Nature Communications just released a publication featuring use of our Agouti-Related Protein (AgRP) Antibody.

It examines the neuronal mechanisms regulating hunger-induced taste modification. Starved mice exhibit an increased preference for sweetness and tolerance for aversive taste. This hunger-induced taste modification is recapitulated by selective activation of orexigenic Agouti-related peptide (AgRP)-expressing neurons in the hypothalamus projecting to the lateral hypothalamus.
Ou Fu, Yuu Iwai, Masataka Narukawa, Ayako W. Ishikawa, Kentaro K. Ishii, Ken Murata, Yumiko Yoshimura, Kazushige Touhara, Takumi Misaka, Yasuhiko Minokoshi & Ken-ichiro Nakajima (2019). Hypothalamic neuronal circuits regulating hunger-induced taste modification. Nature Communications volume 10, Article number: 4560 https://doi.org/10.1038/s41467-019-12478-x

Chemogenetic activation of AgRP neurons induces changes in taste preference. a Schematic image of the brief access taste test. The number of licks is measured during 10 s from the first lick. b, c Sweet (b) or bitter (c) taste preferences in fed or fasted mice. Sucrose or denatonium–sucrose solutions were presented to fed or 23-h-fasted C57BL/6J WT mice. n = 6, F = 17.81, and P = 9.4 × 10–5 in b and n = 6, F = 4.14, and P = 0.045 in c, two-way ANOVA with Bonferroni post hoc test. d Bilateral injection of AAV encoding Cre-dependent hM3Dq-mCherry or hM4Di-mCherry into the arcuate nucleus (ARC) of AgRP-ires-Cre mouse. e Representative image showing hM3Dq-mCherry-expressing AgRP neurons (left) in the AgRP-hM3Dq mouse and hM4Di-mCherry-expressing AgRP neurons (right) in the AgRP-hM4Di mouse. f Chemogenetic activation of AgRP neurons led to acute food intake in AgRP-hM3Dq mice during the light period. n = 6, paired Student’s t test. g, h Brief access taste tests for sweet (g) or bitter (h) measured in AgRP-hM3Dq mice treated with saline or CNO (1.0 mg/kg i.p.) during the light cycle. n = 6, F = 8.783, and P = 0.0045 in g and n = 6, F = 7.929, and P = 0.0064 in h, two-way ANOVA with Bonferroni post hoc test. i Chemogenetic inhibition of AgRP neurons led to a reduction of food intake in AgRP-hM4Di mice during the dark cycle. n = 7, paired Student’s t test. j, k Brief access taste tests for sweet (j) or bitter (k) measured in AgRP-hM4Di mice treated with saline or CNO (1.0 mg/kg i.p.) during the dark cycle. n = 7, F = 4.748, and P = 0.032 in j and n = 7, F = 4.761, and P = 0.032 in k, two-way ANOVA with Bonferroni post hoc test. The experiments were carried out with 8- to 16-week-old male mice.
We have an extensive catalog of Neuronal Receptor Antibodies. Check the out today

Wednesday, October 02, 2019

Primary Human Cells Data

Internal Testing

We are data hounds. This is especially true when customers generate results that conflict with ours. For example, if our Human Primary Cells, in the hands of our customers, stain negative for key markers, we always retest using a 3rd Party Lab. Here're recent results for our Human Schwann Cells.
Neuromics' Schwann Cells stained with O1 Antibody (red) and DAPI (blue). 

Neuromics' Schwann Cells stained with s100 antibody (red) and DAPI (blue).
It is imperative that all our cells work as advertised. If your results do not meet expectations, we will run similar tests to make sure they walk and talk as they should.