Cancer Associated Fibroblasts (CAFS) Traction Dynamics and Remodeling in 3D Matrices

 Neuromics' Colorectal CAFS in Action

In a first of its kind study, the mechanical properties of our CAFS were determined. This is important because, understanding how these cell behave in vivo will help improve the efficacy of new therapies: Bashar Emon, Zhengwei Li, Md Saddam Hossain Joy, Umnia Doha, Farhad Kosari, and M Taher A Saif. (2020). A Novel Method for Sensor-Based Quantification of Single/Multi-Cellular Traction Dynamics and Remodeling in 3D Matrices. bioRxiv. doi: 10.1101/2020.09.24.311647

"Cells in vivo generate mechanical forces (traction) on surrounding 3D extra cellular matrix (ECM) and cells. Such traction and biochemical cues may remodel the matrix, e.g. increase stiffness, which in turn influences cell functions and forces. This dynamic reciprocity mediates development and tumorigenesis. Currently, there is no method available to directly quantify single cell traction and matrix remodeling in 3D. Here, we introduce a method to fulfil this long-standing need. We developed a high-resolution microfabricated sensor which hosts a 3D cell-ECM tissue formed by self-assembly. It measures cell forces and tissue-stiffness and can apply mechanical stimulation to the tissue. We measured single and multicellular force dynamics of fibroblasts (3T3), human colon (FET) and lung (A549) cancer cells and cancer associated fibroblasts (CAF05) with 1 nN resolution. Single cells show significant force fluctuations in 3D. FET/CAF co-culture system, mimicking cancer tumor microenvironment, increased tissue stiffness by 3 times within 24 hours."

Here's one of 10 videos illustrating these properties.

 CAFS are widely used and frequently published. Check them out today!

Neuromics' n-Fect Delivers

 Gene Expression Tools in Action

Neuromics has a wide offering of transfection reagents that are perfect for both in vivo and in vitro applications. We take pride in seeing our product used in research, which is certainly true of our transfection reagents (see here).

Furthermore, we'd like to highlight a recent use of our n-Fect Transfection Kit (cat.# NF30150) in a doctoral thesis recently published. The paper looked into the neuroscience of alcohol addiction, looking towards the purinergic family receptor P2X4, which has been linked to alcohol addiction in mouse models. Using our n-Fect Transfection Kit, the molecular and cellular mechanisms linking P2X4 expression and voluntary alcohol consumption are explored. Our n-Fect reagents were used on brain slices containing the ventral tegmental area (VTA) of mice. They conclude that purinergic control of VTA neurons is a neurotransmitter system that should be further studied as a target for alcohol dependence drugs.

Image: Examples of p-Fect (Cat.# PF3000) transfection in different cell types.

Questions? Do not hesitate to contact Rose Ludescher, VP of customer satisfaction -rose@neuromics.com

Premium Virus Transport Media vs PBS

 There is a difference

Here's a video that illustrates the difference between CDC recommended Virus Transport Media and Sterile PBS (salt water). It answers the questions "what is in your VTM" and "why does it matter". Please consider sharing so it can find its way to those in need!

Check out our VTM-https://www.neuromics.com/virus-transport-media

METHOD OF TREATING CENTRAL NERVOUS SYSTEM DISEASE

 Blood-Brain Barrier (BBB) Permeability Assay

Yes, These are troubled times. With our current focus on eradicating COVID-19, we forget that many of the world's most insidious and costly diseases have limited treatments and no cures.

That is why Neuromics continues to develop its human based 3-D Cell-Based Assays. At the center if our offerings is our BBB Model.

Here's a recent patent application that includes a permeability assay using our model-Sookhee Bang, Jeong Kuen Song, Seung-wook Shin, Kwan Hee Lee, and Ho June Lee. (2020). Method of Treating Central Nervous System Disease. United States Patent Application 20200230218

