Making Gains on Pain Research

Neuromics' Pain Research Customers continue to make gains using our Pain and Inflammation Research Antibodies and Transfection Kits. Here are the latest pubs:

Hua Zhang and A. S. Verkman. Aquaporin-1 Tunes Pain Perception by Interaction with Nav1.8 Na+ Channels in Dorsal Root Ganglion Neurons. February 19, 2010 The Journal of Biological Chemistry, 285, 5896-5906.

...chicken anti-calcitonin gene-related peptide (CGRP; 1:500, Neuromics, Edina, MN)...

Nathaniel A. Sowa, Bonnie Taylor-Blake, and Mark J. Zylka. Ecto-5'-Nucleotidase (CD73) Inhibits Nociception by Hydrolyzing AMP to Adenosine in Nociceptive Circuits. The Journal of Neuroscience, February 10, 2010, 30(6):2235-2244; doi:10.1523/JNEUROSCI.5324-09.2010.

...rabbit anti-P2X3-RA10109, Neuromics; 1:750), rabbit anti- VR1 C-Terminus (TRPV1) - mouse specific (RA14113, Neuromics; 1:750

Images: Images: Confocal images showing the effect of RTX on mu opioid receptor and TRPV1 immunoreactive DRG neurons and afferent terminals in the spinal cord. A: representative confocal images showing mu opioid receptor (green) and TRPV1 (red) immunoreactivities in DRG neurons of one vehicle- and one RTX-treated rat. Scale bar, 40 um. B: confocal images showing mu opioid receptor (green) and TRPV1 (red) immunoreactivities in afferent terminals in the spinal dorsal horn of 1 vehicle- and 1 RTX-treated rat. Scale bar, 80 um. Inset: high-magnification images (scale bar = 5 um) showing co-localization of mu opioid receptor and TRPV1 immunoreactivity in the lamina I. Co-localization of the mu opioid receptor and TRPV1 immunoreactivity is indicated in yellow when 2 images are digitally merged. All images are single confocal optical sections. Shao-Rui Chen and Hui-Lin Pan. Loss of TRPV1-Expressing Sensory Neurons Reduces Spinal mu-Opioid Receptors But Paradoxically Potentiates Opioid Analgesia. doi:10.1152/jn.01343.2005.

Neuromics' i-Fect ™ siRNA Transfection Reagent continues to be used a tool for studying expression of genes suspected to paly a role in pain . Expression studies include: DOR , The β3 subunit of the Na+,K+-ATPase, rSNSR1, NTS1. NAV1.8, Ca_V1.2 and more.

Here's a link to all transfection publications: Transfection Kit Pubs

Related Reagents:

Neurotransmission Research Antibodies-GPCRs, Ligand Gated Ion Channels, Biogenic Amines and more Opioid Neuropeptides

Purinergic Receptors Primary Neurons and Astrocytes- Primary human, rat and mouse neurons and astrocytes by Category

Opioid Receptors TRPV (Vanilloid); TRPM; TRPA and TRPCs

Turning Placenta Into Brain

We are pleased to feature a recent publication featuring:

C. Bettina Portmann-Lanz PhD, Andreina Schoeberlein PhD, Reto Portmann PhD, Stefan Mohr MD, Pierre Rollini PhD, Ruth Sager1 and Daniel V. Surbek MD. Turning placenta into brain: placental mesenchymal stem cells differentiate into neurons and oligodendrocytes. doi:10.1016/j.ajog.2009.10.893... β-Tubulin III (Tuj-1), Mouse, Neuromics...

The researchers successfully induced neural stem (NSC) and progenitor cells (NPC) from human placental tissues.

Here are the highlights:

Study Design
Placental stem cells from first-trimester placental chorionic villi and term chorion were isolated. Neural differentiation was initiated with plating on collagen, retinoic acid, and/or human brain-derived neurotrophic factor and epidermal and fibroblast growth factor. Differentiation into neurons, oligodendrocytes, and astrocytes was monitored by immunohistochemistry. Two-dimensional polyacrylamide gel electrophoresis, high-performance liquid chromatography, and tandem mass spectrometry were used to identify proteins involved in the differentiation.

