Fetal Bovine Serum-More Data

Primary and Stem Cell Culture
This just came across our radar.
"SUPPLEMENTARY MATERIAL An eye opener in stroke: Mitochondrial dysfunction and stem cell repair in MCAO induced retinal ischemia"

We are always delighted when researchers supplement their cell culture media with our Fetal Bovine Serum (FBS).

• RPE Cells and MSC Culture Retinal pigmented epithelium (RPE, CRL-4000; ATCC) cells were cultured in Dulbecco’s Modified Eagle Media/F-12 (DMEM/F-12, 11320033; Gibco) containing 10% fetal bovine serum (FBS; FBS001; Neuromics) and 0.01 mg/ml hygromycin B (10687010; Gibco) in incubator (37°C humidified, with 5% CO2, 95% air). 
• MSCs were maintained with α-MEM (12561056; Gibco) supplemented with 20% FBS (FBS001; Neuromics), 1% penicillin/streptomycin (15140122; Gibco), 1% non-essential amino acids (11140050; Gibco), 1% GlutaMax-I (35050061; Gibco) in incubator (37°C humidified, with 5% CO2, 95% air).

MSCs’ mitochondria were detected in RPE cells after OGD. MSCs’ mitochondria were separately stained with Mitotracker prior to co-culture with RPE cells. Confocal images of RPE cells with DAPI (blue), β-tubulin (red), and MSCs’ mitochondria stained with Mitotracker (green). MSCs’ mitochondria were detected within the boundaries of RPE cells. Scale bar 10 µm.

We are offering our USDA Origin FBS for $299/500 ml. through the end of January.

Neuromics New FBS-329/500 ml.

Premium Ultra-pure FBS!
Check it out today

Ultrapure FBS is manufactured, by our OEM partner Biowest USA. in a fully integrated environment from raw material collection to finished product. Our production facility is fully certified to ISO 9000 and ISO 13485. Ultrapure means:

• Lowest endotoxin level 0,1 EU/mL. 
• Triple 0,1 μm Filtered. 
• Free of Virus and Mycoplasma. 
• Tested Ideal for Sensitive Cells and Applications. 

This FBS can be used to culture all cell types including macrophages, cancer and cancer related cells, hybridomas and more. It works great for all our human primary cells and stem cells.

Human Cells-utopia!

Great for use a controls vs Differentiated iPSCs

We have a cornucopia of Human Primary Neuron, Astrocytes and Schwann Cells plus CAFS. Featured Assays:
Human Brain Pericytes used to study Guide Axon Guidance.
Human Pancreatic CAFS and Tumor Dynamics.

Axon guidance at the site of a cervical spinal cord injury in a rat model. (Ai) Schematic illustrating transplantation of scaffold into a C-4 hemisection. The injury cavity is shown prior to (ii) and immediately following (iii) transplantation. (Bi) Scaffold conditioned with flow exhibits viable GFP-labeled microvessels (green) (ii) and alignment of host axons (magenta) infiltrating the scaffold in the rostral-caudal direction (grey arrow). (C) Scaffold conditioned in static conditions showing disrupted alignment of both microvessels (ii) and host axons (iii). (D–F) Microvessel and axon plots showing alignment (D,E) and length (F). Scale bars, 1 mm (Aii,Aiii) and 50 μm (B,C). Data are presented as mean ± s.e.m. ***P < 0.001; statistical significance was calculated using Welch Two Sample t-test. White arrows denote proximity of axons with microvessels. Microvessel alignment values (n = 30), axon alignment values (n = 30), microvessel length values (n = 15), and axon length values (n = 15) are from single hydrogel samples per condition.

Representative CAFs spheroids embedded in collagen gels at time 0 and at 6 h post implantation, respectively.

It is imperative that all our cells work as advertised. If your results do not meet expectations, we will run similar tests to make sure they walk and talk as they should.

We wish you and yours a Happy Holiday Season, Pete Shuster, CEO and Owner Direct phone: 612-801-1007 or pshuster@neuromics.com

Culturing Stem Cells in 3-D

Requires Potent Media + Supplements

Neuromics is responding to the many challenges our clients face in building 3-D, in-vivo like, cell- based assays. We do this by offering the most potent Media plus Supplements like FGFS.