BBB Permeability Assay of AL04 The in vitro human BBB model (Neuromics, USA) was established using co-cultures of primary Human brain endothelial cells (HBEC), Human brain pericytes (HBPC), and Human brain astrocytes (HBAC). In vitro human BBB model kit has two sides (luminal, blood/abluminal, brain) with 12 transwell inserts (polyester membrane, 0.4 um pore, diameter 12 mm, insert growth area: 1.12 cm2). HBPC were grown on the bottom side of the inserts, HBEC were monolayered on the upper side of the inserts, and HBAC were grown on the bottom of the 12-well culture plate (Neuromics, USA). In vitro BBB model was activated according to the manufacturer's instructions for 4 days. Briefly, the medium from both luminal and abluminal (lower, brain) sides of the transwell insert was changed every other day. Before the transport experiment, the abluminal side was filled with the permeability assay medium. For the permeability assay, purified AL04 or recombinant HSA (Sigma, USA) were added to the luminal side (0.3 ml) of the transwell insert to yield a final concentration of 1 or 10 uM. Incubations were performed on orbital shaker (100 rpm) at 37° C. Samples (150 μl) were collected from the abluminal side (1.2 ml) at 60, 120 and 240 min and immediately replaced with fresh permeability assay medium. The concentrations of transported AL04 or rHSA were measured by Human albumin quantitation ELISA kit (Bethyl laboratory, USA) and analyzed using the standard curve method. Permeability coefficients (Pe, cm s−1) were calculated using the equation: Pe=(VA/(A×c0))×(dQ/dt), where VA is volume of assay buffer in blood side (inside of the insert), A is the surface area of the insert (1.12 cm2), c0 the initial concentration of protein sample added into blood side, dQ/dt the concentration of transported protein sample in brain side in a defined time period. The permeability coefficients (Pe, cm s−1) for the purified AL04 or recombinant HSA were calculated as previously described (Prades, 2015; Nakagawa, 2009).

If you have interest and want to learn more, please email Rose Ludescher, VP, Customer Satisfaction-rose@neuromics.com

Be safe; be healthy.

What's In Your Virus Transport Media (VTM)

We Have FBS in Bulk Available

Key Component of Virus Transport Media (VTM)
Neuromics is known to have some of the best Fetal Bovine Serum (FBS) for most applications. This includes being a component of VTM manufactured to the CDC's most recommended recipe.

Unlike some other suppliers, we are fully transparent as to pricing and available inventory. Check us out today.

We Have VTM in Bulk

At Capacity for VTM Based COVID-19 Sample Transport Kits
We are well on our way to delivering 100,000 Tubes + VTM to the state of Ohio. A big thank you to my  team and friends for making this happen.

5,000 Tubes+VTM Ready to Ship to Ohio

We do have a capacity to provide 4,000 Liters/Week of VTM in 1 Liter Bottles.

If you have current or future needs do not hesitate to contact me directly @ pshuster@neuromics.com or direct phone 612-801-1007. Pete Shuster, Owner, Neuromics

We Have VTM and COVID19 Test Kits

Providing Kits Coast to Coast and Points in Between

From Seattle, Washington to New York City and in between, our Virus Transport Medium has been used in COVID-19 testing. Neuromics is doing our part by helping to expand testing capacity, allowing the nation to recover from the harships associated with the pandemic.

Our VTM is manufactured using our FBS following CDC guidelines, ensuring that it is sterile and safe for testing. Additionally, each lost of VTM goes through quality control before release (sample CoA).

 We offer our VTM in many different forms, including kits (tubes and sterile swabs) and larger quantities that can be aliquoted into tubes under a sterile hood.

If you have immediate needs please email david@neuromics,com, Manager, COVID-19 Testing Supplies and kits.

We Have Virus Transport Media (VTM) in Tubes

VTM The Way You Need It
We have Virus Transport Media in tubes with 2 options.

Size	Price
VTM-1: 2 ml 15 ml tube (100 mm) - qty 15 (Available after 4/24)	$69.00
VTM-2: 2 ml 15 ml tube (120 mm) - qty 15	$74.00

We can also provide tubes with custom volumes of VTM.

Should you have questions or want to explore custom VTM volume options, please contact me directly at 612-801-1007/pshuster@neuromics.com.

Pete Shuster

CEO and Owner

Neuromics

Schwann Cell Data

Quality Counts
Though rare, we occasionally have clients challenge the phenotype of our 21-CFR compliant human primary cells. This challenge is usually a result of  certain markers not being expressed when they run immuno-fluorescence assays.

When this happens, we too test the cells in question using the antibodies against the markers in question. We have a world class team doing immuno-fluorescence assays.

Recently a customer claimed our schwann cells were negative for GFAP and P0. Here're our testing results.

For P0 (CH23009) we used green fluorescence +DAPI.

For GFAP (RA22101) we used red fluorescence + DAPI (blue) counterstain

We take these challenges for seriously and will always test the same lot of cells internally to make sure they are "walking and talking" as advertised.