Results
Differentiated cells were mostly immediately postmitotic with some more but not fully mature postmitotic neurons. Neurons had dopaminergic or serotonergic character. Some cells differentiated into predominantly immature oligodendrocytes. Upon differentiation, neuron-specific proteins were up-regulated, whereas placental proteins were reduced.

Conclusion
Stem cells derived from human placenta can be differentiated into neural progenitors.

Featured Antibody
Tuj 1 (Neuron-specific class III beta-tubulin)-Mouse

Related Reagents:
NSE (Neuron-Specific Enolase)

Nestin

Musashi-1

Neuron/Glial Markers

Stem Cell Research Reagents

Nucleostemin-Stem Cell Marker

Our Nucleostemin Antibody has proven a versatile Stem Cell Marker.

Researchers have referenced use of this antibody as a marker for Neural Progenitors and Muscle-Derived Stem Cells. In the most recent of our Nucleostemin Publications, Dr James Wang (University of Pittsburgh) references using the antibody for staining Tendon Progenitors/Stem Cells (TSCs):

Jianying Zhang and James H-C. Wang. Characterization of differential properties of rabbit tendon stem cells and tenocytes. BMC Musculoskeletal Disorders. 2010, 11:10doi:10.1186/1471-2474-11-10.
...The staining protocol used goat anti-human Nucleostemin Antibody (1:300; Neuromics, Cat. No. GT15050) and Cy3-conjugated donkey anti-goat IgG secondary antibody...

Image: Achilles Tendon Stem Cells (ATSCs) expressed nucleostemin. Insets show enlarged view of expressed nucleostemin in pink (bar: 50 μm).

Related Reagents:
Stem Cell Markers
All Stem Cell Research Reagents
Primary Neurons and Astrocytes-Primary human, rat and mouse neurons and astrocytes

i-Brite Plus!

We are all for reagents that brighten your day.

i-BRITE Plus is a glycerol based liquid. It can be used to stain cells and can be easily added to wells. It also will not shrink tissue. In addition standard to IHC/IF applications, it can be used to visualize GFP transfections and more.

Here's a publication referencing it: Ajay S. Yekkirala, Alexander E. Kalyuzhny and Philip S. Portoghese. Standard Opioid Agonists Activate Heteromeric Opioid Receptors: Evidence for Morphine and [d-Ala2-MePhe4-Glyol5]Enkephalin as Selective μ−δ Agonists. ACS Chem. Neurosci., Article ASAP DOI: 10.1021/cn9000236. Publication Date (Web): November 25, 2009. Copyright © 2009 American Chemical Society.


After that cells were washed in PBS (3 × 15 min), counterstained with DAPI and mounted under coverslips with antifade mounting media iBright Plus (cat. no. SF40000-10; Neuromics, Inc.). Images of labeled cells were collected using Olympus FluoView1000 confocal microscope.
Images: High-magnification confocal images of double-labeling immunofluorescence for HA-δ and FLAG-μ opioid receptors. HEK-293 cells stably expressing both HA-δ and FLAG-μ opioid receptors are shown labeled for μ (A, green fluorescence) and for δ (B, red fluorescence). DAPI (blue fluorescence) has been used to stain the nuclei.

Tuj-1-Neuronal Differentiation Marker

Our Tuj-1 antibodies are widely used and frequently referenced in customer publications. They are proven markers for Neural Progenitor and Neuronal Differentiation. Here's the latest reference:

Alonso M. Higuero, Lucía Sánchez-Ruiloba1, Laura E. Doglio, Francisco Portillo1, José Abad- Rodríguez, Carlos G. Dotti and Teresa Iglesias. Kidins220/ARMS modulates the activity of microtubule-regulating proteins and controls neuronal polarity and development. JBC Papers in Press. Published on November 10, 2009 as Manuscript M109.024703.

...Tuj 1 (Neuron-specific class III beta-tubulin)-Mouse (MO15013, Neuromics Antibodies, Edina, MN)...