Here's a protocol for single cell 3-D assays using hMSCs and Hydrogels. It features use of our ISOKine^TM FGF

Images: Cell-centering in cytocompatible microgels enables long-term single-cell 3D culture by preventing cell escape. a) Qualification of Dex-TA microgel crosslinking as a function of the microemulsion flow rate (Qemulsion) and concentration of the H2O2 feed ([H2O2]feed). Blue, green, and red indicate incomplete crosslinking, complete crosslinking, and H2O2 excess, respectively. b,c) Amplex Red assay to quantify the concentration of residual H2O2 ([H2O2]emulsion) in Dex-TA microgel precursor droplets and crosslinked microgels after their retrieval from the diffusion-based crosslinking platform. d) The microencapsulation procedure had no detrimental effect on short-term cell survival. e) Delayed crosslinking resulted in 4 ± 1% cell escape after 7 d of in vitro culture, as compared to 27 ± 5% cell escape when using coupled emulsification and gelation. f) The number of encapsulated cells per microgel tightly followed the Poisson distribution and remained similar throughout long-term (28 d) of in vitro culture, which confirmed that cell centering prevents cell escape. g–i) MSCs encapsulated in delayed enzymatically crosslinked microgels remained viable and metabolically active throughout 28 d of in vitro culture. j) Positive Oil Red O and k) Alizarin Red staining confirmed that l) more than 60% of the microencapsulated MSCs could differentiate into the adipogenic and osteogenic lineage, respectively. Black scale bars: 50 µm, white scale bars: 5 µm. DOI: 10.1002/smll.20160371.

Protocol for Cell Isolation and Expansion: Human MSCs were isolated from fresh bone marrow samples and cultured as previously described. The use of patient material was approved by the local ethical committee of the Medisch Spectrum Twente and informed written consent was obtained for all samples. In short, nucleated cells in the bone marrow aspirates were counted, seeded in tissue culture flasks at a density of 500 000 cells cm−2, and cultured in MSC proliferation medium, consisting of 10% FBS, 100 U mL−1 penicillin, 100 mg mL−1 streptomycin, 1% GlutaMAX, 0.2 × 10−3 m ascorbic acid, and 1 ng mL−1 bFGF (added fresh) in αMEM. Mouse insulinoma MIN6-B1 cells (provided by Dr. P. Halban, University Medical Center, Geneva, Switzerland) were cultured in MIN6 proliferation medium, consisting of 10% (v/v) FBS, 100 U mL−1 penicillin, and 100 mg mL−1 streptomycin, and 71 × 10−6 m 2-mercaptoethanol (added fresh) in DMEM. When cells reached near confluence, the cells were detached using 0.25% Trypsin-EDTA at 37 °C and subsequently subcultured or used for experimentation.

I am at your beck and call to answer questions on our Cell Based Assay Solution. Pete Shuster-CEO and Owner, direct phone: (612) 801-1007 or pshuster@neuromics.com.

Targeted Delivery of Our UCB Derived hMSCs

Liver-targeting Delivery via Intravenous Injection of Cells

Check out how our UCB Human Mesenchymal Stem Cells are engineered for delivery to the liver: Hahn, Sei Kwang (Pohang-si, KR), Kim, Yun Seop (Seoul, KR), Kong, Won Ho (Pohang-si, KR),Kim, Hyemin (Daegu, KR). HYALURONIC ACID DERIVATIVES AND COMPOSITION FOR CELL-SURFACE ENGINEERING USING THE SAME. United States Patent Application 20170067012.

Images: Neuromics' hMSCS in culture

Preparation method of a hyaluronic acid derivative capable of modifying the surface of cells and also having biocompatibility, biodegradability, and liver-targeting deliver property, and use of the hyaluronic acid derivative prepared thereby as a liver-targeting cell delivery system.

Need Cells?

Kick of Your Holiday Season with a 25 USD Gift Card
All cell orders this month will include a 25 USD Amazon Gift Card. This is to help celebrate the Holiday Season.

 Human Astroglia-Neurons Co-Cultures

Here are  your options.
Human Cells

Immortalized Human Brain Microglia Cells

Isolated from healthy human brain tissue

hN2™ Human Neurons Discovery & HT Screening Kits

Stable, Potent and Well Characterized
NeuroNet Pure Human Neurons Discovery and HTS Kits

Pure Population of hPSC Derived Neurons

Human Schwann Cells - New

Human spinal nerve derived primary cells

Human Brain Astrocytes-New

Derived from Cortex

Cancer-Associated Fibroblasts (CAFs) and Cells

Solutions for studying tumor growth, angiogenesis and metastasis.