We have Corona Virus Transport Media

We have Corona Virus Transport Media! We are pleased to announce we now have media designed to move COVID-19 testing samples from point A to B. It's pricing is in line with all the many media we currently provide. If you know of any hospitals, clinics or testing facilities that would benefit we all are ears-https://www.neuromics.com/VTM #covid19 #coronavirus

We Are Open

Providing Cells, Media and Supplements in the time of Covid-19
If you call us, we will answer the phone. If you submit an order, it will ship.

Neuromics stands ready to serve you.

Image: Neuromic's Human Pancreatic CAF-Stellate Cells in Culture

FBS only $299/500 ml. We have plenty of inventory so we can continue with this pricing for the duration of the Pandemic.

Stay safe.

Save Hundreds on Tet Free FBS

For Healthy and Happy Cultures
Check it out. Only $299/500 ml.
USDA Origin FBS.

Single use automated processing system from filtering to labeling.

Questions? Contact me directly @ pshuster@neuromics.com.

Pete Shuster-CEO and Owner

Customer Data Wanted

Reward is a $25 Amazon Gift Card
We are always honored when you share your data with us! To learn more check out the video.

We make it easy to share. Just email your data to rose@neuromics.com. We will email you the gift card.

Our New USDA FBS is Tet Free

Compare and Save!
In researching Neuromics' Competitors pricing on Tetracycline Free FBS, I noticed pricing ranging from $650-1,000+. Check us out. Only $299/500 ml. bottle.

FBS - Fetal Bovine Serum USDA Origin

FBS002

500 mL

$299.00

Here's our COA.

To learn more simply email rose@neuromics.com.

Fetal Bovine Serum-More Data

Primary and Stem Cell Culture
This just came across our radar.
"SUPPLEMENTARY MATERIAL An eye opener in stroke: Mitochondrial dysfunction and stem cell repair in MCAO induced retinal ischemia"

We are always delighted when researchers supplement their cell culture media with our Fetal Bovine Serum (FBS).

• RPE Cells and MSC Culture Retinal pigmented epithelium (RPE, CRL-4000; ATCC) cells were cultured in Dulbecco’s Modified Eagle Media/F-12 (DMEM/F-12, 11320033; Gibco) containing 10% fetal bovine serum (FBS; FBS001; Neuromics) and 0.01 mg/ml hygromycin B (10687010; Gibco) in incubator (37°C humidified, with 5% CO2, 95% air). 
• MSCs were maintained with α-MEM (12561056; Gibco) supplemented with 20% FBS (FBS001; Neuromics), 1% penicillin/streptomycin (15140122; Gibco), 1% non-essential amino acids (11140050; Gibco), 1% GlutaMax-I (35050061; Gibco) in incubator (37°C humidified, with 5% CO2, 95% air).

MSCs’ mitochondria were detected in RPE cells after OGD. MSCs’ mitochondria were separately stained with Mitotracker prior to co-culture with RPE cells. Confocal images of RPE cells with DAPI (blue), β-tubulin (red), and MSCs’ mitochondria stained with Mitotracker (green). MSCs’ mitochondria were detected within the boundaries of RPE cells. Scale bar 10 µm.

We are offering our USDA Origin FBS for $299/500 ml. through the end of January.

Human Cancer Associated Fibroblasts

Customer Generated Data
Our Cancer Associated Fibroblasts (CAFs) are widely used and frequently published.

We always welcome customer data. We are especially pleased when the data confirms our cells are "walking and talking" as advertised. Here's a recent example, "Biomarkers expression by flow and confocal microscopy using your CAFs. As flow cytometry indicated, >92% cells were live and we showed expected signals (Cy3) from the live cells. Nuclear dye is Hoechst showed as blue." Courtesy of Jiehua Zhou, City of Hope.

We offer a wide range of human primary cells. Check them out today.

Neuromics Teams with Biowest USA

Pure and Potent Chicken Serum
We have a new partnership with the source for much of the Animal Sera provided worldwide today. They are recognized as being one of the best of the best.

I am proud to announce the addition of their Chicken Serum. Their processing is both ISO and GMP certified. Better yet, we have an introductory pricing of $299/500 ml. for the month of January.

Chicken Primordial Germ Cells Cultured in Media Supplemented with Neuromics/Biowest USA's Chicken Serum.

We will be greatly enhancing our Sera offerings in 2020. Stay tuned.