Immunofluorescence Method:

Cells grown on coverslips were fixed for 5 min in 4% paraformaldehydecontaining 4% sucrose in phosphate buffer saline (PBS) at 37ºC. Cells were then permeabilized with 0.2% Triton X-100 in PBS during 5 min at room temperature. After blocking (5% bovine serum albumin in PBS for 1 h), cells were incubated with the corresponding primary antibodies, and immunoreactivity was detected with the suitable fluorophore-conjugated secondary antibody before mounting on slides with Mowiol4-88 (Harland Co., UK). Confocal images were acquired using an inverted Leica TCS SP5 laser confocal microscope with a 63X Plan- Achromatic oil immersion objective and processed with LAS AF Leica Application Suite and Adobe Photoshop CS2 (Adobe Systems
Inc., CA). All images correspond to the projection of sections from a ~50μm z-stack, except for colocalization analysis where they correspond to 0.5-0.7μm single sections.

Image: Rat hippocampal neurons were fixed at 1.5 DIV and immunostained for the neuronal marker βIII-Tubulin/Tuj1 (blue).

Opioid Receptors and Depression

Tricyclic antidepressants (TCAs) have been reported to interact with the Opioid Receptor system, but their pharmacological activity at opioid receptors has not yet been elucidated.

We would like to share a recent publications that sheds more light on the mystery. Our purified rabbit ployclonal phosphoERK1/2 Antibody is also referenced.

Pierluigi Onali, Simona Dedoni and Maria C. Olianas. Direct Agonist Activity of Tricyclic Antidepressants at Distinct Opioid Receptor Subtypes. JPET January 2010 vol. 332 no. 1 255-265 .

At the cloned μ-opioid receptor, TCAs showed low affinity and no significant agonist activity. These results show that TCAs differentially regulate opioid receptors with a preferential agonist activity on either δ or κ subtypes and suggest that this property may contribute to their therapeutic and/or side effects.

Related Reagents:
phosphoERK1/2 (Rabbit MAb)
Immune Response Research Antibodies
Immune Response Research Proteins
Neurotrophins and Growth Factor Antibdodies
Neurotrophin Proteins
Pain and Inflammation Research Antibodies

Hope for Stroke Victims-Transplanting STEMEZ hNP1 Cells

In a recent publication (Jen et al., 2009) Neuromics'/ArunA’s STEMEZ^TM human neural progenitor (hNP1) cells when injected (sterotaxic) into a rat stroke model produced significant beneficial results. The hNP1 cells reduced the infarct area by 50% and were positive for neuronal marker proteins, cleaved caspase-3 and 40% of the cells showed spontaneous action potentials and excitatory postsynaptic currents measured by patch clamp recordings at 8 weeks post hNP1 cell injections. The treated rats had improves cognitive and sensorimotor functions between four to nine months post injection. These Sprague–Dawley rats were not immunosuppressed.

Kunlin Jin, XiaoOu Mao, Lin Xie, Veronica Galvan, Bin Lai, Yaoming Wang, Olivia Gorostiza, Xiaomei Wang and David A Greenberg. Transplantation of human neural precursor cells in Matrigel scaffolding improves outcome from focal cerebral ischemia after delayed postischemic treatment in rats. Journal of Cerebral Blood Flow & Metabolism advance online publication 14 October 2009; doi: 10.1038/jcbfm.2009.219.

Featuring:
STEMEZ(TM) hNP1 Human Neural Progenitors Discovery Kit
Other Reagents:
STEMEZ(TM) hN2 Human Neurons Discovery Kit
All Stem Cell Research Reagents
Primary Neurons and Astrocytes

New Mouse Monoclonal GFAP Antibody

Check it out!

GFAP Antibody

Excelent marker for human astrocyte intermediate filaments in the central nervous system. It has also been detected in the glial cells of the enteric nervous system and some Schwann cells in the peripheral nervous systems.

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Blood Pressure, Transient Receptor Potential Vanilloid 1 Receptors and Baroreceptors

Dr. Dr. Hui-Lin Pan (Department of Anesthesiology) and his team at M. D. Anderson Cancer Center has been a loyal user of our TRPV1 (VR1) since the early days of Neuromics' existence. We appreciate their continuing to use our reagent in their research.