Human Mesenchymal Stem Cells (hMSCs)

hMSCs Derived from Umbilical Cord Blood
Human Endothelial Cells-New

Artery, Microvascular, Vein &Umbilical Cord Derived
Human Fibroblasts-New

Pancreas and Neonatal Dermal Derived
Human Cardiomyocytes-New

Derived from hMSCs

Mouse and Rat Astrocytes and Neurons

We wish you a Joyous Holiday Season.

Infection of Mesenchymal Stem Cells

UCB derived huMSCs infected with RSVs

Here's cool customer data of our Human Mesenchymal Stem Cells.

Figure. RSV infects and replicates in human MSCs. Human MSCs grown on coverslips, infected with 1 MOI of RSV, and immunostained at 48 or 72 hours post-infection with a monoclonal antibody to RSV tagged with FITC (green), Evan’s blue dye (red) and DAPI (blue). Total magnification of images is 200X (inset 1000X) and scale bar is 50μm (A). RSVN transcripts detected in MSCs at 6, 12, 24 and 72 hours post infection normalized to mock (B). RSV titers (PFU/ml) isolated from the culture medium of infected and mock-infected MSCs is shown (C). Results representative of at least duplicate experiments. doi.org/10.1371/journal.pone.0163709.

Your data is wanted. Just email rose@neuromics.com and claim your 50 USD reward. #datawanted!

Stem Cell "Stemness" and Differentiation States

Markers Play a Key Role

Our Stem Cell Markers are widely used and frequently published. These play an important role for understanding the molecular events that take place during the differentiation of human pluripotent cells. They are essential for the protocols aimed at insuring the generation high of quality differentiated cells.

Here's an excellent study on the determining the state of differentiating stem cells: Julio Castaño, Cristina Morera, Borja Sesé, Stephanie Boue, Carles Bonet-Costa, Merce Martí, Alicia Roque, Albert Jordan, Maria J. Barrero. SETD7 Regulates the Differentiation of Human Embryonic Stem Cells. Published: February 18, 2016DOI: 10.1371/journal.pone.0149502...SOX2 (Neuromics GT15098)...

Figure: SETD7 is expressed at very low levels in pluripotent human cells and induced during differentiation. (A) Average rank of the top 700 most differentially expressed genes between pluripotent (iPSCs or ESCs) and fibroblasts, including those upregulated in pluripotent cells (left panel) and upregulated in fibroblasts (right panel). (B) SETD7 mRNA levels in human ESCs grown under self-renewal conditions (UndES[4]), in vitro differentiated human ESCs (DifES[4]), human fibroblasts (HFF), two lines of human keratinocytes (HEK1 and HEK2) and two lines of iPSCs generated from keratinocytes ([H]KiPS4F and KiPS4F1). Mean and standard deviation of three technical replicates is shown. Induction of SETD7 mRNA levels during ES[4] differentiation was confirmed in more than four independent differentiation experiments. (C) Western blot showing SETD7 protein levels in pluripotent and somatic cells. Loading control beta actin (ACTB) is also shown. (D) Western blot showing protein levels of SETD7, AFP, OCT4 and SOX2 in under self-renewing conditions and in vitro differentiated human ESCs. Loading control alpha tubulin (TUBA) is also shown. One representative experiment out of three is shown. (E) Genomic visualization of the levels of H3K72me3, H3K4me3, H3K4me2, H3K36me3 and RNA polymerase II (Pol II) in the human embryonic stem cell line H1 around the SETD7 gene according to ENCODE. A non-methylated CpG island is depicted in green. (F) Levels of H3K4me2 and H3K27me3 at SETD7 gene promoter region (27 bp upstream of the transcription start site) in pluripotent and somatic cells determined by chromatin immunoprecipitation (ChIP) and ploted relative to the input. IgGs wer used as negative control. Bars show the mean and standard deviation of three independent immunoprecipitations...doi:10.1371/journal.pone.0149502.g001

Neuromics is pleased to provide many different stem cell related options for your cell based assays. I will continue to post updates.

Your Feedback Matters

Our Solutions Must Work

We take our ability to serve you very seriously. We use bird-eye to make sure each and every one of our customers are pleased with results. If you are not happy, we do everything we can to fix your issue. This includes replacement and refunds:

Should you ever have questions or issues, I am at your "beck and call". Do not hesitate to contact me directly. Pete Shuster, CEO and Owner, Neuromics, 612-801-1007 or pshuster@neuromics.com. Thank you!