Here's yet another publication:

Hao Sun, De-Pei Li, Shao-Rui Chen, Walter N. Hittelman and Hui-Lin Pan. Sensing of Blood Pressure Increase by Transient Receptor Potential Vanilloid 1 Receptors on Baroreceptors. doi:10.1124/jpet.109.160473

...VR1 C-terminus (TRPV1), dilution 1:1000, Neuromics...

Related Reagents:
All TRP Antibodies
Pain and Inflammation Research Antibodies
Neurotransmission -Neurotransmission Research Antibody Categories

NPY Y2R and IHC-Mouse Distal Colon

Lixin Wang, Guillaume Gourcerol, Pu-Qing Yuan, S. Vincent Wu, Mulugeta Million, Muriel Larauche, and Yvette Taché. Peripheral peptide YY inhibits propulsive colonic motor function through Y2 receptor in conscious mice. Am J Physiol Gastrointest Liver Physiol (November 5, 2009). doi:10.1152/ajpgi.00349.2009.
...Note: Excellent IHC staining of myenteric plexus and submucosal (mouse distal colon) tissue-Free floating submucosal and LMMP whole mounts of both proximal and distal colon from 3 naïve mice were treated in 10% normal goat serum each for 30 min, and followed by incubation with polyclonal rabbit anti- NPY Y2 Receptor diluted at 1:1,000 (Neuromics, Inc., Edina, MN)...

Related Antibodies to Consider:
NPY Y2 Receptor-C/N Terminus
NPY Y1 Receptor
ppNPY
All Neuropeptide and Neuropeptide Receptor Antibodies
Pain and Inflammation Research Antibodies
Diabetes and Obesity Research Antibodies

More Pubs Referencing Neuromics' mGluRs

Jakob S. Satz, Alisdair R. Philp, Huy Nguyen, Hajime Kusano, Jane Lee, Rolf Turk, Megan J. Riker, Jasmine Hernández, Robert M. Weiss, Michael G. Anderson, Robert F. Mullins, Steven A. Moore, Edwin M. Stone, and Kevin P. Campbell. Visual Impairment in the Absence of Dystroglycan. J. Neurosci., Oct 2009; 29: 13136 - 13146 ; doi:10.1523/JNEUROSCI.0474-09.2009.
....anti-mGluR6 (Neuromics)...

Lasani S. Wijetunge, Sally M. Till, Thomas H. Gillingwater, Cali A. Ingham, and Peter C. Kind. mGluR5 Regulates Glutamate-Dependent Development of the Mouse Somatosensory Cortex. The Journal of Neuroscience, December 3, 2008, 28(49):13028-13037; doi:10.1523/JNEUROSCI.2600-08.2008.
...Western blotting was performed as mentioned above and membranes were probed with antibodies against mGluR5 (1:4000, Neuromics)...
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gp130, IL-6 and Expression and Neuropathic Pain

We wanted to post yet another publication referencing use of one of our Pain and Inflammation Research Antibodies. Here the researchers used our Mouse Monoclonal gp130/CD130 for Immunohistochemistry.

Manfred Andratsch, Norbert Mair, Cristina E. Constantin, Nadja Scherbakov, Camilla Benetti, Serena Quarta, Christian Vogl, Claudia A. Sailer, Nurcan Üceyler, Johannes Brockhaus, Rudolf Martini, Claudia Sommer, Hanns Ulrich Zeilhofer, Werner Müller, Rohini Kuner, John B. Davis, Stefan Rose-John, and Michaela Kress. A Key Role for gp130 Expressed on Peripheral Sensory Nerves in Pathological Pain. J. Neurosci., Oct 2009; 29: 13473 - 13483 ; doi:10.1523/JNEUROSCI.1822-09.2009

The results suggest that gp130 expressed in sensory nerves not only mediates chronic inflammatory pain, but also contributes significantly to complex interactions between immune cells, tumor cells, and nerves in the context of cancer-evoked pain. Moreover, we identify IL-6 activating gp130, Gab1/Gab2, PI3K, and PKC- and regulating TRPV1 as a key mechanism linking cytokine release to sensitization of pain-sensing nerves.

Related Links
Immune Response
Opioid Neuropeptides
Opioid Receptors
Neurotransmissiom Research Antibodies
GPCRs, Ligand Gated Ion Channels, Biogenic Amines and more
Purinergic Receptors
TRPV (Vanilloid); TRPM; TRPA and TRPCs
i-Fect Transfection Kit
gene silencing of DOR, NaV1.8 tetrodotoxin-resistant sodium channel, NTS2 and more in-vitro and in vivo
Primary Neurons and Astrocytes
Primary human, rat and mouse neurons and astrocytes by Category

Reagents that work for Drug Discovery and Basic Research.

We have redesigned our website! New capabilities easier for visitors to find products and related product content:

Reagents that work for Drug Discovery and Basic Research.

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NSE and TUJ-1 and Parkinson's Disease Research

I would like to thank Meghan Coakley from University College Cork for alerting me to a new publication referencing our Chicken NSE (Neuron-Specific Enolase) and Tuj 1 (Neuron-specific class III beta-tubulin) antibodies.

Here's her feedback: "Just letting you know we published our paper using the beta-III-Tubulin and NSE antibodies you supplied to us – Timmons et al., Neuroscience Letters, Oct 1, 2009 [Epub ahead of print]. The antibodies were excellent and I’m sure we’ll be using Neuromics again in the future."

Timmons S, Coakley MF, Moloney AM, O' Neill C. Akt signal transduction dysfunction in Parkinson's disease. Neurosci Lett. 2009 Oct 1. [Epub ahead of print].

Abstract: Significant attention has been drawn to the potential role of defective PI3-kinase-Akt (PKB) signalling in Parkinson's disease (PD) neurodegeneration and to the possibility that activation of Akt may provide neuroprotection in PD. However, little knowledge exists on the integrity of the Akt system in PD. Results of the present study show diminished levels of both total and active phospho(Ser473)-Akt in the brain in PD. This was evident by western blot analysis of midbrain fractions from PD compared to non-PD control brain, but more specifically by immunofluorescence microscopy of the substantia nigra pars compacta (SNpc). Here, double immunofluorescence microscopy found Akt and phospho(Ser473)-Akt to be expressed at high levels in tyrosine hydroxylase (TH) immunopositive dopaminergic neurons in control human brain. Selective loss of these neurons was accompanied by a marked decrease of Akt and phospho(Ser473)-Akt expression in the PD brain, however Akt and active phospho(Ser473)-Akt are still evident in degenerating dopaminergic neurons in the disease. This suggests that it may be possible to target neuronal Akt in advanced PD. Converse to the marked loss of neuronal Akt in PD, increased Akt and phospho(Ser473)-Akt levels were observed in small non-TH positive cells in PD SNpc, whose increased number and small nuclear size indicate they are glia. These findings implicate defective Akt as a putative signalling pathway linked to loss of dopaminergic neurons in PD.

Other Reagents to Consider:
Tuj 1 (Neuron-specific class III beta-tubulin)-Mouse Monoclonal
Neuron/Glial Marker Antibodies
Neurotrophins-Neuron/Glial Marker Proteins
Neurodegenerative Disease Research Antibodies
Neurodegenerative Disease Research Proteins
Stem Cell Research Reagents

More Success with TRPV1

Dr. Irmgard Tegeder and team have been using our VR1 N-Terminus (TRPV1) as a marker to study how B kinase (IKK) plays a role in sensory neuron excitability and nociception.

The antibody was used for both Western Blot and Immunohistochemistry. Here's a recent publication of their results:

Vanessa Bockhart, Cristina Elena Constantin, Annett Häussler, Nina Wijnvoord, Maike Kanngiesser, Thekla Myrczek, Geethanjali Pickert,Laura Popp, Jürgen-Markus Sobotzik, Manolis Pasparakis, Rohini Kuner, Gerd Geisslinger, Christian Schultz, Michaela Kress, and Irmgard Tegeder. Inhibitor B Kinase β Deficiency in Primary Nociceptive Neurons Increases TRP Channel Sensitivity. The Journal of Neuroscience, October 14, 2009, 29(41):12919-12929; doi:10.1523/JNEUROSCI.1496-09.2009

Related Reagents:
All TRPV (Vanilloid); TRPM; TRPA and TRPC Antibodies
Pain and Inflammation Research Antibodies

MMP-9 Squared

We are feverishly working on adding reagents for studying Autoimmunity and Immune Response. In this context, we wanted to alert you to 2 new publications referencing our Matrix Metalloproteinase 9 (MMP-9) antibody.

Zhi-Yuan Zhang, Zhiren Zhang, Caroline Zug, Barbara Nuesslein-Hildesheim, David Leppert and Hermann J. Schluesener. AUY954, a selective S1P1 modulator, prevents experimental autoimmune neuritis. doi:10.1016/j.jneuroim.2009.09.010.

Abstract:
Experimental autoimmune neuritis (EAN) is a T cell-mediated autoimmune inflammatory demyelinating disease of the peripheral nervous system and an animal model of human inflammatory demyelinating polyradiculoneuropathy. AUY954, which targets selectively the sphingosine-1-phosphate receptor 1 (S1P1), is known to sequester lymphocytes into secondary lymphoid tissues. In EAN rats, AUY954 greatly prevented paraparesis if administrated from the day of immunization. T cell, B cell, and macrophage infiltration, inflammatory demyelination, and local expression of interleukine-17 and matrix metalloproteinase-9 in sciatic nerves of EAN rats were significantly decreased by AUY954 treatment. Therefore, S1P1 modulation might be a potential treatment option for inflammatory neuropathies.

Image: MMP-9 expression in the sciatic nerves of control (A) vs AUY954 (B) treated rats.

Yasuki Yasuda, Yoko Matsumura, Kazuki Kasahara, Noriko Ouji, Shigeki Sugiura, Keiichi Mikasa, and Eiji Kita. Microbial exposure early in life regulates airway inflammation in mice after infection with Streptococcus pneumoniae with enhancement of local resistance. Am J Physiol Lung Cell Mol Physiol, Sep 2009; doi:10.1152/ajplung.00193.2009

...with 10% milk in TBST (10mM Tris, 0.15M NaCl, 0.1% Tween-20), followed by probing with goat anti-mouse MMP-9 antibodies (Ab) (Neuromics Antibodies, Edina, MN, GT15020; 1:5000)...

Related Reagents:

CaMKIIs
CD and Cell Surface Markers
ERKs/MAPKs
Heat Shock Proteins-New
Matrix Metalloproteinases (MMPs)
ILs, CCRs, CXCs and STATs
Wnts/FZDs
Other Immune Response Antibodies

Immune Response Research Proteins

Apoptosis Research Reagents

Rock Solid Tuj-1

The feedback from customers on our Tuj 1 (Neuron-specific class III beta-tubulin) is that it works!

This is confirmed by the growing list of references in key publications. Here's the latest:

S A Sakowski, S B Heavener, J S Lunn, K Fung, S S Oh, S K Spratt, N D Hogikyan and E L Feldman. Neuroprotection using gene therapy to induce vascular endothelial growth factor-A expression. Gene Therapy advance online publication 3 September 2009; doi: 10.1038/gt.2009.111.

...TUJ1 (Neuromics, Edina, MN, USA). ...

Tuj 1 (Neuron-specific class III beta-tubulin)
Related Reagents:
Nestin
Musashi-1
Other Reagents to Consider:
Stem Cell Reagents
Neuron/Glial Markers

Prodynorphin at Work

We would like to Dr. Andrew Todd for sharing this excellent IHC image using our Guinea Pig ProDynorphin (rat) antibody.

Image: Staining of adult rat spinal cord.

The tissue is perfusion-fixed (4% freshly prepared formaldehyde) adult rat spinal cord, reacted overnight with the PPD at 1:1000 and then o/n in Alexa488 secondary (raised in donkey, Invitrogen, 1:500).

The confocal image stack was taken through a 60x oil lens (Bio-Rad Radiance confocal) - pixel size is 0.196 micrometre and this is a projection of 10 confocal optical sections at 0.5 micrometre z-spacing.
Customer Publications
Related Reagents:
proDynorphin (guinea pig)
Opioid Receptors
Pain and Inflammation Antibodies

CAMKIIs and Neurotransmitters.

Here's an update on recent developments. First, we wanted to share a recent publication referencing 2 of our Neurotransmission Research Antibodies:

Haifeng Zhaoa, Qiong Li, Xinrong Pei, Zhaofeng Zhang, Ruiyue Yang, Junbo Wang and Yong Li. Long-term ginsenoside administration prevents memory impairment in aged C57BL/6J mice by up-regulating the synaptic plasticity-related proteins in hippocampus. Behavioural Brain Research Volume 201, Issue 2, 12 August 2009, Pages 311-317. doi:10.1016/j.bbr.2009.03.002. ...anti-phospho-CaMKII (Thr286) and anti- CaMKII (Neuromics, USA)...

Second, we continue to bulk up on reagents for Neurotransmission, Neuromodulation and Synaptic Plasticity Research Reagents. Check out these product categories:

Biogenic Amines
Calcium Signaling
G-Protein Coupled Receptors
Ligand-gated Ion Channels
Transient Receptor Potential Channels
Other Neurotransmitters

Primary Rat, Human and Mouse Neurons and Astrocytes

TRPV1 Antibodies in Action

We would like to thank Dr. Fletcher White, LUMC, for Sharing this data with us:

Images: The CCR2 chemokine receptor colocalized with IB4 and TRPV1, markers of nociceptive neurons, after injury. A) Many lumbar DRG neurons in vehicle-treated rat sensory neurons were positive for IB4, a neuronal phenotype that distinguishes some C-fiber nociceptors (red cells), however there was no expression of the CCR2 protein. B) After perineural gp120/hCD4 treatment, CCR2 protein expression (green cells) was upregulated, and co-localized with IB4. C) Both gp120/hCD4 and ddC treatment resulted in an upregulation of CCR2 expression (green cells) in many small and medium diameter neurons. Again, CCR2 co-localized in a number of IB4 positive cells. D) The TRPV1 channel is present on many nociceptive neurons and is involved in the neuropathic pain mechanism. Under normal conditions, TRPV1 was expressed in neurons (red cells). E) After gp120/hCD4 treatment, CCR2 expression is upregulated (green cells) and colocalized with TRPV1. F) After the combination of gp120/hCD4 and ddC treatments, again CCR2 was upregulated to a similar degree as gp120/hCD4 treatment alone and exhibited some colocalizations with TRPV1.
Scale Bar: 100um.

We would also like to share recent publications referencing our TRPVs:

Hao Sun, De-Pei Li, Shao-Rui Chen, Walter Hittelman, and Hui-Lin PanSensing of Blood Pressure Increase by Transient Receptor Potential Vanilloid 1 Receptors on BaroreceptorsJ. Pharmacol. Exp. Ther., Sep 2009; doi:10.1124/jpet.109.160473 ......guinea pig anti-TRPV1, dilution 1:1000, Neuromics, Minneapolis, MN) and secondary antibody...guinea pig anti-VR1 C-terminus (TRPV1), dilution 1:1000,Neuromics; and rabbit anti-NF200, dilution 1:100...guinea pig anti-TRPV1, dilution 1:1000, Neuromics) for 2 hr at room temperature and

overnight......Kenjiro Matsumoto, Emi Kurosawa, Hiroyuki Terui, Takuji Hosoya, Kimihito Tashima, Toshihiko Murayama, John V. Priestley, and Syunji HorieLocalization of TRPV1 and contractile effect of capsaicin in mouse large intestine: high abundance and sensitivity in rectum and distal colonAm J Physiol Gastrointest Liver Physiol, Aug 2009; 297: G348 - G360. ......TRPV1 antibody (mouse TRPV1 C-terminus; Neuromics, Minneapolis, MN) were 1:60,000 for rectum...different anti-TRPV1 antibodies (1:60,000, Neuromics, and rat TRPV1 COOH-terminus, 1:1,000...experiments, the antibody (1:60,000; Neuromics) was preincubated with 10 uM of the corresponding......

Featured Reagents:
VR1 N-Terminus (TRPV1)
VR1 C-terminus (TRPV1)
VR1 C-Terminus (TRPV1) - mouse specific
Related Reagents
VR1 (TRPV1)-Goat
VR like-3 (TRPV3)
All TRPV (Vanilloid); TRPM; TRPA and TRPCs
Pain and Inflammation Antibodies